Standard name | |
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Human Ortholog | |
Description | MAPKKK acting in the protein kinase C signaling pathway; the kinase C signaling pathway controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p; MAPKKK is an acronym for mitogen-activated protein (MAP) kinase kinase kinase |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 6 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | – | 6 | 26 | 12 | 13 | 6 | 8 | 17 | 15 |
Bud | 0 | 3 | 3 | 2 | 4 | 8 | 13 | 10 | 7 | – | 14 | 12 | 2 | 3 | 14 | – | 4 | 8 | 1 | 5 | 0 | 2 | 7 | 13 |
Bud Neck | 0 | 0 | 3 | 0 | 3 | 2 | 1 | 6 | 2 | – | 1 | 2 | 0 | 1 | 0 | – | 0 | 3 | 11 | 23 | 8 | 12 | 16 | 12 |
Bud Site | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | – | 0 | 2 | – | – | – | – | – | – |
Cell Periphery | 7 | 8 | 1 | 7 | 9 | 5 | 8 | 5 | 4 | – | 7 | 5 | 12 | 8 | 16 | – | 23 | 25 | 4 | 4 | 5 | 2 | 4 | 3 |
Cytoplasm | 188 | 301 | 187 | 292 | 426 | 393 | 571 | 549 | 278 | – | 267 | 354 | 331 | 590 | 654 | – | 471 | 561 | 156 | 240 | 143 | 194 | 349 | 476 |
Endoplasmic Reticulum | 12 | 3 | 2 | 0 | 0 | 1 | 1 | 0 | 3 | – | 0 | 0 | 3 | 0 | 1 | – | 19 | 32 | 12 | 7 | 2 | 2 | 4 | 19 |
Endosome | 0 | 2 | 0 | 1 | 1 | 2 | 2 | 0 | 0 | – | 1 | 0 | 3 | 1 | 2 | – | 2 | 9 | 3 | 5 | 1 | 2 | 9 | 7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 2 | 0 | 3 | 0 | 0 |
Mitochondria | 3 | 11 | 12 | 30 | 25 | 99 | 155 | 186 | 160 | – | 194 | 247 | 1 | 4 | 18 | – | 2 | 10 | 13 | 14 | 38 | 16 | 22 | 29 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | – | 2 | 2 | 1 | 0 | 0 | – | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 1 | 1 | 0 | 1 | 2 | 9 | 6 | 4 | – | 1 | 5 | 2 | 2 | 0 | – | 7 | 13 | 0 | 0 | 2 | 0 | 3 | 4 |
Vac/Vac Membrane | 0 | 3 | 1 | 1 | 1 | 1 | 3 | 3 | 2 | – | 1 | 1 | 1 | 1 | 2 | – | 7 | 8 | 3 | 18 | 8 | 11 | 20 | 14 |
Unique Cell Count | 209 | 320 | 201 | 296 | 441 | 426 | 642 | 640 | 359 | 376 | 481 | 340 | 598 | 665 | 498 | 619 | 224 | 344 | 221 | 265 | 470 | 609 | ||
Labelled Cell Count | 217 | 338 | 212 | 333 | 471 | 513 | 764 | 769 | 462 | 488 | 630 | 359 | 610 | 707 | 545 | 703 | 224 | 344 | 221 | 265 | 470 | 609 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 4.5 | 3.6 | 3.7 | 3.8 | 3.5 | 3.2 | 3.2 | 2.8 | – | 2.8 | 2.6 | 4.9 | 5.1 | 4.4 | – | 6.5 | 6.6 | 5.2 | 4.6 | 5.3 |
Std Deviation (1e-4) | 0.6 | 0.9 | 0.5 | 1.0 | 0.9 | 1.1 | 1.0 | 0.8 | 0.9 | – | 0.8 | 1.0 | 0.9 | 1.0 | 0.9 | – | 1.1 | 1.2 | 1.4 | 1.2 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.05 | -0.05 | -0.2 | -0.2 | -0.38 | – | -0.39 | -0.49 | 0.44 | 0.49 | 0.28 | – | 0.83 | 0.86 | 0.52 | 0.32 | 0.55 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.66 | 3.3726 | 3.2044 | 2.9334 | 2.6605 | 3.2837 | 2.0515 | 1.9933 | 1.8616 | 1.725 | 1.3809 | 1.6704 | -0.0246 | 0.5056 | 0.2547 | 0.3723 | 0.4764 | 0.5085 |
Actin | 0.0236 | 0.0006 | 0.0134 | 0.0025 | 0.013 | 0.0106 | 0.012 | 0.0093 | 0.0152 | 0.0054 | 0.0063 | 0.0201 | 0.0181 | 0.0026 | 0.0107 | 0.001 | 0.0021 | 0.003 |
Bud | 0.003 | 0.0007 | 0.0028 | 0.0002 | 0.0014 | 0.0011 | 0.0089 | 0.0099 | 0.0034 | 0.0011 | 0.0004 | 0.0013 | 0.0001 | 0.0007 | 0.0018 | 0.0005 | 0.0104 | 0.0026 |
Bud Neck | 0.0098 | 0.0008 | 0.0095 | 0.0041 | 0.0073 | 0.1255 | 0.002 | 0.0007 | 0.002 | 0.0031 | 0.0003 | 0.1231 | 0.0016 | 0.001 | 0.002 | 0.0362 | 0.0058 | 0.069 |
Bud Periphery | 0.0053 | 0.0017 | 0.0109 | 0.0007 | 0.0033 | 0.0033 | 0.007 | 0.0048 | 0.0032 | 0.0024 | 0.0008 | 0.0015 | 0.0002 | 0.0009 | 0.0082 | 0.001 | 0.0162 | 0.0163 |
