Standard name
Human Ortholog
Description Nucleolar protein involved in pre-25S rRNA processing; also involved in biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0.13 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.06 0.05 0 0 0.1 0.1 0.06 0.13 0.18 0.17 0.18 0.08 0 0 0.12 0.07 0.11 0 0 0 0 0 0
Nucleus 0.26 0.27 0.23 0 0 0 0 0 0.05 0 0.05 0.07 0.07 0 0 0.11 0.14 0.17 0.14 0.25 0.11 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.76 0.65 0.77 0.93 0.92 0.89 0.87 0.83 0.93 0.84 0.84 0.8 0.87 0.84 0.81 0.88 0.88 0.86 0.76 0.64 0.74 0.82 0.68 0.6
Peroxisomes 0 0 0 0 0 0.07 0.09 0.05 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.15 0.29 0.32 0.4 0.42 0.25 0.3 0.27 0.3 0 0.06 0.09 0 0 0 0 0 0 0.05 0.17 0.12
Vac/Vac Membrane 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 1 1 4 2 4 1 1 4 1 0 0 0 0 0 2 4 1 3 2 6
Bud Neck 0 0 1 15 10 8 13 16 7 4 6 7 4 31 16 0 1 0 0 1 0 0 4 5
Bud Site 0 0 0 1 0 3 13 9 4 19 10 20 0 1 1 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 6 3 2 6 5 8 5 7 2 13 7 12 19 57 76 13 4 2 2 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1 0 0 0
Endosome 2 0 1 4 16 8 41 15 10 13 8 8 2 12 23 4 0 1 5 2 4 2 10 32
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 4
Mitochondria 35 12 9 6 11 32 55 32 39 59 41 78 22 10 8 40 13 13 1 0 1 3 5 5
Nucleus 121 52 44 10 10 7 15 21 14 12 13 29 19 18 13 37 27 21 76 51 22 15 17 15
Nuclear Periphery 1 0 1 0 1 0 2 2 1 1 1 0 0 0 0 0 0 1 5 0 1 1 2 5
Nucleolus 360 127 146 274 293 281 503 481 273 275 208 354 240 467 464 299 166 104 403 130 152 216 287 258
Peroxisomes 1 0 0 6 9 23 51 29 5 16 10 21 1 12 14 2 0 1 0 0 0 0 3 6
SpindlePole 1 1 1 44 92 100 229 244 74 98 66 133 8 35 51 3 1 2 11 3 6 12 72 53
Vac/Vac Membrane 15 19 5 0 2 1 5 2 1 3 1 1 5 10 7 2 0 1 16 9 8 6 7 26
Unique Cell Count 472 194 190 294 318 317 578 580 293 329 248 445 277 554 570 338 188 121 532 205 206 265 421 432
Labelled Cell Count 542 214 210 366 450 472 936 860 434 514 372 667 321 653 673 404 212 146 532 205 206 265 421 432


