Standard name
Description Nucleolar protein involved in pre-25S rRNA processing; also involved in biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0.13 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.06 0.05 0 0 0.1 0.1 0.06 0.13 0.18 0.17 0.18 0.08 0 0 0.12 0.07 0.11 0 0 0 0 0 0
Nucleus 0.26 0.27 0.23 0 0 0 0 0 0.05 0 0.05 0.07 0.07 0 0 0.11 0.14 0.17 0.14 0.25 0.11 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.76 0.65 0.77 0.93 0.92 0.89 0.87 0.83 0.93 0.84 0.84 0.8 0.87 0.84 0.81 0.88 0.88 0.86 0.76 0.64 0.74 0.82 0.68 0.6
Peroxisomes 0 0 0 0 0 0.07 0.09 0.05 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.15 0.29 0.32 0.4 0.42 0.25 0.3 0.27 0.3 0 0.06 0.09 0 0 0 0 0 0 0.05 0.17 0.12
Vac/Vac Membrane 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 1 1 4 2 4 1 1 4 1 0 0 0 0 0 2 4 1 3 2 6
Bud Neck 0 0 1 15 10 8 13 16 7 4 6 7 4 31 16 0 1 0 0 1 0 0 4 5
Bud Site 0 0 0 1 0 3 13 9 4 19 10 20 0 1 1 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 6 3 2 6 5 8 5 7 2 13 7 12 19 57 76 13 4 2 2 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1 0 0 0
Endosome 2 0 1 4 16 8 41 15 10 13 8 8 2 12 23 4 0 1 5 2 4 2 10 32
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 4
Mitochondria 35 12 9 6 11 32 55 32 39 59 41 78 22 10 8 40 13 13 1 0 1 3 5 5
Nucleus 121 52 44 10 10 7 15 21 14 12 13 29 19 18 13 37 27 21 76 51 22 15 17 15
Nuclear Periphery 1 0 1 0 1 0 2 2 1 1 1 0 0 0 0 0 0 1 5 0 1 1 2 5
Nucleolus 360 127 146 274 293 281 503 481 273 275 208 354 240 467 464 299 166 104 403 130 152 216 287 258
Peroxisomes 1 0 0 6 9 23 51 29 5 16 10 21 1 12 14 2 0 1 0 0 0 0 3 6
SpindlePole 1 1 1 44 92 100 229 244 74 98 66 133 8 35 51 3 1 2 11 3 6 12 72 53
Vac/Vac Membrane 15 19 5 0 2 1 5 2 1 3 1 1 5 10 7 2 0 1 16 9 8 6 7 26
Unique Cell Count 472 194 190 294 318 317 578 580 293 329 248 445 277 554 570 338 188 121 532 205 206 265 421 432
Labelled Cell Count 542 214 210 366 450 472 936 860 434 514 372 667 321 653 673 404 212 146 532 205 206 265 421 432


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.0 19.8 17.0 16.9 13.6 10.2 9.1 9.2 9.2 8.3 8.3 8.5 19.2 18.4 16.7 25.1 29.5 31.5 20.0 18.7 19.5
Std Deviation (1e-4) 4.7 4.6 4.0 4.2 3.3 2.6 2.6 2.4 2.2 2.7 2.5 2.6 5.7 6.6 6.3 8.6 12.0 12.0 4.6 4.3 4.9
Intensity Change (Log2) – – – -0.01 -0.32 -0.74 -0.9 -0.89 -0.89 -1.04 -1.04 -1.0 0.17 0.11 -0.03 0.56 0.79 0.89 0.23 0.14 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 2.7 0 0 0 0 0 0 0 0 0 3.0 0 0 0 0
Bud Site 0 0 0 0 0 0 3.4 0 3.0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 1.9 0 0 3.0 4.1 4.8 1.9 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.7 0 3.5 0 0 2.3 0 0 0 0 2.4 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.7 -0.7 2.1 2.1 0.4 3.1 4.3 3.8 4.3 1.4 0 0 2.7 0.9 2.0
Nucleus -6.7 -7.1 -7.6 -9.2 -8.4 -6.1 -6.9 -5.5 -6.0 -5.1 -8.6 -9.5 -3.7 -2.2 -1.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 5.2 4.9 3.5 3.4 1.9 5.2 1.9 1.9 0.8 2.7 2.3 1.4 3.5 2.9 2.0
Peroxisomes 0 0 3.8 4.2 3.1 0 3.1 0 3.0 0 0 0 0 0 0
SpindlePole 5.3 8.0 8.5 10.2 10.7 7.3 8.2 7.5 8.3 0 3.2 4.0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0