Standard name
Human Ortholog
Description Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0.06 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0.06 0.06 0.07 0.09 0.08 0.13 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0.06 0 0 0 0 0 0 0 0.07 0.11 0.18 0.12 0.07 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.1 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0.23 0.19 0.28 0 0 0 0.05 0.05 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0 0 0 0.09 0.06 0 0.08 0.11 0.09 0.13 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.62 0.65 0.33 0.35 0.35 0.34 0.33 0.33 0.41 0.4 0.37 0.39 0.55 0.51 0.46 0.49 0.58 0.55 0 0 0 0 0 0
Peroxisomes 0 0 0.12 0.05 0.06 0.13 0.07 0.08 0 0.05 0.09 0.08 0 0 0 0.07 0.05 0.1 0 0 0 0 0 0
SpindlePole 0.7 0.78 0.87 0.76 0.82 0.76 0.78 0.76 0.77 0.74 0.72 0.73 0.82 0.75 0.64 0.66 0.74 0.74 0.86 0.88 0.88 0.83 0.82 0.77
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 0 1 0 0 0
Bud 1 1 3 2 7 9 21 17 15 15 12 14 2 1 1 0 2 0 5 1 2 0 3 11
Bud Neck 10 5 5 4 2 8 14 12 19 12 15 21 4 4 6 0 6 0 1 0 0 1 1 5
Bud Site 7 0 9 4 8 16 23 20 21 30 26 50 1 4 1 1 1 3
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 5 2 5 13 12 13 15 11 10 7 4 7 24 54 85 27 17 13 0 0 1 1 5 4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 2 0 0 0 0 0 1
Endosome 4 7 28 7 2 5 3 1 4 2 4 2 15 28 33 54 46 54 8 7 9 14 18 24
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 1 0 3 1 2 1
Mitochondria 27 5 8 6 3 26 22 15 23 35 29 50 1 0 4 12 9 2 2 0 1 1 3 5
Nucleus 11 6 9 4 7 17 11 13 5 10 5 17 9 15 30 6 2 5 3 3 0 6 7 8
Nuclear Periphery 1 1 0 2 0 0 0 0 0 3 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 170 159 97 64 66 100 127 113 124 132 115 156 179 243 216 114 143 106 8 7 8 12 12 19
Peroxisomes 6 10 34 9 12 38 27 26 8 15 28 31 5 4 12 17 13 19 3 1 3 3 2 4
SpindlePole 190 191 252 139 155 223 301 260 235 247 226 290 269 362 297 153 182 143 245 223 255 252 308 355
Vac/Vac Membrane 1 0 2 1 0 1 1 0 1 1 1 0 0 3 7 11 8 6 1 1 0 1 4 9
Unique Cell Count 273 246 291 183 188 295 388 342 304 333 313 398 328 481 465 232 245 192 287 253 290 303 378 462
Labelled Cell Count 433 388 452 255 274 456 565 488 465 509 466 640 510 719 692 399 432 353 287 253 290 303 378 462


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 7.3 5.9 5.8 5.9 5.1 5.1 5.1 4.8 4.8 4.3 4.5 6.9 7.0 6.5 9.1 9.4 9.1 7.2 7.0 7.3
Std Deviation (1e-4) 0.9 1.3 1.5 2.2 1.9 1.6 1.6 1.8 1.6 1.4 1.3 1.2 1.2 1.4 1.5 1.9 1.9 1.7 1.3 1.1 1.3
Intensity Change (Log2) -0.02 -0.0 -0.21 -0.22 -0.21 -0.31 -0.29 -0.46 -0.41 0.22 0.24 0.14 0.63 0.66 0.61 0.29 0.24 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1.7 3.1 2.8 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.8 1.5 1.4 0 0 0 0 0 0 0 0 0.6 0
