Standard name
Description MAPKKK acting in the protein kinase C signaling pathway; the kinase C signaling pathway controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p; MAPKKK is an acronym for mitogen-activated protein (MAP) kinase kinase kinase

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0.05 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.9 0.94 0.93 0.99 0.97 0.92 0.89 0.86 0.77 0.71 0.74 0.97 0.99 0.98 0.95 0.91 0.7 0.7 0.65 0.73 0.74 0.78
Endoplasmic Reticulum 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.1 0.06 0.23 0.24 0.29 0.45 0.52 0.51 0 0 0 0 0 0.06 0 0.17 0.06 0.05 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 3 2 0 0 0 0 0 0 0 1 1 0 0 6 26 12 13 6 8 17 15
Bud 0 3 3 2 4 8 13 10 7 14 12 2 3 14 4 8 1 5 0 2 7 13
Bud Neck 0 0 3 0 3 2 1 6 2 1 2 0 1 0 0 3 11 23 8 12 16 12
Bud Site 0 2 0 0 0 0 0 0 1 0 1 0 0 0 0 2
Cell Periphery 7 8 1 7 9 5 8 5 4 7 5 12 8 16 23 25 4 4 5 2 4 3
Cytoplasm 188 301 187 292 426 393 571 549 278 267 354 331 590 654 471 561 156 240 143 194 349 476
Endoplasmic Reticulum 12 3 2 0 0 1 1 0 3 0 0 3 0 1 19 32 12 7 2 2 4 19
Endosome 0 2 0 1 1 2 2 0 0 1 0 3 1 2 2 9 3 5 1 2 9 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 2 0 3 0 0
Mitochondria 3 11 12 30 25 99 155 186 160 194 247 1 4 18 2 10 13 14 38 16 22 29
Nucleus 0 0 0 0 0 0 1 3 1 2 2 1 0 0 4 3 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0
Nucleolus 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0 0 0 0 0 0
SpindlePole 1 1 1 0 1 2 9 6 4 1 5 2 2 0 7 13 0 0 2 0 3 4
Vac/Vac Membrane 0 3 1 1 1 1 3 3 2 1 1 1 1 2 7 8 3 18 8 11 20 14
Unique Cell Count 209 320 201 296 441 426 642 640 359 376 481 340 598 665 498 619 224 344 221 265 470 609
Labelled Cell Count 217 338 212 333 471 513 764 769 462 488 630 359 610 707 545 703 224 344 221 265 470 609


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 4.5 3.6 3.7 3.8 3.5 3.2 3.2 2.8 2.8 2.6 4.9 5.1 4.4 6.5 6.6 5.2 4.6 5.3
Std Deviation (1e-4) 0.6 0.9 0.5 1.0 0.9 1.1 1.0 0.8 0.9 0.8 1.0 0.9 1.0 0.9 1.1 1.2 1.4 1.2 1.4
Intensity Change (Log2) 0.02 0.05 -0.05 -0.2 -0.2 -0.38 -0.39 -0.49 0.44 0.49 0.28 0.83 0.86 0.52 0.32 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bck1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available