Standard name
Human Ortholog
Description Phospholipase B (lysophospholipase) involved in lipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro; PLB3 has a paralog, PLB1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.78 0.75 0.67 0.75 0.52 0.51 0.49 0.55 0.6 0.62 0.6 0.66 0.69 0.78 0.91 0.43 0.39 0.32 0.2 0.18 0.09 0.06 0.05 0.09
Endoplasmic Reticulum 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0.12 0.08 0 0.05 0 0 0.06 0 0.08 0.13 0.1 0.14
Endosome 0 0 0.11 0.11 0.21 0.16 0.19 0.08 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0.12 0.12 0.08 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0
Mitochondria 0 0.1 0.05 0.08 0.07 0.24 0.26 0.31 0.37 0.38 0.47 0.38 0 0 0 0 0 0 0.11 0.09 0.18 0.11 0.1 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.14 0.11 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.26 0.16 0.21 0.11 0.28 0.19 0.14 0.13 0.11 0.11 0.09 0.07 0.16 0.11 0.09 0.63 0.68 0.74 0.54 0.57 0.41 0.45 0.57 0.54
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 5 0 4 4 1 2 2 1 1 3 0 1 1 1 1 1 0 2 0 6 0 2 0
Bud 0 2 0 3 4 7 22 37 11 25 39 29 0 0 1 0 0 0 4 9 8 1 3 4
Bud Neck 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 1 2 2 1 1 0
Bud Site 0 0 0 0 0 2 0 4 3 1 0 1 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 2 0 2 3 3 4 1 1 3 2 1 1 1 1 0 0 0 1 0 0 0 0
Cytoplasm 183 241 159 200 222 233 307 464 274 398 324 382 22 91 164 131 72 87 51 70 26 7 13 24
Endoplasmic Reticulum 7 20 13 1 13 5 3 7 1 4 5 1 4 9 3 16 4 12 15 14 23 17 27 37
Endosome 5 0 27 30 88 71 123 65 21 20 10 14 1 0 2 4 9 6 6 13 33 15 22 14
Golgi 2 0 1 3 3 1 0 0 0 0 0 0 0 0 0 1 0 4 1 2 9 6 14 10
Mitochondria 2 33 13 22 30 109 166 266 168 243 252 217 0 1 0 8 4 5 28 35 51 14 28 28
Nucleus 1 1 0 0 0 3 4 13 2 4 2 1 1 1 0 61 26 30 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 2 3 1 1 0 2 0 1 0 0 0 0 2 0 0 1 1 0
Nucleolus 0 0 0 0 3 3 15 17 3 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 2 3 1 0 9 2 1 2 1 3 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 5 5 17 17 8 11 6 18 0 0 0 0 0 0 0 6 2 2 0 1
Vac/Vac Membrane 61 51 49 30 121 86 90 107 49 73 50 42 5 13 17 192 124 199 140 216 119 59 155 148
Unique Cell Count 236 322 237 268 426 457 632 849 459 637 540 575 32 116 181 304 183 269 259 381 290 132 274 275
Labelled Cell Count 261 354 266 298 496 529 764 1010 544 785 698 712 35 118 190 415 240 343 259 381 290 132 274 275


