Standard name | |
---|---|
Human Ortholog | |
Description | Kinetochore checkpoint WD40 repeat protein; localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p; functions at kinetochore to activate APC/C-Cdc20p for normal mitotic progression |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 4 | 2 | 8 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 1 | 4 | 13 | 9 |
Bud Neck | 0 | 0 | 3 | 2 | 8 | 1 | 4 | 8 | 2 | 1 | 0 | 3 | 5 | 8 | 5 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 9 | 13 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 8 | 4 | 6 | 7 | 6 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 41 | 32 | 42 | 46 | 68 | 84 | 95 | 118 | 64 | 79 | 70 | 73 | 116 | 180 | 234 | 27 | 34 | 24 | 38 | 17 | 24 | 59 | 118 | 123 |
Endoplasmic Reticulum | 2 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 2 | 10 | 5 | 9 | 0 | 0 | 0 | 0 | 2 | 3 |
Endosome | 3 | 7 | 3 | 6 | 7 | 4 | 0 | 2 | 2 | 2 | 0 | 1 | 7 | 32 | 32 | 12 | 16 | 21 | 3 | 4 | 1 | 1 | 5 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 |
Mitochondria | 71 | 4 | 3 | 10 | 19 | 92 | 156 | 155 | 144 | 124 | 227 | 130 | 9 | 0 | 3 | 12 | 5 | 8 | 9 | 5 | 9 | 11 | 27 | 20 |
Nucleus | 51 | 73 | 33 | 55 | 71 | 67 | 58 | 109 | 50 | 49 | 87 | 65 | 110 | 107 | 117 | 50 | 97 | 103 | 83 | 98 | 49 | 88 | 136 | 110 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Nucleolus | 4 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 2 | 3 | 2 | 1 | 4 | 2 | 2 | 11 | 12 | 22 | 0 | 1 | 1 | 0 | 1 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 7 | 3 | 10 | 25 | 30 | 15 | 17 | 20 | 5 | 12 | 2 | 11 | 14 | 47 | 30 | 21 | 11 | 17 | 20 | 14 | 21 | 9 | 20 | 31 |
Vac/Vac Membrane | 35 | 63 | 6 | 10 | 33 | 23 | 20 | 20 | 13 | 20 | 14 | 16 | 15 | 47 | 38 | 26 | 30 | 39 | 45 | 39 | 39 | 82 | 142 | 151 |
Unique Cell Count | 164 | 159 | 98 | 134 | 210 | 236 | 287 | 350 | 223 | 231 | 312 | 236 | 263 | 392 | 433 | 130 | 176 | 192 | 211 | 193 | 158 | 270 | 498 | 493 |
Labelled Cell Count | 214 | 183 | 102 | 156 | 239 | 290 | 362 | 445 | 292 | 299 | 417 | 314 | 285 | 428 | 467 | 170 | 210 | 245 | 211 | 193 | 158 | 270 | 498 | 493 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 5.0 | 5.2 | 5.1 | 4.8 | 3.9 | 3.6 | 4.1 | 3.8 | 3.8 | 3.2 | 3.7 | 4.8 | 5.4 | 5.3 | 5.8 | 6.6 | 6.4 | 4.9 | 5.3 | 5.5 |
Std Deviation (1e-4) | 0.9 | 1.3 | 1.6 | 2.5 | 1.3 | 1.6 | 1.3 | 1.5 | 2.8 | 1.8 | 0.7 | 0.8 | 1.1 | 1.4 | 1.3 | 1.5 | 1.8 | 1.5 | 1.7 | 1.8 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.11 | -0.43 | -0.52 | -0.33 | -0.45 | -0.44 | -0.71 | -0.49 | -0.11 | 0.05 | 0.04 | 0.18 | 0.36 | 0.3 | -0.09 | 0.04 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.3 | -1.8 | -1.2 | -1.7 | -1.7 | -2.5 | -1.5 | -4.0 | -2.1 | 0.2 | 0.5 | 2.0 | -3.6 | -4.2 | -5.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 1.6 | 1.9 | 1.9 | 2.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 0 | 0 |
Nucleus | 1.1 | 0 | -1.0 | -2.7 | -0.5 | -2.1 | -2.4 | -1.1 | -1.1 | 1.4 | -1.2 | -1.3 | 0.7 | 3.4 | 3.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 3.1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | -1.1 | 1.3 | -1.2 | -0.4 |
Vacuole | 0.4 | 2.4 | 1.1 | 0.3 | -0.2 | -0.1 | 0.8 | 0 | 0.2 | -0.2 | 1.7 | 0.9 | 3.0 | 2.6 | 3.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.7603 | -0.1122 | -0.6005 | 0.0914 | -1.1146 | -0.4811 | 0.5561 | 0.2925 | 0.4045 | -0.2154 | 0.2849 | 0.2239 | -0.4436 | -0.1832 | -0.1464 | -0.2255 | -0.1178 | -0.2317 |
Actin | 0.0045 | 0.0002 | 0.0005 | 0.0006 | 0.0001 | 0.0002 | 0.0279 | 0.001 | 0.0142 | 0.0006 | 0.0089 | 0.0018 | 0.0105 | 0.0001 | 0.0001 | 0.0001 | 0.0018 | 0.0031 |
Bud | 0.0003 | 0.0018 | 0 | 0.0001 | 0 | 0.0018 | 0.0008 | 0.0003 | 0.0014 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.0017 | 0.0006 | 0.0009 | 0.0006 | 0.0006 | 0.0022 | 0.0052 | 0.0011 | 0.0015 | 0.0004 | 0.0006 | 0.0027 | 0.0019 | 0.0006 | 0.0007 | 0.0003 | 0.0005 | 0.0028 |
Bud Periphery | 0.0003 | 0.0003 | 0 | 0 | 0 | 0.0005 | 0.0015 | 0.0003 | 0.0014 | 0 | 0.0005 | 0.0006 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0019 | 0.0017 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0051 | 0.0023 | 0.0093 | 0 | 0.0001 | 0.0003 | 0.0006 | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0578 | 0.0705 | 0.0253 | 0.0205 | 0.0329 | 0.0414 | 0.0587 | 0.0772 | 0.0353 | 0.0071 | 0.0465 | 0.0287 | 0.0744 | 0.0687 | 0.0262 | 0.0294 | 0.0251 | 0.0281 |
Cytoplasmic Foci | 0.0085 | 0.0026 | 0.0042 | 0.0009 | 0.0008 | 0.0046 | 0.0225 | 0.0091 | 0.0098 | 0.0001 | 0.0038 | 0.0053 | 0.007 | 0.0065 | 0.0035 | 0.0007 | 0.0025 | 0.0013 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0021 | 0.0019 | 0.0005 | 0.0015 | 0.0003 | 0.0007 | 0.0056 | 0.0017 | 0.0026 | 0.0005 | 0.0014 | 0.0011 | 0.0028 | 0.0018 | 0.0006 | 0.0005 | 0.0008 | 0.0009 |
Endosome | 0.0073 | 0.0031 | 0.0021 | 0.0025 | 0.0007 | 0.0025 | 0.0371 | 0.0047 | 0.0158 | 0.0007 | 0.0048 | 0.0051 | 0.0082 | 0.0025 | 0.0011 | 0.0008 | 0.0055 | 0.0022 |
Golgi | 0.0012 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0107 | 0.0003 | 0.0047 | 0 | 0.0087 | 0.0008 | 0.0014 | 0.0001 | 0.0001 | 0 | 0.0006 | 0.0004 |
Lipid Particles | 0.005 | 0.0003 | 0.001 | 0.0001 | 0.0001 | 0.0003 | 0.0259 | 0.001 | 0.0036 | 0 | 0.0031 | 0.0036 | 0.0047 | 0.0004 | 0.0004 | 0 | 0.0035 | 0.0007 |
Mitochondria | 0.0012 | 0.0007 | 0.0048 | 0.0009 | 0.0003 | 0.0004 | 0.0127 | 0.0014 | 0.0071 | 0.0015 | 0.0702 | 0.0041 | 0.0011 | 0.0004 | 0.0002 | 0.0004 | 0.0011 | 0.0006 |
