Standard name | |
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Description | Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; primary component of the Chs5/6 complex that binds directly to membranes; CHS6 has a paralog, BCH2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 2 | 15 | 3 | 14 | 5 | 9 | 8 | 5 | 2 | 0 | 3 | 4 | 1 | 4 | 11 | 2 | – | 4 | 5 | 4 | 13 | 29 | 44 |
Bud | 4 | 4 | 2 | 6 | 6 | 11 | 26 | 25 | 9 | 14 | 11 | 18 | 14 | 8 | 8 | 0 | 0 | – | 0 | 0 | 0 | 2 | 3 | 2 |
Bud Neck | 0 | 0 | 3 | 1 | 4 | 1 | 7 | 9 | 1 | 0 | 0 | 6 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 2 | 1 |
Bud Site | 1 | 0 | 0 | 0 | 1 | 1 | 7 | 4 | 3 | 4 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 23 | 28 | 44 | 47 | 38 | 37 | 44 | 74 | 17 | 25 | 31 | 51 | 60 | 121 | 140 | 26 | 7 | – | 7 | 9 | 14 | 3 | 6 | 4 |
Endoplasmic Reticulum | 8 | 8 | 11 | 2 | 12 | 7 | 2 | 1 | 8 | 1 | 5 | 0 | 15 | 5 | 11 | 3 | 1 | – | 0 | 1 | 0 | 1 | 3 | 11 |
Endosome | 17 | 3 | 4 | 14 | 18 | 28 | 13 | 33 | 0 | 1 | 2 | 3 | 25 | 70 | 114 | 16 | 4 | – | 62 | 46 | 31 | 30 | 61 | 87 |
Golgi | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 5 | 1 | – | 29 | 18 | 7 | 23 | 84 | 118 |
Mitochondria | 101 | 104 | 30 | 58 | 42 | 167 | 181 | 162 | 140 | 163 | 178 | 157 | 108 | 103 | 76 | 5 | 4 | – | 44 | 51 | 107 | 80 | 140 | 125 |
Nucleus | 0 | 0 | 0 | 1 | 0 | 4 | 2 | 4 | 3 | 3 | 3 | 2 | 1 | 1 | 1 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 2 | 13 | 9 | 7 | 0 | 0 | 0 | 1 | 6 | 12 | 17 | 0 | 0 | – | 10 | 1 | 8 | 9 | 23 | 39 |
SpindlePole | 8 | 1 | 8 | 7 | 31 | 43 | 63 | 84 | 6 | 15 | 14 | 37 | 7 | 9 | 7 | 8 | 0 | – | 11 | 9 | 5 | 2 | 8 | 6 |
Vac/Vac Membrane | 3 | 2 | 2 | 2 | 3 | 7 | 5 | 12 | 2 | 2 | 2 | 1 | 6 | 19 | 22 | 9 | 2 | – | 4 | 7 | 8 | 7 | 8 | 9 |
Unique Cell Count | 142 | 130 | 106 | 125 | 147 | 250 | 277 | 339 | 158 | 189 | 211 | 225 | 208 | 306 | 342 | 68 | 16 | 178 | 154 | 195 | 178 | 378 | 459 | |
Labelled Cell Count | 177 | 155 | 121 | 146 | 171 | 324 | 369 | 423 | 194 | 234 | 251 | 282 | 250 | 351 | 403 | 86 | 21 | 178 | 154 | 195 | 178 | 378 | 459 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 4.4 | 5.2 | 5.0 | 5.3 | 4.3 | 4.4 | 4.5 | 3.7 | 3.6 | 3.6 | 4.0 | 5.1 | 5.1 | 5.4 | 8.5 | 7.4 | – | 4.9 | 5.0 | 5.6 |
Std Deviation (1e-4) | 0.5 | 0.7 | 0.8 | 1.2 | 1.0 | 0.9 | 1.2 | 0.9 | 0.6 | 0.7 | 0.8 | 0.9 | 0.8 | 1.0 | 1.0 | 1.5 | 0.9 | – | 1.0 | 1.5 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.05 | -0.26 | -0.22 | -0.2 | -0.5 | -0.53 | -0.53 | -0.38 | -0.03 | -0.03 | 0.06 | 0.72 | 0.51 | – | -0.09 | -0.04 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.0699 | 0.14 | -0.0945 | 0.0431 | 0.0801 | 0.2328 | 0.1471 | 0.5376 | 0.234 | 0.3435 | -0.1945 | 0.3552 | 0.2115 | 0.4451 | 0.1365 | 0.056 | 0.0604 | 0.2655 |
Actin | 0.0544 | 0.0098 | 0.0133 | 0.0601 | 0.0015 | 0.018 | 0.0481 | 0.0128 | 0.0055 | 0.0047 | 0.0864 | 0.0143 | 0.0701 | 0.0156 | 0.0215 | 0.0011 | 0.0009 | 0.0023 |
Bud | 0.0008 | 0.0019 | 0.0006 | 0.0009 | 0.0001 | 0.0006 | 0.0008 | 0.0006 | 0.0007 | 0.0006 | 0.0008 | 0.0008 | 0.0007 | 0.0009 | 0.0011 | 0.0006 | 0.0006 | 0.0002 |
Bud Neck | 0.0022 | 0.0009 | 0.0021 | 0.0009 | 0.0003 | 0.0051 | 0.0011 | 0.0004 | 0.0008 | 0.0006 | 0.0027 | 0.002 | 0.0029 | 0.0009 | 0.0017 | 0.0007 | 0.0003 | 0.0012 |
Bud Periphery | 0.0011 | 0.0026 | 0.0008 | 0.0014 | 0.0001 | 0.001 | 0.001 | 0.0004 | 0.0006 | 0.0004 | 0.0009 | 0.001 | 0.0011 | 0.0005 | 0.0008 | 0.0006 | 0.0006 | 0.0001 |
Bud Site | 0.0033 | 0.0174 | 0.0044 | 0.0055 | 0.0004 | 0.0008 | 0.0041 | 0.0033 | 0.0131 | 0.001 | 0.0012 | 0.0006 | 0.0039 | 0.0173 | 0.0046 | 0.0006 | 0.001 | 0.0003 |
Cell Periphery | 0.0005 | 0.0006 | 0.0003 | 0.0006 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.0691 | 0.1541 | 0.0998 | 0.0903 | 0.085 | 0.0617 | 0.0611 | 0.112 | 0.1341 | 0.0251 | 0.0236 | 0.0721 | 0.0502 | 0.0987 | 0.1116 | 0.0455 | 0.019 | 0.0465 |
Cytoplasmic Foci | 0.231 | 0.2472 | 0.2357 | 0.1955 | 0.2134 | 0.2067 | 0.2584 | 0.2939 | 0.2577 | 0.1908 | 0.1967 | 0.275 | 0.2559 | 0.2824 | 0.2564 | 0.2723 | 0.286 | 0.2571 |
Eisosomes | 0.001 | 0.0002 | 0.0003 | 0.0015 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.008 | 0.0054 | 0.0081 | 0.0058 | 0.0016 | 0.0037 | 0.0041 | 0.0022 | 0.0045 | 0.0016 | 0.0012 | 0.0032 | 0.0067 | 0.0023 | 0.0033 | 0.0024 | 0.002 | 0.0015 |
Endosome | 0.3036 | 0.305 | 0.3213 | 0.3228 | 0.363 | 0.343 | 0.306 | 0.2976 | 0.3598 | 0.4906 | 0.5916 | 0.3146 | 0.3157 | 0.3215 | 0.3294 | 0.516 | 0.4203 | 0.3688 |
Golgi | 0.0734 | 0.0869 | 0.0835 | 0.0682 | 0.1164 | 0.116 | 0.0999 | 0.1134 | 0.0919 | 0.1363 | 0.0632 | 0.1584 | 0.112 | 0.1255 | 0.1076 | 0.0981 | 0.1139 | 0.2016 |
Lipid Particles | 0.0538 | 0.0337 | 0.0346 | 0.0245 | 0.0549 | 0.0377 | 0.0648 | 0.0062 | 0.0169 | 0.0074 | 0.0059 | 0.0188 | 0.0462 | 0.0237 | 0.0117 | 0.0085 | 0.0199 | 0.0358 |
Mitochondria | 0.0194 | 0.0139 | 0.0247 | 0.0166 | 0.0114 | 0.0599 | 0.0233 | 0.0283 | 0.0098 | 0.0871 | 0.0069 | 0.0228 | 0.0136 | 0.0084 | 0.0176 | 0.014 | 0.0103 | 0.0115 |
None | 0.0844 | 0.0688 | 0.1147 | 0.1608 | 0.0735 | 0.0783 | 0.0641 | 0.0809 | 0.0583 | 0.0008 | 0.0006 | 0.0321 | 0.0537 | 0.0537 | 0.0879 | 0.0025 | 0.0496 | 0.0234 |
Nuclear Periphery | 0.0152 | 0.0022 | 0.0035 | 0.0118 | 0.0003 | 0.0016 | 0.0048 | 0.0009 | 0.0013 | 0.0004 | 0.0004 | 0.002 | 0.0054 | 0.0006 | 0.0013 | 0.0009 | 0.0006 | 0.0016 |
Nucleolus | 0.0015 | 0.0003 | 0.0006 | 0.0025 | 0.0001 | 0.0002 | 0.0016 | 0.0002 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0011 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0009 |
Nucleus | 0.0157 | 0.001 | 0.0016 | 0.0072 | 0.0001 | 0.0005 | 0.0084 | 0.0005 | 0.0007 | 0.0002 | 0.0001 | 0.001 | 0.0027 | 0.0005 | 0.0005 | 0.0002 | 0.0003 | 0.0014 |
Peroxisomes | 0.0474 | 0.0342 | 0.0317 | 0.0106 | 0.0728 | 0.0558 | 0.0352 | 0.0343 | 0.0297 | 0.0399 | 0.0105 | 0.0722 | 0.0351 | 0.0354 | 0.0332 | 0.0241 | 0.0529 | 0.0318 |
Punctate Nuclear | 0.0068 | 0.0019 | 0.0046 | 0.005 | 0.0008 | 0.0016 | 0.0034 | 0.001 | 0.0012 | 0.0004 | 0.0004 | 0.0007 | 0.0065 | 0.0008 | 0.0015 | 0.0006 | 0.0155 | 0.0093 |
Vacuole | 0.0055 | 0.009 | 0.011 | 0.0045 | 0.0027 | 0.0047 | 0.0064 | 0.0083 | 0.0099 | 0.007 | 0.0038 | 0.0052 | 0.0127 | 0.0087 | 0.0057 | 0.0068 | 0.0037 | 0.0029 |
Vacuole Periphery | 0.002 | 0.0028 | 0.0029 | 0.003 | 0.0015 | 0.0026 | 0.0029 | 0.0026 | 0.0029 | 0.0047 | 0.0028 | 0.0026 | 0.0028 | 0.002 | 0.0021 | 0.0044 | 0.0024 | 0.0016 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.8932 | 8.3629 | 10.4515 | 9.4663 | 8.6181 | 3.9368 | 12.6835 | 13.3664 | 11.2105 | 10.3661 |
Translational Efficiency | 0.8034 | 1.1879 | 0.6627 | 0.6944 | 0.5592 | 1.7073 | 0.7824 | 0.6162 | 0.6346 | 0.7128 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |