Standard name
Human Ortholog
Description Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; primary component of the Chs5/6 complex that binds directly to membranes; CHS6 has a paralog, BCH2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.14 0 0.1 0 0 0 0 0 0 0 0 0 0 0.16 0.12 0 0 0 0.08 0.08 0.1
Bud 0 0 0 0.05 0 0 0.09 0.07 0.06 0.07 0.05 0.08 0.07 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.16 0.22 0.42 0.38 0.26 0.15 0.16 0.22 0.11 0.13 0.15 0.23 0.29 0.4 0.41 0.38 0.44 0 0.06 0.08 0 0 0
Endoplasmic Reticulum 0.06 0.06 0.1 0 0.08 0 0 0 0.05 0 0 0 0.07 0 0 0 0.06 0 0 0 0 0 0
Endosome 0.12 0 0 0.11 0.12 0.11 0.05 0.1 0 0 0 0 0.12 0.23 0.33 0.24 0.25 0.35 0.3 0.16 0.17 0.16 0.19
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.16 0.12 0 0.13 0.22 0.26
Mitochondria 0.71 0.8 0.28 0.46 0.29 0.67 0.65 0.48 0.89 0.86 0.84 0.7 0.52 0.34 0.22 0.07 0.25 0.25 0.34 0.55 0.45 0.37 0.27
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0.06 0 0 0.05 0.06 0.09
SpindlePole 0.06 0 0.08 0.06 0.21 0.17 0.23 0.25 0 0.08 0.07 0.16 0 0 0 0.12 0 0.06 0.06 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.13 0.12 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 2 15 3 14 5 9 8 5 2 0 3 4 1 4 11 2 4 5 4 13 29 44
Bud 4 4 2 6 6 11 26 25 9 14 11 18 14 8 8 0 0 0 0 0 2 3 2
Bud Neck 0 0 3 1 4 1 7 9 1 0 0 6 0 0 0 0 0 0 0 1 0 2 1
Bud Site 1 0 0 0 1 1 7 4 3 4 4 3 0 0 0 0 0
Cell Periphery 0 0 1 2 0 0 0 0 0 1 0 0 0 1 0 3 0 0 0 0 0 0 0
Cytoplasm 23 28 44 47 38 37 44 74 17 25 31 51 60 121 140 26 7 7 9 14 3 6 4
Endoplasmic Reticulum 8 8 11 2 12 7 2 1 8 1 5 0 15 5 11 3 1 0 1 0 1 3 11
Endosome 17 3 4 14 18 28 13 33 0 1 2 3 25 70 114 16 4 62 46 31 30 61 87
Golgi 4 2 1 0 0 0 0 0 0 0 0 0 2 0 3 5 1 29 18 7 23 84 118
Mitochondria 101 104 30 58 42 167 181 162 140 163 178 157 108 103 76 5 4 44 51 107 80 140 125
Nucleus 0 0 0 1 0 4 2 4 3 3 3 2 1 1 1 0 0 0 0 1 0 0 0
Nuclear Periphery 1 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 2 0 0 1 0 0 1 0 0 2 1 0 2 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 2 13 9 7 0 0 0 1 6 12 17 0 0 10 1 8 9 23 39
SpindlePole 8 1 8 7 31 43 63 84 6 15 14 37 7 9 7 8 0 11 9 5 2 8 6
Vac/Vac Membrane 3 2 2 2 3 7 5 12 2 2 2 1 6 19 22 9 2 4 7 8 7 8 9
Unique Cell Count 142 130 106 125 147 250 277 339 158 189 211 225 208 306 342 68 16 178 154 195 178 378 459
Labelled Cell Count 177 155 121 146 171 324 369 423 194 234 251 282 250 351 403 86 21 178 154 195 178 378 459


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 4.4 5.2 5.0 5.3 4.3 4.4 4.5 3.7 3.6 3.6 4.0 5.1 5.1 5.4 8.5 7.4 4.9 5.0 5.6
Std Deviation (1e-4) 0.5 0.7 0.8 1.2 1.0 0.9 1.2 0.9 0.6 0.7 0.8 0.9 0.8 1.0 1.0 1.5 0.9 1.0 1.5 1.8
Intensity Change (Log2) -0.05 0.05 -0.26 -0.22 -0.2 -0.5 -0.53 -0.53 -0.38 -0.03 -0.03 0.06 0.72 0.51 -0.09 -0.04 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.0699 0.14 -0.0945 0.0431 0.0801 0.2328 0.1471 0.5376 0.234 0.3435 -0.1945 0.3552 0.2115 0.4451 0.1365 0.056 0.0604 0.2655
Actin 0.0544 0.0098 0.0133 0.0601 0.0015 0.018 0.0481 0.0128 0.0055 0.0047 0.0864 0.0143 0.0701 0.0156 0.0215 0.0011 0.0009 0.0023
Bud 0.0008 0.0019 0.0006 0.0009 0.0001 0.0006 0.0008 0.0006 0.0007 0.0006 0.0008 0.0008 0.0007 0.0009 0.0011 0.0006 0.0006 0.0002
Bud Neck 0.0022 0.0009 0.0021 0.0009 0.0003 0.0051 0.0011 0.0004 0.0008 0.0006 0.0027 0.002 0.0029 0.0009 0.0017 0.0007 0.0003 0.0012
Bud Periphery 0.0011 0.0026 0.0008 0.0014 0.0001 0.001 0.001 0.0004 0.0006 0.0004 0.0009 0.001 0.0011 0.0005 0.0008 0.0006 0.0006 0.0001
Bud Site 0.0033 0.0174 0.0044 0.0055 0.0004 0.0008 0.0041 0.0033 0.0131 0.001 0.0012 0.0006 0.0039 0.0173 0.0046 0.0006 0.001 0.0003
Cell Periphery 0.0005 0.0006 0.0003 0.0006 0 0.0003 0.0002 0.0001 0.0004 0.0001 0.0001 0.0002 0.0004 0.0003 0.0002 0.0001 0 0.0001
Cytoplasm 0.0691 0.1541 0.0998 0.0903 0.085 0.0617 0.0611 0.112 0.1341 0.0251 0.0236 0.0721 0.0502 0.0987 0.1116 0.0455 0.019 0.0465
Cytoplasmic Foci 0.231 0.2472 0.2357 0.1955 0.2134 0.2067 0.2584 0.2939 0.2577 0.1908 0.1967 0.275 0.2559 0.2824 0.2564 0.2723 0.286 0.2571
Eisosomes 0.001 0.0002 0.0003 0.0015 0.0001 0.0003 0.0003 0.0001 0 0 0.0001 0.0001 0.0007 0.0002 0.0002 0 0 0
Endoplasmic Reticulum 0.008 0.0054 0.0081 0.0058 0.0016 0.0037 0.0041 0.0022 0.0045 0.0016 0.0012 0.0032 0.0067 0.0023 0.0033 0.0024 0.002 0.0015
Endosome 0.3036 0.305 0.3213 0.3228 0.363 0.343 0.306 0.2976 0.3598 0.4906 0.5916 0.3146 0.3157 0.3215 0.3294 0.516 0.4203 0.3688
Golgi 0.0734 0.0869 0.0835 0.0682 0.1164 0.116 0.0999 0.1134 0.0919 0.1363 0.0632 0.1584 0.112 0.1255 0.1076 0.0981 0.1139 0.2016
Lipid Particles 0.0538 0.0337 0.0346 0.0245 0.0549 0.0377 0.0648 0.0062 0.0169 0.0074 0.0059 0.0188 0.0462 0.0237 0.0117 0.0085 0.0199 0.0358
Mitochondria 0.0194 0.0139 0.0247 0.0166 0.0114 0.0599 0.0233 0.0283 0.0098 0.0871 0.0069 0.0228 0.0136 0.0084 0.0176 0.014 0.0103 0.0115
None 0.0844 0.0688 0.1147 0.1608 0.0735 0.0783 0.0641 0.0809 0.0583 0.0008 0.0006 0.0321 0.0537 0.0537 0.0879 0.0025 0.0496 0.0234
Nuclear Periphery 0.0152 0.0022 0.0035 0.0118 0.0003 0.0016 0.0048 0.0009 0.0013 0.0004 0.0004 0.002 0.0054 0.0006 0.0013 0.0009 0.0006 0.0016
Nucleolus 0.0015 0.0003 0.0006 0.0025 0.0001 0.0002 0.0016 0.0002 0.0005 0.0001 0 0.0001 0.0011 0.0002 0.0002 0.0001 0.0002 0.0009
Nucleus 0.0157 0.001 0.0016 0.0072 0.0001 0.0005 0.0084 0.0005 0.0007 0.0002 0.0001 0.001 0.0027 0.0005 0.0005 0.0002 0.0003 0.0014
Peroxisomes 0.0474 0.0342 0.0317 0.0106 0.0728 0.0558 0.0352 0.0343 0.0297 0.0399 0.0105 0.0722 0.0351 0.0354 0.0332 0.0241 0.0529 0.0318
Punctate Nuclear 0.0068 0.0019 0.0046 0.005 0.0008 0.0016 0.0034 0.001 0.0012 0.0004 0.0004 0.0007 0.0065 0.0008 0.0015 0.0006 0.0155 0.0093
Vacuole 0.0055 0.009 0.011 0.0045 0.0027 0.0047 0.0064 0.0083 0.0099 0.007 0.0038 0.0052 0.0127 0.0087 0.0057 0.0068 0.0037 0.0029
Vacuole Periphery 0.002 0.0028 0.0029 0.003 0.0015 0.0026 0.0029 0.0026 0.0029 0.0047 0.0028 0.0026 0.0028 0.002 0.0021 0.0044 0.0024 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.8932 8.3629 10.4515 9.4663 8.6181 3.9368 12.6835 13.3664 11.2105 10.3661
Translational Efficiency 0.8034 1.1879 0.6627 0.6944 0.5592 1.7073 0.7824 0.6162 0.6346 0.7128

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1269 42 906 1206 1525 1698 2188 110 2794 1740 3094 1316

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 678.21 777.59 979.47 920.52 690.42 786.81 918.12 1342.52 684.87 786.59 936.08 955.79
Standard Deviation 75.90 101.35 125.04 114.71 85.91 106.71 104.74 211.61 81.74 106.59 114.52 171.59
Intensity Change Log 2 0.197278 0.530269 0.440717 0.188541 0.411208 0.959397 0.192877 0.471436 0.725530

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000674 0.000894 0.009118 0.011073 0.000693 0.001224 0.002236 0.002778 0.000684 0.001216 0.004252 0.010380
Bud Neck 0.013500 0.003866 0.009099 0.011685 0.006041 0.004377 0.016573 0.015798 0.009429 0.004365 0.014385 0.012029
Bud Site 0.006131 0.008717 0.033261 0.046134 0.006766 0.009696 0.015011 0.032338 0.006478 0.009673 0.020355 0.044981
Cell Periphery 0.000125 0.000085 0.000161 0.000102 0.000103 0.000086 0.000069 0.000030 0.000113 0.000086 0.000096 0.000096
Cytoplasm 0.103028* 0.059434 0.134374* 0.099218 0.043582 0.040850 0.166397* 0.162883* 0.070581 0.041298 0.157020* 0.104539*
Cytoplasmic Foci 0.514193* 0.413982* 0.231340* 0.302990* 0.411800* 0.396308* 0.255452* 0.226058* 0.458305* 0.396735* 0.248391* 0.296559*
Eisosomes 0.000329 0.000113 0.000080 0.000083 0.000213 0.000171 0.000081 0.000033 0.000265 0.000169 0.000081 0.000079
Endoplasmic Reticulum 0.000521 0.000545 0.009130 0.001910 0.000751 0.000554 0.002529 0.000140 0.000646 0.000554 0.004462 0.001762
Endosome 0.051703 0.177089 0.064639 0.108639 0.100407 0.122053 0.096207 0.109403 0.078286 0.123381 0.086963 0.108703
Golgi 0.121738* 0.160674* 0.021821 0.038421 0.259658* 0.268160* 0.013308 0.034772 0.197016* 0.265566* 0.015801 0.038116
Lipid Particles 0.035533 0.008145 0.022580 0.007362 0.026264 0.019677 0.024751 0.003140 0.030474 0.019398 0.024115 0.007009
Mitochondria 0.047786 0.035346 0.002913 0.005210 0.077180 0.059138 0.008656 0.013987 0.063830 0.058564 0.006974 0.005943
Mitotic Spindle 0.008264 0.009377 0.265723* 0.118007* 0.010130 0.015203 0.165809* 0.192580* 0.009283 0.015062 0.195066* 0.124240*
None 0.001988 0.000429 0.000687 0.000229 0.000879 0.000346 0.001126 0.000236 0.001383 0.000348 0.000998 0.000230
Nuclear Periphery 0.000235 0.000123 0.002794 0.000471 0.000272 0.000115 0.003325 0.000283 0.000255 0.000115 0.003170 0.000455
