Standard name
Description Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; similar to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.08 0.08 0.08 0.1 0 0.07 0.13 0.07 0.14 0.06 0.06 0 0.09 0.08 0.07 0 0 0 0 0 0
Cytoplasm 0.98 1.0 0.96 0.87 0.88 0.88 0.76 0.8 0.73 0.75 0.76 0.7 0.97 0.99 1.0 0.9 0.92 0.95 0.95 0.94 0.9 0.88 0.87 0.76
Endoplasmic Reticulum 0 0 0 0.07 0.07 0.07 0.13 0.14 0.12 0.15 0.07 0.15 0 0 0 0 0 0 0 0 0 0 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Mitochondria 0 0 0 0 0 0 0 0 0.06 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 1 2 13 8 12 13 7 19 1 0 0 4 3 4 1 0 0 0 1 3
Bud 0 0 0 0 0 0 3 3 2 3 7 1 0 0 0 1 0 4 0 0 3 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 0 2 16 25 21 34 13 19 44 15 38 8 9 6 46 32 32 0 0 0 1 0 2
Cytoplasm 193 17 85 173 264 228 263 256 202 254 160 192 138 148 168 444 356 428 183 53 98 67 86 105
Endoplasmic Reticulum 1 0 3 14 20 18 46 44 32 50 15 40 3 4 0 13 9 14 4 0 3 3 2 6
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 1 2
Golgi 2 0 1 1 2 1 3 1 2 0 0 3 0 0 0 19 16 9 0 0 0 0 2 8
Mitochondria 0 0 0 0 2 1 10 4 16 13 22 10 0 0 0 3 6 4 0 0 0 0 0 0
Nucleus 0 0 0 1 1 1 0 3 0 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 2 5 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 1 10 7 3 15 7 13 15 5 14 0 0 0 13 4 4 0 0 0 0 2 1
Unique Cell Count 196 17 89 199 300 258 345 320 278 338 211 273 143 150 168 492 387 451 192 57 109 77 100 139
Labelled Cell Count 203 17 93 215 322 275 388 339 298 394 231 319 150 161 174 549 431 500 192 57 109 77 100 139


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.7 23.9 19.9 21.4 21.7 20.4 19.2 18.1 17.1 17.2 15.5 16.5 27.6 26.1 26.1 27.7 31.4 30.4 21.3 22.4 23.8
Std Deviation (1e-4) 3.7 3.0 3.6 4.3 4.8 4.0 4.0 4.1 4.1 4.2 5.0 4.7 6.1 4.8 5.0 6.9 7.7 6.6 6.3 4.6 5.8
Intensity Change (Log2) – – – 0.1 0.13 0.03 -0.06 -0.14 -0.23 -0.21 -0.36 -0.27 0.47 0.39 0.39 0.48 0.66 0.61 0.09 0.17 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.9 2.0 1.9 2.3 0 1.6 2.9 1.7 3.1 0 0 0 2.2 2.0 1.7
Cytoplasm -2.2 -2.0 -2.0 -4.1 -3.5 -4.5 -4.2 -4.0 -4.9 0.4 1.5 2.8 -1.6 -1.1 -0.2
Endoplasmic Reticulum 1.2 1.2 1.2 2.6 2.7 2.3 2.9 1.2 2.9 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 3.2 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0