Standard name
Human Ortholog
Description Bifunctional DRAP deaminase tRNA:pseudouridine synthase; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs; RIB2 has a paralog, PUS9, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0.06 0.05 0 0 0 – 0 0
Cytoplasm 0.95 0.98 0.9 0.98 0.98 0.94 0.9 0.91 0.74 0.75 0.61 0.7 – – – 0.96 0.97 0.98 0.88 0.89 0.73 – 0.75 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0.12 0
Mitochondria 0 0 0.05 0 0 0.1 0.1 0.09 0.35 0.22 0.52 0.39 – – – 0 0 0 0 0 0.11 – 0 0.07
Nucleus 0 0 0.08 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 2 0 1 0 0 – – – 0 0 0 0 1 0 – 0 0
Bud 1 1 0 1 0 0 2 2 4 2 5 4 – – – 0 0 0 1 8 0 – 0 0
Bud Neck 0 0 0 2 1 0 0 6 3 1 0 1 – – – 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 4 0 2 4 6 3 4 2 5 2 2 – – – 6 18 14 0 0 0 – 0 0
Cytoplasm 142 299 35 113 183 164 209 328 116 126 117 124 – – – 299 311 294 132 267 49 – 5 12
Endoplasmic Reticulum 2 4 0 0 0 1 2 2 1 4 2 0 – – – 12 11 9 1 1 1 – 0 0
Endosome 0 0 0 0 0 0 0 0 1 1 0 4 – – – 1 0 1 3 2 1 – 0 0
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 – – – 0 1 0 0 2 0 – 0 0
Mitochondria 6 1 2 0 1 18 22 32 54 37 100 69 – – – 7 4 1 4 4 7 – 0 1
Nucleus 0 1 3 0 1 1 0 5 3 4 0 0 – – – 2 5 4 0 0 1 – 0 0
Nuclear Periphery 0 2 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 – – – 1 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 1 1 0 0 1 1 – – – 1 0 0 1 3 0 – 0 0
Vac/Vac Membrane 0 2 0 1 2 1 4 4 4 7 4 7 – – – 8 4 2 0 3 0 – 0 0
Unique Cell Count 150 306 39 115 186 174 231 362 156 168 192 178 312 320 301 150 299 68 8 16
Labelled Cell Count 152 315 40 119 192 192 244 386 188 188 231 212 337 354 325 150 299 68 8 16


