Standard name | |
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Description | Phospholipase B (lysophospholipase) involved in lipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro; PLB3 has a paralog, PLB1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 5 | 0 | 4 | 4 | 1 | 2 | 2 | 1 | 1 | 3 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 2 | 0 | 6 | 0 | 2 | 0 |
Bud | 0 | 2 | 0 | 3 | 4 | 7 | 22 | 37 | 11 | 25 | 39 | 29 | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 9 | 8 | 1 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 4 | 3 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 0 | 2 | 3 | 3 | 4 | 1 | 1 | 3 | 2 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 183 | 241 | 159 | 200 | 222 | 233 | 307 | 464 | 274 | 398 | 324 | 382 | 22 | 91 | 164 | 131 | 72 | 87 | 51 | 70 | 26 | 7 | 13 | 24 |
Endoplasmic Reticulum | 7 | 20 | 13 | 1 | 13 | 5 | 3 | 7 | 1 | 4 | 5 | 1 | 4 | 9 | 3 | 16 | 4 | 12 | 15 | 14 | 23 | 17 | 27 | 37 |
Endosome | 5 | 0 | 27 | 30 | 88 | 71 | 123 | 65 | 21 | 20 | 10 | 14 | 1 | 0 | 2 | 4 | 9 | 6 | 6 | 13 | 33 | 15 | 22 | 14 |
Golgi | 2 | 0 | 1 | 3 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 1 | 2 | 9 | 6 | 14 | 10 |
Mitochondria | 2 | 33 | 13 | 22 | 30 | 109 | 166 | 266 | 168 | 243 | 252 | 217 | 0 | 1 | 0 | 8 | 4 | 5 | 28 | 35 | 51 | 14 | 28 | 28 |
Nucleus | 1 | 1 | 0 | 0 | 0 | 3 | 4 | 13 | 2 | 4 | 2 | 1 | 1 | 1 | 0 | 61 | 26 | 30 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 3 | 3 | 15 | 17 | 3 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 2 | 3 | 1 | 0 | 9 | 2 | 1 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 5 | 5 | 17 | 17 | 8 | 11 | 6 | 18 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 2 | 2 | 0 | 1 |
Vac/Vac Membrane | 61 | 51 | 49 | 30 | 121 | 86 | 90 | 107 | 49 | 73 | 50 | 42 | 5 | 13 | 17 | 192 | 124 | 199 | 140 | 216 | 119 | 59 | 155 | 148 |
Unique Cell Count | 236 | 322 | 237 | 268 | 426 | 457 | 632 | 849 | 459 | 637 | 540 | 575 | 32 | 116 | 181 | 304 | 183 | 269 | 259 | 381 | 290 | 132 | 274 | 275 |
Labelled Cell Count | 261 | 354 | 266 | 298 | 496 | 529 | 764 | 1010 | 544 | 785 | 698 | 712 | 35 | 118 | 190 | 415 | 240 | 343 | 259 | 381 | 290 | 132 | 274 | 275 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 4.8 | 5.9 | 6.4 | 6.0 | 5.4 | 4.8 | 4.6 | 4.3 | 3.8 | 3.9 | 3.7 | 7.3 | 6.3 | 6.3 | 7.2 | 8.7 | 10.4 | 9.4 | 9.2 | 9.0 |
Std Deviation (1e-4) | 1.6 | 1.3 | 2.3 | 2.9 | 2.3 | 2.4 | 1.9 | 1.7 | 1.6 | 1.3 | 1.3 | 1.1 | 1.6 | 1.3 | 1.3 | 2.2 | 3.0 | 3.1 | 2.2 | 2.2 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.11 | 0.01 | -0.13 | -0.31 | -0.38 | -0.48 | -0.65 | -0.6 | -0.68 | 0.3 | 0.08 | 0.09 | 0.28 | 0.55 | 0.81 | 0.66 | 0.63 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 9.9 | 9.7 | 12.0 |