Standard name | |
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Human Ortholog | |
Description | Ubiquitin-protein ligase involved in ER-associated degradation (ERAD) of misfolded proteins; upon autoubiquitination triggers retrotranslocation of misfolded proteins to cytosol for degradation; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 12 | 1 | 6 | 12 | 3 | 4 | 4 | 4 | 8 | 6 | 19 | 23 | 60 | 36 | 24 | – | 6 | 18 | 32 | 5 | 58 | 62 | 104 | 96 |
Bud | 2 | 2 | 11 | 8 | 6 | 12 | 14 | 19 | 23 | 20 | 19 | 20 | 11 | 9 | 5 | – | 3 | 4 | 4 | 0 | 10 | 8 | 13 | 10 |
Bud Neck | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 26 | 32 | 19 | – | 6 | 1 | 7 | 3 | 12 | 11 | 22 | 19 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 0 | 10 | 11 | 27 | 22 | 34 | 21 | 16 | 33 | 25 | 36 | 45 | 53 | 118 | – | 11 | 21 | 12 | 3 | 15 | 36 | 80 | 77 |
Cytoplasm | 176 | 38 | 188 | 128 | 173 | 184 | 175 | 198 | 127 | 146 | 146 | 137 | 721 | 900 | 940 | – | 72 | 50 | 107 | 26 | 69 | 59 | 126 | 149 |
Endoplasmic Reticulum | 29 | 9 | 9 | 20 | 21 | 40 | 54 | 49 | 48 | 56 | 90 | 94 | 110 | 96 | 78 | – | 3 | 9 | 40 | 6 | 37 | 59 | 89 | 87 |
Endosome | 0 | 0 | 7 | 2 | 35 | 26 | 24 | 38 | 15 | 13 | 10 | 18 | 1 | 1 | 3 | – | 0 | 2 | 4 | 2 | 6 | 3 | 12 | 6 |
Golgi | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 6 | 1 | 3 | 2 |
Mitochondria | 8 | 7 | 28 | 31 | 51 | 155 | 203 | 300 | 263 | 296 | 269 | 267 | 1 | 1 | 1 | – | 11 | 5 | 43 | 12 | 39 | 66 | 99 | 100 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 2 | 0 | 1 | 1 | 0 | 4 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 2 | 0 | 3 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 10 | 5 | 23 | 6 | 6 | 4 | 1 | 5 | 0 | 4 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 1 |
SpindlePole | 1 | 0 | 2 | 0 | 3 | 1 | 1 | 3 | 1 | 2 | 0 | 1 | 4 | 2 | 3 | – | 2 | 2 | 0 | 0 | 2 | 1 | 5 | 2 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 17 | 8 | 20 | 22 | 31 | 24 | 23 | 29 | 2 | 7 | 27 | – | 0 | 0 | 9 | 3 | 6 | 7 | 18 | 24 |
Unique Cell Count | 223 | 52 | 243 | 177 | 285 | 362 | 441 | 539 | 419 | 460 | 467 | 498 | 891 | 1050 | 1124 | 96 | 97 | 268 | 68 | 276 | 324 | 585 | 587 | |
Labelled Cell Count | 242 | 57 | 274 | 219 | 359 | 459 | 543 | 665 | 536 | 603 | 605 | 632 | 982 | 1138 | 1223 | 114 | 113 | 268 | 68 | 276 | 324 | 585 | 587 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 3.7 | 4.5 | 4.6 | 5.0 | 4.8 | 4.7 | 4.9 | 4.8 | 4.7 | 4.8 | 5.2 | 5.8 | 6.1 | 6.4 | 4.6 | 5.1 | 6.4 | 6.0 | 6.0 | 5.9 |
Std Deviation (1e-4) | 0.8 | 0.9 | 1.9 | 1.5 | 1.3 | 1.9 | 1.4 | 1.4 | 1.5 | 1.3 | 1.1 | 1.2 | 1.0 | 1.1 | 1.1 | 0.8 | 0.7 | 1.1 | 1.5 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.15 | 0.09 | 0.07 | 0.13 | 0.08 | 0.06 | 0.08 | 0.2 | 0.37 | 0.44 | 0.5 | 0.02 | 0.17 | 0.5 | 0.4 | 0.4 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3214 | 1.5003 | 1.4426 | 1.5557 | 1.9129 | 1.5664 | 1.4467 | 2.5374 | 1.2678 | 1.7543 | 0.8617 | 1.4904 | 1.6734 | 1.4003 | 1.0054 | 1.3668 | 1.2738 | 1.3403 |
Actin | 0.0552 | 0.0051 | 0.0137 | 0.09 | 0.0558 | 0.0156 | 0.011 | 0.0212 | 0.0565 | 0.0341 | 0.0102 | 0.0247 | 0.0641 | 0.0156 | 0.0053 | 0.003 | 0.1124 | 0.0168 |
Bud | 0.0061 | 0.0043 | 0.0033 | 0.0065 | 0.0021 | 0.0005 | 0.0022 | 0.0002 | 0.0014 | 0.0008 | 0.0004 | 0.0005 | 0.0014 | 0.0023 | 0.0007 | 0.0004 | 0.0012 | 0.0002 |
Bud Neck | 0.0151 | 0.0017 | 0.0015 | 0.0108 | 0.0029 | 0.0276 | 0.004 | 0.007 | 0.0104 | 0.0125 | 0.0009 | 0.0109 | 0.0031 | 0.0019 | 0.0105 | 0.0008 | 0.0026 | 0.0082 |
Bud Periphery | 0.0113 | 0.01 | 0.009 | 0.0174 | 0.0068 | 0.0018 | 0.0056 | 0.0004 | 0.0052 | 0.0015 | 0.0023 | 0.0028 | 0.0041 | 0.0051 | 0.0076 | 0.0027 | 0.0046 | 0.0008 |
Bud Site | 0.0225 | 0.0599 | 0.0116 | 0.0451 | 0.0076 | 0.0016 | 0.0101 | 0.0579 | 0.0041 | 0.0031 | 0.0007 | 0.0003 | 0.0079 | 0.0337 | 0.0187 | 0.0008 | 0.0021 | 0.0004 |
Cell Periphery | 0.0071 | 0.0057 | 0.0048 | 0.0124 | 0.0111 | 0.0039 | 0.0086 | 0.0079 | 0.0085 | 0.0053 | 0.004 | 0.0048 | 0.0239 | 0.0194 | 0.0073 | 0.0093 | 0.0103 | 0.0074 |
Cytoplasm | 0.2564 | 0.3231 | 0.3594 | 0.159 | 0.1877 | 0.3896 | 0.3075 | 0.3416 | 0.401 | 0.3897 | 0.3376 | 0.4342 | 0.2612 | 0.431 | 0.4221 | 0.311 | 0.3442 | 0.4979 |
Cytoplasmic Foci | 0.0425 | 0.0282 | 0.0249 | 0.0304 | 0.0793 | 0.0348 | 0.1069 | 0.0163 | 0.0288 | 0.0329 | 0.0137 | 0.0349 | 0.0383 | 0.0267 | 0.0177 | 0.0435 | 0.0207 | 0.0149 |
Eisosomes | 0.0009 | 0.0006 | 0.0006 | 0.0021 | 0.0015 | 0.0004 | 0.0008 | 0.0018 | 0.0015 | 0.0016 | 0.0006 | 0.0007 | 0.0022 | 0.0008 | 0.0008 | 0.0012 | 0.0012 | 0.0006 |
Endoplasmic Reticulum | 0.0923 | 0.0741 | 0.0543 | 0.0412 | 0.0373 | 0.0607 | 0.0518 | 0.0608 | 0.0593 | 0.0902 | 0.0344 | 0.0837 | 0.1099 | 0.0773 | 0.1058 | 0.2725 | 0.0971 | 0.0969 |
Endosome | 0.0381 | 0.0217 | 0.0223 | 0.028 | 0.04 | 0.0185 | 0.0255 | 0.0288 | 0.0148 | 0.0181 | 0.0079 | 0.0178 | 0.0143 | 0.0083 | 0.012 | 0.0235 | 0.005 | 0.0097 |
Golgi | 0.0096 | 0.0049 | 0.0077 | 0.0179 | 0.0109 | 0.0043 | 0.0067 | 0.0031 | 0.009 | 0.0116 | 0.0129 | 0.0115 | 0.0083 | 0.014 | 0.0044 | 0.006 | 0.0116 | 0.0068 |
Lipid Particles | 0.0261 | 0.0078 | 0.0086 | 0.0083 | 0.0206 | 0.0077 | 0.0165 | 0.0092 | 0.0134 | 0.0074 | 0.0479 | 0.0074 | 0.0205 | 0.0265 | 0.0112 | 0.0238 | 0.0094 | 0.0094 |
Mitochondria | 0.0026 | 0.0026 | 0.0102 | 0.0113 | 0.007 | 0.0025 | 0.0312 | 0.0003 | 0.0043 | 0.0016 | 0.0006 | 0.0061 | 0.0026 | 0.0006 | 0.0024 | 0.0027 | 0.0011 | 0.0083 |
None | 0.3705 | 0.4358 | 0.4441 | 0.458 | 0.4488 | 0.4043 | 0.3447 | 0.423 | 0.372 | 0.3713 | 0.5223 | 0.3525 | 0.4085 | 0.3308 | 0.3635 | 0.2836 | 0.358 | 0.3146 |
Nuclear Periphery | 0.0065 | 0.0029 | 0.0039 | 0.0044 | 0.0093 | 0.007 | 0.0141 | 0.0024 | 0.0014 | 0.0012 | 0.0009 | 0.0014 | 0.0049 | 0.0011 | 0.0026 | 0.003 | 0.0007 | 0.0017 |
Nucleolus | 0.0009 | 0.0007 | 0.0006 | 0.0023 | 0.0016 | 0.0012 | 0.001 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.001 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Nucleus | 0.0082 | 0.0021 | 0.0021 | 0.0048 | 0.0041 | 0.0028 | 0.0026 | 0.0013 | 0.0009 | 0.0005 | 0.0006 | 0.0007 | 0.0035 | 0.0008 | 0.0011 | 0.0009 | 0.0003 | 0.0005 |
Peroxisomes | 0.0115 | 0.0011 | 0.0098 | 0.017 | 0.0433 | 0.0027 | 0.0308 | 0.0003 | 0.0025 | 0.0126 | 0.0005 | 0.0013 | 0.0098 | 0.0009 | 0.0003 | 0.0045 | 0.0149 | 0.0014 |
Punctate Nuclear | 0.0094 | 0.0018 | 0.0023 | 0.0195 | 0.0078 | 0.0099 | 0.0093 | 0.0009 | 0.001 | 0.0006 | 0.0004 | 0.0006 | 0.0029 | 0.0009 | 0.0006 | 0.0022 | 0.0016 | 0.0004 |
Vacuole | 0.0063 | 0.0055 | 0.0046 | 0.0119 | 0.0125 | 0.0021 | 0.0059 | 0.0147 | 0.0035 | 0.0029 | 0.001 | 0.0024 | 0.0067 | 0.0019 | 0.0047 | 0.0038 | 0.0007 | 0.0024 |
Vacuole Periphery | 0.0008 | 0.0005 | 0.0007 | 0.0017 | 0.0019 | 0.0005 | 0.0033 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0006 | 0.0009 | 0.0001 | 0.0005 | 0.0005 | 0.0001 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.9935 | 14.2953 | 11.0497 | 18.3476 | 12.0945 | 16.6485 | 15.3573 | 22.153 | 17.8229 | 17.1807 |
Translational Efficiency | 0.6279 | 0.5447 | 0.788 | 0.4516 | 0.4671 | 0.6115 | 0.5838 | 0.3616 | 0.6019 | 0.3913 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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