Nuclear Periphery Foci 0.000680 0.000285 0.013154 0.002296 0.000430 0.000299 0.005578 0.000231 0.000544 0.000298 0.007797 0.002124
Nucleolus 0.002599 0.015752 0.001168 0.000525 0.001898 0.000681 0.004906 0.000869 0.002217 0.001044 0.003812 0.000554
Nucleus 0.014851 0.010161 0.041171 0.006542 0.005160 0.001897 0.047325 0.009664 0.009561 0.002096 0.045523 0.006803
Peroxisomes 0.064188 0.065229 0.027053 0.082935 0.028767 0.037197 0.047945 0.075480 0.044855 0.037874 0.041828 0.082312
Vacuole 0.009145 0.018310 0.107914 0.151967 0.012290 0.015402 0.120569 0.117529 0.010862 0.015472 0.116863 0.149089
Vacuole Periphery 0.002790 0.011445 0.001819 0.004200 0.006715 0.006566 0.002146 0.001767 0.004932 0.006683 0.002051 0.003997

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.95 -7.31 -6.07 -5.82 0.04 -3.00 -5.30 -1.98 -1.32 -0.45 -3.18 -8.88 -6.17 -5.66 -3.29
Bud Neck 5.25 1.66 0.18 -6.27 -2.08 2.95 -14.73 -3.85 -4.31 -0.39 7.02 -8.42 -4.32 -11.37 3.07
Bud Site -0.73 -10.21 -13.98 -7.41 -2.43 -2.30 -6.47 -3.14 -2.62 -1.92 -2.80 -11.96 -14.41 -12.10 -8.32
Cell Periphery 1.40 -2.57 1.64 -0.15 5.04 1.22 2.94 6.10 7.33 9.71 2.20 1.35 1.78 0.16 1.12
Cytoplasm 2.09 -4.83 -3.27 -3.23 1.86 0.77 -23.97 -6.70 -6.82 -0.77 7.88 -19.80 -10.44 -15.61 5.08
Cytoplasmic Foci 2.90 34.34 30.75 4.46 -5.81 2.20 26.81 12.76 11.90 2.05 9.89 43.13 30.30 19.95 -4.07
Eisosomes 4.61 5.89 6.14 1.73 1.79 2.88 10.97 14.00 13.34 8.56 4.45 9.25 9.65 10.10 2.40
Endoplasmic Reticulum -0.14 -12.40 -3.64 -3.35 9.84 1.53 -11.83 5.56 4.56 18.49 0.89 -16.27 -3.18 -3.42 7.89
Endosome -3.41 -2.10 -7.68 2.49 -5.70 -4.43 3.75 1.30 2.86 0.14 -10.82 0.44 -1.61 7.88 -1.99
Golgi -1.09 20.97 18.62 3.78 -2.90 -1.05 41.54* 30.89 32.51 -2.77 -9.82 44.46 37.31 39.22 -6.04
Lipid Particles 11.10 5.26 12.87 0.14 7.79 2.95 2.19 12.80 9.92 13.58 5.67 5.35 15.95 7.47 12.32
Mitochondria 0.64 15.55 14.63 1.63 -1.24 3.79 18.88 14.95 12.28 -2.00 1.30 23.75 23.38 17.50 1.91
Mitotic Spindle -0.25 -22.55* -16.03 -14.75 10.33 -2.63 -26.25* -5.91 -5.71 -0.57 -3.24 -34.32 -17.06 -15.86 7.52
None 3.50 3.17 4.15 0.67 2.95 2.17 -0.70 2.25 0.25 7.27 4.43 1.66 4.60 0.40 7.60
Nuclear Periphery 1.26 -8.96 -5.82 -7.35 7.34 1.45 -14.66 -2.15 -5.34 14.09 2.02 -17.51 -5.79 -9.15 14.63
Nuclear Periphery Foci 1.78 -10.45 -5.46 -7.18 8.41 1.64 -11.36 0.86 -0.67 11.53 2.19 -15.11 -6.27 -7.18 9.29
Nucleolus -0.87 2.12 2.83 0.98 2.29 2.10 -5.80 0.69 -2.27 8.50 2.00 -4.85 3.03 0.29 11.10
Nucleus 0.63 -8.71 1.85 -0.16 9.69 3.63 -19.44 -2.63 -3.42 7.53 8.80 -20.13 -1.91 -9.06 18.08
Peroxisomes -0.07 11.42 -0.30 -0.02 -10.35 -3.42 -5.01 -2.88 -2.13 -1.78 2.70 3.84 -5.58 -7.05 -7.90
Vacuole -1.12 -21.50 -30.65 -19.12 -8.64 -2.24 -34.33 -9.07 -8.89 -1.44 -3.74 -40.80 -31.69 -30.68 -9.76
Vacuole Periphery -1.68 4.15 -0.34 1.64 -2.41 0.23 10.38 10.43 10.79 1.51 -3.41 10.98 3.77 5.94 -2.26