Standard name | |
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Description | Ubiquitin-protein ligase involved in ER-associated degradation (ERAD) of misfolded proteins; upon autoubiquitination triggers retrotranslocation of misfolded proteins to cytosol for degradation; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 12 | 1 | 6 | 12 | 3 | 4 | 4 | 4 | 8 | 6 | 19 | 23 | 60 | 36 | 24 | – | 6 | 18 | 32 | 5 | 58 | 62 | 104 | 96 |
Bud | 2 | 2 | 11 | 8 | 6 | 12 | 14 | 19 | 23 | 20 | 19 | 20 | 11 | 9 | 5 | – | 3 | 4 | 4 | 0 | 10 | 8 | 13 | 10 |
Bud Neck | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 26 | 32 | 19 | – | 6 | 1 | 7 | 3 | 12 | 11 | 22 | 19 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 0 | 10 | 11 | 27 | 22 | 34 | 21 | 16 | 33 | 25 | 36 | 45 | 53 | 118 | – | 11 | 21 | 12 | 3 | 15 | 36 | 80 | 77 |
Cytoplasm | 176 | 38 | 188 | 128 | 173 | 184 | 175 | 198 | 127 | 146 | 146 | 137 | 721 | 900 | 940 | – | 72 | 50 | 107 | 26 | 69 | 59 | 126 | 149 |
Endoplasmic Reticulum | 29 | 9 | 9 | 20 | 21 | 40 | 54 | 49 | 48 | 56 | 90 | 94 | 110 | 96 | 78 | – | 3 | 9 | 40 | 6 | 37 | 59 | 89 | 87 |
Endosome | 0 | 0 | 7 | 2 | 35 | 26 | 24 | 38 | 15 | 13 | 10 | 18 | 1 | 1 | 3 | – | 0 | 2 | 4 | 2 | 6 | 3 | 12 | 6 |
Golgi | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 6 | 1 | 3 | 2 |
Mitochondria | 8 | 7 | 28 | 31 | 51 | 155 | 203 | 300 | 263 | 296 | 269 | 267 | 1 | 1 | 1 | – | 11 | 5 | 43 | 12 | 39 | 66 | 99 | 100 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 2 | 0 | 1 | 1 | 0 | 4 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 2 | 0 | 3 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 10 | 5 | 23 | 6 | 6 | 4 | 1 | 5 | 0 | 4 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 1 |
SpindlePole | 1 | 0 | 2 | 0 | 3 | 1 | 1 | 3 | 1 | 2 | 0 | 1 | 4 | 2 | 3 | – | 2 | 2 | 0 | 0 | 2 | 1 | 5 | 2 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 17 | 8 | 20 | 22 | 31 | 24 | 23 | 29 | 2 | 7 | 27 | – | 0 | 0 | 9 | 3 | 6 | 7 | 18 | 24 |
Unique Cell Count | 223 | 52 | 243 | 177 | 285 | 362 | 441 | 539 | 419 | 460 | 467 | 498 | 891 | 1050 | 1124 | 96 | 97 | 268 | 68 | 276 | 324 | 585 | 587 | |
Labelled Cell Count | 242 | 57 | 274 | 219 | 359 | 459 | 543 | 665 | 536 | 603 | 605 | 632 | 982 | 1138 | 1223 | 114 | 113 | 268 | 68 | 276 | 324 | 585 | 587 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 3.7 | 4.5 | 4.6 | 5.0 | 4.8 | 4.7 | 4.9 | 4.8 | 4.7 | 4.8 | 5.2 | 5.8 | 6.1 | 6.4 | 4.6 | 5.1 | 6.4 | 6.0 | 6.0 | 5.9 |
Std Deviation (1e-4) | 0.8 | 0.9 | 1.9 | 1.5 | 1.3 | 1.9 | 1.4 | 1.4 | 1.5 | 1.3 | 1.1 | 1.2 | 1.0 | 1.1 | 1.1 | 0.8 | 0.7 | 1.1 | 1.5 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.15 | 0.09 | 0.07 | 0.13 | 0.08 | 0.06 | 0.08 | 0.2 | 0.37 | 0.44 | 0.5 | 0.02 | 0.17 | 0.5 | 0.4 | 0.4 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |