Standard name
Human Ortholog
Description Cargo-binding mu subunit of AP-2; AP-2 is a heterotetrameric endocytic cargo-binding adaptor that facilitates uptake of membrane proteins during clathrin-mediated endocytosis; Apm4p is required for AP-2 function and localization, and binds cell wall stress receptor Mid2p; AP-2 is required for cell polarity responses to pheromone, nutritional status and cell wall damage in S. cerevisiae, and for hyphal growth in C. albicans; AP-2 complex is conserved in mammals

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.46 0.59 0.43 0.34 0.23 0.14 0.15 0.14 0.12 0.13 0.13 0.52 0.42 0.33 0.35 0.45 0.42 0.43 0.41 0.52 0.45 0.4 0.35
Bud 0.14 0.2 0.12 0.18 0.16 0.14 0.13 0.12 0.13 0.12 0.12 0.1 0.06 0.07 0.12 0.11 0.11 0.07 0.08 0.05 0.05 0.05 0
Bud Neck 0.08 0 0.09 0 0.05 0 0 0 0 0 0 0.14 0.12 0.17 0.19 0.15 0.06 0.29 0.27 0.2 0.22 0.16 0.19
Bud Site 0.2 0.11 0.16 0.18 0.14 0.13 0 0.06 0.07 0.07 0.06 0 0.05 0 0.24 0.13 0.18
Cell Periphery 0.13 0.12 0.12 0.11 0.06 0.1 0.1 0.12 0.11 0.16 0.11 0.13 0.08 0.08 0.1 0.07 0.12 0.07 0.1 0.14 0.11 0.2 0.17
Cytoplasm 0.17 0.22 0.16 0.12 0.14 0.18 0.18 0.14 0.12 0.13 0.1 0.3 0.31 0.41 0.21 0.26 0.21 0 0 0 0 0 0.06
Endoplasmic Reticulum 0.07 0.07 0.06 0.07 0.1 0.1 0.1 0.07 0 0.06 0.05 0 0.08 0.05 0 0 0 0 0 0 0 0 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.15 0.07 0.22 0.16 0.4 0.5 0.68 0.69 0.73 0.72 0 0 0 0.11 0.09 0.1 0 0 0 0.07 0.07 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.07 0 0.13 0.32 0.27 0.2 0.14 0.09 0.12 0.09 0.11 0 0 0 0.05 0 0.08 0 0 0 0 0 0
SpindlePole 0.06 0 0.13 0.08 0.08 0 0 0 0 0 0 0.15 0.17 0.18 0.21 0.17 0.18 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 104 114 147 90 63 64 90 74 73 62 81 112 110 69 45 67 62 138 104 247 128 249 197
Bud 32 38 42 48 44 62 80 63 78 57 71 21 15 15 16 17 16 21 20 21 15 28 21
Bud Neck 17 6 31 8 14 4 3 3 1 4 2 29 32 36 25 23 9 95 67 97 60 100 109
Bud Site 45 22 54 48 38 57 25 31 45 35 37 7 12 5 31 20 26
Cell Periphery 30 23 41 29 16 44 58 62 71 76 68 28 21 16 13 11 17 24 24 65 30 125 96
Cytoplasm 38 42 56 33 37 80 107 70 73 61 60 65 80 86 27 39 30 3 5 5 5 24 35
Endoplasmic Reticulum 16 13 21 18 26 45 61 37 26 26 32 8 21 11 4 4 4 3 4 6 5 22 32
Endosome 1 1 8 12 5 7 12 2 6 2 3 1 1 0 0 1 0 8 5 8 4 3 8
Golgi 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 1 0 4 1
Mitochondria 24 30 23 59 44 180 295 350 428 343 441 1 0 0 14 13 14 12 9 12 19 42 32
Nucleus 6 1 6 3 3 6 2 4 8 2 6 3 6 6 10 4 9 0 0 0 0 0 0