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.0 19.8 17.0 16.9 13.6 10.2 9.1 9.2 9.2 8.3 8.3 8.5 19.2 18.4 16.7 25.1 29.5 31.5 20.0 18.7 19.5
Std Deviation (1e-4) 4.7 4.6 4.0 4.2 3.3 2.6 2.6 2.4 2.2 2.7 2.5 2.6 5.7 6.6 6.3 8.6 12.0 12.0 4.6 4.3 4.9
Intensity Change (Log2) – – – -0.01 -0.32 -0.74 -0.9 -0.89 -0.89 -1.04 -1.04 -1.0 0.17 0.11 -0.03 0.56 0.79 0.89 0.23 0.14 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 2.7 0 0 0 0 0 0 0 0 0 3.0 0 0 0 0
Bud Site 0 0 0 0 0 0 3.4 0 3.0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 1.9 0 0 3.0 4.1 4.8 1.9 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.7 0 3.5 0 0 2.3 0 0 0 0 2.4 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.7 -0.7 2.1 2.1 0.4 3.1 4.3 3.8 4.3 1.4 0 0 2.7 0.9 2.0
Nucleus -6.7 -7.1 -7.6 -9.2 -8.4 -6.1 -6.9 -5.5 -6.0 -5.1 -8.6 -9.5 -3.7 -2.2 -1.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 5.2 4.9 3.5 3.4 1.9 5.2 1.9 1.9 0.8 2.7 2.3 1.4 3.5 2.9 2.0
Peroxisomes 0 0 3.8 4.2 3.1 0 3.1 0 3.0 0 0 0 0 0 0
SpindlePole 5.3 8.0 8.5 10.2 10.7 7.3 8.2 7.5 8.3 0 3.2 4.0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.2207 16.7337 15.6223 15.4559 15.0647 15.1152 13.0564 16.1769 13.9097 12.9509 13.6273 13.9746 16.8355 21.4501 19.475 18.0858 18.5624 18.8344
Actin 0.0125 0 0.0007 0 0.0002 0 0.0164 0.0084 0.0168 0.0747 0.0361 0.0018 0.0065 0 0.0022 0.0005 0.0004 0.0077
Bud 0.0001 0 0 0.0001 0 0.0001 0.0002 0.0003 0.0003 0.0001 0.0007 0.0001 0.0002 0 0.0001 0.0001 0.0004 0
Bud Neck 0.0002 0.0003 0.0003 0.0002 0.0001 0.0009 0.0005 0.0002 0.0005 0.0007 0.0005 0.0003 0.0002 0.0002 0.0002 0.0004 0.0017 0.0003
Bud Periphery 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0004 0.0003 0.0004 0.0002 0.0013 0.0002 0.0003 0.0001 0.0003 0.0002 0.0014 0.0001
Bud Site 0.0003 0.0002 0.0002 0.0001 0.0001 0.0003 0.0008 0.0013 0.0012 0.0005 0.0017 0.0002 0.0011 0.0001 0.0015 0.0003 0.0007 0.0001
Cell Periphery 0.0001 0.0002 0.0001 0.0001 0.0002 0.0002 0.0003 0.0001 0.0002 0.0002 0.0005 0.0002 0.0004 0.0002 0.0003 0.0005 0.0019 0.0002
Cytoplasm 0.0001 0 0.0001 0 0 0 0.0003 0.0016 0.0009 0.0001 0.0002 0.0007 0.0079 0 0.0003 0.0001 0.0003 0
Cytoplasmic Foci 0.0043 0.0012 0.0021 0.0001 0.0013 0.0023 0.0078 0.0025 0.0121 0.023 0.0084 0.0111 0.016 0.0049 0.0068 0.0109 0.0055 0.0008
Eisosomes 0.0001 0 0 0 0.0001 0 0.0003 0.0001 0.0001 0.0001 0.0002 0 0.0002 0 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0001 0 0 0 0 0 0.0021 0.0022 0.0013 0 0.0003 0.0002 0.0086 0 0.0006 0.0001 0.0001 0
Endosome 0.0011 0.0001 0.0009 0 0.0001 0.0011 0.0038 0.0141 0.0263 0.0007 0.0051 0.008 0.0146 0 0.0023 0.0006 0.001 0.0002
Golgi 0.0016 0.0001 0.0003 0 0.0004 0.0005 0.0026 0.0033 0.0101 0.0028 0.0107 0.0074 0.0052 0 0.0004 0.0004 0.0011 0.0002
Lipid Particles 0.0089 0.0024 0.0057 0.0002 0.029 0.0118 0.0232 0.0049 0.0156 0.068 0.0235 0.0271 0.0085 0.0021 0.0047 0.0154 0.0279 0.0114
Mitochondria 0.0015 0.0001 0.0004 0.0001 0.001 0.0016 0.0032 0.0113 0.012 0.0003 0.0074 0.0017 0.0043 0.0025 0.0058 0.0089 0.0065 0.001
None 0.0007 0 0.0011 0 0.0001 0 0.0011 0.0003 0.001 0.0001 0.0005 0.0006 0.0071 0 0.0008 0.0004 0.0006 0.0001
Nuclear Periphery 0.0113 0.0004 0.0007 0.0002 0.0133 0.0045 0.0055 0.0264 0.0076 0.0001 0.0483 0.0079 0.013 0.0005 0.0028 0.0292 0.0118 0.0096
Nucleolus 0.8514 0.8417 0.9157 0.9026 0.8735 0.8732 0.7774 0.7806 0.8108 0.6695 0.7178 0.8087 0.7963 0.8625 0.8983 0.8353 0.7881 0.8466
Nucleus 0.0866 0.1409 0.0662 0.0946 0.0713 0.0946 0.1366 0.1284 0.0699 0.1488 0.0943 0.1107 0.0811 0.1249 0.0678 0.0828 0.1274 0.1156
Peroxisomes 0.0024 0.0001 0.0018 0 0.0003 0.0004 0.0079 0.0029 0.0054 0.0072 0.0116 0.0032 0.0047 0.0006 0.0004 0.0018 0.0006 0.0001
Punctate Nuclear 0.0155 0.0117 0.0026 0.0011 0.0064 0.0065 0.0073 0.0059 0.0035 0.0024 0.023 0.009 0.0152 0.0008 0.0035 0.0031 0.0113 0.0039
Vacuole 0.0003 0.0004 0.0004 0.0002 0.0003 0.0009 0.001 0.0026 0.0021 0.0003 0.0016 0.0004 0.0025 0.0004 0.0004 0.0031 0.0045 0.0006
Vacuole Periphery 0.0007 0.0001 0.0003 0.0001 0.0022 0.0007 0.0012 0.0023 0.002 0.0001 0.0062 0.0007 0.0061 0.0004 0.0004 0.006 0.0066 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 80.7564 60.0403 62.0552 56.8567 71.5306 49.5458 52.9377 43.1679 46.2123 57.3447
Translational Efficiency 2.5596 3.1679 2.4738 2.6443 2.2422 2.5283 1.8904 2.2602 1.7335 2.1892

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nucleolar protein involved in pre-25S rRNA processing; also involved in biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p
Localization
Cell Percentages nucleolus (53%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Nop12

Nop12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nop12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available