Standard name
Description Alpha 1,3 glucosyltransferase; involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; C998T transition in human ortholog ALG6 causes carbohydrate-deficient glycoprotein syndrome type-Ic; wild-type human ortholog ALG6 can partially complement yeast alg6 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0.11 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.08
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0.05 0.07 0.07 0.08 0.09 0.14 0 0 0 0 0 0
Cytoplasm 0.43 0.39 0.4 0.4 0.39 0.31 0.27 0.35 0.28 0.24 0.22 0.29 0.44 0.46 0.5 0.41 0.28 0.29 0.17 0.12 0.14 0.2 0.19 0.2
Endoplasmic Reticulum 0.1 0.42 0.45 0.33 0.21 0.14 0.27 0.2 0.22 0.32 0.22 0.18 0.51 0.44 0.41 0.06 0 0 0.58 0.57 0.49 0.56 0.51 0.49
Endosome 0.06 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Mitochondria 0.19 0 0.13 0.23 0.28 0.58 0.54 0.46 0.62 0.59 0.68 0.61 0 0 0 0.12 0.09 0.13 0.09 0.09 0.15 0.09 0.11 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.05 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.33 0.21 0.07 0.06 0.15 0.19 0.1 0.12 0.06 0.07 0.07 0.09 0 0.05 0.06 0.23 0.37 0.46 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 13 1 2 3 4 10 7 10 5 5 11 12 6 16 17 12 5 7 14 7 8 9
Bud 2 0 3 4 1 5 9 5 6 12 0 11 0 1 0 4 0 2 3 12 10 4 4 16
Bud Neck 0 0 0 0 0 0 1 1 0 3 0 0 0 0 0 0 0 0 2 2 2 0 1 3
Bud Site 0 0 0 0 0 0 0 1 1 2 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 1 7 8 5 2 6 12 13 6 11 13 25 25 18 16 15 2 0 2 0 0 0
Cytoplasm 103 98 115 81 69 72 95 115 87 98 35 59 120 152 173 87 48 32 50 35 47 44 39 41
Endoplasmic Reticulum 24 107 128 66 36 33 96 66 68 131 34 37 140 147 143 12 3 4 172 166 166 125 105 103
Endosome 15 8 0 3 9 6 4 5 0 0 0 0 2 2 1 22 6 3 4 6 3 5 4 1
Golgi 0 3 1 2 2 0 1 1 0 0 0 0 1 3 2 2 13 1 5 2 5 4 0 1
Mitochondria 46 7 36 47 49 138 188 149 191 243 108 124 9 6 4 26 15 14 27 25 51 20 23 9
Nucleus 1 0 1 0 0 2 2 4 0 1 1 1 0 0 1 1 2 0 0 0 0 0 0 0
Nuclear Periphery 6 7 14 5 5 4 16 7 9 21 4 8 9 12 8 1 0 0 6 12 9 3 1 2
Nucleolus 0 0 0 0 1 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5 3 3 0 0 1 0 1 0
SpindlePole 0 0 1 0 0 0 4 1 2 0 1 1 0 1 1 4 3 2 3 3 5 1 2 2
Vac/Vac Membrane 79 54 21 13 27 44 36 40 20 31 11 18 8 18 21 49 64 50 6 12 9 5 8 12
Unique Cell Count 237 253 286 201 175 236 350 325 308 415 158 203 275 334 349 214 171 109 296 293 337 224 209 211
Labelled Cell Count 279 288 334 229 209 312 458 412 403 565 205 275 313 379 387 247 190 138 296 293 337 224 209 211


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 7.2 5.8 5.5 6.2 5.2 4.8 5.0 4.9 5.0 4.8 5.0 6.9 6.7 6.6 7.2 8.7 8.7 6.4 6.6 7.5
Std Deviation (1e-4) 2.4 2.5 2.0 2.0 2.2 1.9 1.7 1.9 1.9 1.7 1.7 1.8 2.4 2.4 2.3 1.3 1.5 1.3 2.1 2.4 2.5
Intensity Change (Log2) – – – -0.08 0.09 -0.16 -0.28 -0.22 -0.25 -0.22 -0.28 -0.21 0.25 0.2 0.18 0.3 0.57 0.58 0.13 0.18 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.6 -2.0 -2.2 0 -1.0 -1.5 -1.6 0 -1.2 -0.3 -0.6 -2.1 1.4 2.3 2.4
Bud 0 0 0 0 0 0 1.7 0 2.9 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 3.0 2.6 0 0 3.3 4.4 4.3 4.7 4.9 0
Cytoplasm 0 -0.2 -2.3 -3.5 -1.2 -3.1 -4.7 -3.9 -2.5 0.8 1.3 2.4 0.1 -2.6 -2.0
Endoplasmic Reticulum -2.6 -5.3 -7.6 0 -6.5 -5.9 -3.6 0 -6.1 1.5 -0.2 -1.0 -9.6 -9.8 -7.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 5.5 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 4.4 0
Mitochondria 3.1 4.1 11.1 0 8.9 12.4 12.2 0 11.3 -4.1 -5.3 -5.9 -0.1 -1.3 0.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.4 -1.1 -2.0 0 -1.9 -1.2 0.1 0 -0.5 -1.0 -0.8 -1.8 -2.9 -2.9 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.4 2.8 3.9 1.3 2.0 -0.4 0.1 -0.1 0.6 -2.4 -1.0 -0.7 5.0 8.0 8.9