Nuclear Periphery 1 1 0 0 2 0 3 2 4 3 6 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 1 0 1 2 10 6 3 5 10 0 0 0 0 1 1 0 0 0 0 0 0
Peroxisomes 16 2 46 87 74 88 86 47 75 41 70 0 1 0 6 1 12 1 0 4 1 1 1
SpindlePole 13 3 45 22 22 11 20 6 7 8 13 32 45 37 27 25 26 1 0 0 0 0 0
Vac/Vac Membrane 1 0 1 2 7 6 13 4 10 5 9 0 0 0 0 1 3 5 5 2 5 10 17
Unique Cell Count 224 194 342 268 270 447 595 512 622 472 610 214 259 208 130 150 146 325 255 476 283 620 563
Labelled Cell Count 344 298 522 459 396 656 865 761 908 730 909 307 344 281 218 227 229 325 255 476 283 620 563


Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.6 5.2 5.1 5.1 4.5 4.8 4.4 4.3 4.3 4.5 6.3 6.5 6.6 5.2 5.7 6.7 6.2 6.7 6.8
Std Deviation (1e-4) 1.0 1.0 1.2 1.5 1.7 1.3 1.1 1.0 1.2 1.0 1.2 0.8 0.9 0.9 1.1 1.3 1.5 1.2 1.7 1.5
Intensity Change (Log2) -0.03 -0.04 -0.21 -0.14 -0.25 -0.27 -0.27 -0.23 0.26 0.32 0.33 -0.01 0.13 0.35 0.25 0.36 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.4 -5.1 0 0 0 0 0 0 2.2 -0.1 -2.3 -1.7 0.3 -0.1
Bud 1.9 1.4 0 0 0 0 0 0 -0.9 -2.7 -1.9 0 -0.3 -0.4
Bud Neck -3.0 -1.8 0 0 0 0 0 0 1.7 1.3 2.9 3.1 2.0 -1.1
Bud Site 0.7 -0.6 0 0 0 0 0 0 -4.6 -4.3 -4.9 2.0 -0.7 0.6
Cell Periphery -0.4 -2.6 0 0 0 0 0 0 0.4 -1.5 -1.6 -0.6 -1.5 -0.1
Cytoplasm -1.4 -0.9 0.6 0.6 -1.1 -2.0 -1.4 -3.0 3.9 4.2 6.5 1.1 2.5 1.1
Endoplasmic Reticulum 0.3 1.6 0 0 0 0 0 0 -1.2 0.9 -0.4 -1.3 -1.6 -1.6
Endosome 1.5 -0.4 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.5 3.8 0 0 0 0 0 0 -3.5 -4.3 -3.8 1.5 0.8 1.1
Nucleus 0 0 -0.5 0 0 0 0 0 0 0.5 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 5.6 4.3 0 0 0 0 0 0 -5.6 -5.9 -5.5 -2.7 -4.4 -1.6
SpindlePole -1.9 -2.0 0 0 0 0 0 0 0.6 1.4 1.5 2.1 1.0 1.3
Vacuole 0 0 0 2.3 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7116 1.7452 2.1669 1.8294 2.2888 2.0394 3.8472 3.615 3.8094 3.9216 3.7153 3.9273 3.4374 3.5754 3.4758 3.6086 3.6568 3.6337
Actin 0.1316 0.0814 0.0529 0.0109 0.1626 0.1095 0.1475 0.0664 0.0588 0.0435 0.0692 0.1089 0.1653 0.1109 0.0836 0.0213 0.1223 0.121
Bud 0.0061 0.0065 0.0048 0.0006 0.0171 0.0044 0.0033 0.0066 0.0024 0.0029 0.0024 0.0039 0.0035 0.006 0.002 0.0016 0.0016 0.0025
Bud Neck 0.1214 0.0244 0.5007 0.9105 0.4092 0.5292 0.1244 0.0586 0.6114 0.7397 0.6749 0.6145 0.18 0.0513 0.6388 0.7849 0.7462 0.5983
Bud Periphery 0.0273 0.0185 0.0211 0.0026 0.0419 0.0286 0.0139 0.0149 0.0071 0.0123 0.0119 0.0307 0.0179 0.0162 0.0067 0.006 0.0057 0.0163
Bud Site 0.2075 0.5282 0.2287 0.0128 0.0385 0.0265 0.2869 0.6044 0.2347 0.0534 0.0437 0.0188 0.3511 0.5764 0.2011 0.0776 0.0301 0.0267