Bud Site | 0.0129 | 0.0198 | 0.0142 | 0.0007 | 0.0035 | 0.0016 | 0.0075 | 0.0429 | 0.0115 | 0.0051 | 0.0004 | 0.001 | 0.0009 | 0.0153 | 0.0085 | 0.0013 | 0.0025 | 0.0007 |
Cell Periphery | 0.0075 | 0.0133 | 0.0057 | 0.003 | 0.016 | 0.0046 | 0.0009 | 0.0018 | 0.0006 | 0.0007 | 0.0002 | 0.0003 | 0.0036 | 0.0011 | 0.0028 | 0.0008 | 0.0019 | 0.0008 |
Cytoplasm | 0.4254 | 0.596 | 0.5353 | 0.429 | 0.6086 | 0.5677 | 0.3824 | 0.7026 | 0.7018 | 0.633 | 0.449 | 0.6097 | 0.3587 | 0.5707 | 0.3423 | 0.3525 | 0.396 | 0.4085 |
Cytoplasmic Foci | 0.0235 | 0.0061 | 0.0174 | 0.0105 | 0.0122 | 0.0261 | 0.0448 | 0.0148 | 0.0122 | 0.0417 | 0.0923 | 0.0258 | 0.0134 | 0.0055 | 0.0113 | 0.0113 | 0.0048 | 0.0069 |
Eisosomes | 0.0009 | 0.0003 | 0.0007 | 0.0003 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0008 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0087 | 0.0068 | 0.0064 | 0.0032 | 0.0033 | 0.0027 | 0.0079 | 0.0033 | 0.0034 | 0.0046 | 0.0033 | 0.0055 | 0.0071 | 0.0017 | 0.0039 | 0.0023 | 0.0023 | 0.0024 |
Endosome | 0.0149 | 0.0017 | 0.0067 | 0.0066 | 0.0049 | 0.0026 | 0.0489 | 0.0031 | 0.0097 | 0.0218 | 0.0133 | 0.0096 | 0.0047 | 0.0007 | 0.0063 | 0.0022 | 0.0186 | 0.0013 |
Golgi | 0.0046 | 0.0004 | 0.0026 | 0.001 | 0.0021 | 0.0013 | 0.0084 | 0.0012 | 0.0056 | 0.0122 | 0.0046 | 0.0021 | 0.0028 | 0.0003 | 0.0037 | 0.0018 | 0.0141 | 0.0003 |
Lipid Particles | 0.0087 | 0.0014 | 0.0059 | 0.0025 | 0.0052 | 0.001 | 0.0305 | 0.0008 | 0.0025 | 0.008 | 0.0525 | 0.0018 | 0.0024 | 0.0001 | 0.0009 | 0.0003 | 0.0081 | 0.0004 |
Mitochondria | 0.0046 | 0.0003 | 0.0057 | 0.001 | 0.0019 | 0.0006 | 0.0288 | 0.0003 | 0.0051 | 0.0063 | 0.0063 | 0.012 | 0.0004 | 0.0002 | 0.0011 | 0.0009 | 0.0014 | 0.0003 |
None | 0.3965 | 0.3414 | 0.3396 | 0.5098 | 0.2859 | 0.2432 | 0.3216 | 0.1985 | 0.2063 | 0.215 | 0.3498 | 0.1771 | 0.5809 | 0.3973 | 0.5922 | 0.5839 | 0.5129 | 0.4857 |
Nuclear Periphery | 0.009 | 0.0006 | 0.001 | 0.0023 | 0.002 | 0.0004 | 0.032 | 0.0004 | 0.0023 | 0.0098 | 0.0011 | 0.0007 | 0.0009 | 0.0002 | 0.0005 | 0.0002 | 0.0005 | 0.0002 |
Nucleolus | 0.0011 | 0.0008 | 0.0013 | 0.0008 | 0.0013 | 0.0002 | 0.0031 | 0.0002 | 0.0002 | 0.0011 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0159 | 0.0014 | 0.0017 | 0.0019 | 0.0015 | 0.0008 | 0.017 | 0.0009 | 0.0091 | 0.0039 | 0.0006 | 0.0012 | 0.0005 | 0.0005 | 0.0006 | 0.0005 | 0.0003 | 0.0004 |
Peroxisomes | 0.0086 | 0.0001 | 0.0081 | 0.0012 | 0.0084 | 0.0035 | 0.0106 | 0.0011 | 0.0018 | 0.0116 | 0.0151 | 0.0026 | 0.0003 | 0.0001 | 0.0005 | 0.0018 | 0.0003 | 0.0001 |
Punctate Nuclear | 0.0056 | 0.0005 | 0.0025 | 0.0168 | 0.0153 | 0.0011 | 0.0134 | 0.0013 | 0.0015 | 0.0047 | 0.0023 | 0.0023 | 0.0007 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0002 |
Vacuole | 0.0084 | 0.0054 | 0.0073 | 0.0016 | 0.0022 | 0.0015 | 0.0075 | 0.0019 | 0.0018 | 0.0053 | 0.0008 | 0.0013 | 0.0017 | 0.0006 | 0.0016 | 0.001 | 0.0009 | 0.0005 |
Vacuole Periphery | 0.0016 | 0.0001 | 0.0013 | 0.0003 | 0.0003 | 0.0001 | 0.0044 | 0.0001 | 0.0006 | 0.0029 | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0004 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.04 | 8.67 | 10.8875 | 14.2765 | 12.7359 | 10.936 | 14.9475 | 11.6056 | 11.5222 | 16.2092 |
Translational Efficiency | 0.4545 | 0.4824 | 0.411 | 0.2972 | 0.2978 | 0.6538 | 0.3011 | 0.4314 | 0.446 | 0.4011 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |