Standard name | |
---|---|
Human Ortholog | |
Description | Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 3 |
Bud | 0 | 0 | 1 | 0 | 1 | 4 | 5 | 8 | 8 | 4 | 4 | 6 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 9 | 1 | 5 | 15 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 1 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 67 | 165 | 232 | 138 | 210 | 258 | 261 | 220 | 215 | 236 | 167 | 186 | 81 | 112 | 138 | 77 | 87 | 85 | 85 | 157 | 270 | 57 | 179 | 192 |
Endoplasmic Reticulum | 1 | 2 | 2 | 0 | 3 | 2 | 2 | 2 | 3 | 4 | 2 | 2 | 4 | 0 | 3 | 2 | 2 | 4 | 0 | 0 | 26 | 1 | 11 | 10 |
Endosome | 0 | 1 | 6 | 8 | 7 | 5 | 9 | 2 | 7 | 2 | 3 | 11 | 0 | 1 | 3 | 0 | 2 | 1 | 1 | 1 | 16 | 0 | 10 | 16 |
Golgi | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 0 | 1 | 0 | 1 | 0 |
Mitochondria | 3 | 5 | 12 | 22 | 23 | 106 | 199 | 171 | 174 | 178 | 181 | 142 | 0 | 0 | 0 | 3 | 3 | 1 | 1 | 3 | 4 | 4 | 20 | 11 |
Nucleus | 4 | 6 | 8 | 13 | 42 | 36 | 67 | 65 | 89 | 107 | 81 | 85 | 5 | 5 | 0 | 2 | 5 | 6 | 25 | 49 | 53 | 4 | 19 | 16 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 3 | 3 | 5 | 8 | 4 | 2 | 2 | 10 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 31 | 1 | 6 | 6 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 2 | 7 | 6 | 2 | 3 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 8 | 1 | 6 | 9 |
Vac/Vac Membrane | 50 | 93 | 258 | 198 | 288 | 263 | 406 | 322 | 387 | 390 | 263 | 371 | 26 | 89 | 108 | 103 | 114 | 109 | 16 | 23 | 37 | 10 | 52 | 63 |
Unique Cell Count | 111 | 243 | 444 | 326 | 503 | 583 | 791 | 694 | 755 | 785 | 596 | 687 | 106 | 182 | 227 | 169 | 184 | 181 | 139 | 256 | 478 | 89 | 327 | 359 |
Labelled Cell Count | 127 | 272 | 522 | 379 | 578 | 680 | 962 | 808 | 893 | 929 | 706 | 820 | 116 | 207 | 252 | 191 | 215 | 210 | 139 | 256 | 478 | 89 | 327 | 359 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 5.9 | 6.6 | 6.1 | 6.6 | 6.2 | 6.7 | 6.7 | 7.6 | 7.5 | 7.9 | 8.2 | 6.1 | 7.0 | 7.6 | 8.8 | 8.8 | 8.6 | – | 6.4 | 7.1 |
Std Deviation (1e-4) | 1.6 | 1.1 | 1.7 | 1.4 | 1.5 | 1.8 | 2.0 | 1.8 | 2.2 | 2.3 | 2.7 | 2.8 | 1.2 | 1.5 | 1.1 | 3.0 | 2.0 | 3.0 | – | 1.2 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.02 | -0.09 | 0.01 | 0.02 | 0.2 | 0.18 | 0.25 | 0.3 | -0.11 | 0.08 | 0.2 | 0.41 | 0.41 | 0.38 | – | -0.04 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.7 | -3.2 | -2.5 | -6.6 | -6.9 | -8.2 | -7.7 | -7.9 | -8.6 | 4.5 | 2.1 | 2.1 | -1.5 | -1.1 | -1.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1.1 | 0.1 | 0 | -0.3 | 0 | 0 | 0 | 0 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.7 | 1.5 | 7.7 | 10.1 | 9.8 | 9.4 | 9.3 | 11.4 | 8.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.8 | 4.5 | 3.4 | 4.7 | 5.1 | 6.1 | 6.8 | 6.7 | 6.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.7 | -0.3 | -4.1 | -2.3 | -3.9 | -2.3 | -2.8 | -4.5 | -1.4 | -6.2 | -2.1 | -2.6 | 0.6 | 0.9 | 0.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.2989 | 0.1636 | 0.0824 | -0.2531 | -0.2533 | 0.1898 | 1.4756 | 1.8449 | 1.6052 | 1.0876 | 1.4688 | 1.7665 | 0.2016 | 0.683 | 0.4367 | 0.2948 | 0.549 | 0.314 |
