Standard name
Description Phospholipase B (lysophospholipase) involved in lipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro; PLB3 has a paralog, PLB1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.78 0.75 0.67 0.75 0.52 0.51 0.49 0.55 0.6 0.62 0.6 0.66 0.69 0.78 0.91 0.43 0.39 0.32 0.2 0.18 0.09 0.06 0.05 0.09
Endoplasmic Reticulum 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0.12 0.08 0 0.05 0 0 0.06 0 0.08 0.13 0.1 0.14
Endosome 0 0 0.11 0.11 0.21 0.16 0.19 0.08 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0.12 0.12 0.08 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0
Mitochondria 0 0.1 0.05 0.08 0.07 0.24 0.26 0.31 0.37 0.38 0.47 0.38 0 0 0 0 0 0 0.11 0.09 0.18 0.11 0.1 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.14 0.11 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.26 0.16 0.21 0.11 0.28 0.19 0.14 0.13 0.11 0.11 0.09 0.07 0.16 0.11 0.09 0.63 0.68 0.74 0.54 0.57 0.41 0.45 0.57 0.54
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 5 0 4 4 1 2 2 1 1 3 0 1 1 1 1 1 0 2 0 6 0 2 0
Bud 0 2 0 3 4 7 22 37 11 25 39 29 0 0 1 0 0 0 4 9 8 1 3 4
Bud Neck 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 1 2 2 1 1 0
Bud Site 0 0 0 0 0 2 0 4 3 1 0 1 0 0 1 0 0 0
Cell Periphery 0 0 2 0 2 3 3 4 1 1 3 2 1 1 1 1 0 0 0 1 0 0 0 0
Cytoplasm 183 241 159 200 222 233 307 464 274 398 324 382 22 91 164 131 72 87 51 70 26 7 13 24
Endoplasmic Reticulum 7 20 13 1 13 5 3 7 1 4 5 1 4 9 3 16 4 12 15 14 23 17 27 37
Endosome 5 0 27 30 88 71 123 65 21 20 10 14 1 0 2 4 9 6 6 13 33 15 22 14
Golgi 2 0 1 3 3 1 0 0 0 0 0 0 0 0 0 1 0 4 1 2 9 6 14 10
Mitochondria 2 33 13 22 30 109 166 266 168 243 252 217 0 1 0 8 4 5 28 35 51 14 28 28
Nucleus 1 1 0 0 0 3 4 13 2 4 2 1 1 1 0 61 26 30 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 2 3 1 1 0 2 0 1 0 0 0 0 2 0 0 1 1 0
Nucleolus 0 0 0 0 3 3 15 17 3 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 2 3 1 0 9 2 1 2 1 3 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 5 5 17 17 8 11 6 18 0 0 0 0 0 0 0 6 2 2 0 1
Vac/Vac Membrane 61 51 49 30 121 86 90 107 49 73 50 42 5 13 17 192 124 199 140 216 119 59 155 148
Unique Cell Count 236 322 237 268 426 457 632 849 459 637 540 575 32 116 181 304 183 269 259 381 290 132 274 275
Labelled Cell Count 261 354 266 298 496 529 764 1010 544 785 698 712 35 118 190 415 240 343 259 381 290 132 274 275


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.8 5.9 6.4 6.0 5.4 4.8 4.6 4.3 3.8 3.9 3.7 7.3 6.3 6.3 7.2 8.7 10.4 9.4 9.2 9.0
Std Deviation (1e-4) 1.6 1.3 2.3 2.9 2.3 2.4 1.9 1.7 1.6 1.3 1.3 1.1 1.6 1.3 1.3 2.2 3.0 3.1 2.2 2.2 2.1
Intensity Change (Log2) 0.11 0.01 -0.13 -0.31 -0.38 -0.48 -0.65 -0.6 -0.68 0.3 0.08 0.09 0.28 0.55 0.81 0.66 0.63 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 9.9 9.7 12.0