None | 0.15 | 0.1491 | 0.0997 | 0.1402 | 0.0844 | 0.0866 | 0.1216 | 0.1444 | 0.0528 | 0.0112 | 0.0784 | 0.0728 | 0.1137 | 0.1019 | 0.071 | 0.0128 | 0.0489 | 0.051 |
Nuclear Periphery | 0.0233 | 0.0248 | 0.0198 | 0.0247 | 0.0176 | 0.012 | 0.0156 | 0.011 | 0.0123 | 0.0298 | 0.0157 | 0.0229 | 0.0188 | 0.013 | 0.0082 | 0.0095 | 0.0153 | 0.0184 |
Nucleolus | 0.0131 | 0.0156 | 0.0145 | 0.0057 | 0.0088 | 0.0146 | 0.0173 | 0.0189 | 0.0227 | 0.006 | 0.006 | 0.0188 | 0.0127 | 0.0136 | 0.0112 | 0.0046 | 0.0102 | 0.0126 |
Nucleus | 0.5487 | 0.6069 | 0.5797 | 0.7275 | 0.7544 | 0.6791 | 0.5367 | 0.5726 | 0.5848 | 0.9125 | 0.6288 | 0.7287 | 0.6192 | 0.6517 | 0.7008 | 0.8913 | 0.7791 | 0.7505 |
Peroxisomes | 0.0028 | 0.0001 | 0.0013 | 0.0001 | 0 | 0.0001 | 0.0168 | 0.0003 | 0.0022 | 0.0001 | 0.0006 | 0.0046 | 0.0053 | 0.0001 | 0.0001 | 0 | 0.0006 | 0.0001 |
Punctate Nuclear | 0.168 | 0.1168 | 0.2445 | 0.0726 | 0.0985 | 0.1519 | 0.0706 | 0.15 | 0.2155 | 0.0288 | 0.1193 | 0.0962 | 0.1144 | 0.1365 | 0.1747 | 0.0489 | 0.1028 | 0.1263 |
Vacuole | 0.0013 | 0.0024 | 0.0004 | 0.0007 | 0.0003 | 0.0007 | 0.0051 | 0.0019 | 0.0017 | 0.0002 | 0.0007 | 0.0009 | 0.0022 | 0.001 | 0.0005 | 0.0003 | 0.0006 | 0.0005 |
Vacuole Periphery | 0.0005 | 0.0005 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0016 | 0.0004 | 0.001 | 0.0004 | 0.0013 | 0.001 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.5843 | 13.4593 | 17.8346 | 17.9711 | 20.9154 | 22.5718 | 19.441 | 23.6185 | 16.4382 | 24.2998 |
Translational Efficiency | 0.6742 | 0.4819 | 0.1179 | 0.2659 | 0.5585 | 0.6359 | 0.2328 | 0.1695 | 0.2878 | 0.4849 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1554 | 588 | 2120 | 1012 | 1806 | 1265 | 600 | 1119 | 3360 | 1853 | 2720 | 2131 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 664.42 | 758.23 | 771.54 | 774.48 | 660.55 | 731.02 | 832.55 | 833.59 | 662.34 | 739.65 | 785.00 | 805.52 |
Standard Deviation | 78.01 | 105.67 | 98.89 | 106.91 | 70.48 | 88.35 | 104.83 | 120.32 | 74.08 | 95.04 | 103.38 | 117.90 |
Intensity Change Log 2 | — | 0.190540 | 0.215645 | 0.221132 | — | 0.146243 | 0.333869 | 0.335670 | — | 0.168626 | 0.275795 | 0.279371 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000103 | 0.001370 | 0.000255 | 0.001878 | 0.000071 | 0.001072 | 0.000430 | 0.003296 | 0.000086 | 0.001167 | 0.000294 | 0.002622 |
Bud Neck | 0.004264 | 0.022982 | 0.010083 | 0.019932 | 0.004413 | 0.015108 | 0.001680 | 0.011069 | 0.004344 | 0.017607 | 0.008229 | 0.015278 |
Bud Site | 0.001543 | 0.016813 | 0.003361 | 0.082982 | 0.000785 | 0.011205 | 0.000867 | 0.040670 | 0.001136 | 0.012985 | 0.002811 | 0.060764 |
Cell Periphery | 0.000151 | 0.001152 | 0.000079 | 0.000378 | 0.000118 | 0.000333 | 0.000217 | 0.000231 | 0.000133 | 0.000593 | 0.000110 | 0.000301 |
Cytoplasm | 0.224318* | 0.188366* | 0.038496 | 0.200216* | 0.211668* | 0.208216* | 0.060528 | 0.227107* | 0.217519* | 0.201917* | 0.043356 | 0.214337* |
Cytoplasmic Foci | 0.083911 | 0.082316 | 0.000295 | 0.010379 | 0.046140 | 0.051208 | 0.007438 | 0.012536 | 0.063609 | 0.061079 | 0.001871 | 0.011511 |
Eisosomes | 0.000075 | 0.000639 | 0.000012 | 0.000057 | 0.000046 | 0.000064 | 0.000057 | 0.000049 | 0.000060 | 0.000246 | 0.000022 | 0.000053 |
Endoplasmic Reticulum | 0.001159 | 0.006588 | 0.002920 | 0.007755 | 0.000900 | 0.001879 | 0.003188 | 0.005739 | 0.001020 | 0.003374 | 0.002979 | 0.006697 |
Endosome | 0.002974 | 0.012824 | 0.000285 | 0.006344 | 0.001410 | 0.004507 | 0.001255 | 0.013757 | 0.002133 | 0.007146 | 0.000499 | 0.010236 |
Golgi | 0.001171 | 0.003361 | 0.000035 | 0.006919 | 0.000345 | 0.001869 | 0.000910 | 0.006930 | 0.000727 | 0.002342 | 0.000228 | 0.006925 |
Lipid Particles | 0.010607 | 0.009277 | 0.000168 | 0.001367 | 0.005147 | 0.005790 | 0.001059 | 0.001631 | 0.007672 | 0.006897 | 0.000365 | 0.001506 |
Mitochondria | 0.006770 | 0.014566 | 0.000844 | 0.012480 | 0.003509 | 0.007156 | 0.000691 | 0.004968 | 0.005017 | 0.009507 | 0.000810 | 0.008535 |
Mitotic Spindle | 0.001205 | 0.029364 | 0.001382 | 0.046352 | 0.001659 | 0.013724 | 0.001138 | 0.065997 | 0.001449 | 0.018687 | 0.001328 | 0.056668 |
None | 0.012392 | 0.012299 | 0.004624 | 0.008841 | 0.007115 | 0.011160 | 0.011897 | 0.010918 | 0.009555 | 0.011522 | 0.006229 | 0.009931 |
Nuclear Periphery | 0.002126 | 0.002172 | 0.001238 | 0.003041 | 0.001471 | 0.001651 | 0.000802 | 0.003055 | 0.001774 | 0.001817 | 0.001142 | 0.003049 |
Nuclear Periphery Foci | 0.001066 | 0.002873 | 0.000227 | 0.002750 | 0.000723 | 0.000975 | 0.000306 | 0.002070 | 0.000881 | 0.001578 | 0.000244 | 0.002393 |
Nucleolus | 0.009326 | 0.011298 | 0.001372 | 0.002347 | 0.006820 | 0.012500 | 0.001754 | 0.001558 | 0.007979 | 0.012119 | 0.001456 | 0.001932 |
Nucleus | 0.623235* | 0.523191* | 0.930530* | 0.526735* | 0.699511* | 0.630352* | 0.899535* | 0.533391* | 0.664233* | 0.596348* | 0.923693* | 0.530230* |
Peroxisomes | 0.006578 | 0.020677 | 0.000048 | 0.005551 | 0.002388 | 0.007640 | 0.000414 | 0.003128 | 0.004326 | 0.011777 | 0.000129 | 0.004279 |
Vacuole | 0.006601 | 0.035099 | 0.003650 | 0.047990 | 0.005553 | 0.012707 | 0.005621 | 0.048985 | 0.006038 | 0.019812 | 0.004085 | 0.048512 |
Vacuole Periphery | 0.000424 | 0.002772 | 0.000094 | 0.005708 | 0.000211 | 0.000884 | 0.000212 | 0.002915 | 0.000309 | 0.001483 | 0.000120 | 0.004241 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -7.98 | -10.95 | -9.89 | -0.01 | -7.53 | -1.35 | -4.51 | -4.90 | -0.98 | -2.25 | -2.40 | -10.08 | -8.38 | -0.99 | -5.81 |
