Standard name
Human Ortholog
Description Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.96 0.97 0.94 0.94 0.88 – 0.83 0.75 0.68 0.7 0.99 0.99 0.99 0.94 – – 0.96 0.95 0.9 0.94 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.1 0.06 0.31 0.21 0 0 0 0 – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0.05 0.05 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0 0.05 0.05 0.09 – 0.06 0.18 0.09 0.12 0 0 0 0.06 – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 – 0 2 2 1 0 0 0 0 – – 0 2 0 0 0 1
Bud 0 0 1 0 0 0 0 – 1 2 1 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 2 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 4 1 3 0 3 4 – 2 6 5 8 2 0 1 0 – – 0 0 0 0 0 0
Cytoplasm 149 292 202 216 206 205 266 – 184 247 154 194 168 290 246 17 – – 149 277 199 158 164 269
Endoplasmic Reticulum 0 3 2 1 3 2 2 – 1 4 3 5 1 1 4 0 – – 0 0 3 0 2 2
Endosome 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 1 1 0 0 1
Golgi 1 1 0 0 0 1 0 – 0 0 0 0 0 0 0 1 – – 1 1 0 1 1 0
Mitochondria 1 1 0 0 0 4 10 – 22 21 70 58 0 0 1 0 – – 1 1 2 1 1 0
Nucleus 0 2 0 2 2 3 7 – 10 15 7 9 0 1 2 0 – – 0 0 2 0 1 1
Nuclear Periphery 0 0 0 0 0 0 1 – 0 1 1 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 1 0 0 0 1 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 1 0 0 0 0
Vac/Vac Membrane 0 1 10 5 11 11 26 – 14 58 20 32 0 0 2 1 – – 1 1 3 0 0 2
Unique Cell Count 151 297 210 222 219 218 304 221 331 228 277 169 293 249 18 155 291 221 168 177 286
Labelled Cell Count 151 304 216 228 222 229 317 234 357 263 307 171 293 256 19 155 291 221 168 177 286


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 12.0 9.7 10.0 9.5 8.4 8.5 – 7.4 7.5 6.7 6.9 11.9 11.7 11.5 13.7 – – 8.9 9.5 9.8
Std Deviation (1e-4) 1.3 1.7 1.3 1.8 1.3 1.3 1.8 – 1.4 1.4 1.4 1.3 1.8 2.1 2.0 2.7 – – 1.5 1.6 1.8
Intensity Change (Log2) – – – 0.04 -0.04 -0.21 -0.19 – -0.39 -0.38 -0.54 -0.49 0.29 0.27 0.25 0.5 – – -0.12 -0.03 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Cytoplasm 0.6 -1.0 -1.0 -3.4 – -4.4 -6.5 -7.7 -7.3 2.0 2.1 1.8 -0.4 – –
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Mitochondria 0 0 0 0 – 4.7 3.7 8.8 7.1 0 0 0 0 – –
Nucleus 0 0 0 0 – 0 3.1 0 0 0 0 0 0 – –
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Vacuole -1.4 0.1 0.1 1.7 – 0.7 4.4 1.7 2.6 0 0 -2.6 0 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9155 5.7499 4.7794 4.0589 4.3647 5.1927 4.8577 6.2722 5.614 5.6478 4.9982 5.7482 5.8298 7.8108 6.6123 6.4106 7.1141 7.0002
Actin 0.0387 0.0001 0.0083 0.0416 0.0002 0.0003 0.007 0.0005 0.011 0.0241 0.0085 0.0021 0.0336 0.0006 0.0124 0.0107 0.0322 0.0013
Bud 0.0005 0.0001 0.0021 0.0009 0.0004 0.0001 0.0003 0.0027 0.0004 0.0004 0.0015 0.0016 0.0004 0.0017 0.0002 0.0005 0.0002 0.0001
Bud Neck 0.0007 0.0001 0.0015 0.0006 0.0007 0.0007 0.0058 0.0002 0.0008 0.0008 0.0011 0.0011 0.0013 0.0001 0.0005 0.0008 0.0005 0.0008
Bud Periphery 0.0008 0 0.0022 0.0011 0.0002 0.0001 0.0003 0.0005 0.0003 0.0003 0.0005 0.0006 0.0003 0.0003 0.0002 0.0003 0.0001 0.0001
Bud Site 0.0014 0.0002 0.0021 0.0025 0.0003 0.0002 0.0014 0.003 0.0014 0.0006 0.0018 0.0004 0.0009 0.0033 0.001 0.003 0.0001 0.0001
Cell Periphery 0.0002 0 0.0003 0.0002 0 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0.0002 0.0001 0 0
Cytoplasm 0.8271 0.9851 0.9047 0.8211 0.867 0.9187 0.8641 0.9564 0.8927 0.8789 0.8823 0.9478 0.8317 0.9753 0.951 0.9235 0.9435 0.9711
Cytoplasmic Foci 0.0214 0.0015 0.0124 0.014 0.0123 0.0041 0.0263 0.0063 0.0192 0.0181 0.0198 0.0117 0.034 0.0048 0.006 0.0047 0.0046 0.0039
Eisosomes 0.0003 0 0.0001 0.0004 0 0 0.0001 0 0.0001 0.0001 0 0 0.0004 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0047 0.0007 0.0056 0.0011 0.001 0.0022 0.0033 0.001 0.0019 0.0013 0.0011 0.0014 0.0064 0.0005 0.0036 0.0054 0.0015 0.0014
Endosome 0.0179 0.0008 0.0115 0.0202 0.0867 0.021 0.0276 0.0061 0.0151 0.0142 0.0308 0.0063 0.0191 0.0008 0.0056 0.0164 0.0017 0.0041
Golgi 0.0162 0.0001 0.0054 0.0195 0.0171 0.0052 0.0102 0.0025 0.0052 0.0154 0.0267 0.0021 0.0077 0.0001 0.0026 0.0011 0.0008 0.0003
Lipid Particles 0.0098 0 0.0078 0.0045 0.0014 0.0005 0.0052 0.0001 0.0017 0.0033 0.0044 0.0033 0.0104 0.0001 0.0033 0.0014 0.0001 0.0001
Mitochondria 0.0207 0 0.0222 0.0535 0.0016 0.0074 0.0021 0.0074 0.0055 0.005 0.0031 0.0016 0.0034 0.0008 0.0007 0.0009 0.0003 0.0006
None 0.0172 0.0091 0.0035 0.0043 0.0019 0.0257 0.0124 0.0065 0.0187 0.0069 0.0019 0.0054 0.014 0.0034 0.0035 0.0036 0.0062 0.0017
Nuclear Periphery 0.0022 0.0003 0.0014 0.0007 0.0006 0.0016 0.0106 0.0007 0.0025 0.0012 0.0009 0.001 0.0093 0.001 0.0011 0.0073 0.0009 0.0031
Nucleolus 0.0002 0 0.0002 0.0001 0 0.0002 0.0007 0 0.0009 0.0001 0 0.0001 0.0003 0.0002 0 0.0005 0 0.0001
Nucleus 0.0022 0.0014 0.0023 0.003 0.0029 0.0063 0.0069 0.004 0.0097 0.0206 0.0079 0.0068 0.0095 0.0053 0.004 0.0094 0.0057 0.0092
Peroxisomes 0.0142 0 0.002 0.0084 0.0004 0.0001 0.0057 0.0006 0.0053 0.0054 0.0055 0.0015 0.0117 0.0001 0.0027 0.0004 0.0005 0.0001
Punctate Nuclear 0.0009 0.0001 0.0003 0.0004 0.0006 0.0009 0.0036 0.0007 0.0047 0.0023 0.0007 0.0043 0.0034 0.001 0.0006 0.0053 0.0002 0.0011
Vacuole 0.0016 0.0003 0.0031 0.0014 0.0031 0.0034 0.0045 0.0005 0.0022 0.0009 0.0009 0.0006 0.0012 0.0004 0.0006 0.0036 0.0006 0.0006
Vacuole Periphery 0.001 0 0.001 0.0006 0.0016 0.0012 0.0017 0.0002 0.0006 0.0003 0.0005 0.0003 0.0009 0.0001 0.0001 0.0009 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.376 44.5491 62.5216 70.589 35.1157 37.3553 49.2052 58.9853 58.2161 39.7707
Translational Efficiency 0.4673 0.5183 0.5405 0.5348 0.4205 0.5181 0.5448 0.3716 0.4599 0.3863

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1966 1294 250 1212 2417 1937 129 146 4383 3231 379 1358

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 840.84 885.86 1723.07 1011.81 986.59 1025.81 1229.15 1108.75 921.21 969.76 1554.95 1022.23
Standard Deviation 112.05 172.92 223.07 140.37 140.34 156.70 136.05 139.63 147.47 177.20 306.43 143.47
Intensity Change Log 2 — 0.075247 1.035078 0.267035 — 0.056241 0.317138 0.168412 — 0.065017 0.691984 0.214629

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000064 0.000078 0.000691 0.000947 0.000088 0.000239 0.000654 0.014864 0.000077 0.000174 0.000678 0.002444
Bud Neck 0.002684 0.001427 0.003032 0.014614 0.001048 0.001982 0.002050 0.013837 0.001782 0.001760 0.002698 0.014530
Bud Site 0.003923 0.003192 0.003923 0.077615 0.002533 0.009063 0.006458 0.145766* 0.003156 0.006712 0.004786 0.084942
Cell Periphery 0.000069 0.000053 0.000249 0.000150 0.000032 0.000046 0.000230 0.000286 0.000048 0.000049 0.000243 0.000164
Cytoplasm 0.782113* 0.829387* 0.431145* 0.528179* 0.819848* 0.798585* 0.402879* 0.301829* 0.802922* 0.810921* 0.421524* 0.503844*
Cytoplasmic Foci 0.062474 0.030480 0.007367 0.032405 0.048968 0.056695 0.021269 0.105284 0.055026 0.046196 0.012099 0.040240
Eisosomes 0.000025 0.000008 0.000013 0.000033 0.000007 0.000012 0.000064 0.000079 0.000015 0.000010 0.000030 0.000038
Endoplasmic Reticulum 0.000394 0.000561 0.001391 0.001294 0.000950 0.000513 0.004984 0.001126 0.000701 0.000532 0.002614 0.001276
Endosome 0.002646 0.001829 0.001439 0.011649 0.001750 0.004095 0.005619 0.025937 0.002152 0.003188 0.002862 0.013185
Golgi 0.000508 0.000395 0.000367 0.000970 0.000461 0.002324 0.000192 0.014786 0.000482 0.001552 0.000308 0.002455
Lipid Particles 0.000705 0.000417 0.001420 0.001022 0.000504 0.000825 0.002084 0.000642 0.000594 0.000662 0.001646 0.000981
Mitochondria 0.000787 0.000227 0.000453 0.001165 0.000832 0.000952 0.000171 0.001085 0.000812 0.000662 0.000357 0.001157
Mitotic Spindle 0.003486 0.001773 0.015927 0.068933 0.001939 0.009131 0.000915 0.099312 0.002633 0.006184 0.010818 0.072199
None 0.009075 0.008406 0.007577 0.006264 0.004193 0.003333 0.001740 0.001312 0.006383 0.005365 0.005590 0.005731
Nuclear Periphery 0.000118 0.000171 0.001354 0.000656 0.000155 0.000193 0.001351 0.000309 0.000138 0.000184 0.001353 0.000619
Nuclear Periphery Foci 0.001126 0.001698 0.002921 0.003029 0.001271 0.001322 0.011885 0.001211 0.001206 0.001473 0.005972 0.002833
Nucleolus 0.000484 0.000784 0.000191 0.000518 0.000229 0.000299 0.000170 0.000282 0.000343 0.000493 0.000184 0.000492
Nucleus 0.076383 0.079033 0.435420* 0.098233 0.073950 0.043477 0.220552* 0.040803 0.075041 0.057717 0.362286* 0.092059
Peroxisomes 0.000464 0.000285 0.000094 0.006610 0.000282 0.001005 0.000260 0.082261 0.000363 0.000717 0.000151 0.014743
Vacuole 0.052116 0.039198 0.084842 0.144705 0.040596 0.065090 0.316275* 0.148554 0.045763 0.054720 0.163615 0.145119
Vacuole Periphery 0.000356 0.000599 0.000182 0.001009 0.000365 0.000818 0.000198 0.000435 0.000361 0.000730 0.000187 0.000948

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.86 -13.79 -5.28 -5.15 3.04 -2.86 -6.87 -1.24 -1.14 -0.92 -3.16 -14.58 -4.11 -3.62 0.61
Bud Neck 1.97 -5.26 -6.87 -8.62 -1.05 -3.70 -4.91 -4.79 -4.14 -3.76 0.01 -6.59 -9.13 -9.26 -3.04
Bud Site 0.89 -5.44 -9.98 -10.25 -8.48 -5.84 -5.92 -4.40 -3.80 -3.96 -4.78 -8.43 -11.17 -9.99 -9.50
Cell Periphery 1.19 -12.00 -5.12 -5.14 10.34 -4.42 -9.12 -3.24 -2.68 2.65 -0.06 -13.67 -8.56 -7.95 10.31
Cytoplasm -5.45 34.08 38.64 40.24 -7.87 5.05 24.13 19.76 18.31 -0.22 0.43 42.68 46.68 44.44 -7.75
Cytoplasmic Foci 10.53 22.57 14.11 2.90 -7.01 -2.82 10.47 1.29 2.35 -3.15 3.86 24.01 12.99 8.98 -6.85
Eisosomes 6.59 -2.06 -5.43 -15.90 -3.82 -4.79 -11.74 -5.97 -5.20 2.02 3.80 -11.09 -13.02 -16.91 0.05
Endoplasmic Reticulum -2.02 -10.41 -10.89 -8.76 4.73 1.68 -5.91 0.09 -3.27 6.67 1.14 -9.26 -4.41 -10.16 7.32
Endosome 3.22 -0.79 -3.39 -4.32 -3.10 -7.55 -3.03 -2.57 -1.39 -0.85 -4.89 -3.57 -4.54 -3.09 -2.38
Golgi 1.06 -0.87 0.49 -0.81 2.25 -3.55 6.20 -1.08 0.11 -1.28 -3.31 0.30 -0.76 2.53 -0.91
Lipid Particles 3.30 -11.53 -8.13 -13.19 8.95 -1.48 -9.24 -5.75 -2.59 2.08 -0.53 -13.59 -12.48 -5.74 9.05
Mitochondria 2.09 0.42 0.67 -3.24 0.68 -0.16 1.44 1.28 2.28 -0.65 0.55 1.10 0.88 0.41 -0.44
Mitotic Spindle 1.59 -2.97 -9.02 -9.63 -6.47 -4.35 -0.19 -3.83 -3.31 -3.82 -3.05 -3.22 -10.10 -9.23 -8.20
None 1.04 5.56 4.36 2.70 -1.37 1.75 3.03 -0.37 -1.24 -1.87 2.28 5.15 1.95 -0.06 -2.37
Nuclear Periphery -2.47 -18.73 -21.59 -21.08 9.96 -1.07 -13.53 -7.93 -7.09 3.67 -2.00 -21.22 -23.39 -20.41 9.61
Nuclear Periphery Foci -2.76 -11.16 -6.53 -3.62 6.08 -0.11 -8.35 -3.13 -2.86 6.31 -1.63 -13.36 -6.75 -5.06 8.44
Nucleolus -1.50 2.39 -2.18 0.27 -3.66 -1.70 2.20 -2.65 -2.23 -3.03 -1.80 1.79 -4.20 -1.99 -4.86
Nucleus -1.52 -20.35 -13.32 -11.02 15.19 14.02 -6.83 -0.20 -3.88 6.28 8.00 -19.57 -13.65 -17.03 14.00
Peroxisomes 0.96 2.49 -5.48 -5.76 -6.07 -4.50 0.07 -2.41 -2.28 -2.41 -3.30 2.15 -5.51 -5.01 -5.70
Vacuole 1.93 -13.80 -27.71 -27.88 -1.32 -9.71 -14.12 -12.99 -11.71 0.28 -6.70 -19.25 -31.40 -28.66 0.92
Vacuole Periphery -1.71 1.23 0.23 1.72 -0.58 -3.15 4.95 3.09 4.49 -0.92 -3.58 3.43 0.57 3.41 -1.10
Standard name
Human Ortholog
Description Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gsh1

Gsh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gsh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available