Standard name
Description Ubiquitin-protein ligase involved in ER-associated degradation (ERAD) of misfolded proteins; upon autoubiquitination triggers retrotranslocation of misfolded proteins to cytosol for degradation; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 0 0.07 0 0 0 0 0 0 0 0.05 0.07 0 0 – 0.06 0.19 0.12 0.08 0.21 0.19 0.18 0.16
Bud 0 0 0.05 0.05 0 0 0 0 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0.05 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0.06 0.09 0.06 0.08 0 0 0.07 0.05 0.07 0.05 0.05 0.1 – 0.11 0.22 0.05 0.06 0.06 0.11 0.14 0.13
Cytoplasm 0.79 0.73 0.77 0.72 0.61 0.51 0.4 0.37 0.3 0.32 0.31 0.28 0.81 0.86 0.84 – 0.75 0.52 0.4 0.39 0.25 0.18 0.22 0.26
Endoplasmic Reticulum 0.13 0.17 0 0.11 0.07 0.11 0.12 0.09 0.11 0.12 0.19 0.19 0.12 0.09 0.07 – 0 0.09 0.15 0.1 0.13 0.18 0.15 0.15
Endosome 0 0 0 0 0.12 0.07 0.05 0.07 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0.13 0.12 0.18 0.18 0.43 0.46 0.56 0.63 0.64 0.58 0.54 0 0 0 – 0.11 0.05 0.16 0.18 0.14 0.2 0.17 0.17
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0.05 0 0.07 0.05 0.05 0.06 0 0 0 – 0 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 12 1 6 12 3 4 4 4 8 6 19 23 60 36 24 – 6 18 32 5 58 62 104 96
Bud 2 2 11 8 6 12 14 19 23 20 19 20 11 9 5 – 3 4 4 0 10 8 13 10
Bud Neck 5 0 0 0 0 0 0 1 0 0 0 0 26 32 19 – 6 1 7 3 12 11 22 19
Bud Site 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 8 0 10 11 27 22 34 21 16 33 25 36 45 53 118 – 11 21 12 3 15 36 80 77
Cytoplasm 176 38 188 128 173 184 175 198 127 146 146 137 721 900 940 – 72 50 107 26 69 59 126 149
Endoplasmic Reticulum 29 9 9 20 21 40 54 49 48 56 90 94 110 96 78 – 3 9 40 6 37 59 89 87
Endosome 0 0 7 2 35 26 24 38 15 13 10 18 1 1 3 – 0 2 4 2 6 3 12 6
Golgi 1 0 1 0 0 0 2 0 0 0 0 1 0 1 0 – 0 0 0 0 6 1 3 2
Mitochondria 8 7 28 31 51 155 203 300 263 296 269 267 1 1 1 – 11 5 43 12 39 66 99 100
Nucleus 0 0 0 0 0 0 3 1 1 2 0 1 1 0 4 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 1 3 0 2 0 3 1 0 0 1 – 0 0 0 0 1 0 0 0
Nucleolus 0 0 1 1 0 0 0 4 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 10 5 23 6 6 4 1 5 0 4 0 0 0 – 0 1 0 0 2 0 1 1
SpindlePole 1 0 2 0 3 1 1 3 1 2 0 1 4 2 3 – 2 2 0 0 2 1 5 2
Vac/Vac Membrane 0 0 1 0 17 8 20 22 31 24 23 29 2 7 27 – 0 0 9 3 6 7 18 24
Unique Cell Count 223 52 243 177 285 362 441 539 419 460 467 498 891 1050 1124 96 97 268 68 276 324 585 587
Labelled Cell Count 242 57 274 219 359 459 543 665 536 603 605 632 982 1138 1223 114 113 268 68 276 324 585 587


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 3.7 4.5 4.6 5.0 4.8 4.7 4.9 4.8 4.7 4.8 5.2 5.8 6.1 6.4 4.6 5.1 6.4 6.0 6.0 5.9
Std Deviation (1e-4) 0.8 0.9 1.9 1.5 1.3 1.9 1.4 1.4 1.5 1.3 1.1 1.2 1.0 1.1 1.1 0.8 0.7 1.1 1.5 1.3 1.4
Intensity Change (Log2) – – – 0.01 0.15 0.09 0.07 0.13 0.08 0.06 0.08 0.2 0.37 0.44 0.5 0.02 0.17 0.5 0.4 0.4 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –