Standard name | |
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Description | PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud | 1 | 0 | 2 | 2 | 0 | 3 | 7 | 13 | 5 | – | 13 | 8 | 0 | 0 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 7 | 7 | 3 | 6 | 4 | 8 | 4 | 3 | – | 6 | 8 | 1 | 3 | 4 | 11 | 15 | 21 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 225 | 272 | 365 | 310 | 319 | 359 | 431 | 509 | 259 | – | 219 | 273 | 162 | 186 | 182 | 286 | 262 | 312 | 221 | 269 | 359 | 104 | 160 | 182 |
Endoplasmic Reticulum | 1 | 1 | 3 | 0 | 0 | 1 | 3 | 1 | 0 | – | 1 | 0 | 0 | 1 | 0 | 12 | 7 | 15 | 0 | 0 | 4 | 0 | 1 | 4 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 5 | 0 | – | 0 | 0 | 0 | 0 | 1 | 6 | 2 | 1 | 0 | 2 | 1 | 0 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 |
Mitochondria | 0 | 2 | 18 | 69 | 22 | 110 | 144 | 194 | 131 | – | 153 | 191 | 1 | 0 | 20 | 10 | 1 | 8 | 2 | 3 | 15 | 1 | 1 | 2 |
Nucleus | 2 | 1 | 0 | 0 | 2 | 2 | 2 | 4 | 1 | – | 2 | 1 | 0 | 0 | 0 | 5 | 6 | 3 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | – | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 2 | 0 | 2 | 0 | 6 | 3 | 0 | – | 0 | 2 | 0 | 0 | 1 | 25 | 18 | 12 | 0 | 3 | 3 | 1 | 2 | 2 |
Unique Cell Count | 226 | 274 | 376 | 320 | 327 | 403 | 489 | 598 | 310 | 298 | 356 | 162 | 187 | 188 | 316 | 282 | 331 | 228 | 287 | 394 | 113 | 175 | 206 | |
Labelled Cell Count | 237 | 284 | 399 | 384 | 351 | 481 | 602 | 738 | 402 | 396 | 486 | 164 | 191 | 213 | 362 | 312 | 374 | 228 | 287 | 394 | 113 | 175 | 206 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 5.8 | 4.4 | 4.5 | 5.0 | 4.2 | 4.0 | 4.1 | 3.6 | – | 3.7 | 3.4 | 5.8 | 6.0 | 4.9 | 8.8 | 9.0 | 9.0 | 5.7 | 6.3 | 7.1 |
Std Deviation (1e-4) | 0.7 | 0.7 | 0.6 | 0.9 | 1.4 | 0.9 | 1.0 | 1.3 | 1.0 | – | 5.4 | 1.0 | 0.6 | 1.1 | 1.5 | 1.3 | 1.4 | 1.3 | 1.1 | 1.4 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.18 | -0.07 | -0.14 | -0.1 | -0.31 | – | -0.26 | -0.4 | 0.4 | 0.43 | 0.14 | 0.99 | 1.03 | 1.02 | 0.37 | 0.5 | 0.68 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.3 | 2.4 | 3.0 |
Cytoplasm | -0.2 | 0.4 | -4.4 | -4.8 | -6.0 | -6.1 | – | -8.9 | -8.2 | 2.2 | 1.8 | -0.2 | -3.6 | -2.5 | -1.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.7 | 0 | 3.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1.1 | 0 | 0 | 0 | 0 | – | 0 | 0 | -2.4 | -3.0 | 2.6 | -1.1 | -3.4 | -1.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 5.0 | 4.3 | 2.9 |