Standard name | |
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Human Ortholog | |
Description | Transcription factor (bHLH) involved in interorganelle communication; contributes to communication between mitochondria, peroxisomes, and nucleus; target of Hog1p; activated in stochastic pulses of nuclear localization |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 1 | 1 | – | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 4 | 2 | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 6 | 8 | 3 | 5 | 3 | 12 | – | 9 | 13 | 3 | 14 | 0 | 1 | 3 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 177 | 138 | 207 | 175 | 263 | 207 | 366 | – | 253 | 249 | 175 | 195 | 230 | 454 | 448 | 214 | 190 | 159 | 173 | 132 | 199 | 129 | 349 | 332 |
Endoplasmic Reticulum | 1 | 2 | 0 | 1 | 6 | 1 | 20 | – | 5 | 10 | 2 | 8 | 5 | 3 | 2 | 14 | 4 | 9 | 1 | 0 | 1 | 0 | 5 | 3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 3 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 6 | 1 | – | 16 | 15 | 27 | 30 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 1 | 26 | 4 | 2 | 9 | – | 3 | 8 | 6 | 8 | 1 | 5 | 0 | 7 | 5 | 1 | 0 | 0 | 1 | 1 | 5 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Vac/Vac Membrane | 2 | 0 | 0 | 48 | 67 | 10 | 37 | – | 31 | 31 | 32 | 18 | 22 | 21 | 47 | 16 | 36 | 31 | 0 | 1 | 0 | 0 | 2 | 4 |
Unique Cell Count | 179 | 140 | 207 | 222 | 325 | 222 | 422 | 304 | 300 | 236 | 254 | 248 | 470 | 478 | 227 | 218 | 178 | 181 | 135 | 208 | 137 | 374 | 351 | |
Labelled Cell Count | 184 | 146 | 216 | 254 | 348 | 230 | 446 | 321 | 330 | 248 | 281 | 259 | 486 | 507 | 258 | 244 | 203 | 181 | 135 | 208 | 137 | 374 | 351 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 19.3 | 18.0 | 16.1 | 15.4 | 16.3 | 9.7 | 11.6 | – | 9.2 | 9.2 | 8.6 | 8.5 | 19.4 | 16.0 | 18.6 | 18.8 | 19.0 | 19.5 | 15.8 | 17.2 | 18.0 |
Std Deviation (1e-4) | 3.5 | 3.0 | 2.2 | 2.3 | 2.3 | 2.2 | 1.8 | – | 1.2 | 1.6 | 1.1 | 1.3 | 3.0 | 2.5 | 2.9 | 4.4 | 4.4 | 3.8 | 2.9 | 3.0 | 3.0 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.02 | -0.73 | -0.47 | – | -0.8 | -0.81 | -0.91 | -0.93 | 0.27 | -0.01 | 0.21 | 0.23 | 0.24 | 0.27 | -0.03 | 0.09 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.6 | -1.7 | -1.6 | -0.7 | – | -0.6 | 0.3 | -1.8 | 0.8 | 0 | 0 | 0 | 0 | -1.6 | -1.3 |
Cytoplasm | -7.0 | -6.7 | -3.8 | -5.5 | – | -6.2 | -6.3 | -7.9 | -7.4 | -4.0 | -2.7 | -3.7 | -3.5 | -5.3 | -4.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 3.2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 3.4 | 3.3 | 5.0 | 5.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 4.8 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 7.1 | 7.0 | 0 | 4.4 | – | 4.7 | 4.8 | 5.5 | 3.9 | 4.4 | 3.1 | 4.7 | 3.9 | 6.1 | 6.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.0246 | 11.5027 | 10.1652 | 8.6378 | 7.4064 | 10.2546 | 12.2793 | 15.6739 | 14.2939 | 13.2334 | 13.7782 | 14.0841 | 13.916 | 17.217 | 15.5284 | 14.5071 | 13.4842 | 14.9962 |
Actin | 0.0295 | 0.0004 | 0.0043 | 0.004 | 0.0205 | 0.0077 | 0.0186 | 0.0002 | 0.0028 | 0.0006 | 0.0035 | 0.0017 | 0.019 | 0.0149 | 0.0095 | 0.0241 | 0.0553 | 0.0046 |
Bud | 0.0011 | 0.0001 | 0.0003 | 0.0014 | 0.0004 | 0.0003 | 0.0022 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0.0016 | 0.002 | 0.0001 |
Bud Neck | 0.002 | 0.0002 | 0.0007 | 0.0011 | 0.0022 | 0.0031 | 0.0018 | 0.0002 | 0.0005 | 0.0004 | 0.0005 | 0.0014 | 0.0015 | 0.0004 | 0.0043 | 0.0009 | 0.0013 | 0.0014 |
