Standard name
Human Ortholog
Description Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 0.68 0.52 0.42 0.42 0.44 0.33 0.32 0.28 0.3 0.28 0.27 0.76 0.62 0.61 0.46 0.47 0.47 0.62 0.62 0.57 0.64 0.55 0.54
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0.05 0.18 0.25 0.25 0.23 0.23 0.3 0.21 0 0 0 0 0 0 0 0 0 0.05 0.06 0
Nucleus 0 0 0 0 0.08 0.06 0.08 0.09 0.12 0.14 0.14 0.12 0.05 0 0 0 0 0 0.18 0.19 0.11 0.05 0.06 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.45 0.38 0.58 0.61 0.57 0.45 0.51 0.46 0.51 0.5 0.44 0.54 0.25 0.49 0.48 0.61 0.62 0.6 0.12 0.09 0.08 0.12 0.16 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 2 0 2 3
Bud 0 0 1 0 1 4 5 8 8 4 4 6 0 0 0 0 0 1 0 5 9 1 5 15
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 3 2 1 1 4
Bud Site 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 1 4 2 2 0 1 0 0 0 1 2 0 0 0 0 0 0 0
Cytoplasm 67 165 232 138 210 258 261 220 215 236 167 186 81 112 138 77 87 85 85 157 270 57 179 192
Endoplasmic Reticulum 1 2 2 0 3 2 2 2 3 4 2 2 4 0 3 2 2 4 0 0 26 1 11 10
Endosome 0 1 6 8 7 5 9 2 7 2 3 11 0 1 3 0 2 1 1 1 16 0 10 16
Golgi 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1 0 3 1 0 1 0 1 0
Mitochondria 3 5 12 22 23 106 199 171 174 178 181 142 0 0 0 3 3 1 1 3 4 4 20 11
Nucleus 4 6 8 13 42 36 67 65 89 107 81 85 5 5 0 2 5 6 25 49 53 4 19 16
Nuclear Periphery 0 0 0 0 3 3 5 8 4 2 2 10 0 0 0 1 0 0 2 1 31 1 6 6
Nucleolus 1 0 0 0 0 2 7 6 2 3 3 4 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 2 8 1 6 9
Vac/Vac Membrane 50 93 258 198 288 263 406 322 387 390 263 371 26 89 108 103 114 109 16 23 37 10 52 63
Unique Cell Count 111 243 444 326 503 583 791 694 755 785 596 687 106 182 227 169 184 181 139 256 478 89 327 359
Labelled Cell Count 127 272 522 379 578 680 962 808 893 929 706 820 116 207 252 191 215 210 139 256 478 89 327 359


