Standard name
Description Exosome core complex catalytic subunit; has both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; role in degradation of tRNAs; similar to E. coli RNase R and to human DIS3, which partially complements dis3-81 heat sensitivity; mutations in Dis3p analogous to human mutations implicated in multiple myeloma impair exosome function; protein abundance increases under to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.19 0.19 0.07 0.08 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.12 0.28 0.16 0.35 0.36 0.27 0.5 0.48 0.47 0.51 0 0 0.05 0 0 0 0 0 0 0 0 0
Nucleus 0.53 0.42 0.6 0.77 0.78 0.7 0.65 0.68 0.69 0.62 0.65 0.65 0.63 0.56 0.56 0.7 0.68 0.68 0.31 0.33 0.32 0.38 0.24 0.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.38 0.43 0.28 0.11 0 0.05 0.08 0.13 0.06 0.09 0.09 0.14 0.2 0.2 0.18 0.12 0.12 0.15 0.55 0.48 0.42 0.33 0.34 0.33
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0
Vac/Vac Membrane 0.14 0.17 0.06 0 0.11 0.1 0.07 0.06 0 0.05 0 0 0.07 0.08 0.07 0.13 0.14 0.14 0 0.06 0.06 0.19 0.28 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 0
Bud 1 0 3 0 2 2 6 8 9 11 8 8 0 0 1 0 0 0 0 7 6 0 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud Site 0 0 0 2 1 16 10 15 5 12 5 12 0 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 10 14 8 9 17 24 29 16 23 13 9 36 63 71 21 11 12 0 1 0 2 0 0
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 5 6 1 0 1 1 0 2 0
Endosome 2 8 10 1 0 1 3 0 0 0 0 0 1 5 12 2 0 1 5 11 33 4 14 25
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 3 3 0 2 0 0 3 2
Mitochondria 1 5 50 92 68 161 221 196 197 247 200 214 2 5 17 8 0 5 0 0 2 2 3 5
Nucleus 65 103 240 252 335 326 402 499 272 318 276 271 146 189 209 214 99 131 49 108 155 69 80 68
Nuclear Periphery 0 1 1 0 4 2 8 2 3 0 9 7 0 2 1 3 0 1 1 0 6 0 2 6
Nucleolus 46 106 114 37 10 23 47 94 24 47 39 59 47 66 66 36 18 29 87 156 202 61 111 114
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0
SpindlePole 0 1 7 2 0 1 3 2 0 2 0 1 0 0 1 0 2 0 5 14 37 2 13 15
Vac/Vac Membrane 17 43 25 13 49 48 43 45 13 25 13 15 17 27 27 39 21 28 5 17 30 34 92 95
Unique Cell Count 122 246 403 327 429 464 614 735 392 513 427 417 233 335 370 306 146 194 159 326 483 184 331 345
Labelled Cell Count 135 278 464 407 478 597 767 890 539 685 563 597 249 359 411 333 160 211 159 326 483 184 331 345


Cytoplasm, Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.6 10.8 6.8 6.6 6.3 5.9 5.6 6.0 5.5 5.4 5.3 5.2 11.2 10.6 9.4 14.9 15.8 16.0 11.7 12.5 12.8
Std Deviation (1e-4) 2.4 2.1 1.2 1.2 1.0 1.0 1.0 1.1 1.3 1.1 1.1 1.3 2.6 2.7 2.3 3.0 3.6 3.6 2.1 2.3 2.4
Intensity Change (Log2) – – – -0.05 -0.11 -0.22 -0.29 -0.2 -0.3 -0.34 -0.38 -0.38 0.71 0.63 0.45 1.13 1.2 1.23 0.77 0.87 0.9


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1.8 1.7 1.4 1.5 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 3.8 0 2.9 0 3.1 0 3.4 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.8 -1.2 0.2 0.4 0.4 0.4 0.8 -0.3 -1.1 5.4 6.8 7.0 2.1 2.0 1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 -3.0 -2.8 0 0 0 0 0 0 -0.9 0.6 -1.9 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.3 1.4 7.6 8.3 5.6 11.5 11.5 10.8 11.9 -5.1 -5.6 -3.9 -4.7 -4.5 -3.9
Nucleus 5.0 5.8 3.3 1.9 2.8 2.9 0.7 1.5 1.6 0.8 -0.9 -0.9 2.9 1.8 1.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -5.6 -10.5 -9.4 -8.8 -6.5 -8.2 -7.5 -7.1 -5.0 -2.3 -2.7 -3.4 -5.3 -3.9 -3.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.3 2.6 2.2 0.5 -0.1 -1.9 -0.9 -2.2 -1.7 0.5 1.0 0.6 3.0 3.1 3.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dis3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available