Bud Neck | -7.65 | -8.84 | -10.13 | 0.60 | -3.10 | -6.32 | 0.70 | -7.87 | 0.30 | -8.36 | -9.75 | -8.29 | -12.77 | -0.09 | -3.76 |
Bud Site | -6.29 | -3.06 | -9.62 | -5.48 | -8.98 | -5.41 | -3.31 | -8.42 | -3.48 | -7.64 | -7.79 | -4.49 | -12.52 | -6.85 | -11.23 |
Cell Periphery | -1.13 | 6.54 | -4.63 | 0.95 | -7.07 | -2.04 | -2.91 | -3.53 | 1.47 | 0.88 | -1.60 | 2.61 | -5.78 | 1.23 | -6.89 |
Cytoplasm | 3.36 | 30.40 | 6.91 | 2.29 | -18.16 | 0.50 | 22.63 | 5.38 | 4.39 | -14.24 | 2.38 | 41.43 | 8.65 | 5.21 | -25.06 |
Cytoplasmic Foci | 1.25 | 23.42 | 20.53 | 12.53 | -7.04 | -0.37 | 15.27 | 15.62 | 14.53 | -1.36 | 1.93 | 29.14 | 25.53 | 19.06 | -8.98 |
Eisosomes | -1.10 | 16.97 | -0.43 | 1.10 | -14.09 | -3.32 | -2.76 | -5.01 | -1.10 | -0.34 | -1.15 | 12.22 | -3.76 | 1.06 | -14.40 |
Endoplasmic Reticulum | -10.24 | -14.05 | -16.28 | -5.73 | -11.31 | -4.57 | -11.16 | -17.42 | -13.99 | -3.04 | -10.31 | -19.78 | -23.34 | -14.83 | -12.60 |
Endosome | -5.62 | 5.91 | -2.07 | 4.73 | -8.22 | -5.35 | 0.69 | -4.66 | -1.34 | -4.54 | -7.16 | 6.75 | -5.08 | 2.13 | -8.54 |
Golgi | -2.55 | 5.32 | -1.34 | 0.55 | -2.58 | -2.20 | -0.43 | -2.15 | -0.32 | -1.57 | -2.90 | 3.69 | -2.46 | -0.09 | -3.39 |
Lipid Particles | 1.16 | 13.25 | 10.87 | 8.94 | -13.58 | -1.21 | 8.42 | 7.59 | 8.09 | -2.66 | 1.00 | 16.57 | 13.18 | 11.66 | -14.92 |
Mitochondria | -2.58 | 4.27 | 0.48 | 3.01 | -4.17 | -3.12 | 3.27 | 0.79 | 4.53 | -4.65 | -3.50 | 5.33 | 0.77 | 4.47 | -5.56 |
Mitotic Spindle | -6.04 | -2.36 | -10.49 | -3.52 | -10.04 | -5.35 | -1.68 | -11.01 | -7.58 | -9.91 | -8.03 | -2.93 | -15.20 | -8.59 | -14.42 |
None | 0.86 | 6.53 | 3.76 | 1.60 | -4.40 | -2.90 | -1.18 | -1.27 | 1.91 | 0.53 | -1.04 | 5.34 | 2.22 | 2.62 | -3.64 |
Nuclear Periphery | -0.26 | 0.64 | -5.07 | -6.15 | -7.27 | -1.10 | -0.33 | -13.20 | -11.49 | -13.30 | -0.39 | 0.20 | -9.82 | -11.24 | -12.36 |
Nuclear Periphery Foci | -2.75 | 0.69 | -6.01 | -4.21 | -6.22 | -0.26 | -0.32 | -3.90 | -3.71 | -3.23 | -1.97 | 0.22 | -7.10 | -6.03 | -6.97 |
Nucleolus | 0.12 | 4.54 | 5.00 | 3.66 | 0.61 | -2.02 | 4.18 | 4.83 | 6.01 | 0.28 | -1.60 | 5.88 | 6.93 | 7.01 | 1.06 |
Nucleus | 6.11 | -35.21 | 4.67 | -2.08 | 33.03 | 5.92 | -19.08 | 9.57 | 3.57 | 24.62 | 7.09 | -42.07 | 10.17 | 2.34 | 43.87 |
Peroxisomes | -5.14 | 9.60 | 4.64 | 6.97 | -2.95 | -5.25 | 6.42 | 3.81 | 7.03 | -2.50 | -6.62 | 12.15 | 5.76 | 9.44 | -4.18 |
Vacuole | -9.11 | 0.50 | -17.04 | -9.02 | -17.03 | -6.28 | -3.99 | -18.93 | -17.53 | -15.26 | -10.85 | -2.76 | -25.42 | -20.50 | -24.37 |
Vacuole Periphery | -4.12 | 4.50 | -2.73 | 0.34 | -3.20 | -4.18 | 0.12 | -2.86 | -0.63 | -2.77 | -5.54 | 4.15 | -3.73 | -0.59 | -4.23 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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