ORF
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.13 0.15 0.21 0.15 0 0.05 – 0 0 0 0 0 0.22 0.16 0.21 – 0.11 – 0.09 0.13 0.15 0.11 0.23 0.23
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0.08 0.06 0.1 – 0 – 0 0 0 0 0.06 0.14
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 – 0 0.08 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0.05 0.05 0.05 0 0 – 0 0 0 0 0 0.1 0.11 0.12 – 0 – 0 0 0 0 0 0
Cytoplasm 0.06 0.12 0.05 0.12 0.11 0.15 – 0.14 0.25 0.26 0.32 0.3 0.16 0.41 0.54 – 0 – 0 0 0 0 0 0
Endoplasmic Reticulum 0.22 0.31 0.23 0.22 0.23 0.17 – 0.05 0.06 0 0.05 0 0.33 0.19 0.08 – 0.05 – 0.11 0.07 0.12 0.15 0.14 0.16
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 – 0 0.06 0 0 0 0
Golgi 0.05 0 0 0 0.06 0 – 0 0 0 0 0 0 0 0 – 0.05 – 0 0 0 0 0 0
Mitochondria 0 0.12 0 0 0 0 – 0 0 0 0.05 0 0 0 0 – 0 – 0 0 0 0.09 0.1 0.05
Nucleus 0.13 0.06 0.06 0.09 0 0.11 – 0.21 0.18 0.17 0.12 0.12 0.05 0.06 0 – 0.16 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0.05 – 0 0 0 0 0 0
Vac/Vac Membrane 0.56 0.25 0.5 0.47 0.63 0.61 – 0.71 0.66 0.65 0.64 0.65 0.26 0.16 0.1 – 0.74 – 0.65 0.5 0.62 0.47 0.33 0.3
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 18 10 47 33 11 17 – 3 7 8 2 8 77 50 73 – 2 – 14 15 42 7 32 40
Bud 1 2 5 9 0 0 – 3 2 0 2 7 26 20 34 – 0 – 5 4 2 2 8 24
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 1 3 – 0 – 5 8 4 1 4 6
Bud Site 2 0 1 0 1 0 – 1 0 0 0 0 0 2 3 – 0 – – – – – – –
Cell Periphery 6 3 12 11 6 8 – 1 8 7 4 3 33 33 41 – 0 – 1 0 5 2 4 1
Cytoplasm 8 8 12 26 35 56 – 99 152 151 161 161 54 126 187 – 0 – 0 3 1 1 1 2
Endoplasmic Reticulum 30 20 52 46 76 64 – 38 37 25 27 23 113 58 29 – 1 – 18 7 32 10 19 27
Endosome 0 1 2 3 0 0 – 1 3 2 0 4 7 2 1 – 0 – 0 7 1 0 2 5
Golgi 7 0 6 8 21 4 – 6 2 0 1 5 3 0 0 – 1 – 5 1 5 1 2 4
Mitochondria 6 8 9 4 10 4 – 17 19 19 26 10 2 0 0 – 0 – 4 4 7 6 13 8
Nucleus 18 4 14 19 9 39 – 145 110 97 62 66 17 17 8 – 3 – 0 0 0 0 0 0
Nuclear Periphery 2 1 1 0 2 2 – 2 7 1 0 2 0 1 0 – 0 – 0 1 0 0 1 1
Nucleolus 1 1 0 0 0 3 – 3 4 4 4 5 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 1 0 5 2 0 1 – 0 0 0 0 2 0 0 1 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 2 2 0 0 – 0 0 0 0 0 2 6 12 – 1 – 0 0 0 0 0 0
Vac/Vac Membrane 77 16 114 101 206 225 – 501 395 378 321 348 90 50 33 – 14 – 103 57 171 30 46 53
Unique Cell Count 138 65 228 213 329 370 704 601 581 501 532 345 308 344 19 160 115 278 66 139 179
Labelled Cell Count 177 74 282 264 377 423 820 746 692 610 644 424 366 425 22 160 115 278 66 139 179


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.5 7.6 11.2 12.4 16.5 15.8 – 15.5 15.6 14.7 14.3 14.6 8.7 7.4 6.8 – 10.8 – 12.3 12.3 12.2
Std Deviation (1e-4) 3.2 2.2 3.1 2.9 3.6 3.0 – 3.5 3.5 3.5 3.5 3.6 1.6 1.5 1.3 – 1.8 – 3.1 2.8 3.0
Intensity Change (Log2) – – – 0.15 0.56 0.5 – 0.47 0.47 0.39 0.35 0.38 -0.36 -0.6 -0.71 – -0.05 – 0.14 0.13 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.4 -6.6 -6.2 – -11.8 -10.1 -9.8 -10.1 -9.3 0.5 -1.3 0.2 – 0 –
Bud 1.2 0 0 – 0 0 0 0 0 2.8 2.3 3.6 – 0 –
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 – 0 –
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 – 0 –
Cell Periphery 0 -2.3 -2.0 – 0 -3.3 -3.4 -3.8 0 1.9 2.2 2.7 – 0 –
Cytoplasm 2.6 2.2 3.7 – 3.6 6.5 6.6 7.9 7.5 3.8 9.3 12.1 – 0 –
Endoplasmic Reticulum -0.3 0.1 -1.7 – -7.7 -6.9 -8.1 -7.0 -7.8 2.6 -1.1 -4.8 – 0 –
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 – 0 –
Golgi 0.7 2.0 0 – 0 0 0 0 0 0 0 0 – 0 –
Mitochondria -1.3 -0.6 0 – -1.2 -0.6 -0.5 0.7 -1.7 0 0 0 – 0 –
Nucleus 1.1 -2.0 1.8 – 5.0 4.4 3.9 2.6 2.6 -0.6 -0.3 -2.3 – 0 –
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 – 0 –
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 – 0 –
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 – 0 –
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 2.0 – 0 –
Vacuole -0.5 3.0 2.6 – 5.9 4.2 3.9 3.6 4.0 -5.9 -8.4 -10.8 – 2.0 –