Standard name | |
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Description | Transcription factor (bHLH) involved in interorganelle communication; contributes to communication between mitochondria, peroxisomes, and nucleus; target of Hog1p; activated in stochastic pulses of nuclear localization |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 1 | 1 | – | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 4 | 2 | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 6 | 8 | 3 | 5 | 3 | 12 | – | 9 | 13 | 3 | 14 | 0 | 1 | 3 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 177 | 138 | 207 | 175 | 263 | 207 | 366 | – | 253 | 249 | 175 | 195 | 230 | 454 | 448 | 214 | 190 | 159 | 173 | 132 | 199 | 129 | 349 | 332 |
Endoplasmic Reticulum | 1 | 2 | 0 | 1 | 6 | 1 | 20 | – | 5 | 10 | 2 | 8 | 5 | 3 | 2 | 14 | 4 | 9 | 1 | 0 | 1 | 0 | 5 | 3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 3 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 6 | 1 | – | 16 | 15 | 27 | 30 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 1 | 26 | 4 | 2 | 9 | – | 3 | 8 | 6 | 8 | 1 | 5 | 0 | 7 | 5 | 1 | 0 | 0 | 1 | 1 | 5 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Vac/Vac Membrane | 2 | 0 | 0 | 48 | 67 | 10 | 37 | – | 31 | 31 | 32 | 18 | 22 | 21 | 47 | 16 | 36 | 31 | 0 | 1 | 0 | 0 | 2 | 4 |
Unique Cell Count | 179 | 140 | 207 | 222 | 325 | 222 | 422 | 304 | 300 | 236 | 254 | 248 | 470 | 478 | 227 | 218 | 178 | 181 | 135 | 208 | 137 | 374 | 351 | |
Labelled Cell Count | 184 | 146 | 216 | 254 | 348 | 230 | 446 | 321 | 330 | 248 | 281 | 259 | 486 | 507 | 258 | 244 | 203 | 181 | 135 | 208 | 137 | 374 | 351 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 19.3 | 18.0 | 16.1 | 15.4 | 16.3 | 9.7 | 11.6 | – | 9.2 | 9.2 | 8.6 | 8.5 | 19.4 | 16.0 | 18.6 | 18.8 | 19.0 | 19.5 | 15.8 | 17.2 | 18.0 |
Std Deviation (1e-4) | 3.5 | 3.0 | 2.2 | 2.3 | 2.3 | 2.2 | 1.8 | – | 1.2 | 1.6 | 1.1 | 1.3 | 3.0 | 2.5 | 2.9 | 4.4 | 4.4 | 3.8 | 2.9 | 3.0 | 3.0 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.02 | -0.73 | -0.47 | – | -0.8 | -0.81 | -0.91 | -0.93 | 0.27 | -0.01 | 0.21 | 0.23 | 0.24 | 0.27 | -0.03 | 0.09 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.6 | -1.7 | -1.6 | -0.7 | – | -0.6 | 0.3 | -1.8 | 0.8 | 0 | 0 | 0 | 0 | -1.6 | -1.3 |
Cytoplasm | -7.0 | -6.7 | -3.8 | -5.5 | – | -6.2 | -6.3 | -7.9 | -7.4 | -4.0 | -2.7 | -3.7 | -3.5 | -5.3 | -4.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 3.2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 3.4 | 3.3 | 5.0 | 5.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 4.8 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 7.1 | 7.0 | 0 | 4.4 | – | 4.7 | 4.8 | 5.5 | 3.9 | 4.4 | 3.1 | 4.7 | 3.9 | 6.1 | 6.3 |