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.8 5.9 6.4 6.0 5.4 4.8 4.6 4.3 3.8 3.9 3.7 7.3 6.3 6.3 7.2 8.7 10.4 9.4 9.2 9.0
Std Deviation (1e-4) 1.6 1.3 2.3 2.9 2.3 2.4 1.9 1.7 1.6 1.3 1.3 1.1 1.6 1.3 1.3 2.2 3.0 3.1 2.2 2.2 2.1
Intensity Change (Log2) – – – 0.11 0.01 -0.13 -0.31 -0.38 -0.48 -0.65 -0.6 -0.68 0.3 0.08 0.09 0.28 0.55 0.81 0.66 0.63 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 9.9 9.7 12.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.8149 12.3093 9.6838 7.1204 7.105 10.5118 8.4255 10.7558 10.454 11.7547 7.699 11.0926 9.7667 13.0924 11.0621 8.795 9.6101 11.9068
Actin 0.0333 0.0019 0.0099 0.0112 0.0678 0.0088 0.0313 0.0016 0.0074 0.0044 0.0041 0.0009 0.0127 0.003 0.0054 0.0025 0.002 0.0018
Bud 0.0019 0.0021 0.0043 0.0018 0.0044 0.0017 0.0017 0.0021 0.0039 0.0084 0.0067 0.0043 0.0016 0.004 0.0038 0.0061 0.0084 0.0012
Bud Neck 0.0013 0.0011 0.0027 0.0016 0.0014 0.0095 0.0014 0.0014 0.0038 0.002 0.0058 0.0032 0.0023 0.0035 0.0032 0.0064 0.0021 0.0068
Bud Periphery 0.0029 0.0022 0.0056 0.0035 0.0043 0.0029 0.0027 0.0037 0.0058 0.0184 0.015 0.0076 0.0028 0.0063 0.0068 0.0265 0.0106 0.0025
Bud Site 0.0033 0.0041 0.0108 0.0019 0.0021 0.001 0.0055 0.0084 0.0086 0.0032 0.0026 0.0028 0.011 0.0101 0.0041 0.0027 0.0022 0.0007
Cell Periphery 0.0016 0.0017 0.0021 0.0019 0.001 0.0009 0.0027 0.0023 0.0079 0.0026 0.0013 0.0019 0.0035 0.006 0.0092 0.0042 0.0017 0.0015
Cytoplasm 0.0847 0.2617 0.1743 0.1346 0.0498 0.0978 0.1097 0.2109 0.2112 0.1296 0.1389 0.1131 0.1067 0.2273 0.2327 0.172 0.1777 0.1697
Cytoplasmic Foci 0.0279 0.0197 0.0251 0.0194 0.033 0.0199 0.055 0.0174 0.025 0.0136 0.045 0.0167 0.0295 0.018 0.0239 0.0262 0.0285 0.0233
Eisosomes 0.0003 0 0.0001 0.0001 0.0006 0.0001 0.0003 0 0.0001 0 0 0 0.0001 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0834 0.0638 0.0738 0.0472 0.0422 0.0707 0.1098 0.119 0.098 0.06 0.0428 0.1329 0.1338 0.1266 0.1247 0.1498 0.0455 0.1832
Endosome 0.2964 0.2721 0.2298 0.2684 0.2724 0.3372 0.3161 0.2292 0.219 0.2951 0.2359 0.3069 0.2957 0.1723 0.2041 0.2815 0.2869 0.2754
Golgi 0.0443 0.0289 0.0349 0.0253 0.0183 0.0384 0.0484 0.0234 0.0438 0.0392 0.0523 0.0481 0.0412 0.0239 0.0422 0.0389 0.0222 0.0364
Lipid Particles 0.0174 0.0067 0.0042 0.0045 0.0086 0.004 0.013 0.0043 0.0047 0.0009 0.0076 0.0077 0.0142 0.0117 0.0036 0.0025 0.0011 0.0039
Mitochondria 0.0597 0.0201 0.0279 0.042 0.0094 0.0272 0.0255 0.0581 0.0192 0.0713 0.0232 0.0244 0.0285 0.0084 0.0221 0.0263 0.0062 0.0241
None 0.0071 0.0005 0.0054 0.0008 0.002 0.0006 0.0043 0.0021 0.0013 0.0006 0.0008 0.0131 0.0114 0.0011 0.0024 0.0008 0.0004 0.0006
Nuclear Periphery 0.023 0.0117 0.019 0.0252 0.0181 0.0244 0.025 0.0183 0.0154 0.0119 0.0093 0.0215 0.0433 0.0279 0.022 0.0125 0.0121 0.024
Nucleolus 0.0006 0.0002 0.0003 0.0003 0.0004 0.0002 0.0004 0.0003 0.0006 0.0003 0.0004 0.0012 0.0015 0.001 0.0003 0.0003 0.0003 0.0001
Nucleus 0.004 0.0029 0.0032 0.0049 0.0027 0.003 0.0037 0.0032 0.0043 0.0027 0.003 0.004 0.0053 0.0049 0.0038 0.003 0.0025 0.0031
Peroxisomes 0.0079 0.0002 0.0049 0.0016 0.0121 0.001 0.0142 0.0002 0.0005 0.0003 0.0042 0.0002 0.0052 0.0004 0.0004 0.0005 0.0004 0.0005
Punctate Nuclear 0.0026 0.0002 0.0013 0.0005 0.0137 0.0002 0.0022 0.0004 0.0004 0.0001 0.0004 0.0005 0.0011 0.0005 0.0003 0.0003 0.0002 0.0003
Vacuole 0.2262 0.2406 0.2643 0.3342 0.3241 0.2585 0.1864 0.2465 0.2635 0.2165 0.31 0.2207 0.2003 0.2965 0.2378 0.1893 0.3202 0.1859
Vacuole Periphery 0.0701 0.0574 0.096 0.069 0.1117 0.0921 0.0406 0.0472 0.0557 0.1189 0.0907 0.0681 0.0484 0.0467 0.0469 0.0479 0.0688 0.0549

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.6979 14.8363 28.4108 37.9979 23.6006 29.4292 22.5015 23.8419 26.5102 31.5656
Translational Efficiency 0.6199 0.3316 0.3029 0.5832 0.6387 0.7522 0.194 0.1619 0.3899 0.28

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
320 509 132 1400 1847 1010 366 1799 2167 1519 498 3199

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1222.59 811.66 1170.52 1373.86 667.34 897.31 1306.28 1391.81 749.33 868.61 1270.30 1383.95
Standard Deviation 207.89 147.14 200.67 250.37 92.14 143.11 191.54 263.48 228.96 150.02 203.04 257.98
Intensity Change Log 2 — -0.590993 -0.062791 0.168294 — 0.427185 0.968970 1.060468 — -0.145206 0.390145 0.549296

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.018860 0.002879 0.002164 0.013516 0.000199 0.000342 0.011443 0.011361 0.002955 0.001192 0.008984 0.012304
Bud Neck 0.010077 0.030624 0.005987 0.005346 0.031718 0.013972 0.012562 0.004677 0.028522 0.019552 0.010819 0.004970
Bud Site 0.185837* 0.089496 0.040883 0.190047* 0.008725 0.042190 0.157769* 0.185899* 0.034879 0.058042 0.126787* 0.187714*
Cell Periphery 0.007185 0.000445 0.000311 0.003606 0.000168 0.001302 0.002546 0.002591 0.001205 0.001015 0.001953 0.003035
Cytoplasm 0.089581 0.153118* 0.207611* 0.091135 0.399979* 0.048875 0.110608* 0.135881* 0.354142* 0.083806 0.136320* 0.116299*
Cytoplasmic Foci 0.135135 0.261109* 0.158867 0.099519 0.296501* 0.107847 0.136501 0.114257 0.272672* 0.159204 0.142430 0.107807
Eisosomes 0.000353 0.000160 0.000042 0.000099 0.000231 0.000117 0.000141 0.000093 0.000249 0.000131 0.000114 0.000096
Endoplasmic Reticulum 0.033111 0.005311 0.014915 0.013944 0.001282 0.014961 0.067284 0.010069 0.005982 0.011728 0.053403 0.011765
Endosome 0.109845 0.135574 0.099343 0.090381 0.027247 0.168495 0.108296 0.086851 0.039444 0.157463 0.105923 0.088396
Golgi 0.066982 0.046134 0.025398 0.157570* 0.005287 0.042604 0.057272 0.159627* 0.014398 0.043787 0.048823 0.158727*
Lipid Particles 0.009147 0.006771 0.012151 0.011106 0.015604 0.013375 0.020882 0.007232 0.014650 0.011162 0.018568 0.008928
Mitochondria 0.003358 0.023856 0.000214 0.009786 0.011893 0.056111 0.001716 0.021287 0.010633 0.045303 0.001318 0.016253
Mitotic Spindle 0.007094 0.004310 0.010615 0.031825 0.002167 0.001168 0.022415 0.027115 0.002894 0.002221 0.019287 0.029177
None 0.000055 0.001773 0.000183 0.000226 0.002434 0.001174 0.000096 0.000199 0.002082 0.001375 0.000119 0.000211
Nuclear Periphery 0.000165 0.000272 0.000912 0.000557 0.000647 0.000734 0.001131 0.000229 0.000576 0.000579 0.001073 0.000373
Nuclear Periphery Foci 0.002519 0.001116 0.041127 0.005412 0.000702 0.000686 0.010392 0.001920 0.000971 0.000830 0.018539 0.003448
Nucleolus 0.000131 0.000936 0.000662 0.000172 0.001512 0.001852 0.000147 0.000108 0.001308 0.001545 0.000284 0.000136
Nucleus 0.001253 0.020657 0.017007 0.000917 0.112538 0.009333 0.003603 0.000750 0.096104 0.013128 0.007156 0.000823
Peroxisomes 0.006489 0.034001 0.003704 0.003529 0.010717 0.005592 0.005100 0.003563 0.010093 0.015112 0.004730 0.003548
Vacuole 0.268425* 0.153385 0.335072* 0.203519* 0.068414 0.353488* 0.246466* 0.162180 0.097949 0.286436* 0.269952* 0.180271
Vacuole Periphery 0.044398 0.028071 0.022831 0.067786 0.002037 0.115781* 0.023631 0.064110 0.008292 0.086390 0.023419 0.065719