Bud Periphery | 0.0011 | 0 | 0.0002 | 0.0017 | 0.0003 | 0.0004 | 0.0013 | 0.0002 | 0.0001 | 0 | 0.0005 | 0.0002 | 0.0005 | 0 | 0.0006 | 0.0021 | 0.0031 | 0.0001 |
Bud Site | 0.0022 | 0.0003 | 0.0013 | 0.0012 | 0.0008 | 0.0002 | 0.0032 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0013 | 0.001 | 0.0044 | 0.0044 | 0.0158 | 0.0001 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0.0002 | 0.0004 | 0 |
Cytoplasm | 0.7423 | 0.9023 | 0.8773 | 0.8649 | 0.6911 | 0.8997 | 0.8159 | 0.8821 | 0.8719 | 0.8509 | 0.9158 | 0.8637 | 0.8201 | 0.9184 | 0.8502 | 0.7646 | 0.7343 | 0.8937 |
Cytoplasmic Foci | 0.025 | 0.0008 | 0.015 | 0.0054 | 0.0239 | 0.0034 | 0.0167 | 0.0007 | 0.005 | 0.0103 | 0.0015 | 0.003 | 0.0208 | 0.0008 | 0.0034 | 0.0182 | 0.0275 | 0.0045 |
Eisosomes | 0.0009 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0034 | 0.0011 | 0.0018 | 0.0042 | 0.0045 | 0.0029 | 0.0054 | 0.0007 | 0.0015 | 0.0027 | 0.0023 | 0.0037 | 0.01 | 0.0008 | 0.0012 | 0.0007 | 0.0051 | 0.003 |
Endosome | 0.0189 | 0.0004 | 0.0084 | 0.0183 | 0.0191 | 0.0088 | 0.0156 | 0.0002 | 0.0031 | 0.0187 | 0.0132 | 0.0061 | 0.0179 | 0.0003 | 0.0069 | 0.0182 | 0.0588 | 0.0051 |
Golgi | 0.0134 | 0 | 0.0029 | 0.0064 | 0.0269 | 0.0054 | 0.0029 | 0 | 0.0006 | 0.002 | 0.0028 | 0.0017 | 0.0055 | 0.0001 | 0.0026 | 0.0178 | 0.0201 | 0.0018 |
Lipid Particles | 0.0205 | 0 | 0.0025 | 0.0036 | 0.0204 | 0.0071 | 0.0031 | 0 | 0.0043 | 0.0012 | 0.0032 | 0.0005 | 0.0031 | 0 | 0.0034 | 0.0196 | 0.0107 | 0.0003 |
Mitochondria | 0.0247 | 0.0001 | 0.0048 | 0.0453 | 0.0428 | 0.0061 | 0.0038 | 0.0061 | 0.0016 | 0.0003 | 0.0057 | 0.001 | 0.0048 | 0.0001 | 0.0012 | 0.0079 | 0.0108 | 0.0087 |
None | 0.0024 | 0.0009 | 0.0009 | 0.0017 | 0.0038 | 0.0008 | 0.0082 | 0.0016 | 0.0016 | 0.0009 | 0.0012 | 0.0016 | 0.0031 | 0.0007 | 0.0019 | 0.0007 | 0.0024 | 0.0007 |
Nuclear Periphery | 0.0103 | 0.0061 | 0.009 | 0.0054 | 0.0085 | 0.0037 | 0.0117 | 0.0033 | 0.005 | 0.0041 | 0.0234 | 0.0064 | 0.0124 | 0.0038 | 0.0038 | 0.0028 | 0.0053 | 0.0042 |
Nucleolus | 0.0005 | 0.0001 | 0.0002 | 0.0005 | 0.0009 | 0.0001 | 0.0006 | 0.0004 | 0.0016 | 0 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0006 | 0.0007 | 0.0005 | 0 |
Nucleus | 0.0811 | 0.0845 | 0.0576 | 0.0227 | 0.0181 | 0.0466 | 0.0752 | 0.102 | 0.0906 | 0.1049 | 0.0198 | 0.1053 | 0.0665 | 0.0573 | 0.0878 | 0.0874 | 0.0218 | 0.0625 |
Peroxisomes | 0.0136 | 0 | 0.0083 | 0.0015 | 0.0718 | 0.0005 | 0.0027 | 0.0001 | 0.0043 | 0.0004 | 0.0005 | 0.0001 | 0.0037 | 0.0001 | 0.0019 | 0.0226 | 0.0074 | 0.0003 |
Punctate Nuclear | 0.0033 | 0.0021 | 0.0017 | 0.001 | 0.042 | 0.0006 | 0.0057 | 0.0012 | 0.0038 | 0.0013 | 0.0014 | 0.0018 | 0.0014 | 0.0008 | 0.0113 | 0.0012 | 0.0028 | 0.0084 |
Vacuole | 0.0024 | 0.0004 | 0.0024 | 0.0065 | 0.0009 | 0.0019 | 0.0037 | 0.0003 | 0.0006 | 0.0007 | 0.0011 | 0.001 | 0.0059 | 0.0003 | 0.0033 | 0.0033 | 0.0102 | 0.0002 |
Vacuole Periphery | 0.0011 | 0.0001 | 0.0005 | 0.0029 | 0.0006 | 0.0006 | 0.0011 | 0.0001 | 0.0002 | 0.0002 | 0.0031 | 0.0002 | 0.0014 | 0.0001 | 0.0005 | 0.001 | 0.0041 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.4973 | 24.0129 | 27.4132 | 26.8248 | 36.1672 | 12.3909 | 30.8162 | 31.5134 | 28.9515 | 22.5202 |
Translational Efficiency | 1.7116 | 1.3309 | 1.3489 | 1.4208 | 0.7155 | 2.6507 | 1.2772 | 1.6737 | 1.6023 | 1.2773 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |