Standard name
Description Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; primary component of the Chs5/6 complex that binds directly to membranes; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.14 0 0.1 0 0 0 0 0 0 0 0 0 0 0.16 0.12 – 0 0 0 0.08 0.08 0.1
Bud 0 0 0 0.05 0 0 0.09 0.07 0.06 0.07 0.05 0.08 0.07 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.16 0.22 0.42 0.38 0.26 0.15 0.16 0.22 0.11 0.13 0.15 0.23 0.29 0.4 0.41 0.38 0.44 – 0 0.06 0.08 0 0 0
Endoplasmic Reticulum 0.06 0.06 0.1 0 0.08 0 0 0 0.05 0 0 0 0.07 0 0 0 0.06 – 0 0 0 0 0 0
Endosome 0.12 0 0 0.11 0.12 0.11 0.05 0.1 0 0 0 0 0.12 0.23 0.33 0.24 0.25 – 0.35 0.3 0.16 0.17 0.16 0.19
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 – 0.16 0.12 0 0.13 0.22 0.26
Mitochondria 0.71 0.8 0.28 0.46 0.29 0.67 0.65 0.48 0.89 0.86 0.84 0.7 0.52 0.34 0.22 0.07 0.25 – 0.25 0.34 0.55 0.45 0.37 0.27
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0 – 0.06 0 0 0.05 0.06 0.09
SpindlePole 0.06 0 0.08 0.06 0.21 0.17 0.23 0.25 0 0.08 0.07 0.16 0 0 0 0.12 0 – 0.06 0.06 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.13 0.12 – 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 2 15 3 14 5 9 8 5 2 0 3 4 1 4 11 2 – 4 5 4 13 29 44
Bud 4 4 2 6 6 11 26 25 9 14 11 18 14 8 8 0 0 – 0 0 0 2 3 2
Bud Neck 0 0 3 1 4 1 7 9 1 0 0 6 0 0 0 0 0 – 0 0 1 0 2 1
Bud Site 1 0 0 0 1 1 7 4 3 4 4 3 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 1 2 0 0 0 0 0 1 0 0 0 1 0 3 0 – 0 0 0 0 0 0
Cytoplasm 23 28 44 47 38 37 44 74 17 25 31 51 60 121 140 26 7 – 7 9 14 3 6 4
Endoplasmic Reticulum 8 8 11 2 12 7 2 1 8 1 5 0 15 5 11 3 1 – 0 1 0 1 3 11
Endosome 17 3 4 14 18 28 13 33 0 1 2 3 25 70 114 16 4 – 62 46 31 30 61 87
Golgi 4 2 1 0 0 0 0 0 0 0 0 0 2 0 3 5 1 – 29 18 7 23 84 118
Mitochondria 101 104 30 58 42 167 181 162 140 163 178 157 108 103 76 5 4 – 44 51 107 80 140 125
Nucleus 0 0 0 1 0 4 2 4 3 3 3 2 1 1 1 0 0 – 0 0 1 0 0 0
Nuclear Periphery 1 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 2
Nucleolus 2 0 0 1 0 0 1 0 0 2 1 0 2 1 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 1 2 13 9 7 0 0 0 1 6 12 17 0 0 – 10 1 8 9 23 39
SpindlePole 8 1 8 7 31 43 63 84 6 15 14 37 7 9 7 8 0 – 11 9 5 2 8 6
Vac/Vac Membrane 3 2 2 2 3 7 5 12 2 2 2 1 6 19 22 9 2 – 4 7 8 7 8 9
Unique Cell Count 142 130 106 125 147 250 277 339 158 189 211 225 208 306 342 68 16 178 154 195 178 378 459
Labelled Cell Count 177 155 121 146 171 324 369 423 194 234 251 282 250 351 403 86 21 178 154 195 178 378 459


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 4.4 5.2 5.0 5.3 4.3 4.4 4.5 3.7 3.6 3.6 4.0 5.1 5.1 5.4 8.5 7.4 – 4.9 5.0 5.6
Std Deviation (1e-4) 0.5 0.7 0.8 1.2 1.0 0.9 1.2 0.9 0.6 0.7 0.8 0.9 0.8 1.0 1.0 1.5 0.9 – 1.0 1.5 1.8
Intensity Change (Log2) – – – -0.05 0.05 -0.26 -0.22 -0.2 -0.5 -0.53 -0.53 -0.38 -0.03 -0.03 0.06 0.72 0.51 – -0.09 -0.04 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.0699 0.14 -0.0945 0.0431 0.0801 0.2328 0.1471 0.5376 0.234 0.3435 -0.1945 0.3552 0.2115 0.4451 0.1365 0.056 0.0604 0.2655
Actin 0.0544 0.0098 0.0133 0.0601 0.0015 0.018 0.0481 0.0128 0.0055 0.0047 0.0864 0.0143 0.0701 0.0156 0.0215 0.0011 0.0009 0.0023