Actin | 0.0063 | 0.0009 | 0.0006 | 0.0005 | 0.0146 | 0.0017 | 0.0067 | 0.0015 | 0.008 | 0.0332 | 0.0288 | 0.0014 | 0.017 | 0.0001 | 0.0029 | 0.0009 | 0.0085 | 0.0049 |
Bud | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0015 | 0.0001 | 0.0004 | 0.0017 | 0.0011 | 0.0003 | 0.0003 | 0.0008 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0009 | 0.0006 |
Bud Neck | 0.0007 | 0.0005 | 0.0003 | 0.0007 | 0.0009 | 0.001 | 0.005 | 0.0003 | 0.0007 | 0.0021 | 0.0008 | 0.0017 | 0.0009 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0004 |
Bud Periphery | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0038 | 0 | 0.0004 | 0.0013 | 0.0008 | 0.0004 | 0.0002 | 0.0016 | 0.0002 | 0 | 0.0006 | 0.0001 | 0.0006 | 0.005 |
Bud Site | 0.0002 | 0.0112 | 0.0005 | 0.0002 | 0.0088 | 0.0001 | 0.0073 | 0.0047 | 0.0069 | 0.0013 | 0.0006 | 0.0002 | 0.0016 | 0.0004 | 0.0006 | 0.0002 | 0.0018 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.5047 | 0.5257 | 0.5228 | 0.5088 | 0.4573 | 0.5247 | 0.4534 | 0.539 | 0.547 | 0.3922 | 0.3769 | 0.5858 | 0.4723 | 0.6368 | 0.5444 | 0.5598 | 0.3479 | 0.4993 |
Cytoplasmic Foci | 0.01 | 0.0126 | 0.0079 | 0.0124 | 0.0075 | 0.0112 | 0.0162 | 0.0084 | 0.0093 | 0.0099 | 0.0275 | 0.0107 | 0.0145 | 0.0079 | 0.0204 | 0.0066 | 0.0616 | 0.0083 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0166 | 0.0088 | 0.0106 | 0.012 | 0.007 | 0.0079 | 0.0095 | 0.0079 | 0.0136 | 0.0078 | 0.0027 | 0.006 | 0.0272 | 0.0084 | 0.0083 | 0.0162 | 0.003 | 0.0063 |
Endosome | 0.0097 | 0.0112 | 0.0071 | 0.0152 | 0.0123 | 0.0134 | 0.0125 | 0.006 | 0.0088 | 0.0073 | 0.0087 | 0.0057 | 0.0161 | 0.0037 | 0.0119 | 0.0174 | 0.0734 | 0.0083 |
Golgi | 0.0003 | 0.0006 | 0.0002 | 0.0002 | 0.0015 | 0.0003 | 0.0015 | 0.0002 | 0.001 | 0.0016 | 0.0019 | 0.0001 | 0.0043 | 0.0001 | 0.0021 | 0.0004 | 0.0407 | 0.0069 |
Lipid Particles | 0.0007 | 0.0008 | 0.0003 | 0.0006 | 0.0018 | 0.0006 | 0.002 | 0.0005 | 0.001 | 0.0012 | 0.0057 | 0.0001 | 0.0158 | 0.0004 | 0.002 | 0.0001 | 0.0041 | 0.01 |
Mitochondria | 0.0004 | 0.0008 | 0.0007 | 0.0008 | 0.0046 | 0.0005 | 0.0011 | 0.0009 | 0.0018 | 0.0017 | 0.0009 | 0.0008 | 0.0043 | 0.0001 | 0.0019 | 0.001 | 0.0154 | 0.0149 |
None | 0.3481 | 0.3451 | 0.3526 | 0.2713 | 0.3488 | 0.3167 | 0.3523 | 0.3466 | 0.3122 | 0.437 | 0.4086 | 0.2639 | 0.327 | 0.2974 | 0.3491 | 0.378 | 0.3608 | 0.4093 |
Nuclear Periphery | 0.0193 | 0.0145 | 0.0145 | 0.0286 | 0.0121 | 0.0123 | 0.0216 | 0.0104 | 0.0154 | 0.0159 | 0.0031 | 0.011 | 0.0216 | 0.0111 | 0.0146 | 0.0079 | 0.0052 | 0.0055 |
Nucleolus | 0.0003 | 0.0005 | 0.0003 | 0.0007 | 0.0035 | 0.0004 | 0.0013 | 0.0004 | 0.0009 | 0.0005 | 0.0003 | 0.0004 | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Nucleus | 0.0731 | 0.0525 | 0.0708 | 0.1292 | 0.1 | 0.0964 | 0.0884 | 0.0623 | 0.0545 | 0.076 | 0.0705 | 0.0967 | 0.0585 | 0.0291 | 0.0265 | 0.0075 | 0.0364 | 0.0147 |
Peroxisomes | 0.0001 | 0.0005 | 0.0002 | 0.0003 | 0.0026 | 0.0002 | 0.0022 | 0.0003 | 0.0005 | 0.002 | 0.0319 | 0.0002 | 0.0069 | 0 | 0.0021 | 0.0001 | 0.0341 | 0.0018 |
Punctate Nuclear | 0.0055 | 0.0088 | 0.0059 | 0.0133 | 0.0066 | 0.0098 | 0.014 | 0.0051 | 0.0139 | 0.0071 | 0.0295 | 0.0107 | 0.0075 | 0.0024 | 0.0086 | 0.0009 | 0.0018 | 0.0009 |
Vacuole | 0.003 | 0.0033 | 0.0037 | 0.0042 | 0.0034 | 0.0021 | 0.0032 | 0.0017 | 0.0019 | 0.0014 | 0.0008 | 0.0015 | 0.0025 | 0.0016 | 0.002 | 0.0016 | 0.0021 | 0.0011 |
Vacuole Periphery | 0.0007 | 0.0006 | 0.0006 | 0.001 | 0.0008 | 0.0005 | 0.0006 | 0.0003 | 0.0006 | 0.0005 | 0.0002 | 0.0004 | 0.0007 | 0.0002 | 0.0013 | 0.0008 | 0.001 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.2118 | 2.6623 | 4.9388 | 10.97 | 9.6533 | 10.4178 | 10.4309 | 15.897 | 13.9883 | 10.7667 |
Translational Efficiency | 2.3041 | 5.7792 | 2.3862 | 1.324 | 1.4096 | 1.9682 | 1.9655 | 1.2935 | 2.1908 | 1.9689 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1635 | 1571 | 2171 | 1346 | 2128 | 1515 | 192 | 1987 | 3763 | 3086 | 2363 | 3333 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 776.69 | 778.67 | 900.54 | 1205.31 | 774.43 | 788.12 | 1089.87 | 1094.48 | 775.41 | 783.31 | 915.92 | 1139.24 |
Standard Deviation | 102.86 | 128.05 | 124.95 | 403.84 | 98.97 | 126.19 | 218.04 | 222.84 | 100.69 | 127.23 | 144.51 | 313.72 |
Intensity Change Log 2 | — | 0.003673 | 0.213451 | 0.633993 | — | 0.025280 | 0.492949 | 0.499039 | — | 0.014502 | 0.359755 | 0.568192 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000178 | 0.000151 | 0.000246 | 0.003995 | 0.000083 | 0.000477 | 0.001401 | 0.000450 | 0.000124 | 0.000311 | 0.000340 | 0.001882 |
Bud Neck | 0.002621 | 0.004780 | 0.006190 | 0.012685 | 0.002444 | 0.013958 | 0.007480 | 0.008588 | 0.002521 | 0.009286 | 0.006295 | 0.010242 |
Bud Site | 0.002570 | 0.004373 | 0.004820 | 0.030638 | 0.001120 | 0.010712 | 0.003598 | 0.014000 | 0.001750 | 0.007485 | 0.004721 | 0.020719 |
Cell Periphery | 0.000103 | 0.000099 | 0.000090 | 0.000675 | 0.000056 | 0.000156 | 0.000201 | 0.000189 | 0.000077 | 0.000127 | 0.000099 | 0.000385 |
Cytoplasm | 0.465667* | 0.458170* | 0.231411* | 0.312966* | 0.507607* | 0.430331* | 0.064079 | 0.404821* | 0.489384* | 0.444503* | 0.217815* | 0.367726* |
Cytoplasmic Foci | 0.055378 | 0.029455 | 0.001666 | 0.007248 | 0.060484 | 0.092381 | 0.001157 | 0.005240 | 0.058265 | 0.060347 | 0.001625 | 0.006051 |
Eisosomes | 0.000036 | 0.000024 | 0.000016 | 0.003895 | 0.000025 | 0.000059 | 0.000042 | 0.000017 | 0.000030 | 0.000041 | 0.000018 | 0.001583 |
Endoplasmic Reticulum | 0.004116 | 0.002896 | 0.003994 | 0.004273 | 0.002746 | 0.002749 | 0.013937 | 0.003546 | 0.003341 | 0.002824 | 0.004802 | 0.003840 |
Endosome | 0.003960 | 0.002535 | 0.001115 | 0.002103 | 0.002456 | 0.008051 | 0.001690 | 0.003117 | 0.003110 | 0.005243 | 0.001162 | 0.002708 |
Golgi | 0.001162 | 0.000654 | 0.000059 | 0.002680 | 0.000660 | 0.004162 | 0.000473 | 0.001128 | 0.000878 | 0.002376 | 0.000093 | 0.001755 |
Lipid Particles | 0.005457 | 0.002290 | 0.000516 | 0.001371 | 0.003121 | 0.005908 | 0.001347 | 0.000771 | 0.004136 | 0.004066 | 0.000583 | 0.001014 |
Mitochondria | 0.002333 | 0.002458 | 0.001124 | 0.003089 | 0.001656 | 0.005222 | 0.001236 | 0.002417 | 0.001950 | 0.003815 | 0.001133 | 0.002688 |
Mitotic Spindle | 0.003430 | 0.004473 | 0.007510 | 0.057973 | 0.003213 | 0.015855 | 0.006345 | 0.035305 | 0.003307 | 0.010061 | 0.007415 | 0.044459 |
None | 0.007203 | 0.010836 | 0.007474 | 0.013212 | 0.004381 | 0.006026 | 0.004702 | 0.013442 | 0.005607 | 0.008475 | 0.007249 | 0.013349 |
Nuclear Periphery | 0.003826 | 0.002156 | 0.003358 | 0.002825 | 0.002890 | 0.001964 | 0.003135 | 0.003105 | 0.003297 | 0.002062 | 0.003340 | 0.002992 |
Nuclear Periphery Foci | 0.005258 | 0.001901 | 0.000727 | 0.002422 | 0.001637 | 0.002840 | 0.005780 | 0.001641 | 0.003210 | 0.002362 | 0.001137 | 0.001957 |
Nucleolus | 0.002417 | 0.001788 | 0.000630 | 0.000767 | 0.001051 | 0.002547 | 0.001134 | 0.000915 | 0.001644 | 0.002161 | 0.000671 | 0.000855 |
Nucleus | 0.409924* | 0.444861* | 0.708175* | 0.495445* | 0.382980* | 0.356413* | 0.845389* | 0.463674* | 0.394687* | 0.401440* | 0.719324* | 0.476504* |
Peroxisomes | 0.001287 | 0.001077 | 0.000292 | 0.000285 | 0.000638 | 0.005264 | 0.000306 | 0.000255 | 0.000920 | 0.003132 | 0.000293 | 0.000267 |
Vacuole | 0.022437 | 0.024160 | 0.020402 | 0.039486 | 0.020493 | 0.033649 | 0.035713 | 0.035945 | 0.021338 | 0.028819 | 0.021646 | 0.037375 |
Vacuole Periphery | 0.000639 | 0.000864 | 0.000185 | 0.001966 | 0.000260 | 0.001277 | 0.000854 | 0.001432 | 0.000425 | 0.001067 | 0.000240 | 0.001648 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.28 | -2.33 | -3.01 | -3.06 | -2.67 | -4.14 | -9.93 | -9.74 | -0.12 | 7.18 | -3.59 | -6.04 | -4.14 | -3.16 | -2.49 |
Bud Neck | -3.00 | -6.48 | -12.79 | -10.40 | -7.38 | -7.61 | -7.41 | -11.82 | 1.83 | -1.02 | -8.18 | -8.34 | -17.28 | -4.01 | -6.35 |
Bud Site | -1.44 | -1.72 | -7.08 | -6.10 | -6.28 | -7.05 | -5.65 | -7.80 | 0.78 | -3.95 | -6.45 | -3.57 | -10.22 | -4.01 | -6.84 |
Cell Periphery | 0.38 | 1.49 | -8.20 | -8.20 | -8.54 | -6.83 | -8.79 | -7.90 | 0.29 | 3.33 | -5.39 | -5.31 | -10.37 | -7.19 | -8.99 |
Cytoplasm | 1.59 | 33.89 | 28.10 | 25.38 | -2.78 | 8.84 | 54.02 | 27.47 | 14.66 | -28.86 | 7.67 | 50.81 | 38.40 | 27.10 | -13.16 |
Cytoplasmic Foci | 9.00 | 23.17 | 21.14 | 14.00 | -6.87 | -7.26 | 26.05 | 24.69 | 24.10 | -7.14 | -0.68 | 34.65 | 32.52 | 26.90 | -9.19 |
Eisosomes | 4.97 | 7.16 | -1.29 | -1.31 | -1.31 | -11.19 | -5.46 | 0.36 | 11.74 | 5.59 | -6.48 | 3.53 | -1.29 | -1.25 | -1.31 |
Endoplasmic Reticulum | 2.81 | -3.97 | -2.69 | -5.78 | 0.87 | -0.26 | -9.63 | -5.55 | -5.75 | 8.46 | 1.53 | -10.15 | -5.75 | -8.06 | 5.60 |
Endosome | 3.90 | 9.28 | 7.32 | 3.19 | -1.51 | -8.68 | 0.71 | 1.98 | 9.42 | 0.96 | -5.38 | 9.89 | 6.24 | 9.50 | -2.55 |
Golgi | 1.05 | 2.36 | 0.48 | -0.50 | -1.92 | -3.71 | 1.19 | 0.66 | 3.99 | -0.45 | -2.83 | 3.22 | 0.83 | 3.47 | -2.41 |
Lipid Particles | 5.65 | 10.64 | 7.67 | 1.36 | -6.50 | -5.40 | 4.06 | 10.14 | 10.17 | 2.32 | 0.18 | 14.67 | 11.76 | 9.09 | -6.80 |
Mitochondria | 0.02 | 1.93 | -0.54 | -0.52 | -6.07 | -4.14 | 0.26 | -1.38 | 3.80 | -3.46 | -3.11 | 1.78 | -1.34 | 2.68 | -6.90 |
Mitotic Spindle | -0.41 | -3.87 | -10.17 | -9.96 | -8.41 | -4.97 | -1.94 | -10.74 | -5.50 | -3.49 | -4.42 | -4.69 | -14.78 | -11.29 | -10.80 |
None | -2.85 | 0.42 | -5.71 | -0.80 | -6.64 | -3.22 | 0.62 | -13.64 | -10.17 | -10.52 | -4.27 | -2.05 | -13.92 | -5.87 | -10.15 |
Nuclear Periphery | 4.24 | -1.52 | -3.58 | -9.18 | -3.36 | 2.75 | -2.21 | -6.88 | -9.62 | -0.30 | 4.81 | -4.63 | -7.58 | -12.90 | -3.94 |
Nuclear Periphery Foci | 4.38 | 6.70 | 3.60 | -0.89 | -4.23 | -1.82 | -1.99 | -1.80 | 0.93 | 1.75 | 1.71 | 5.08 | 2.39 | 0.28 | -3.33 |
Nucleolus | 1.27 | 3.37 | 2.24 | 1.82 | -1.93 | -5.27 | -0.71 | -1.76 | 3.84 | -0.77 | -1.67 | 3.75 | 1.33 | 3.96 | -3.72 |
Nucleus | -4.12 | -36.26 | -15.07 | -10.95 | 16.27 | 2.76 | -26.21 | -18.56 | -18.66 | 16.23 | -1.50 | -50.51 | -23.73 | -20.29 | 25.28 |
Peroxisomes | 0.42 | 2.92 | 3.14 | 2.75 | 0.22 | -7.27 | 2.98 | 4.57 | 8.10 | 1.61 | -5.69 | 3.64 | 4.54 | 8.38 | 0.35 |
Vacuole | -2.51 | -5.16 | -15.03 | -13.43 | -11.85 | -6.52 | -5.29 | -15.70 | -9.51 | -1.21 | -6.62 | -7.25 | -21.70 | -16.46 | -13.85 |
Vacuole Periphery | -0.57 | 2.12 | -0.03 | 0.47 | -1.75 | -5.16 | -0.92 | -1.29 | 2.28 | -0.17 | -3.47 | 2.30 | -0.84 | 1.88 | -2.26 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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