Standard name
Human Ortholog
Description MAPKKK acting in the protein kinase C signaling pathway; the kinase C signaling pathway controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p; MAPKKK is an acronym for mitogen-activated protein (MAP) kinase kinase kinase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0.06 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0.05 0.07 0 0.05 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0
Cytoplasm 0.9 0.94 0.93 0.99 0.97 0.92 0.89 0.86 0.77 – 0.71 0.74 0.97 0.99 0.98 – 0.95 0.91 0.7 0.7 0.65 0.73 0.74 0.78
Endoplasmic Reticulum 0.06 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0.05 0.06 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.1 0.06 0.23 0.24 0.29 0.45 – 0.52 0.51 0 0 0 – 0 0 0.06 0 0.17 0.06 0.05 0.05
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 3 2 0 0 0 0 0 0 – 0 1 1 0 0 – 6 26 12 13 6 8 17 15
Bud 0 3 3 2 4 8 13 10 7 – 14 12 2 3 14 – 4 8 1 5 0 2 7 13
Bud Neck 0 0 3 0 3 2 1 6 2 – 1 2 0 1 0 – 0 3 11 23 8 12 16 12
Bud Site 0 2 0 0 0 0 0 0 1 – 0 1 0 0 0 – 0 2 – – – – – –
Cell Periphery 7 8 1 7 9 5 8 5 4 – 7 5 12 8 16 – 23 25 4 4 5 2 4 3
Cytoplasm 188 301 187 292 426 393 571 549 278 – 267 354 331 590 654 – 471 561 156 240 143 194 349 476
Endoplasmic Reticulum 12 3 2 0 0 1 1 0 3 – 0 0 3 0 1 – 19 32 12 7 2 2 4 19
Endosome 0 2 0 1 1 2 2 0 0 – 1 0 3 1 2 – 2 9 3 5 1 2 9 7
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 2 0 2 0 3 0 0
Mitochondria 3 11 12 30 25 99 155 186 160 – 194 247 1 4 18 – 2 10 13 14 38 16 22 29
Nucleus 0 0 0 0 0 0 1 3 1 – 2 2 1 0 0 – 4 3 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 2 0 1 0 0
Nucleolus 0 0 0 0 1 0 0 1 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 2 0 0 – 0 1 0 0 0 0 0 0
SpindlePole 1 1 1 0 1 2 9 6 4 – 1 5 2 2 0 – 7 13 0 0 2 0 3 4
Vac/Vac Membrane 0 3 1 1 1 1 3 3 2 – 1 1 1 1 2 – 7 8 3 18 8 11 20 14
Unique Cell Count 209 320 201 296 441 426 642 640 359 376 481 340 598 665 498 619 224 344 221 265 470 609
Labelled Cell Count 217 338 212 333 471 513 764 769 462 488 630 359 610 707 545 703 224 344 221 265 470 609


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 4.5 3.6 3.7 3.8 3.5 3.2 3.2 2.8 – 2.8 2.6 4.9 5.1 4.4 – 6.5 6.6 5.2 4.6 5.3
Std Deviation (1e-4) 0.6 0.9 0.5 1.0 0.9 1.1 1.0 0.8 0.9 – 0.8 1.0 0.9 1.0 0.9 – 1.1 1.2 1.4 1.2 1.4
Intensity Change (Log2) – – – 0.02 0.05 -0.05 -0.2 -0.2 -0.38 – -0.39 -0.49 0.44 0.49 0.28 – 0.83 0.86 0.52 0.32 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.66 3.3726 3.2044 2.9334 2.6605 3.2837 2.0515 1.9933 1.8616 1.725 1.3809 1.6704 -0.0246 0.5056 0.2547 0.3723 0.4764 0.5085
Actin 0.0236 0.0006 0.0134 0.0025 0.013 0.0106 0.012 0.0093 0.0152 0.0054 0.0063 0.0201 0.0181 0.0026 0.0107 0.001 0.0021 0.003
Bud 0.003 0.0007 0.0028 0.0002 0.0014 0.0011 0.0089 0.0099 0.0034 0.0011 0.0004 0.0013 0.0001 0.0007 0.0018 0.0005 0.0104 0.0026
Bud Neck 0.0098 0.0008 0.0095 0.0041 0.0073 0.1255 0.002 0.0007 0.002 0.0031 0.0003 0.1231 0.0016 0.001 0.002 0.0362 0.0058 0.069
