Standard name
Description Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0.06 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0.06 0.06 0.07 0.09 0.08 0.13 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0.06 0 0 0 0 0 0 0 0.07 0.11 0.18 0.12 0.07 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.1 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0.23 0.19 0.28 0 0 0 0.05 0.05 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0 0 0 0.09 0.06 0 0.08 0.11 0.09 0.13 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.62 0.65 0.33 0.35 0.35 0.34 0.33 0.33 0.41 0.4 0.37 0.39 0.55 0.51 0.46 0.49 0.58 0.55 0 0 0 0 0 0
Peroxisomes 0 0 0.12 0.05 0.06 0.13 0.07 0.08 0 0.05 0.09 0.08 0 0 0 0.07 0.05 0.1 0 0 0 0 0 0
SpindlePole 0.7 0.78 0.87 0.76 0.82 0.76 0.78 0.76 0.77 0.74 0.72 0.73 0.82 0.75 0.64 0.66 0.74 0.74 0.86 0.88 0.88 0.83 0.82 0.77
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 0 1 0 0 0
Bud 1 1 3 2 7 9 21 17 15 15 12 14 2 1 1 0 2 0 5 1 2 0 3 11
Bud Neck 10 5 5 4 2 8 14 12 19 12 15 21 4 4 6 0 6 0 1 0 0 1 1 5
Bud Site 7 0 9 4 8 16 23 20 21 30 26 50 1 4 1 1 1 3 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 5 2 5 13 12 13 15 11 10 7 4 7 24 54 85 27 17 13 0 0 1 1 5 4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 2 0 0 0 0 0 1
Endosome 4 7 28 7 2 5 3 1 4 2 4 2 15 28 33 54 46 54 8 7 9 14 18 24
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 1 0 3 1 2 1
Mitochondria 27 5 8 6 3 26 22 15 23 35 29 50 1 0 4 12 9 2 2 0 1 1 3 5
Nucleus 11 6 9 4 7 17 11 13 5 10 5 17 9 15 30 6 2 5 3 3 0 6 7 8
Nuclear Periphery 1 1 0 2 0 0 0 0 0 3 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 170 159 97 64 66 100 127 113 124 132 115 156 179 243 216 114 143 106 8 7 8 12 12 19
Peroxisomes 6 10 34 9 12 38 27 26 8 15 28 31 5 4 12 17 13 19 3 1 3 3 2 4
SpindlePole 190 191 252 139 155 223 301 260 235 247 226 290 269 362 297 153 182 143 245 223 255 252 308 355
Vac/Vac Membrane 1 0 2 1 0 1 1 0 1 1 1 0 0 3 7 11 8 6 1 1 0 1 4 9
Unique Cell Count 273 246 291 183 188 295 388 342 304 333 313 398 328 481 465 232 245 192 287 253 290 303 378 462
Labelled Cell Count 433 388 452 255 274 456 565 488 465 509 466 640 510 719 692 399 432 353 287 253 290 303 378 462


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 7.3 5.9 5.8 5.9 5.1 5.1 5.1 4.8 4.8 4.3 4.5 6.9 7.0 6.5 9.1 9.4 9.1 7.2 7.0 7.3
Std Deviation (1e-4) 0.9 1.3 1.5 2.2 1.9 1.6 1.6 1.8 1.6 1.4 1.3 1.2 1.2 1.4 1.5 1.9 1.9 1.7 1.3 1.1 1.3
Intensity Change (Log2) – – – -0.02 -0.0 -0.21 -0.22 -0.21 -0.31 -0.29 -0.46 -0.41 0.22 0.24 0.14 0.63 0.66 0.61 0.29 0.24 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1.7 3.1 2.8 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.8 1.5 1.4 0 0 0 0 0 0 0 0 0.6 0
Bud Site -0.6 0.7 1.4 1.7 1.7 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.0 2.7 1.9 1.6 1.2 1.2 0.3 0 0 3.3 4.8 6.8 4.7 3.0 2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -2.3 -3.8 -4.2 -5.5 -5.6 0 0 0 0 -2.5 -2.0 -1.2 4.3 3.1 5.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.3 0 3.1 1.8 1.1 0 0 0 0 0 0 0 1.4 0.6 0
Nucleus -0.6 0.4 1.6 -0.2 0.5 -1.2 -0.1 -1.2 0.8 -0.3 0 2.0 -0.3 -1.9 -0.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.4 0.4 0.1 -0.2 -0.1 0 0 0 0 5.3 4.7 3.6 3.7 5.8 4.8
Peroxisomes -2.5 -1.9 0.4 -2.1 -1.7 0 0 0 0 -5.2 -6.8 -5.1 -1.7 -2.6 -0.6
SpindlePole -3.0 -1.2 -3.4 -3.0 -3.4 0 0 0 0 -1.6 -3.8 -6.8 -5.6 -3.6 -3.4
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0 0