Standard name
Description PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.97 0.97 0.98 0.89 0.88 0.85 0.84 – 0.73 0.77 1.0 0.99 0.97 0.91 0.93 0.94 0.97 0.94 0.91 0.93 0.92 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.22 0.07 0.27 0.29 0.32 0.42 – 0.51 0.54 0 0 0.11 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.08 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 1 1 0 0 0 1 0 0 1
Bud 1 0 2 2 0 3 7 13 5 – 13 8 0 0 4 0 0 1 0 0 0 1 2 2
Bud Neck 0 0 0 0 0 1 0 0 0 – 1 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 1 – 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 8 7 7 3 6 4 8 4 3 – 6 8 1 3 4 11 15 21 0 0 1 0 0 0
Cytoplasm 225 272 365 310 319 359 431 509 259 – 219 273 162 186 182 286 262 312 221 269 359 104 160 182
Endoplasmic Reticulum 1 1 3 0 0 1 3 1 0 – 1 0 0 1 0 12 7 15 0 0 4 0 1 4
Endosome 0 0 1 0 0 0 1 5 0 – 0 0 0 0 1 6 2 1 0 2 1 0 1 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 1 1 0 0 1
Mitochondria 0 2 18 69 22 110 144 194 131 – 153 191 1 0 20 10 1 8 2 3 15 1 1 2
Nucleus 2 1 0 0 2 2 2 4 1 – 2 1 0 0 0 5 6 3 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 – 1 1 0 1 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 2 0 – 0 0 0 0 0 3 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 2 2 – 0 1 0 0 0 2 0 1 1 0 0 0 0 0
Vac/Vac Membrane 0 0 2 0 2 0 6 3 0 – 0 2 0 0 1 25 18 12 0 3 3 1 2 2
Unique Cell Count 226 274 376 320 327 403 489 598 310 298 356 162 187 188 316 282 331 228 287 394 113 175 206
Labelled Cell Count 237 284 399 384 351 481 602 738 402 396 486 164 191 213 362 312 374 228 287 394 113 175 206


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 5.8 4.4 4.5 5.0 4.2 4.0 4.1 3.6 – 3.7 3.4 5.8 6.0 4.9 8.8 9.0 9.0 5.7 6.3 7.1
Std Deviation (1e-4) 0.7 0.7 0.6 0.9 1.4 0.9 1.0 1.3 1.0 – 5.4 1.0 0.6 1.1 1.5 1.3 1.4 1.3 1.1 1.4 1.4
Intensity Change (Log2) – – – 0.03 0.18 -0.07 -0.14 -0.1 -0.31 – -0.26 -0.4 0.4 0.43 0.14 0.99 1.03 1.02 0.37 0.5 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 1.3 2.4 3.0
Cytoplasm -0.2 0.4 -4.4 -4.8 -6.0 -6.1 – -8.9 -8.2 2.2 1.8 -0.2 -3.6 -2.5 -1.9
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 2.7 0 3.1
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 1.1 0 0 0 0 – 0 0 -2.4 -3.0 2.6 -1.1 -3.4 -1.7
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 5.0 4.3 2.9