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 5.9 6.6 6.1 6.6 6.2 6.7 6.7 7.6 7.5 7.9 8.2 6.1 7.0 7.6 8.8 8.8 8.6 – 6.4 7.1
Std Deviation (1e-4) 1.6 1.1 1.7 1.4 1.5 1.8 2.0 1.8 2.2 2.3 2.7 2.8 1.2 1.5 1.1 3.0 2.0 3.0 – 1.2 1.4
Intensity Change (Log2) – – – -0.11 -0.02 -0.09 0.01 0.02 0.2 0.18 0.25 0.3 -0.11 0.08 0.2 0.41 0.41 0.38 – -0.04 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.7 -3.2 -2.5 -6.6 -6.9 -8.2 -7.7 -7.9 -8.6 4.5 2.1 2.1 -1.5 -1.1 -1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 1.1 0.1 0 -0.3 0 0 0 0 0.3 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.7 1.5 7.7 10.1 9.8 9.4 9.3 11.4 8.6 0 0 0 0 0 0
Nucleus 1.8 4.5 3.4 4.7 5.1 6.1 6.8 6.7 6.3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.7 -0.3 -4.1 -2.3 -3.9 -2.3 -2.8 -4.5 -1.4 -6.2 -2.1 -2.6 0.6 0.9 0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2989 0.1636 0.0824 -0.2531 -0.2533 0.1898 1.4756 1.8449 1.6052 1.0876 1.4688 1.7665 0.2016 0.683 0.4367 0.2948 0.549 0.314
Actin 0.0063 0.0009 0.0006 0.0005 0.0146 0.0017 0.0067 0.0015 0.008 0.0332 0.0288 0.0014 0.017 0.0001 0.0029 0.0009 0.0085 0.0049
Bud 0.0001 0.0005 0.0002 0.0001 0.0015 0.0001 0.0004 0.0017 0.0011 0.0003 0.0003 0.0008 0.0002 0 0.0003 0.0001 0.0009 0.0006
Bud Neck 0.0007 0.0005 0.0003 0.0007 0.0009 0.001 0.005 0.0003 0.0007 0.0021 0.0008 0.0017 0.0009 0.0001 0.0003 0.0002 0.0004 0.0004
Bud Periphery 0.0001 0.0003 0.0001 0.0001 0.0038 0 0.0004 0.0013 0.0008 0.0004 0.0002 0.0016 0.0002 0 0.0006 0.0001 0.0006 0.005
Bud Site 0.0002 0.0112 0.0005 0.0002 0.0088 0.0001 0.0073 0.0047 0.0069 0.0013 0.0006 0.0002 0.0016 0.0004 0.0006 0.0002 0.0018 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0 0.0002 0 0.0002 0.0001 0.0001 0.0001 0 0 0.0002 0.0001 0.0001 0.0001 0 0.0001
Cytoplasm 0.5047 0.5257 0.5228 0.5088 0.4573 0.5247 0.4534 0.539 0.547 0.3922 0.3769 0.5858 0.4723 0.6368 0.5444 0.5598 0.3479 0.4993
Cytoplasmic Foci 0.01 0.0126 0.0079 0.0124 0.0075 0.0112 0.0162 0.0084 0.0093 0.0099 0.0275 0.0107 0.0145 0.0079 0.0204 0.0066 0.0616 0.0083
Eisosomes 0.0001 0.0001 0.0001 0 0.0004 0 0.0002 0.0001 0.0001 0.0004 0.0001 0 0.0002 0 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0166 0.0088 0.0106 0.012 0.007 0.0079 0.0095 0.0079 0.0136 0.0078 0.0027 0.006 0.0272 0.0084 0.0083 0.0162 0.003 0.0063
Endosome 0.0097 0.0112 0.0071 0.0152 0.0123 0.0134 0.0125 0.006 0.0088 0.0073 0.0087 0.0057 0.0161 0.0037 0.0119 0.0174 0.0734 0.0083
Golgi 0.0003 0.0006 0.0002 0.0002 0.0015 0.0003 0.0015 0.0002 0.001 0.0016 0.0019 0.0001 0.0043 0.0001 0.0021 0.0004 0.0407 0.0069
Lipid Particles 0.0007 0.0008 0.0003 0.0006 0.0018 0.0006 0.002 0.0005 0.001 0.0012 0.0057 0.0001 0.0158 0.0004 0.002 0.0001 0.0041 0.01
Mitochondria 0.0004 0.0008 0.0007 0.0008 0.0046 0.0005 0.0011 0.0009 0.0018 0.0017 0.0009 0.0008 0.0043 0.0001 0.0019 0.001 0.0154 0.0149
None 0.3481 0.3451 0.3526 0.2713 0.3488 0.3167 0.3523 0.3466 0.3122 0.437 0.4086 0.2639 0.327 0.2974 0.3491 0.378 0.3608 0.4093
Nuclear Periphery 0.0193 0.0145 0.0145 0.0286 0.0121 0.0123 0.0216 0.0104 0.0154 0.0159 0.0031 0.011 0.0216 0.0111 0.0146 0.0079 0.0052 0.0055
Nucleolus 0.0003 0.0005 0.0003 0.0007 0.0035 0.0004 0.0013 0.0004 0.0009 0.0005 0.0003 0.0004 0.0008 0.0001 0.0002 0.0001 0.0002 0.0001
Nucleus 0.0731 0.0525 0.0708 0.1292 0.1 0.0964 0.0884 0.0623 0.0545 0.076 0.0705 0.0967 0.0585 0.0291 0.0265 0.0075 0.0364 0.0147
Peroxisomes 0.0001 0.0005 0.0002 0.0003 0.0026 0.0002 0.0022 0.0003 0.0005 0.002 0.0319 0.0002 0.0069 0 0.0021 0.0001 0.0341 0.0018
Punctate Nuclear 0.0055 0.0088 0.0059 0.0133 0.0066 0.0098 0.014 0.0051 0.0139 0.0071 0.0295 0.0107 0.0075 0.0024 0.0086 0.0009 0.0018 0.0009
Vacuole 0.003 0.0033 0.0037 0.0042 0.0034 0.0021 0.0032 0.0017 0.0019 0.0014 0.0008 0.0015 0.0025 0.0016 0.002 0.0016 0.0021 0.0011
Vacuole Periphery 0.0007 0.0006 0.0006 0.001 0.0008 0.0005 0.0006 0.0003 0.0006 0.0005 0.0002 0.0004 0.0007 0.0002 0.0013 0.0008 0.001 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.2118 2.6623 4.9388 10.97 9.6533 10.4178 10.4309 15.897 13.9883 10.7667
Translational Efficiency 2.3041 5.7792 2.3862 1.324 1.4096 1.9682 1.9655 1.2935 2.1908 1.9689

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (43%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Opi10

Opi10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Opi10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available