Standard name
Description Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 0.68 0.52 0.42 0.42 0.44 0.33 0.32 0.28 0.3 0.28 0.27 0.76 0.62 0.61 0.46 0.47 0.47 0.62 0.62 0.57 0.64 0.55 0.54
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0.05 0.18 0.25 0.25 0.23 0.23 0.3 0.21 0 0 0 0 0 0 0 0 0 0.05 0.06 0
Nucleus 0 0 0 0 0.08 0.06 0.08 0.09 0.12 0.14 0.14 0.12 0.05 0 0 0 0 0 0.18 0.19 0.11 0.05 0.06 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.45 0.38 0.58 0.61 0.57 0.45 0.51 0.46 0.51 0.5 0.44 0.54 0.25 0.49 0.48 0.61 0.62 0.6 0.12 0.09 0.08 0.12 0.16 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 2 0 2 3
Bud 0 0 1 0 1 4 5 8 8 4 4 6 0 0 0 0 0 1 0 5 9 1 5 15
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 3 2 1 1 4
Bud Site 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1 0 0 0 1 4 2 2 0 1 0 0 0 1 2 0 0 0 0 0 0 0
Cytoplasm 67 165 232 138 210 258 261 220 215 236 167 186 81 112 138 77 87 85 85 157 270 57 179 192
Endoplasmic Reticulum 1 2 2 0 3 2 2 2 3 4 2 2 4 0 3 2 2 4 0 0 26 1 11 10
Endosome 0 1 6 8 7 5 9 2 7 2 3 11 0 1 3 0 2 1 1 1 16 0 10 16
Golgi 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1 0 3 1 0 1 0 1 0
Mitochondria 3 5 12 22 23 106 199 171 174 178 181 142 0 0 0 3 3 1 1 3 4 4 20 11
Nucleus 4 6 8 13 42 36 67 65 89 107 81 85 5 5 0 2 5 6 25 49 53 4 19 16
Nuclear Periphery 0 0 0 0 3 3 5 8 4 2 2 10 0 0 0 1 0 0 2 1 31 1 6 6
Nucleolus 1 0 0 0 0 2 7 6 2 3 3 4 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 2 8 1 6 9
Vac/Vac Membrane 50 93 258 198 288 263 406 322 387 390 263 371 26 89 108 103 114 109 16 23 37 10 52 63
Unique Cell Count 111 243 444 326 503 583 791 694 755 785 596 687 106 182 227 169 184 181 139 256 478 89 327 359
Labelled Cell Count 127 272 522 379 578 680 962 808 893 929 706 820 116 207 252 191 215 210 139 256 478 89 327 359


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 5.9 6.6 6.1 6.6 6.2 6.7 6.7 7.6 7.5 7.9 8.2 6.1 7.0 7.6 8.8 8.8 8.6 6.4 7.1
Std Deviation (1e-4) 1.6 1.1 1.7 1.4 1.5 1.8 2.0 1.8 2.2 2.3 2.7 2.8 1.2 1.5 1.1 3.0 2.0 3.0 1.2 1.4
Intensity Change (Log2) -0.11 -0.02 -0.09 0.01 0.02 0.2 0.18 0.25 0.3 -0.11 0.08 0.2 0.41 0.41 0.38 -0.04 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.7 -3.2 -2.5 -6.6 -6.9 -8.2 -7.7 -7.9 -8.6 4.5 2.1 2.1 -1.5 -1.1 -1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 1.1 0.1 0 -0.3 0 0 0 0 0.3 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.7 1.5 7.7 10.1 9.8 9.4 9.3 11.4 8.6 0 0 0 0 0 0
Nucleus 1.8 4.5 3.4 4.7 5.1 6.1 6.8 6.7 6.3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.7 -0.3 -4.1 -2.3 -3.9 -2.3 -2.8 -4.5 -1.4 -6.2 -2.1 -2.6 0.6 0.9 0.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Opi10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available