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 6.6 5.8 6.5 6.8 5.6 5.7 5.6 4.6 4.8 4.1 5.1 – – – 7.9 8.0 7.7 – – –
Std Deviation (1e-4) 1.5 2.1 1.5 1.7 2.0 2.2 1.8 2.0 1.8 1.6 1.1 2.1 – – – 1.5 1.4 1.3 – – –
Intensity Change (Log2) – – – 0.16 0.23 -0.05 -0.03 -0.05 -0.33 -0.28 -0.49 -0.19 – – – 0.44 0.47 0.41 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cytoplasm 2.4 2.8 1.0 0.1 0.2 -2.1 -2.0 -3.5 -2.6 – – – 1.7 2.3 2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 4.1 – – – 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4992 2.0553 2.0177 1.6238 1.7217 1.7074 0.6987 1.2627 1.105 1.1125 0.3571 1.0582 -0.1951 1.1734 0.221 -0.2205 1.0574 0.7908
Actin 0.0273 0.0017 0.0044 0.0008 0.0406 0.0015 0.0202 0.0006 0.0088 0.0003 0.0007 0.0081 0.023 0.0007 0.0048 0.0005 0.0614 0.0158
Bud 0.0121 0.0084 0.0029 0.0034 0.0016 0.0031 0.0004 0.0002 0.0012 0.0005 0.0012 0.0005 0.0002 0.0038 0.0002 0.0001 0.0015 0.0003
Bud Neck 0.0021 0.0003 0.0006 0.0048 0.0011 0.0005 0.0006 0.0002 0.002 0.0003 0.0005 0.0006 0.0008 0.0002 0.0013 0.0001 0.0052 0.0005
Bud Periphery 0.006 0.0013 0.0012 0.0025 0.0028 0.0018 0.0006 0.0002 0.0027 0.001 0.0069 0.0005 0.0002 0.0031 0.0001 0 0.0019 0.0005
Bud Site 0.0144 0.0081 0.0022 0.0021 0.0019 0.0002 0.0026 0.0043 0.0073 0.0003 0.0011 0.0002 0.0033 0.0079 0.0011 0.0001 0.0073 0.0016
Cell Periphery 0.0007 0.0003 0.0002 0.0002 0.0001 0.0001 0.0001 0.0003 0.0003 0.0001 0.0005 0.0001 0.0002 0.0002 0.0001 0.0001 0.0005 0.0002
Cytoplasm 0.4227 0.7052 0.6655 0.4483 0.5308 0.707 0.3735 0.5227 0.4559 0.5659 0.3406 0.5845 0.4533 0.5759 0.6062 0.4897 0.4562 0.7138
Cytoplasmic Foci 0.0412 0.0088 0.0118 0.0506 0.0513 0.0142 0.0466 0.0051 0.015 0.0092 0.011 0.0078 0.028 0.0061 0.0112 0.006 0.0517 0.0035
Eisosomes 0.0004 0.0001 0.0001 0.0001 0.0002 0 0.0003 0.0001 0.0001 0 0.0002 0.0002 0.0003 0 0.0001 0 0.0013 0.0002
Endoplasmic Reticulum 0.0043 0.0017 0.0024 0.0022 0.003 0.0021 0.0022 0.001 0.0025 0.0022 0.002 0.0015 0.0039 0.0022 0.0031 0.0073 0.0035 0.001
Endosome 0.0199 0.0035 0.0106 0.0088 0.0486 0.0064 0.0295 0.0009 0.0089 0.0405 0.0248 0.0025 0.0125 0.0036 0.0043 0.0046 0.073 0.0015
Golgi 0.0063 0.0003 0.0032 0.002 0.0167 0.0007 0.0082 0.0002 0.0046 0.0051 0.0018 0.0012 0.0023 0.0004 0.0034 0.0003 0.0514 0.0007
Lipid Particles 0.017 0.0001 0.0033 0.0015 0.0124 0.0008 0.015 0.0001 0.0103 0.0004 0.0277 0.0007 0.0031 0.0003 0.0077 0.0002 0.0389 0.0009
Mitochondria 0.0221 0.0005 0.015 0.0057 0.0126 0.0008 0.0058 0.0002 0.0046 0.0072 0.0099 0.002 0.0008 0.0148 0.0008 0.0001 0.036 0.0008
None 0.3414 0.255 0.2648 0.4472 0.2208 0.256 0.4473 0.4615 0.4594 0.3429 0.5365 0.3842 0.4579 0.3778 0.3336 0.4879 0.1364 0.233
Nuclear Periphery 0.0106 0.0005 0.0011 0.001 0.0046 0.0009 0.0064 0.0003 0.0025 0.0008 0.0111 0.0008 0.0013 0.0004 0.0007 0.0008 0.0061 0.0015
Nucleolus 0.0022 0.0001 0.0009 0.001 0.0008 0.0002 0.0007 0.0001 0.0002 0.0001 0.0035 0.0001 0.0005 0.0001 0.0001 0 0.0006 0.0006
Nucleus 0.0044 0.0018 0.0036 0.0027 0.0048 0.0012 0.0018 0.0009 0.0018 0.0013 0.0028 0.0009 0.0011 0.0005 0.0007 0.0005 0.0016 0.02
Peroxisomes 0.0285 0.0002 0.0014 0.0108 0.0193 0.0004 0.0198 0.0002 0.0084 0.0002 0.0043 0.0008 0.0022 0.0006 0.0188 0 0.0547 0.002
Punctate Nuclear 0.0109 0.0006 0.001 0.0021 0.0233 0.0007 0.0158 0.0003 0.0013 0.0003 0.0067 0.0024 0.003 0.0004 0.0005 0.0003 0.0045 0.0004
Vacuole 0.0042 0.0012 0.0025 0.0017 0.0019 0.0011 0.0019 0.0005 0.0015 0.0131 0.0047 0.0004 0.0018 0.001 0.0011 0.0011 0.0036 0.0009
Vacuole Periphery 0.0014 0.0002 0.0011 0.0004 0.001 0.0002 0.0008 0 0.0004 0.0082 0.0016 0.0002 0.0003 0.0001 0.0002 0.0002 0.0027 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.8428 11.1078 11.5395 13.5636 13.6939 10.556 14.1793 12.1284 11.6726 15.3923
Translational Efficiency 0.9585 1.057 1.1293 0.9493 0.9271 1.1737 0.7952 0.9917 1.0511 0.9033

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Bifunctional DRAP deaminase tRNA:pseudouridine synthase; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs; RIB2 has a paralog, PUS9, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (62%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rib2

Rib2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rib2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available