Cell Periphery 0.0793 0.0401 0.021 0.0041 0.0093 0.0313 0.1023 0.0315 0.0097 0.0156 0.022 0.0416 0.0877 0.0341 0.015 0.0104 0.0234 0.0451
Cytoplasm 0.1433 0.1753 0.0517 0.0061 0.0181 0.1195 0.1275 0.0907 0.0175 0.0254 0.0393 0.1024 0.0948 0.1288 0.0157 0.0315 0.0404 0.1397
Cytoplasmic Foci 0.0321 0.0082 0.0054 0.0161 0.027 0.0175 0.0327 0.0034 0.0069 0.0076 0.0129 0.0044 0.0109 0.0067 0.0045 0.0132 0.0039 0.0022
Eisosomes 0.005 0.0051 0.001 0.0001 0.0013 0.0025 0.0084 0.0037 0.0009 0.0028 0.0015 0.002 0.0091 0.0022 0.001 0.002 0.0011 0.0016
Endoplasmic Reticulum 0.0096 0.0062 0.0026 0.0007 0.0053 0.0088 0.0055 0.0026 0.0015 0.0006 0.0014 0.0015 0.0024 0.002 0.0007 0.0021 0.0011 0.0006
Endosome 0.0355 0.0021 0.0077 0.0126 0.0294 0.0106 0.0182 0.0006 0.0012 0.0017 0.0293 0.0029 0.0066 0.0035 0.0043 0.0045 0.0045 0.0002
Golgi 0.0173 0.0033 0.0036 0.0086 0.0284 0.0057 0.0119 0.0012 0.0012 0.0017 0.0125 0.0036 0.0051 0.005 0.0027 0.0024 0.0046 0.0008
Lipid Particles 0.0213 0.0025 0.0082 0.0057 0.0366 0.0046 0.0201 0.0007 0.0009 0.0053 0.0014 0.0012 0.0029 0.0011 0.0029 0.0043 0.0026 0.0002
Mitochondria 0.0106 0.0007 0.0053 0.0011 0.0333 0.0211 0.0181 0.0007 0.0003 0.0029 0.0114 0.0025 0.0013 0.0005 0.0007 0.0013 0.0004 0.0002
None 0.0705 0.0898 0.0679 0.0004 0.0472 0.0557 0.0438 0.1032 0.0342 0.0526 0.0544 0.0581 0.0488 0.051 0.0094 0.0165 0.0069 0.043
Nuclear Periphery 0.0212 0.0006 0.0015 0.0003 0.0058 0.0067 0.0035 0.0011 0.0014 0.0001 0.0006 0.0003 0.0021 0.0002 0.0006 0.0003 0.0014 0.0001
Nucleolus 0.0022 0.0006 0.0009 0.0003 0.0054 0.0009 0.0013 0.001 0.0003 0.0009 0.0007 0.0002 0.0007 0.0006 0.0025 0.0013 0.0003 0.0002
Nucleus 0.0171 0.0012 0.0013 0.0004 0.0085 0.002 0.0016 0.002 0.0024 0.0007 0.0008 0.0005 0.001 0.0008 0.0018 0.0011 0.0004 0.0004
Peroxisomes 0.0148 0.0021 0.0081 0.0047 0.0538 0.0078 0.0222 0.0018 0.0058 0.0285 0.0068 0.0007 0.0025 0.001 0.0017 0.0116 0.001 0.0003
Punctate Nuclear 0.0145 0.0009 0.0007 0.0007 0.0072 0.0025 0.0026 0.001 0.0005 0.0005 0.0009 0.0002 0.0009 0.0003 0.0014 0.0034 0.0015 0.0001
Vacuole 0.0101 0.0021 0.0042 0.0007 0.0111 0.0035 0.0037 0.0038 0.0009 0.0013 0.0014 0.0009 0.0048 0.0013 0.0027 0.0024 0.0004 0.0007
Vacuole Periphery 0.0016 0.0001 0.0007 0.0001 0.0031 0.001 0.0009 0.0001 0.0001 0.0001 0.0005 0.0002 0.0003 0.0001 0.0002 0.0002 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.6404 18.0435 29.2575 34.0511 28.5929 25.4031 31.7576 35.1157 33.0894 33.9416
Translational Efficiency 0.4757 0.9508 0.5344 0.4807 0.3895 0.5761 0.6611 0.534 0.4989 0.3329

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
