Standard name
Description Transcription factor (bHLH) involved in interorganelle communication; contributes to communication between mitochondria, peroxisomes, and nucleus; target of Hog1p; activated in stochastic pulses of nuclear localization
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 1.0 0.79 0.81 0.93 0.87 – 0.83 0.83 0.74 0.77 0.93 0.97 0.94 0.94 0.87 0.89 0.96 0.98 0.96 0.94 0.93 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.05 0.05 0.11 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0.12 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.22 0.21 0.05 0.09 – 0.1 0.1 0.14 0.07 0.09 0 0.1 0.07 0.17 0.17 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 1 1 – 1 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 2 4 2 5 0 1 0 0 0 0 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 6 8 3 5 3 12 – 9 13 3 14 0 1 3 2 3 3 0 0 0 0 0 0
Cytoplasm 177 138 207 175 263 207 366 – 253 249 175 195 230 454 448 214 190 159 173 132 199 129 349 332
Endoplasmic Reticulum 1 2 0 1 6 1 20 – 5 10 2 8 5 3 2 14 4 9 1 0 1 0 5 3
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 2 0 2 0 0 0 0 0
Golgi 0 0 0 0 3 0 0 – 0 0 0 0 1 1 4 3 1 0 1 0 0 0 0 0
Mitochondria 0 0 0 0 0 6 1 – 16 15 27 30 0 0 2 2 1 0 0 0 1 0 0 0
Nucleus 0 0 1 26 4 2 9 – 3 8 6 8 1 5 0 7 5 1 0 0 1 1 5 2
Nuclear Periphery 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 1 0 0 1 0 1 0 0 0 0 0 1 2
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1
Vac/Vac Membrane 2 0 0 48 67 10 37 – 31 31 32 18 22 21 47 16 36 31 0 1 0 0 2 4
Unique Cell Count 179 140 207 222 325 222 422 304 300 236 254 248 470 478 227 218 178 181 135 208 137 374 351
Labelled Cell Count 184 146 216 254 348 230 446 321 330 248 281 259 486 507 258 244 203 181 135 208 137 374 351


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.3 18.0 16.1 15.4 16.3 9.7 11.6 – 9.2 9.2 8.6 8.5 19.4 16.0 18.6 18.8 19.0 19.5 15.8 17.2 18.0
Std Deviation (1e-4) 3.5 3.0 2.2 2.3 2.3 2.2 1.8 – 1.2 1.6 1.1 1.3 3.0 2.5 2.9 4.4 4.4 3.8 2.9 3.0 3.0
Intensity Change (Log2) – – – -0.07 0.02 -0.73 -0.47 – -0.8 -0.81 -0.91 -0.93 0.27 -0.01 0.21 0.23 0.24 0.27 -0.03 0.09 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.6 -1.7 -1.6 -0.7 – -0.6 0.3 -1.8 0.8 0 0 0 0 -1.6 -1.3
Cytoplasm -7.0 -6.7 -3.8 -5.5 – -6.2 -6.3 -7.9 -7.4 -4.0 -2.7 -3.7 -3.5 -5.3 -4.8
Endoplasmic Reticulum 0 0 0 3.2 – 0 0 0 0 0 0 0 3.6 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 3.4 3.3 5.0 5.1 0 0 0 0 0 0
Nucleus 4.8 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 7.1 7.0 0 4.4 – 4.7 4.8 5.5 3.9 4.4 3.1 4.7 3.9 6.1 6.3