Bud 0.0008 0.0019 0.0006 0.0009 0.0001 0.0006 0.0008 0.0006 0.0007 0.0006 0.0008 0.0008 0.0007 0.0009 0.0011 0.0006 0.0006 0.0002
Bud Neck 0.0022 0.0009 0.0021 0.0009 0.0003 0.0051 0.0011 0.0004 0.0008 0.0006 0.0027 0.002 0.0029 0.0009 0.0017 0.0007 0.0003 0.0012
Bud Periphery 0.0011 0.0026 0.0008 0.0014 0.0001 0.001 0.001 0.0004 0.0006 0.0004 0.0009 0.001 0.0011 0.0005 0.0008 0.0006 0.0006 0.0001
Bud Site 0.0033 0.0174 0.0044 0.0055 0.0004 0.0008 0.0041 0.0033 0.0131 0.001 0.0012 0.0006 0.0039 0.0173 0.0046 0.0006 0.001 0.0003
Cell Periphery 0.0005 0.0006 0.0003 0.0006 0 0.0003 0.0002 0.0001 0.0004 0.0001 0.0001 0.0002 0.0004 0.0003 0.0002 0.0001 0 0.0001
Cytoplasm 0.0691 0.1541 0.0998 0.0903 0.085 0.0617 0.0611 0.112 0.1341 0.0251 0.0236 0.0721 0.0502 0.0987 0.1116 0.0455 0.019 0.0465
Cytoplasmic Foci 0.231 0.2472 0.2357 0.1955 0.2134 0.2067 0.2584 0.2939 0.2577 0.1908 0.1967 0.275 0.2559 0.2824 0.2564 0.2723 0.286 0.2571
Eisosomes 0.001 0.0002 0.0003 0.0015 0.0001 0.0003 0.0003 0.0001 0 0 0.0001 0.0001 0.0007 0.0002 0.0002 0 0 0
Endoplasmic Reticulum 0.008 0.0054 0.0081 0.0058 0.0016 0.0037 0.0041 0.0022 0.0045 0.0016 0.0012 0.0032 0.0067 0.0023 0.0033 0.0024 0.002 0.0015
Endosome 0.3036 0.305 0.3213 0.3228 0.363 0.343 0.306 0.2976 0.3598 0.4906 0.5916 0.3146 0.3157 0.3215 0.3294 0.516 0.4203 0.3688
Golgi 0.0734 0.0869 0.0835 0.0682 0.1164 0.116 0.0999 0.1134 0.0919 0.1363 0.0632 0.1584 0.112 0.1255 0.1076 0.0981 0.1139 0.2016
Lipid Particles 0.0538 0.0337 0.0346 0.0245 0.0549 0.0377 0.0648 0.0062 0.0169 0.0074 0.0059 0.0188 0.0462 0.0237 0.0117 0.0085 0.0199 0.0358
Mitochondria 0.0194 0.0139 0.0247 0.0166 0.0114 0.0599 0.0233 0.0283 0.0098 0.0871 0.0069 0.0228 0.0136 0.0084 0.0176 0.014 0.0103 0.0115
None 0.0844 0.0688 0.1147 0.1608 0.0735 0.0783 0.0641 0.0809 0.0583 0.0008 0.0006 0.0321 0.0537 0.0537 0.0879 0.0025 0.0496 0.0234
Nuclear Periphery 0.0152 0.0022 0.0035 0.0118 0.0003 0.0016 0.0048 0.0009 0.0013 0.0004 0.0004 0.002 0.0054 0.0006 0.0013 0.0009 0.0006 0.0016
Nucleolus 0.0015 0.0003 0.0006 0.0025 0.0001 0.0002 0.0016 0.0002 0.0005 0.0001 0 0.0001 0.0011 0.0002 0.0002 0.0001 0.0002 0.0009
Nucleus 0.0157 0.001 0.0016 0.0072 0.0001 0.0005 0.0084 0.0005 0.0007 0.0002 0.0001 0.001 0.0027 0.0005 0.0005 0.0002 0.0003 0.0014
Peroxisomes 0.0474 0.0342 0.0317 0.0106 0.0728 0.0558 0.0352 0.0343 0.0297 0.0399 0.0105 0.0722 0.0351 0.0354 0.0332 0.0241 0.0529 0.0318
Punctate Nuclear 0.0068 0.0019 0.0046 0.005 0.0008 0.0016 0.0034 0.001 0.0012 0.0004 0.0004 0.0007 0.0065 0.0008 0.0015 0.0006 0.0155 0.0093
Vacuole 0.0055 0.009 0.011 0.0045 0.0027 0.0047 0.0064 0.0083 0.0099 0.007 0.0038 0.0052 0.0127 0.0087 0.0057 0.0068 0.0037 0.0029
Vacuole Periphery 0.002 0.0028 0.0029 0.003 0.0015 0.0026 0.0029 0.0026 0.0029 0.0047 0.0028 0.0026 0.0028 0.002 0.0021 0.0044 0.0024 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.8932 8.3629 10.4515 9.4663 8.6181 3.9368 12.6835 13.3664 11.2105 10.3661
Translational Efficiency 0.8034 1.1879 0.6627 0.6944 0.5592 1.7073 0.7824 0.6162 0.6346 0.7128

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