107 1152 817 1315 2210 1511 210 1293 2317 2663 1027 2608

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 910.32 765.74 910.19 912.76 721.25 786.54 1025.25 916.91 729.98 777.54 933.72 914.82
Standard Deviation 90.74 113.16 115.81 119.30 84.94 98.35 97.10 114.76 94.00 105.52 121.45 117.09
Intensity Change Log 2 -0.249519 -0.000206 0.003862 0.125021 0.507404 0.346281 -0.071872 0.246401 0.165323

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.027464 0.058961 0.113862* 0.162343* 0.047873 0.071152 0.079812 0.117657* 0.046930 0.065878 0.106899* 0.140188*
Bud Neck 0.208466* 0.237345* 0.144495 0.174069 0.393588* 0.329165* 0.241608* 0.222384* 0.385039* 0.289444* 0.164353 0.198023*
Bud Site 0.316951* 0.285173* 0.446683* 0.457808* 0.178302* 0.227636* 0.498979* 0.485942* 0.184705* 0.252526* 0.457376* 0.471756*
Cell Periphery 0.015582 0.022009 0.008841 0.062551 0.051326 0.033866 0.033593 0.050657 0.049676 0.028737 0.013902 0.056654
Cytoplasm 0.104165* 0.137023* 0.083689 0.058739 0.189432* 0.158458* 0.026704 0.060815 0.185495* 0.149186* 0.072037 0.059768
Cytoplasmic Foci 0.064370 0.076118 0.042434 0.005062 0.026775 0.040321 0.018999 0.005053 0.028511 0.055807 0.037642 0.005058
Eisosomes 0.002770 0.010774 0.002830 0.016191 0.030393 0.017084 0.004577 0.008768 0.029117 0.014355 0.003187 0.012511
Endoplasmic Reticulum 0.004194 0.003935 0.019888 0.005091 0.002381 0.001745 0.008889 0.003030 0.002465 0.002692 0.017639 0.004069
Endosome 0.021731 0.035894 0.018071 0.001884 0.005732 0.016763 0.005968 0.001620 0.006470 0.025039 0.015596 0.001753
Golgi 0.015526 0.026050 0.006454 0.001104 0.008355 0.014551 0.001474 0.001007 0.008686 0.019525 0.005436 0.001056
Lipid Particles 0.005157 0.014525 0.008577 0.001298 0.007145 0.007413 0.003689 0.000709 0.007054 0.010490 0.007578 0.001006
Mitochondria 0.000790 0.020529 0.001401 0.003036 0.020862 0.023944 0.000327 0.003268 0.019935 0.022467 0.001181 0.003151
Mitotic Spindle 0.007103 0.002778 0.003000 0.001652 0.000185 0.000732 0.000790 0.001133 0.000505 0.001617 0.002548 0.001394
None 0.000114 0.001020 0.000233 0.000055 0.000476 0.000455 0.000035 0.000924 0.000460 0.000699 0.000192 0.000486
Nuclear Periphery 0.000135 0.000077 0.000106 0.000061 0.000034 0.000039 0.000072 0.000023 0.000039 0.000055 0.000099 0.000042
Nuclear Periphery Foci 0.001371 0.000357 0.003933 0.000677 0.000144 0.000132 0.002152 0.000213 0.000201 0.000229 0.003569 0.000447
Nucleolus 0.000199 0.000210 0.001189 0.000018 0.000130 0.000585 0.000891 0.000022 0.000133 0.000423 0.001128 0.000020
