Standard name
Human Ortholog
Description PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.97 0.97 0.98 0.89 0.88 0.85 0.84 0.73 0.77 1.0 0.99 0.97 0.91 0.93 0.94 0.97 0.94 0.91 0.93 0.92 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.22 0.07 0.27 0.29 0.32 0.42 0.51 0.54 0 0 0.11 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 0 0 1
Bud 1 0 2 2 0 3 7 13 5 13 8 0 0 4 0 0 1 0 0 0 1 2 2
Bud Neck 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0
Cell Periphery 8 7 7 3 6 4 8 4 3 6 8 1 3 4 11 15 21 0 0 1 0 0 0
Cytoplasm 225 272 365 310 319 359 431 509 259 219 273 162 186 182 286 262 312 221 269 359 104 160 182
Endoplasmic Reticulum 1 1 3 0 0 1 3 1 0 1 0 0 1 0 12 7 15 0 0 4 0 1 4
Endosome 0 0 1 0 0 0 1 5 0 0 0 0 0 1 6 2 1 0 2 1 0 1 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 0 1
Mitochondria 0 2 18 69 22 110 144 194 131 153 191 1 0 20 10 1 8 2 3 15 1 1 2
Nucleus 2 1 0 0 2 2 2 4 1 2 1 0 0 0 5 6 3 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 1 1 0 1 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 2 0 0 0 0 0 0 3 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 2 2 0 1 0 0 0 2 0 1 1 0 0 0 0 0
Vac/Vac Membrane 0 0 2 0 2 0 6 3 0 0 2 0 0 1 25 18 12 0 3 3 1 2 2
Unique Cell Count 226 274 376 320 327 403 489 598 310 298 356 162 187 188 316 282 331 228 287 394 113 175 206
Labelled Cell Count 237 284 399 384 351 481 602 738 402 396 486 164 191 213 362 312 374 228 287 394 113 175 206


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 5.8 4.4 4.5 5.0 4.2 4.0 4.1 3.6 3.7 3.4 5.8 6.0 4.9 8.8 9.0 9.0 5.7 6.3 7.1
Std Deviation (1e-4) 0.7 0.7 0.6 0.9 1.4 0.9 1.0 1.3 1.0 5.4 1.0 0.6 1.1 1.5 1.3 1.4 1.3 1.1 1.4 1.4
Intensity Change (Log2) 0.03 0.18 -0.07 -0.14 -0.1 -0.31 -0.26 -0.4 0.4 0.43 0.14 0.99 1.03 1.02 0.37 0.5 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1.3 2.4 3.0
Cytoplasm -0.2 0.4 -4.4 -4.8 -6.0 -6.1 -8.9 -8.2 2.2 1.8 -0.2 -3.6 -2.5 -1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2.7 0 3.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.1 0 0 0 0 0 0 -2.4 -3.0 2.6 -1.1 -3.4 -1.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 5.0 4.3 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2091 2.7759 3.1222 2.4928 3.0744 2.6166 3.7669 3.8487 3.9963 4.1533 4.4979 3.9121 3.995 4.9842 4.838 4.4398 3.9018 4.5835
Actin 0.0764 0.0004 0.0646 0.002 0.0689 0.0006 0.064 0.0087 0.01 0.0039 0.0139 0.0079 0.0175 0.0022 0.0057 0.0037 0.0098 0.0043
Bud 0.0015 0.0004 0.008 0.0006 0.0023 0.0009 0.0049 0.0016 0.0032 0.003 0.0011 0.002 0.0002 0.0025 0.0016 0.0002 0.0004 0.0006
Bud Neck 0.0092 0.0003 0.0048 0.0014 0.0361 0.0006 0.0096 0.0006 0.0002 0.0004 0.001 0.0033 0.0003 0.0002 0.0003 0.0002 0.0003 0.0079
Bud Periphery 0.0014 0.0003 0.0029 0.0005 0.0049 0.0006 0.0046 0.002 0.0046 0.0049 0.0014 0.0025 0.0003 0.0042 0.0017 0.0002 0.0004 0.0008
Bud Site 0.0089 0.0067 0.026 0.0003 0.0105 0.0002 0.0124 0.0163 0.0019 0.0021 0.0039 0.001 0.001 0.0036 0.0026 0.0001 0.0003 0.0019
Cell Periphery 0.0036 0.0025 0.0007 0.0007 0.0007 0.0002 0.0007 0.002 0.0003 0.0004 0.0001 0.0002 0.0003 0.0004 0.0003 0.0001 0.0001 0.0003
Cytoplasm 0.615 0.8366 0.7023 0.8506 0.6276 0.8464 0.5293 0.8569 0.7646 0.616 0.5441 0.8144 0.7073 0.8245 0.8024 0.874 0.7221 0.8474
Cytoplasmic Foci 0.063 0.0078 0.0192 0.0125 0.009 0.0079 0.0521 0.0082 0.0146 0.0306 0.0327 0.0137 0.0186 0.0056 0.0142 0.0044 0.0169 0.0063
Eisosomes 0.0005 0.0001 0.0006 0 0.0006 0 0.0005 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0013 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0097 0.007 0.0071 0.0608 0.0079 0.0043 0.0103 0.0054 0.0042 0.0098 0.0039 0.0051 0.0032 0.0008 0.0044 0.0043 0.0025 0.0053
Endosome 0.0176 0.0031 0.0087 0.026 0.0205 0.0047 0.0509 0.0057 0.0273 0.0446 0.0457 0.016 0.0203 0.0021 0.0075 0.0192 0.0196 0.0054
Golgi 0.0129 0.0006 0.0074 0.003 0.0064 0.0024 0.0208 0.0009 0.002 0.017 0.0318 0.0064 0.0072 0.0003 0.0026 0.0087 0.0325 0.0013
Lipid Particles 0.0108 0.0006 0.0036 0.0044 0.0405 0.0051 0.0169 0.0002 0.002 0.01 0.0208 0.002 0.012 0 0.0039 0.0036 0.0005 0.0001
Mitochondria 0.0021 0.0002 0.0019 0.0005 0.0265 0.0143 0.0161 0.0006 0.0015 0.0098 0.0208 0.005 0.0012 0.0003 0.0014 0.0618 0.005 0.0027
None 0.0937 0.1292 0.1134 0.0196 0.07 0.1075 0.1505 0.0843 0.1416 0.2142 0.2303 0.1106 0.1916 0.151 0.1429 0.0091 0.1863 0.1128
Nuclear Periphery 0.0037 0.0005 0.007 0.0034 0.0379 0.0002 0.014 0.0011 0.0034 0.006 0.0034 0.0035 0.0032 0.0001 0.001 0.0008 0.0003 0.0003
Nucleolus 0.0009 0.0001 0.0006 0.0001 0.0014 0.0001 0.0016 0.0001 0.0001 0.0003 0.0006 0.0001 0.0004 0 0.0001 0 0 0
Nucleus 0.0015 0.0007 0.007 0.0023 0.0077 0.0004 0.0078 0.0011 0.0015 0.0031 0.0011 0.0012 0.0007 0.0005 0.0008 0.0005 0.0004 0.0004
Peroxisomes 0.0199 0.0001 0.0044 0.0001 0.0077 0.0009 0.02 0.0002 0.0075 0.0148 0.0348 0.0014 0.0084 0.0001 0.0024 0.0009 0.0009 0.0001
Punctate Nuclear 0.0433 0.0002 0.0069 0.0005 0.0018 0.0002 0.0054 0.0004 0.0013 0.0018 0.0027 0.0009 0.0031 0.0001 0.0009 0.0001 0.0003 0.0001
Vacuole 0.004 0.0027 0.0025 0.0097 0.0057 0.002 0.0056 0.0032 0.0071 0.0059 0.0047 0.002 0.0023 0.0013 0.0018 0.0058 0.0009 0.0016
Vacuole Periphery 0.0006 0.0001 0.0004 0.0009 0.0054 0.0004 0.002 0.0004 0.0012 0.0014 0.0011 0.0006 0.0007 0.0002 0.0003 0.0021 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.3618 15.813 16.3833 15.9371 21.4202 15.3444 21.7203 18.7319 18.3867 24.8597
Translational Efficiency 0.7944 0.833 0.5868 0.4729 0.4738 0.8775 0.5597 0.457 0.3973 0.4775

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1847 513 153 1224 1897 1534 163 142 3744 2047 316 1366

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 741.73 760.40 1106.02 828.89 753.25 776.14 996.02 847.43 747.57 772.20 1049.28 830.82
Standard Deviation 87.89 127.18 149.99 120.18 110.18 126.50 104.72 98.72 99.97 126.86 139.90 118.27
Intensity Change Log 2 0.035864 0.576411 0.160287 0.043188 0.403046 0.169965 0.039559 0.491664 0.165171

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000166 0.000281 0.000916 0.001561 0.000174 0.000693 0.000448 0.000443 0.000170 0.000589 0.000675 0.001445
Bud Neck 0.010011 0.015318 0.001308 0.007964 0.008870 0.013372 0.002888 0.034948 0.009433 0.013860 0.002123 0.010769
Bud Site 0.012509 0.035779 0.006174 0.033554 0.010070 0.022731 0.003393 0.029187 0.011273 0.026001 0.004739 0.033100
Cell Periphery 0.000172 0.000128 0.000301 0.000197 0.000084 0.000116 0.000209 0.000165 0.000127 0.000119 0.000253 0.000194
Cytoplasm 0.641851* 0.511910* 0.535820* 0.492475* 0.638531* 0.591165* 0.245138* 0.389744* 0.640169* 0.571303* 0.385880* 0.481796*
Cytoplasmic Foci 0.188364* 0.242561* 0.033492 0.037115 0.165196 0.171579 0.009528 0.059053 0.176625 0.189368* 0.021131 0.039395
Eisosomes 0.000094 0.000074 0.000054 0.000067 0.000062 0.000070 0.000091 0.000074 0.000078 0.000071 0.000073 0.000067
Endoplasmic Reticulum 0.000761 0.000693 0.005546 0.001402 0.000914 0.000726 0.007469 0.002272 0.000839 0.000718 0.006538 0.001493
Endosome 0.013237 0.046084 0.005291 0.014810 0.013332 0.028184 0.005751 0.028884 0.013285 0.032670 0.005528 0.016273
Golgi 0.006933 0.007901 0.000329 0.008504 0.004868 0.006343 0.000103 0.001610 0.005887 0.006733 0.000213 0.007787
Lipid Particles 0.002166 0.003205 0.001887 0.001599 0.002636 0.002424 0.002824 0.002098 0.002404 0.002620 0.002370 0.001651
Mitochondria 0.002755 0.003772 0.000202 0.005465 0.003011 0.003330 0.000448 0.017784 0.002884 0.003441 0.000329 0.006746
Mitotic Spindle 0.000644 0.000601 0.001991 0.051786 0.002895 0.006426 0.021897 0.014216 0.001784 0.004966 0.012259 0.047881
None 0.001496 0.001334 0.003847 0.005086 0.002024 0.001602 0.006745 0.002118 0.001764 0.001535 0.005342 0.004777
Nuclear Periphery 0.000070 0.000227 0.000718 0.000929 0.000389 0.000307 0.002670 0.000771 0.000232 0.000287 0.001725 0.000913
Nuclear Periphery Foci 0.000210 0.000581 0.011103 0.001337 0.000340 0.000539 0.004388 0.001705 0.000276 0.000549 0.007639 0.001376
Nucleolus 0.000339 0.000717 0.000228 0.001340 0.000561 0.002030 0.000780 0.000416 0.000452 0.001701 0.000513 0.001244
Nucleus 0.031581 0.019636 0.251398* 0.135512* 0.051765 0.025263 0.468737* 0.080893 0.041808 0.023853 0.363507* 0.129834
Peroxisomes 0.001554 0.006317 0.000323 0.003190 0.001761 0.003871 0.000260 0.003475 0.001659 0.004484 0.000290 0.003220
Vacuole 0.082661 0.099229 0.138793 0.186857 0.090240 0.114853 0.216054* 0.325994* 0.086501 0.110938 0.178646 0.201321*
Vacuole Periphery 0.002428 0.003654 0.000280 0.009249 0.002276 0.004376 0.000178 0.004150 0.002351 0.004195 0.000227 0.008719

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.68 -6.80 -1.75 -1.50 -0.06 -2.83 -6.26 -1.61 1.89 3.50 -3.06 -9.22 -1.78 -0.71 -0.15
Bud Neck -2.94 13.98 4.09 5.06 -5.51 -4.06 4.70 -0.47 0.42 -1.31 -4.80 11.39 3.01 6.14 -3.55
Bud Site -7.65 7.08 -3.34 4.17 -5.97 -6.96 5.11 -1.07 1.47 -2.11 -9.58 8.47 -4.01 2.44 -6.65
Cell Periphery 0.77 -1.93 0.78 -0.04 5.23 -2.36 -6.54 -0.65 1.29 4.91 0.29 -3.68 0.00 -0.57 6.03
Cytoplasm 9.55 7.67 21.28 5.67 2.44 5.00 27.79 13.17 11.08 -4.42 9.16 19.97 24.82 15.07 -5.01
Cytoplasmic Foci -5.65 32.01 38.90 24.68 -0.20 -1.09 39.15 22.64 22.38 -5.82 -2.54 49.01 48.18 37.98 -5.32
Eisosomes 2.65 4.43 5.45 1.68 -0.63 -1.39 -5.73 -1.10 0.02 4.21 1.62 -1.27 3.65 1.46 3.86
Endoplasmic Reticulum 0.32 -6.73 -2.97 -3.58 5.63 0.99 -10.27 -3.27 -3.40 9.09 0.75 -11.93 -3.61 -3.72 10.53
Endosome -7.63 8.80 4.44 8.78 -2.27 -8.92 9.48 1.22 5.46 -1.50 -11.84 12.94 4.94 13.53 -2.89
Golgi -0.59 17.01 2.69 2.22 -3.20 -1.90 14.89 10.84 7.68 -1.43 -1.18 22.55 2.37 2.74 -3.31
Lipid Particles -1.33 0.73 3.57 2.28 2.07 0.50 -0.58 3.74 2.41 3.92 -0.76 0.01 5.46 3.76 4.65
Mitochondria -0.65 3.64 -0.02 0.66 -4.51 -0.34 3.65 -0.41 -0.34 -1.01 -0.72 5.15 -0.12 0.50 -3.89
Mitotic Spindle 0.14 -1.12 -8.32 -8.35 -7.82 -2.04 -1.28 -2.08 -1.67 -0.94 -2.67 -1.44 -8.27 -7.17 -5.25
None 0.30 -1.16 -3.07 -2.83 -0.82 0.74 -2.11 -1.18 -1.61 1.10 0.57 -2.38 -2.87 -3.04 0.03
Nuclear Periphery -1.25 -10.93 -14.56 -7.55 -4.64 0.58 -11.94 -4.55 -5.77 9.01 -0.52 -12.65 -11.04 -9.77 5.20
Nuclear Periphery Foci -2.85 -10.86 -5.07 -2.26 9.96 -1.42 -6.30 -4.86 -3.31 4.31 -2.53 -11.82 -5.34 -2.87 10.28
Nucleolus -1.96 2.58 -2.36 -0.30 -3.16 -2.42 -0.21 3.16 2.90 1.80 -2.74 0.43 -1.65 1.99 -1.73
Nucleus 4.46 -11.08 -24.12 -24.68 3.80 9.86 -17.09 -4.32 -6.48 12.94 9.72 -18.97 -22.52 -25.92 11.14
Peroxisomes -6.65 12.61 -0.51 4.60 -2.49 -4.29 9.57 0.93 3.72 -1.84 -6.91 14.74 -0.27 3.84 -2.70
Vacuole -3.04 -7.18 -25.07 -19.63 -3.89 -5.35 -9.72 -13.75 -12.80 -4.61 -6.99 -11.90 -27.75 -23.42 -2.77
Vacuole Periphery -2.14 10.26 -1.67 -0.50 -3.81 -2.97 7.68 1.53 3.43 -1.46 -3.43 12.34 -1.62 0.26 -3.94