Standard name
Human Ortholog
Description Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; similar to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.08 0.08 0.08 0.1 0 0.07 0.13 0.07 0.14 0.06 0.06 0 0.09 0.08 0.07 0 0 0 0 0 0
Cytoplasm 0.98 1.0 0.96 0.87 0.88 0.88 0.76 0.8 0.73 0.75 0.76 0.7 0.97 0.99 1.0 0.9 0.92 0.95 0.95 0.94 0.9 0.88 0.87 0.76
Endoplasmic Reticulum 0 0 0 0.07 0.07 0.07 0.13 0.14 0.12 0.15 0.07 0.15 0 0 0 0 0 0 0 0 0 0 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Mitochondria 0 0 0 0 0 0 0 0 0.06 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 1 2 13 8 12 13 7 19 1 0 0 4 3 4 1 0 0 0 1 3
Bud 0 0 0 0 0 0 3 3 2 3 7 1 0 0 0 1 0 4 0 0 3 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 6 0 2 16 25 21 34 13 19 44 15 38 8 9 6 46 32 32 0 0 0 1 0 2
Cytoplasm 193 17 85 173 264 228 263 256 202 254 160 192 138 148 168 444 356 428 183 53 98 67 86 105
Endoplasmic Reticulum 1 0 3 14 20 18 46 44 32 50 15 40 3 4 0 13 9 14 4 0 3 3 2 6
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 1 2
Golgi 2 0 1 1 2 1 3 1 2 0 0 3 0 0 0 19 16 9 0 0 0 0 2 8
Mitochondria 0 0 0 0 2 1 10 4 16 13 22 10 0 0 0 3 6 4 0 0 0 0 0 0
Nucleus 0 0 0 1 1 1 0 3 0 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 2 5 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 1 10 7 3 15 7 13 15 5 14 0 0 0 13 4 4 0 0 0 0 2 1
Unique Cell Count 196 17 89 199 300 258 345 320 278 338 211 273 143 150 168 492 387 451 192 57 109 77 100 139
Labelled Cell Count 203 17 93 215 322 275 388 339 298 394 231 319 150 161 174 549 431 500 192 57 109 77 100 139


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.7 23.9 19.9 21.4 21.7 20.4 19.2 18.1 17.1 17.2 15.5 16.5 27.6 26.1 26.1 27.7 31.4 30.4 21.3 22.4 23.8
Std Deviation (1e-4) 3.7 3.0 3.6 4.3 4.8 4.0 4.0 4.1 4.1 4.2 5.0 4.7 6.1 4.8 5.0 6.9 7.7 6.6 6.3 4.6 5.8
Intensity Change (Log2) 0.1 0.13 0.03 -0.06 -0.14 -0.23 -0.21 -0.36 -0.27 0.47 0.39 0.39 0.48 0.66 0.61 0.09 0.17 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.9 2.0 1.9 2.3 0 1.6 2.9 1.7 3.1 0 0 0 2.2 2.0 1.7
Cytoplasm -2.2 -2.0 -2.0 -4.1 -3.5 -4.5 -4.2 -4.0 -4.9 0.4 1.5 2.8 -1.6 -1.1 -0.2
Endoplasmic Reticulum 1.2 1.2 1.2 2.6 2.7 2.3 2.9 1.2 2.9 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 3.2 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.1088 24.6371 21.5454 20.2858 17.6715 21.8235 20.225 26.3117 24.1622 22.0193 21.4336 22.8399 19.0616 24.5507 22.4691 21.1236 14.9478 22.3352
Actin 0.0092 0.0002 0.0052 0.0219 0.0028 0.0015 0.0017 0.0002 0.0003 0 0.0004 0.0016 0.0062 0.0034 0.0125 0.0008 0.0019 0.0005
Bud 0.0007 0.0001 0.0004 0.0002 0.0012 0.0003 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0003 0.0001 0.0004 0.0002 0.0069 0.0003
Bud Neck 0.0006 0.0002 0.0007 0.0006 0.0005 0.0009 0.0002 0.0002 0.0002 0.0003 0.0006 0.0008 0.0004 0.0001 0.0005 0.0002 0.0009 0.0006
Bud Periphery 0.001 0 0.0004 0.0001 0.0031 0.0002 0.0003 0 0.0001 0 0.0007 0.0001 0.0003 0.0001 0.0003 0.0001 0.0077 0.0001
Bud Site 0.0015 0.0002 0.0021 0.001 0.0007 0.0002 0.0003 0.0001 0.0001 0 0.0001 0 0.0014 0.0001 0.0005 0.0001 0.0043 0.0001
Cell Periphery 0.0006 0.0001 0.0004 0.0001 0.0017 0.0001 0.0002 0.0001 0.0001 0 0.003 0.0001 0.0002 0.0001 0.0001 0.0001 0.001 0
Cytoplasm 0.8207 0.9795 0.9153 0.8856 0.7473 0.9321 0.9297 0.9722 0.9718 0.9892 0.9515 0.9479 0.9159 0.982 0.9464 0.93 0.6896 0.9492
Cytoplasmic Foci 0.0285 0.0033 0.0094 0.0106 0.0096 0.0103 0.0044 0.0011 0.0049 0.0011 0.0038 0.003 0.0088 0.0011 0.0041 0.0043 0.0338 0.0054
Eisosomes 0.0001 0 0.0002 0.0001 0.0001 0 0 0 0 0 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0254 0.0049 0.007 0.0253 0.015 0.0199 0.0336 0.0226 0.0108 0.0034 0.018 0.0341 0.019 0.0079 0.0103 0.0106 0.0072 0.03
Endosome 0.0493 0.006 0.0227 0.0234 0.0459 0.0134 0.0056 0.0008 0.0028 0.0014 0.0059 0.0047 0.0176 0.0015 0.0089 0.0105 0.0995 0.0063
Golgi 0.0117 0.0013 0.006 0.0105 0.0224 0.0046 0.0012 0.0001 0.0007 0 0.001 0.0016 0.0035 0.0015 0.0019 0.0089 0.0178 0.0006
Lipid Particles 0.008 0.0003 0.0037 0.0019 0.0059 0.0006 0.0013 0 0.0016 0 0.0005 0.0005 0.0035 0 0.0027 0.0012 0.0285 0.0004
Mitochondria 0.0139 0.0001 0.0171 0.0046 0.0915 0.0073 0.0024 0 0.001 0 0.0038 0.0007 0.0051 0.0005 0.0027 0.021 0.0321 0.0011
None 0.0019 0.0002 0.0015 0.0004 0.0009 0.0005 0.0027 0.0001 0.0002 0.0002 0.0005 0.0004 0.0017 0.0001 0.0005 0.0003 0.0012 0.0003
Nuclear Periphery 0.005 0.0006 0.0009 0.0022 0.0238 0.0013 0.005 0.0006 0.0007 0.0007 0.0011 0.0013 0.0043 0.0006 0.002 0.0043 0.002 0.0016
Nucleolus 0.0002 0.0001 0.0001 0 0.0004 0 0.0002 0 0 0 0.0001 0 0.0003 0 0.0001 0 0.0008 0
Nucleus 0.0017 0.0012 0.0009 0.0011 0.0075 0.0017 0.003 0.0007 0.0007 0.001 0.001 0.0013 0.0019 0.0004 0.001 0.0011 0.0025 0.0009
Peroxisomes 0.0068 0 0.0016 0.0047 0.0013 0.0014 0.0005 0 0.0006 0 0 0 0.0029 0 0.0007 0.0002 0.0199 0.0001
Punctate Nuclear 0.0029 0.0001 0.0002 0.0002 0.0006 0.0002 0.0027 0 0.0001 0 0.0001 0.0001 0.0018 0 0.0006 0.0001 0.0005 0.0001
Vacuole 0.007 0.0013 0.0035 0.0045 0.0097 0.0026 0.0041 0.0009 0.003 0.0021 0.006 0.0013 0.0037 0.0003 0.0027 0.0035 0.0293 0.0018
Vacuole Periphery 0.0032 0.0001 0.0007 0.001 0.0081 0.0009 0.0007 0.0001 0.0002 0.0003 0.0015 0.0003 0.0012 0.0001 0.0009 0.0026 0.0126 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 288.3055 330.6871 337.491 387.478 362.1194 346.4107 362.3802 464.9163 461.4006 352.8765
Translational Efficiency 2.1279 1.9362 1.7377 1.6795 1.5493 1.7593 1.8307 1.5728 1.6428 1.7209

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1229 841 2341 1372 1740 1711 229 1314 2969 2552 2570 2686

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1764.80 2216.17 2284.82 2700.29 1855.68 2240.16 2591.37 2814.45 1818.06 2232.25 2312.14 2756.14
Standard Deviation 337.02 424.09 440.74 809.60 319.53 418.43 397.36 708.41 329.93 420.45 445.69 763.91
Intensity Change Log 2 0.328564 0.372576 0.613610 0.271654 0.481767 0.600905 0.299675 0.429573 0.607112

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000550 0.001309 0.000635 0.001756 0.000352 0.000483 0.001120 0.001809 0.000434 0.000755 0.000678 0.001782
Bud Neck 0.000662 0.001413 0.001477 0.002275 0.000532 0.001038 0.009029 0.004334 0.000586 0.001162 0.002150 0.003282
Bud Site 0.013705 0.018621 0.015781 0.034252 0.009506 0.015004 0.030421 0.054381 0.011244 0.016196 0.017086 0.044099
Cell Periphery 0.004732 0.005057 0.007620 0.013644 0.001710 0.001447 0.019823 0.007986 0.002961 0.002636 0.008708 0.010876
Cytoplasm 0.842194* 0.813473* 0.864264* 0.799710* 0.890948* 0.879924* 0.582671* 0.703059* 0.870767* 0.858025* 0.839172* 0.752428*
Cytoplasmic Foci 0.040546 0.035853 0.011422 0.023541 0.028429 0.026829 0.025058 0.067393 0.033445 0.029803 0.012637 0.044994
Eisosomes 0.000006 0.000006 0.000006 0.000011 0.000003 0.000003 0.000014 0.000010 0.000005 0.000004 0.000006 0.000010
Endoplasmic Reticulum 0.010008 0.005178 0.000413 0.000365 0.005637 0.002310 0.003458 0.000307 0.007446 0.003255 0.000684 0.000337
Endosome 0.004169 0.005345 0.001040 0.001639 0.002757 0.003477 0.005577 0.004611 0.003341 0.004092 0.001444 0.003093
Golgi 0.026525 0.040110 0.006253 0.007498 0.021657 0.019174 0.029378 0.012994 0.023672 0.026073 0.008314 0.010187
Lipid Particles 0.017714 0.018774 0.016753 0.023661 0.007168 0.010271 0.117868* 0.028118 0.011533 0.013073 0.025763 0.025841
Mitochondria 0.000818 0.001220 0.000125 0.001086 0.000200 0.000349 0.000036 0.000922 0.000456 0.000636 0.000117 0.001006
Mitotic Spindle 0.002021 0.005458 0.000797 0.002524 0.002154 0.006658 0.003748 0.010203 0.002099 0.006263 0.001060 0.006281
None 0.000714 0.000773 0.000811 0.003484 0.000964 0.001133 0.000586 0.000634 0.000861 0.001014 0.000791 0.002090
Nuclear Periphery 0.000045 0.000101 0.000057 0.000105 0.000045 0.000091 0.000390 0.000146 0.000045 0.000094 0.000087 0.000125
Nuclear Periphery Foci 0.005161 0.009515 0.003201 0.003961 0.004361 0.004242 0.025364 0.004212 0.004692 0.005980 0.005176 0.004084
Nucleolus 0.000041 0.000092 0.000088 0.000473 0.000074 0.000043 0.000064 0.000116 0.000060 0.000059 0.000086 0.000299
Nucleus 0.002013 0.003421 0.004785 0.008208 0.002507 0.003769 0.012455 0.006984 0.002302 0.003655 0.005469 0.007609
Peroxisomes 0.000151 0.000564 0.000308 0.000730 0.000164 0.000208 0.000146 0.001322 0.000158 0.000325 0.000294 0.001020
Vacuole 0.018445 0.021558 0.060648 0.068071 0.012384 0.016870 0.128000 0.084529 0.014893 0.018415 0.066649 0.076122
Vacuole Periphery 0.009781 0.012158 0.003516 0.003005 0.008448 0.006677 0.004793 0.005928 0.009000 0.008483 0.003629 0.004435

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.31 -0.57 -2.50 -0.53 -2.15 -1.01 -4.80 -3.20 -2.76 -1.40 -1.59 -1.47 -4.02 -2.45 -2.92
Bud Neck -4.24 -4.37 -9.03 -4.50 -4.19 -3.57 -8.64 -13.57 -11.39 4.33 -5.23 -8.11 -16.19 -11.99 -5.69
Bud Site -2.40 -1.38 -7.95 -5.62 -6.82 -3.54 -5.86 -13.45 -11.38 -4.82 -3.99 -4.45 -15.57 -12.53 -11.53
Cell Periphery -0.32 -3.15 -5.97 -5.61 -4.15 1.39 -9.76 -8.65 -9.18 6.23 0.78 -9.09 -9.62 -10.27 -1.88
Cytoplasm 2.76 -2.41 6.03 2.52 9.04 1.89 16.29 20.72 19.06 -4.59 2.46 5.81 19.72 16.73 13.96
Cytoplasmic Foci 1.30 10.95 2.83 1.67 -5.17 0.44 0.38 -9.73 -9.81 -9.23 1.65 11.16 -6.59 -7.42 -12.55
Eisosomes 0.42 0.48 -2.97 -3.35 -3.39 0.41 -8.87 -4.13 -4.47 2.09 0.95 -1.84 -5.30 -6.11 -3.32
Endoplasmic Reticulum 3.19 7.61 7.65 5.66 0.55 3.53 1.24 6.48 4.28 1.86 5.09 9.28 9.99 6.91 2.14
Endosome -1.91 8.94 5.65 5.90 -1.74 -1.48 -3.47 -2.30 -1.12 1.23 -1.97 7.87 1.03 2.41 -3.98
Golgi -4.46 12.33 10.17 11.55 -0.73 1.35 -1.45 5.25 3.85 4.09 -1.43 13.22 10.70 10.76 -1.14
Lipid Particles -0.57 0.31 -2.90 -2.01 -3.42 -2.82 -10.77 -9.58 -7.69 8.40 -1.49 -8.41 -9.56 -7.91 -0.41
Mitochondria -0.34 1.26 -0.29 0.17 -1.88 -0.80 2.93 -2.42 -1.55 -3.16 -0.41 1.48 -1.30 -0.64 -2.95
Mitotic Spindle -2.38 1.63 -1.47 1.22 -2.59 -3.62 -2.23 -5.16 -2.53 -4.37 -4.35 1.75 -5.24 -1.16 -6.01
None -0.37 -0.73 -2.63 -2.58 -2.54 -0.52 1.82 1.56 1.77 -0.68 -0.71 0.48 -2.24 -1.89 -2.41
Nuclear Periphery -4.25 -1.47 -4.94 -1.47 -4.38 -1.38 -7.94 -6.52 -2.14 4.58 -2.13 -5.26 -8.19 -2.13 -4.59
Nuclear Periphery Foci -4.54 3.88 1.47 5.43 -2.35 -0.04 -10.10 -0.10 -0.06 10.12 -2.71 -1.13 0.70 3.45 1.90
Nucleolus -0.91 -3.10 -1.34 -1.17 -1.21 0.66 0.19 -0.90 -6.89 -4.45 0.09 -0.74 -1.44 -1.47 -1.32
Nucleus -4.74 -9.18 -11.16 -8.73 -6.36 -3.54 -7.80 -9.08 -7.79 2.69 -5.61 -9.82 -13.94 -11.55 -6.72
Peroxisomes -1.77 -3.53 -3.13 -0.29 -2.18 -0.48 0.22 -4.69 -4.78 -5.11 -1.78 -2.09 -5.50 -3.82 -4.73
Vacuole -1.20 -14.52 -13.90 -12.37 -2.74 -2.69 -12.20 -18.20 -17.06 3.93 -2.51 -20.47 -23.09 -21.53 -3.96
Vacuole Periphery -1.13 5.36 5.55 5.06 1.00 1.62 3.62 2.81 1.23 -0.61 0.54 7.54 6.07 4.73 -0.72