Standard name
Human Ortholog
Description Transcription factor (bHLH) involved in interorganelle communication; contributes to communication between mitochondria, peroxisomes, and nucleus; target of Hog1p; activated in stochastic pulses of nuclear localization
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 1.0 0.79 0.81 0.93 0.87 – 0.83 0.83 0.74 0.77 0.93 0.97 0.94 0.94 0.87 0.89 0.96 0.98 0.96 0.94 0.93 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.05 0.05 0.11 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0.12 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.22 0.21 0.05 0.09 – 0.1 0.1 0.14 0.07 0.09 0 0.1 0.07 0.17 0.17 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 1 1 – 1 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 2 4 2 5 0 1 0 0 0 0 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 6 8 3 5 3 12 – 9 13 3 14 0 1 3 2 3 3 0 0 0 0 0 0
Cytoplasm 177 138 207 175 263 207 366 – 253 249 175 195 230 454 448 214 190 159 173 132 199 129 349 332
Endoplasmic Reticulum 1 2 0 1 6 1 20 – 5 10 2 8 5 3 2 14 4 9 1 0 1 0 5 3
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 2 0 2 0 0 0 0 0
Golgi 0 0 0 0 3 0 0 – 0 0 0 0 1 1 4 3 1 0 1 0 0 0 0 0
Mitochondria 0 0 0 0 0 6 1 – 16 15 27 30 0 0 2 2 1 0 0 0 1 0 0 0
Nucleus 0 0 1 26 4 2 9 – 3 8 6 8 1 5 0 7 5 1 0 0 1 1 5 2
Nuclear Periphery 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 1 0 0 1 0 1 0 0 0 0 0 1 2
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1
Vac/Vac Membrane 2 0 0 48 67 10 37 – 31 31 32 18 22 21 47 16 36 31 0 1 0 0 2 4
Unique Cell Count 179 140 207 222 325 222 422 304 300 236 254 248 470 478 227 218 178 181 135 208 137 374 351
Labelled Cell Count 184 146 216 254 348 230 446 321 330 248 281 259 486 507 258 244 203 181 135 208 137 374 351


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.3 18.0 16.1 15.4 16.3 9.7 11.6 – 9.2 9.2 8.6 8.5 19.4 16.0 18.6 18.8 19.0 19.5 15.8 17.2 18.0
Std Deviation (1e-4) 3.5 3.0 2.2 2.3 2.3 2.2 1.8 – 1.2 1.6 1.1 1.3 3.0 2.5 2.9 4.4 4.4 3.8 2.9 3.0 3.0
Intensity Change (Log2) – – – -0.07 0.02 -0.73 -0.47 – -0.8 -0.81 -0.91 -0.93 0.27 -0.01 0.21 0.23 0.24 0.27 -0.03 0.09 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.6 -1.7 -1.6 -0.7 – -0.6 0.3 -1.8 0.8 0 0 0 0 -1.6 -1.3
Cytoplasm -7.0 -6.7 -3.8 -5.5 – -6.2 -6.3 -7.9 -7.4 -4.0 -2.7 -3.7 -3.5 -5.3 -4.8
Endoplasmic Reticulum 0 0 0 3.2 – 0 0 0 0 0 0 0 3.6 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 3.4 3.3 5.0 5.1 0 0 0 0 0 0
Nucleus 4.8 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 7.1 7.0 0 4.4 – 4.7 4.8 5.5 3.9 4.4 3.1 4.7 3.9 6.1 6.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.0246 11.5027 10.1652 8.6378 7.4064 10.2546 12.2793 15.6739 14.2939 13.2334 13.7782 14.0841 13.916 17.217 15.5284 14.5071 13.4842 14.9962
Actin 0.0295 0.0004 0.0043 0.004 0.0205 0.0077 0.0186 0.0002 0.0028 0.0006 0.0035 0.0017 0.019 0.0149 0.0095 0.0241 0.0553 0.0046
Bud 0.0011 0.0001 0.0003 0.0014 0.0004 0.0003 0.0022 0.0002 0.0003 0.0001 0.0002 0.0003 0.0004 0.0001 0.0004 0.0016 0.002 0.0001
Bud Neck 0.002 0.0002 0.0007 0.0011 0.0022 0.0031 0.0018 0.0002 0.0005 0.0004 0.0005 0.0014 0.0015 0.0004 0.0043 0.0009 0.0013 0.0014
Bud Periphery 0.0011 0 0.0002 0.0017 0.0003 0.0004 0.0013 0.0002 0.0001 0 0.0005 0.0002 0.0005 0 0.0006 0.0021 0.0031 0.0001
Bud Site 0.0022 0.0003 0.0013 0.0012 0.0008 0.0002 0.0032 0.0002 0.0005 0.0001 0.0001 0.0002 0.0013 0.001 0.0044 0.0044 0.0158 0.0001
Cell Periphery 0.0002 0 0.0001 0.0003 0.0001 0.0001 0.0003 0 0 0 0 0 0.0001 0 0.0004 0.0002 0.0004 0
Cytoplasm 0.7423 0.9023 0.8773 0.8649 0.6911 0.8997 0.8159 0.8821 0.8719 0.8509 0.9158 0.8637 0.8201 0.9184 0.8502 0.7646 0.7343 0.8937
Cytoplasmic Foci 0.025 0.0008 0.015 0.0054 0.0239 0.0034 0.0167 0.0007 0.005 0.0103 0.0015 0.003 0.0208 0.0008 0.0034 0.0182 0.0275 0.0045
Eisosomes 0.0009 0 0 0.0001 0.0002 0.0001 0.0003 0 0 0 0 0 0.0002 0 0.0001 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.0034 0.0011 0.0018 0.0042 0.0045 0.0029 0.0054 0.0007 0.0015 0.0027 0.0023 0.0037 0.01 0.0008 0.0012 0.0007 0.0051 0.003
Endosome 0.0189 0.0004 0.0084 0.0183 0.0191 0.0088 0.0156 0.0002 0.0031 0.0187 0.0132 0.0061 0.0179 0.0003 0.0069 0.0182 0.0588 0.0051
Golgi 0.0134 0 0.0029 0.0064 0.0269 0.0054 0.0029 0 0.0006 0.002 0.0028 0.0017 0.0055 0.0001 0.0026 0.0178 0.0201 0.0018
Lipid Particles 0.0205 0 0.0025 0.0036 0.0204 0.0071 0.0031 0 0.0043 0.0012 0.0032 0.0005 0.0031 0 0.0034 0.0196 0.0107 0.0003
Mitochondria 0.0247 0.0001 0.0048 0.0453 0.0428 0.0061 0.0038 0.0061 0.0016 0.0003 0.0057 0.001 0.0048 0.0001 0.0012 0.0079 0.0108 0.0087
None 0.0024 0.0009 0.0009 0.0017 0.0038 0.0008 0.0082 0.0016 0.0016 0.0009 0.0012 0.0016 0.0031 0.0007 0.0019 0.0007 0.0024 0.0007
Nuclear Periphery 0.0103 0.0061 0.009 0.0054 0.0085 0.0037 0.0117 0.0033 0.005 0.0041 0.0234 0.0064 0.0124 0.0038 0.0038 0.0028 0.0053 0.0042
Nucleolus 0.0005 0.0001 0.0002 0.0005 0.0009 0.0001 0.0006 0.0004 0.0016 0 0.0002 0.0001 0.0003 0 0.0006 0.0007 0.0005 0
Nucleus 0.0811 0.0845 0.0576 0.0227 0.0181 0.0466 0.0752 0.102 0.0906 0.1049 0.0198 0.1053 0.0665 0.0573 0.0878 0.0874 0.0218 0.0625
Peroxisomes 0.0136 0 0.0083 0.0015 0.0718 0.0005 0.0027 0.0001 0.0043 0.0004 0.0005 0.0001 0.0037 0.0001 0.0019 0.0226 0.0074 0.0003
Punctate Nuclear 0.0033 0.0021 0.0017 0.001 0.042 0.0006 0.0057 0.0012 0.0038 0.0013 0.0014 0.0018 0.0014 0.0008 0.0113 0.0012 0.0028 0.0084
Vacuole 0.0024 0.0004 0.0024 0.0065 0.0009 0.0019 0.0037 0.0003 0.0006 0.0007 0.0011 0.001 0.0059 0.0003 0.0033 0.0033 0.0102 0.0002
Vacuole Periphery 0.0011 0.0001 0.0005 0.0029 0.0006 0.0006 0.0011 0.0001 0.0002 0.0002 0.0031 0.0002 0.0014 0.0001 0.0005 0.001 0.0041 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.4973 24.0129 27.4132 26.8248 36.1672 12.3909 30.8162 31.5134 28.9515 22.5202
Translational Efficiency 1.7116 1.3309 1.3489 1.4208 0.7155 2.6507 1.2772 1.6737 1.6023 1.2773

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