Bud Site -0.6 0.7 1.4 1.7 1.7 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.0 2.7 1.9 1.6 1.2 1.2 0.3 0 0 3.3 4.8 6.8 4.7 3.0 2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -2.3 -3.8 -4.2 -5.5 -5.6 0 0 0 0 -2.5 -2.0 -1.2 4.3 3.1 5.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.3 0 3.1 1.8 1.1 0 0 0 0 0 0 0 1.4 0.6 0
Nucleus -0.6 0.4 1.6 -0.2 0.5 -1.2 -0.1 -1.2 0.8 -0.3 0 2.0 -0.3 -1.9 -0.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.4 0.4 0.1 -0.2 -0.1 0 0 0 0 5.3 4.7 3.6 3.7 5.8 4.8
Peroxisomes -2.5 -1.9 0.4 -2.1 -1.7 0 0 0 0 -5.2 -6.8 -5.1 -1.7 -2.6 -0.6
SpindlePole -3.0 -1.2 -3.4 -3.0 -3.4 0 0 0 0 -1.6 -3.8 -6.8 -5.6 -3.6 -3.4
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.411 3.3191 3.2526 3.3432 2.9935 3.4479 2.3262 1.9833 1.9769 1.8501 2.04 2.1515 2.0066 1.9805 1.5941 1.9699 1.4273 1.7395
Actin 0.0075 0.0007 0.0062 0 0.0003 0.0002 0.0065 0.0005 0.0049 0 0.0124 0.0002 0.0023 0 0 0 0 0.0021
Bud 0 0.0001 0.0002 0 0 0 0.0002 0.0007 0.0001 0 0.0001 0.0001 0 0.0002 0 0 0 0
Bud Neck 0.0002 0.0003 0.0004 0.011 0.0006 0.0006 0.0012 0.0003 0.0006 0.0011 0.0005 0.0002 0.0001 0.0003 0.0001 0.0036 0.0002 0.0001
Bud Periphery 0.0001 0.0001 0.0002 0 0 0 0.0004 0.0027 0.0002 0 0.0002 0.0003 0 0.0002 0 0 0 0
Bud Site 0.0002 0.0012 0.0017 0 0.0004 0 0.0032 0.0034 0.0008 0.0003 0.0006 0.0001 0.0001 0.0071 0 0.0001 0 0
Cell Periphery 0 0 0.0002 0 0 0 0.0003 0.0001 0 0 0.0001 0 0 0.0001 0 0 0 0
Cytoplasm 0.0005 0.0001 0.0006 0 0.0007 0 0.0017 0 0.0007 0 0.0008 0 0.0001 0.0001 0 0 0 0
Cytoplasmic Foci 0.0172 0.0058 0.0086 0.0071 0.0364 0.0059 0.0391 0.0072 0.012 0.0307 0.0486 0.0039 0.0091 0.0092 0.0039 0.0239 0.0175 0.0011
Eisosomes 0.0002 0 0.0003 0 0 0 0.0001 0 0.0001 0 0.0001 0 0 0 0 0 0 0.0001
Endoplasmic Reticulum 0.0007 0 0.0008 0 0.0002 0 0.0012 0.0001 0.0006 0 0.001 0.0003 0 0 0 0 0 0.0001
Endosome 0.0026 0.0004 0.0032 0.0001 0.0017 0.0004 0.0069 0.0003 0.0063 0.0006 0.0223 0.0014 0.0003 0.0001 0.0001 0.0004 0.0001 0.0004
Golgi 0.0021 0.0007 0.0028 0.0001 0.0012 0.0008 0.0062 0.0003 0.0023 0.001 0.0163 0.0006 0.0003 0.0001 0.0002 0.0005 0.0002 0.0004
Lipid Particles 0.0144 0.0063 0.0059 0.0006 0.0263 0.0072 0.021 0.0013 0.0172 0.003 0.0737 0.0075 0.002 0.0008 0.004 0.0024 0.0079 0.0008
Mitochondria 0.0006 0.002 0.009 0 0 0.0002 0.0107 0.0016 0.0043 0.0357 0.0362 0.013 0.0005 0.0002 0 0 0 0.0012
None 0.0014 0.0001 0.0018 0 0.0006 0 0.0012 0.0001 0.0025 0 0.0021 0.0001 0.0003 0.0001 0 0 0 0.0002
Nuclear Periphery 0.0022 0.0002 0.001 0.0001 0.0012 0.0001 0.0032 0.0008 0.0033 0.0001 0.0022 0.0036 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002
Nucleolus 0.0311 0.0471 0.0709 0.0037 0.0255 0.0204 0.0529 0.0588 0.076 0.0017 0.0111 0.026 0.0209 0.0535 0.0086 0.0029 0.019 0.0119
Nucleus 0.0047 0.0233 0.0144 0.0007 0.0031 0.0032 0.0113 0.0107 0.0119 0.0004 0.001 0.0038 0.0031 0.0325 0.001 0.0007 0.0008 0.0023
Peroxisomes 0.0034 0.0087 0.0039 0.0001 0.0016 0.0023 0.0099 0.0009 0.0041 0.0025 0.0134 0.007 0.0024 0.0002 0.0001 0.0005 0.0002 0.0003
Punctate Nuclear 0.9109 0.9022 0.8669 0.9765 0.8999 0.9587 0.8212 0.9098 0.8506 0.9228 0.7562 0.9313 0.9581 0.8946 0.9816 0.9648 0.954 0.9785
Vacuole 0.0001 0.0005 0.0009 0 0.0001 0 0.001 0.0003 0.0007 0 0.0005 0.0003 0 0.0003 0 0 0 0
Vacuole Periphery 0.0001 0.0001 0.0003 0 0 0 0.0004 0.0002 0.0006 0 0.0006 0.0004 0 0 0 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.1372 19.6402 16.1932 17.8743 6.3301 4.8796 23.3518 23.3311 22.0252 11.6806
Translational Efficiency 0.7184 0.7607 0.8477 0.7853 0.8243 0.7995 0.6989 0.6831 0.5553 0.4977

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1365 564 577 73 1726 1677 413 515 3091 2241 990 588

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 658.98 846.85 991.94 991.43 767.55 849.98 1060.30 1010.09 719.60 849.19 1020.46 1007.77
Standard Deviation 94.56 252.58 118.83 170.56 104.84 114.61 122.72 138.82 113.99 160.90 125.09 143.28
Intensity Change Log 2 0.361872 0.590018 0.589276 0.147168 0.466140 0.396151 0.250332 0.524689 0.488586

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000377 0.000475 0.000057 0.000075 0.000221 0.000373 0.000216 0.000171 0.000290 0.000399 0.000123 0.000159
Bud Neck 0.003489 0.003051 0.000537 0.001757 0.002018 0.002165 0.000925 0.001177 0.002667 0.002388 0.000699 0.001249
Bud Site 0.003875 0.006143 0.000783 0.003403 0.002898 0.002703 0.001339 0.003522 0.003330 0.003569 0.001015 0.003507
Cell Periphery 0.000293 0.000136 0.000011 0.000021 0.000175 0.000093 0.000022 0.000025 0.000227 0.000103 0.000016 0.000024
Cytoplasm 0.015237 0.007453 0.000149 0.000232 0.005267 0.002613 0.000608 0.003747 0.009670 0.003831 0.000340 0.003311
Cytoplasmic Foci 0.050656 0.028221 0.000715 0.006563 0.020362 0.014506 0.003180 0.008977 0.033740 0.017957 0.001743 0.008677
Eisosomes 0.000230 0.000132 0.000009 0.000009 0.000161 0.000076 0.000025 0.000011 0.000192 0.000090 0.000016 0.000010
Endoplasmic Reticulum 0.002241 0.002006 0.000457 0.000137 0.001660 0.000996 0.000698 0.000187 0.001917 0.001250 0.000558 0.000181
Endosome 0.005916 0.005474 0.000164 0.000558 0.002606 0.003434 0.000622 0.003577 0.004068 0.003948 0.000355 0.003202
Golgi 0.011303 0.006833 0.000296 0.000444 0.005508 0.004357 0.000572 0.001463 0.008067 0.004980 0.000411 0.001336
Lipid Particles 0.011953 0.005465 0.000124 0.000428 0.006946 0.006069 0.000855 0.000420 0.009157 0.005917 0.000429 0.000421
Mitochondria 0.002152 0.000389 0.000037 0.000034 0.000523 0.002626 0.000074 0.000152 0.001243 0.002063 0.000053 0.000137
Mitotic Spindle 0.591067* 0.768343* 0.973595* 0.969377* 0.736896* 0.828461* 0.947021* 0.950240* 0.672497* 0.813331* 0.962509* 0.952616*
None 0.015550 0.001334 0.000060 0.000043 0.003554 0.001112 0.000163 0.004212 0.008852 0.001168 0.000103 0.003695
Nuclear Periphery 0.000526 0.000350 0.000083 0.000229 0.000492 0.000345 0.000189 0.000234 0.000507 0.000346 0.000127 0.000234
Nuclear Periphery Foci 0.114316 0.076126 0.004127 0.009979 0.077539 0.058298 0.005077 0.003466 0.093780 0.062784 0.004523 0.004275
Nucleolus 0.105281 0.039310 0.009271 0.002075 0.081979 0.045067 0.012555 0.003217 0.092269 0.043618 0.010641 0.003075
Nucleus 0.030672 0.012450 0.006656 0.000793 0.023734 0.013145 0.018266 0.003009 0.026798 0.012970 0.011500 0.002734
Peroxisomes 0.033276 0.030873 0.001935 0.002945 0.026464 0.012113 0.006258 0.003108 0.029472 0.016835 0.003739 0.003088
Vacuole 0.001345 0.004860 0.000907 0.000864 0.000866 0.001040 0.001281 0.007766 0.001077 0.002001 0.001063 0.006909
Vacuole Periphery 0.000245 0.000574 0.000027 0.000034 0.000132 0.000410 0.000052 0.001319 0.000182 0.000451 0.000038 0.001160

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.40 4.55 0.81 2.53 -1.77 -1.55 -1.92 -2.77 -1.34 0.93 -1.90 -1.05 -2.18 -0.77 -0.42
Bud Neck -2.17 1.59 -2.26 -1.59 -2.61 -0.75 -2.19 -4.11 -3.60 -1.04 -1.28 -0.77 -4.26 -3.54 -3.29
Bud Site -4.35 1.75 -2.86 -1.87 -3.08 -0.41 -2.10 -5.78 -5.67 -4.29 -2.76 -0.63 -6.33 -5.64 -5.97
Cell Periphery 4.38 11.70 7.78 4.12 -3.06 4.56 7.61 7.65 2.69 -0.56 7.04 14.04 11.90 4.52 -2.85
Cytoplasm 1.64 6.29 2.33 1.02 -1.11 2.76 2.65 -2.39 -3.26 -3.39 3.90 7.08 -1.03 -2.73 -3.87
Cytoplasmic Foci 3.28 14.03 5.53 2.85 -2.53 3.06 9.21 2.40 0.33 -3.66 5.99 17.08 5.76 1.61 -4.75
Eisosomes 5.76 17.44 12.64 6.83 -2.19 10.24 16.51 20.59 11.67 3.17 12.14 25.01 24.16 13.96 0.67
Endoplasmic Reticulum 0.42 4.53 7.90 6.20 0.17 7.14 1.73 5.16 1.53 1.76 7.07 4.60 6.97 3.27 0.60
Endosome -0.19 5.52 0.97 1.00 -2.36 -1.14 3.78 -1.06 -0.70 -2.28 -0.08 7.33 -0.36 -0.32 -3.02
Golgi 3.23 13.57 9.73 7.02 -2.67 4.49 14.53 12.49 8.70 -0.26 6.52 19.63 15.85 11.22 -1.82
Lipid Particles 2.97 8.45 7.87 5.87 -3.71 1.69 5.70 6.56 5.26 0.31 3.64 10.49 10.02 7.03 -1.31
Mitochondria 3.50 4.50 4.75 4.00 1.84 -1.43 2.54 1.40 1.63 -1.25 -0.51 5.13 4.32 1.74 -1.54
Mitotic Spindle -8.42 -29.62* -10.72 -5.33 2.15 -9.74 -14.23 -14.99 -7.25 0.04 -14.95 -30.76 -21.10 -9.38 2.68
None 7.70 9.05 9.03 6.92 -0.24 6.40 7.95 0.45 -0.94 -1.32 9.55 11.17 3.20 -0.72 -1.39
Nuclear Periphery 3.93 9.44 1.41 -0.23 -2.03 4.13 2.36 0.42 -1.37 -1.28 5.88 7.45 0.72 -1.41 -2.90
Nuclear Periphery Foci 5.30 23.12* 9.17 5.00 -1.68 6.38 20.51* 23.05* 16.56 1.94 9.29 30.95 29.69 18.09 0.29
Nucleolus 7.92 14.57 15.84 6.59 1.89 6.50 9.18 17.56 11.51 4.47 10.31 16.90 24.73 13.34 4.85
Nucleus 2.79 2.77 4.46 2.28 1.44 3.67 -2.15 0.91 -1.04 2.43 5.01 -0.24 1.85 -0.60 1.55
Peroxisomes -0.02 15.35 7.25 5.73 -2.45 6.15 7.58 12.06 6.77 2.85 6.27 16.20 16.84 9.69 0.77
Vacuole -1.71 -0.75 -1.56 -1.16 -1.46 -0.51 -3.51 -4.33 -4.31 -3.71 -1.65 -2.56 -4.54 -4.35 -4.14
Vacuole Periphery -1.08 2.54 0.22 1.12 -1.35 -1.37 -1.63 -1.50 -1.19 -1.41 -1.59 1.11 -1.45 -1.11 -1.50