Bud Periphery 0.0053 0.0017 0.0109 0.0007 0.0033 0.0033 0.007 0.0048 0.0032 0.0024 0.0008 0.0015 0.0002 0.0009 0.0082 0.001 0.0162 0.0163
Bud Site 0.0129 0.0198 0.0142 0.0007 0.0035 0.0016 0.0075 0.0429 0.0115 0.0051 0.0004 0.001 0.0009 0.0153 0.0085 0.0013 0.0025 0.0007
Cell Periphery 0.0075 0.0133 0.0057 0.003 0.016 0.0046 0.0009 0.0018 0.0006 0.0007 0.0002 0.0003 0.0036 0.0011 0.0028 0.0008 0.0019 0.0008
Cytoplasm 0.4254 0.596 0.5353 0.429 0.6086 0.5677 0.3824 0.7026 0.7018 0.633 0.449 0.6097 0.3587 0.5707 0.3423 0.3525 0.396 0.4085
Cytoplasmic Foci 0.0235 0.0061 0.0174 0.0105 0.0122 0.0261 0.0448 0.0148 0.0122 0.0417 0.0923 0.0258 0.0134 0.0055 0.0113 0.0113 0.0048 0.0069
Eisosomes 0.0009 0.0003 0.0007 0.0003 0.0005 0.0004 0.0003 0.0001 0.0003 0.0001 0.0002 0.0003 0.0008 0.0002 0.0004 0.0002 0.0001 0.0002
Endoplasmic Reticulum 0.0087 0.0068 0.0064 0.0032 0.0033 0.0027 0.0079 0.0033 0.0034 0.0046 0.0033 0.0055 0.0071 0.0017 0.0039 0.0023 0.0023 0.0024
Endosome 0.0149 0.0017 0.0067 0.0066 0.0049 0.0026 0.0489 0.0031 0.0097 0.0218 0.0133 0.0096 0.0047 0.0007 0.0063 0.0022 0.0186 0.0013
Golgi 0.0046 0.0004 0.0026 0.001 0.0021 0.0013 0.0084 0.0012 0.0056 0.0122 0.0046 0.0021 0.0028 0.0003 0.0037 0.0018 0.0141 0.0003
Lipid Particles 0.0087 0.0014 0.0059 0.0025 0.0052 0.001 0.0305 0.0008 0.0025 0.008 0.0525 0.0018 0.0024 0.0001 0.0009 0.0003 0.0081 0.0004
Mitochondria 0.0046 0.0003 0.0057 0.001 0.0019 0.0006 0.0288 0.0003 0.0051 0.0063 0.0063 0.012 0.0004 0.0002 0.0011 0.0009 0.0014 0.0003
None 0.3965 0.3414 0.3396 0.5098 0.2859 0.2432 0.3216 0.1985 0.2063 0.215 0.3498 0.1771 0.5809 0.3973 0.5922 0.5839 0.5129 0.4857
Nuclear Periphery 0.009 0.0006 0.001 0.0023 0.002 0.0004 0.032 0.0004 0.0023 0.0098 0.0011 0.0007 0.0009 0.0002 0.0005 0.0002 0.0005 0.0002
Nucleolus 0.0011 0.0008 0.0013 0.0008 0.0013 0.0002 0.0031 0.0002 0.0002 0.0011 0.0002 0.0005 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001
Nucleus 0.0159 0.0014 0.0017 0.0019 0.0015 0.0008 0.017 0.0009 0.0091 0.0039 0.0006 0.0012 0.0005 0.0005 0.0006 0.0005 0.0003 0.0004
Peroxisomes 0.0086 0.0001 0.0081 0.0012 0.0084 0.0035 0.0106 0.0011 0.0018 0.0116 0.0151 0.0026 0.0003 0.0001 0.0005 0.0018 0.0003 0.0001
Punctate Nuclear 0.0056 0.0005 0.0025 0.0168 0.0153 0.0011 0.0134 0.0013 0.0015 0.0047 0.0023 0.0023 0.0007 0.0002 0.0004 0.0002 0.0002 0.0002
Vacuole 0.0084 0.0054 0.0073 0.0016 0.0022 0.0015 0.0075 0.0019 0.0018 0.0053 0.0008 0.0013 0.0017 0.0006 0.0016 0.001 0.0009 0.0005
Vacuole Periphery 0.0016 0.0001 0.0013 0.0003 0.0003 0.0001 0.0044 0.0001 0.0006 0.0029 0.0004 0.0002 0.0002 0 0.0002 0.0001 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.04 8.67 10.8875 14.2765 12.7359 10.936 14.9475 11.6056 11.5222 16.2092
Translational Efficiency 0.4545 0.4824 0.411 0.2972 0.2978 0.6538 0.3011 0.4314 0.446 0.4011

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