Standard name
Description Bifunctional DRAP deaminase tRNA:pseudouridine synthase; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs; RIB2 has a paralog, PUS9, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0.06 0.05 0 0 0 – 0 0
Cytoplasm 0.95 0.98 0.9 0.98 0.98 0.94 0.9 0.91 0.74 0.75 0.61 0.7 – – – 0.96 0.97 0.98 0.88 0.89 0.73 – 0.75 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0.12 0
Mitochondria 0 0 0.05 0 0 0.1 0.1 0.09 0.35 0.22 0.52 0.39 – – – 0 0 0 0 0 0.11 – 0 0.07
Nucleus 0 0 0.08 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 2 0 1 0 0 – – – 0 0 0 0 1 0 – 0 0
Bud 1 1 0 1 0 0 2 2 4 2 5 4 – – – 0 0 0 1 8 0 – 0 0
Bud Neck 0 0 0 2 1 0 0 6 3 1 0 1 – – – 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 4 0 2 4 6 3 4 2 5 2 2 – – – 6 18 14 0 0 0 – 0 0
Cytoplasm 142 299 35 113 183 164 209 328 116 126 117 124 – – – 299 311 294 132 267 49 – 5 12
Endoplasmic Reticulum 2 4 0 0 0 1 2 2 1 4 2 0 – – – 12 11 9 1 1 1 – 0 0
Endosome 0 0 0 0 0 0 0 0 1 1 0 4 – – – 1 0 1 3 2 1 – 0 0
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 – – – 0 1 0 0 2 0 – 0 0
Mitochondria 6 1 2 0 1 18 22 32 54 37 100 69 – – – 7 4 1 4 4 7 – 0 1
Nucleus 0 1 3 0 1 1 0 5 3 4 0 0 – – – 2 5 4 0 0 1 – 0 0
Nuclear Periphery 0 2 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 – – – 1 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 1 1 0 0 1 1 – – – 1 0 0 1 3 0 – 0 0
Vac/Vac Membrane 0 2 0 1 2 1 4 4 4 7 4 7 – – – 8 4 2 0 3 0 – 0 0
Unique Cell Count 150 306 39 115 186 174 231 362 156 168 192 178 312 320 301 150 299 68 8 16
Labelled Cell Count 152 315 40 119 192 192 244 386 188 188 231 212 337 354 325 150 299 68 8 16


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 6.6 5.8 6.5 6.8 5.6 5.7 5.6 4.6 4.8 4.1 5.1 – – – 7.9 8.0 7.7 – – –
Std Deviation (1e-4) 1.5 2.1 1.5 1.7 2.0 2.2 1.8 2.0 1.8 1.6 1.1 2.1 – – – 1.5 1.4 1.3 – – –
Intensity Change (Log2) – – – 0.16 0.23 -0.05 -0.03 -0.05 -0.33 -0.28 -0.49 -0.19 – – – 0.44 0.47 0.41 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cytoplasm 2.4 2.8 1.0 0.1 0.2 -2.1 -2.0 -3.5 -2.6 – – – 1.7 2.3 2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 4.1 – – – 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – – – 0 0 0