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.45 3.62 1.09 -6.83 -7.52 -3.26 -5.03 -9.01 -8.89 0.04 2.36 -3.38 -8.29 -11.72 -1.74
Bud Neck -6.10 2.73 3.44 7.86 0.46 7.57 8.75 14.65 6.09 7.17 4.19 9.68 14.73 9.75 6.79
Bud Site 6.86 9.88 -0.41 -11.16 -14.98 -10.82 -15.04 -33.48* -23.92 -2.66 -6.03 -11.16 -33.22 -25.99 -7.05
Cell Periphery 2.61 2.66 1.36 -6.10 -6.44 -6.46 -4.75 -8.81 -3.87 -0.17 0.41 -1.38 -3.91 -6.72 -2.44
Cytoplasm -5.23 -5.23 -0.36 5.87 5.29 48.61* 25.20 32.52 -15.32 -2.38 37.23 19.31 34.01 -6.83 2.13
Cytoplasmic Foci -10.38 -1.51 4.15 16.89 4.53 27.52* 18.43 30.50 -0.92 2.78 16.98 16.95 31.63 10.45 5.16
Eisosomes 1.55 2.54 2.08 4.15 -5.46 8.70 3.67 11.36 2.13 1.94 5.59 5.18 7.63 4.04 1.06
Endoplasmic Reticulum 5.08 2.98 3.43 -4.95 0.22 -9.55 -8.21 -7.45 2.68 7.01 -4.42 -7.77 -4.89 -0.05 6.77
Endosome -2.81 1.13 2.96 6.11 0.93 -26.37* -14.18 -22.46 14.82 3.48 -26.23 -13.46 -20.76 15.62 3.43
Golgi 2.46 4.56 -9.97 -15.35 -15.85 -13.24 -8.88 -31.15* -20.27 -13.73 -11.32 -7.13 -36.84 -25.60 -18.84
Lipid Particles 1.26 -1.33 -1.26 -2.69 0.33 1.06 -1.95 6.74 2.80 4.62 2.26 -1.90 5.01 1.16 4.23
Mitochondria -6.68 3.29 -4.75 4.65 -10.10 -11.24 8.69 -4.46 8.65 -11.52 -12.00 9.30 -3.71 10.18 -14.10
Mitotic Spindle 1.29 -0.68 -6.23 -9.11 -4.90 1.48 -5.27 -10.83 -11.61 -1.23 1.11 -5.43 -13.71 -14.86 -2.94
None -2.66 -2.43 -4.83 2.40 -0.69 3.13 8.33 7.98 3.33 -4.78 1.97 8.18 7.82 4.01 -3.68
Nuclear Periphery -1.57 -5.01 -3.78 -2.69 1.90 -0.45 -1.16 2.54 3.63 2.22 -0.04 -1.56 1.32 1.88 2.28
Nuclear Periphery Foci 1.72 -4.88 -2.75 -5.25 4.50 0.04 -5.88 -2.85 -5.84 5.18 0.38 -7.10 -5.05 -7.35 6.04
Nucleolus -5.76 -2.06 -0.69 5.11 1.85 -0.54 7.65 7.86 5.31 1.33 -0.45 6.36 7.62 6.29 1.91
Nucleus -6.50 -3.32 0.86 6.58 3.38 28.20 31.22 33.13 5.98 4.25 25.01 26.73 31.74 8.81 4.76
Peroxisomes -7.01 2.28 3.57 7.91 0.10 5.73 6.15 9.41 3.32 2.26 -3.28 6.81 10.10 8.30 2.03
Vacuole 6.67 -2.26 3.88 -4.93 5.51 -29.13 -14.07 -18.02 17.88 6.34 -22.94 -14.93 -16.25 12.30 7.56
Vacuole Periphery 2.92 3.63 -4.17 -9.13 -8.97 -26.69* -8.07 -24.60* 10.84 -11.27 -23.96 -6.33 -27.30 6.05 -14.51
Standard name
Human Ortholog
Description Phospholipase B (lysophospholipase) involved in lipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro; PLB3 has a paralog, PLB1, that arose from the whole genome duplication
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Plb3

Plb3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Plb3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available