Nucleus 0.040693 0.007984 0.024306 0.009152 0.009415 0.007767 0.011414 0.008662 0.010859 0.007860 0.021670 0.008909
Peroxisomes 0.016093 0.037397 0.019668 0.004257 0.019524 0.030446 0.012431 0.003804 0.019366 0.033453 0.018188 0.004032
Vacuole 0.146196 0.018874 0.049685 0.034458 0.007266 0.016221 0.047513 0.024186 0.013682 0.017369 0.049241 0.029365
Vacuole Periphery 0.000928 0.002967 0.000656 0.000495 0.000660 0.001526 0.000083 0.000123 0.000673 0.002149 0.000539 0.000310

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.38 -7.47 -12.84 -13.96 -5.53 -4.96 -3.00 -11.44 -6.74 -3.46 -5.05 -8.75 -19.43 -14.58 -4.97
Bud Neck -1.09 2.02 1.01 6.36 -3.36 6.04 7.78 17.33 10.00 0.73 10.61 23.20 22.75 12.34 -4.31
Bud Site 0.67 -3.41 -4.05 -14.29 -1.55 -6.42 -13.66 -30.92* -23.62 0.20 -10.01 -24.52 -37.20 -27.18 -2.13
Cell Periphery -1.59 1.75 -9.30 -10.36 -15.92 5.52 3.80 0.32 -4.37 -3.30 7.95 15.11 -2.01 -10.07 -17.23
Cytoplasm -1.72 1.20 2.64 11.06 3.76 4.31 22.53 20.80 14.72 -5.42 5.94 17.35 23.08 18.48 2.28
Cytoplasmic Foci -0.83 1.90 5.21 17.53 12.57 -4.80 1.50 12.55 13.85 3.55 -10.12 -3.46 14.03 22.09 13.07
Eisosomes -5.41 0.01 -8.71 -2.76 -9.40 6.78 15.07 13.14 5.61 -3.68 8.73 17.83 10.40 1.64 -10.84
Endoplasmic Reticulum 0.18 -6.59 -0.70 -1.60 7.23 1.45 -3.12 -1.06 -2.17 2.73 -0.48 -9.10 -3.23 -3.00 8.07
Endosome -2.39 0.66 3.64 13.58 9.22 -8.41 -0.32 8.31 11.88 2.80 -13.45 -6.11 9.08 17.69 9.69
Golgi -1.31 1.08 1.72 12.56 7.50 -4.29 9.52 9.86 9.88 0.83 -8.18 4.00 10.14 15.89 7.72
Lipid Particles -4.23 -1.86 3.00 7.10 4.97 -0.29 2.72 10.14 8.03 3.48 -3.19 -0.62 9.55 10.07 5.73
Mitochondria -10.59 -1.62 -2.31 8.51 -1.69 -1.34 16.26 10.70 9.40 -2.72 -1.39 15.37 12.07 12.69 -2.67
Mitotic Spindle 0.70 0.56 0.88 1.14 2.12 -3.19 -2.01 -2.40 -0.91 -0.68 -2.44 -3.85 -2.05 0.44 2.41
None -2.45 -1.74 1.14 2.62 2.65 0.08 5.10 -0.58 -0.59 -1.16 -1.20 2.21 -0.08 0.50 -0.67
Nuclear Periphery 1.74 -0.32 1.64 0.31 2.24 -0.55 -3.13 1.88 1.61 3.65 -1.63 -6.49 -0.31 0.53 4.07
Nuclear Periphery Foci 2.89 -3.59 1.54 -1.23 4.53 0.22 -3.69 -1.53 -2.95 3.53 -0.39 -5.91 -1.76 -1.70 5.41
Nucleolus -0.16 -3.43 3.45 3.45 3.82 -1.29 -0.97 4.13 1.58 1.06 -1.43 -3.55 4.57 1.97 3.80
Nucleus 3.31 0.92 3.13 -1.49 6.28 1.89 -1.10 -0.01 -1.28 1.07 3.51 -5.95 1.29 -1.99 6.37
Peroxisomes -2.62 -0.44 1.47 13.08 8.25 -4.88 2.23 11.86 13.45 2.94 -7.31 0.67 12.12 18.74 8.91
Vacuole 5.75 3.96 4.79 -6.68 3.82 -7.26 -4.73 -8.35 -4.35 2.86 -2.08 -11.05 -8.62 -8.01 5.86
Vacuole Periphery -2.82 0.51 0.80 3.84 0.64 -2.98 9.85 8.51 4.90 -1.02 -4.87 1.30 2.33 5.55 1.56