Standard name | |
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Description | Exosome core complex catalytic subunit; has both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; role in degradation of tRNAs; similar to E. coli RNase R and to human DIS3, which partially complements dis3-81 heat sensitivity; mutations in Dis3p analogous to human mutations implicated in multiple myeloma impair exosome function; protein abundance increases under to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Bud | 1 | 0 | 3 | 0 | 2 | 2 | 6 | 8 | 9 | 11 | 8 | 8 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 7 | 6 | 0 | 1 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 2 | 1 | 16 | 10 | 15 | 5 | 12 | 5 | 12 | 0 | 0 | 2 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 10 | 14 | 8 | 9 | 17 | 24 | 29 | 16 | 23 | 13 | 9 | 36 | 63 | 71 | 21 | 11 | 12 | 0 | 1 | 0 | 2 | 0 | 0 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 6 | 1 | 0 | 1 | 1 | 0 | 2 | 0 |
Endosome | 2 | 8 | 10 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 12 | 2 | 0 | 1 | 5 | 11 | 33 | 4 | 14 | 25 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 3 | 0 | 2 | 0 | 0 | 3 | 2 |
Mitochondria | 1 | 5 | 50 | 92 | 68 | 161 | 221 | 196 | 197 | 247 | 200 | 214 | 2 | 5 | 17 | 8 | 0 | 5 | 0 | 0 | 2 | 2 | 3 | 5 |
Nucleus | 65 | 103 | 240 | 252 | 335 | 326 | 402 | 499 | 272 | 318 | 276 | 271 | 146 | 189 | 209 | 214 | 99 | 131 | 49 | 108 | 155 | 69 | 80 | 68 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 4 | 2 | 8 | 2 | 3 | 0 | 9 | 7 | 0 | 2 | 1 | 3 | 0 | 1 | 1 | 0 | 6 | 0 | 2 | 6 |
Nucleolus | 46 | 106 | 114 | 37 | 10 | 23 | 47 | 94 | 24 | 47 | 39 | 59 | 47 | 66 | 66 | 36 | 18 | 29 | 87 | 156 | 202 | 61 | 111 | 114 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 1 | 7 | 2 | 0 | 1 | 3 | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 5 | 14 | 37 | 2 | 13 | 15 |
Vac/Vac Membrane | 17 | 43 | 25 | 13 | 49 | 48 | 43 | 45 | 13 | 25 | 13 | 15 | 17 | 27 | 27 | 39 | 21 | 28 | 5 | 17 | 30 | 34 | 92 | 95 |
Unique Cell Count | 122 | 246 | 403 | 327 | 429 | 464 | 614 | 735 | 392 | 513 | 427 | 417 | 233 | 335 | 370 | 306 | 146 | 194 | 159 | 326 | 483 | 184 | 331 | 345 |
Labelled Cell Count | 135 | 278 | 464 | 407 | 478 | 597 | 767 | 890 | 539 | 685 | 563 | 597 | 249 | 359 | 411 | 333 | 160 | 211 | 159 | 326 | 483 | 184 | 331 | 345 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 12.6 | 10.8 | 6.8 | 6.6 | 6.3 | 5.9 | 5.6 | 6.0 | 5.5 | 5.4 | 5.3 | 5.2 | 11.2 | 10.6 | 9.4 | 14.9 | 15.8 | 16.0 | 11.7 | 12.5 | 12.8 |
Std Deviation (1e-4) | 2.4 | 2.1 | 1.2 | 1.2 | 1.0 | 1.0 | 1.0 | 1.1 | 1.3 | 1.1 | 1.1 | 1.3 | 2.6 | 2.7 | 2.3 | 3.0 | 3.6 | 3.6 | 2.1 | 2.3 | 2.4 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.11 | -0.22 | -0.29 | -0.2 | -0.3 | -0.34 | -0.38 | -0.38 | 0.71 | 0.63 | 0.45 | 1.13 | 1.2 | 1.23 | 0.77 | 0.87 | 0.9 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1.8 | 1.7 | 1.4 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 3.8 | 0 | 2.9 | 0 | 3.1 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.8 | -1.2 | 0.2 | 0.4 | 0.4 | 0.4 | 0.8 | -0.3 | -1.1 | 5.4 | 6.8 | 7.0 | 2.1 | 2.0 | 1.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | -3.0 | -2.8 | 0 | 0 | 0 | 0 | 0 | 0 | -0.9 | 0.6 | -1.9 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.3 | 1.4 | 7.6 | 8.3 | 5.6 | 11.5 | 11.5 | 10.8 | 11.9 | -5.1 | -5.6 | -3.9 | -4.7 | -4.5 | -3.9 |
Nucleus | 5.0 | 5.8 | 3.3 | 1.9 | 2.8 | 2.9 | 0.7 | 1.5 | 1.6 | 0.8 | -0.9 | -0.9 | 2.9 | 1.8 | 1.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -5.6 | -10.5 | -9.4 | -8.8 | -6.5 | -8.2 | -7.5 | -7.1 | -5.0 | -2.3 | -2.7 | -3.4 | -5.3 | -3.9 | -3.6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.3 | 2.6 | 2.2 | 0.5 | -0.1 | -1.9 | -0.9 | -2.2 | -1.7 | 0.5 | 1.0 | 0.6 | 3.0 | 3.1 | 3.3 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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