Standard name
Human Ortholog
Description Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.85 0.8 0.98 0.97 0.98 0.96 0.98 0.98 0.93 0.89 0.92 0.84 0.94 0.96 0.91 0.93 0.87 0.4 0.36 0.52 0.27 0.28 0.28
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.11 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.14 0 0 0 0 0 0 0.13 0.18 0.09 0 0 0 0 0 0 0.14 0.08 0.13 0.08 0.06 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.12 0 0 0 0 0 0 0 0 0 0.17 0.1 0.05 0.09 0 0.13 0.34 0.46 0.24 0.54 0.56 0.53
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 2 2
Bud 1 1 0 0 0 2 2 1 2 1 1 3 1 2 0 0 0 0 0 0 0 2 2
Bud Neck 0 0 6 3 10 5 13 9 7 9 11 0 0 0 0 0 0 0 1 1 0 1 3
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 6 0 2 0 3 5 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 230 126 196 191 304 280 409 470 185 205 215 283 427 464 95 41 39 123 67 108 30 93 111
Endoplasmic Reticulum 3 2 0 0 0 0 1 0 0 1 0 2 1 2 6 5 1 6 0 4 2 6 9
Endosome 0 0 0 0 0 0 0 0 0 0 1 1 1 1 2 1 0 17 10 6 4 14 16
Golgi 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 1 0 0 0 0 2 0
Mitochondria 37 22 0 7 2 11 12 10 26 41 20 9 3 3 4 1 0 41 14 26 9 20 29
Nucleus 0 1 1 1 0 1 0 4 1 0 2 1 0 2 0 0 0 0 1 0 0 0 1
Nuclear Periphery 3 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 1 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 1 0 1 0 1 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
SpindlePole 0 0 1 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1
Vac/Vac Membrane 20 19 1 0 4 6 0 0 1 0 3 58 47 24 9 0 6 103 86 49 62 187 216
Unique Cell Count 271 157 200 197 309 291 418 479 198 231 234 337 456 485 104 44 45 308 187 206 116 337 406
Labelled Cell Count 294 171 213 202 322 306 440 500 223 260 255 358 482 500 117 49 47 308 187 206 116 337 406


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.6 4.3 4.0 4.4 3.5 3.5 3.9 3.0 3.0 3.3 4.1 4.3 4.3 5.8 5.8 5.7 4.0 3.8 4.3
Std Deviation (1e-4) 0.6 0.6 0.6 0.7 0.5 0.9 0.6 0.9 0.8 0.5 0.6 1.0 1.3 1.2 1.5 1.5 1.9 1.6 1.0 1.7
Intensity Change (Log2) -0.11 0.05 -0.29 -0.31 -0.13 -0.5 -0.53 -0.36 -0.04 -0.0 0.01 0.43 0.44 0.42 -0.11 -0.18 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.458 -1.2179 -1.4216 -1.279 -1.6128 -1.4392 -0.5267 -0.6132 -0.9441 -0.2042 -1.1735 -0.4867 -0.4496 -0.646 -1.1263 -1.1027 -0.5169 -1.0626
Actin 0.0227 0.0014 0.0219 0.0609 0.0006 0.0157 0.0072 0.0005 0.0063 0.0015 0.0015 0.0155 0.0559 0.0004 0.0007 0.0001 0.0489 0.0003
Bud 0.0046 0.0011 0.0005 0.0015 0.0001 0.0003 0.002 0.0011 0.0022 0.0036 0.0005 0.0007 0.0032 0.0005 0.0031 0.0008 0.0006 0.0003
Bud Neck 0.0138 0.0003 0.0012 0.0006 0.0002 0.0027 0.0011 0.0002 0.0006 0.0009 0.0006 0.0046 0.0013 0.0002 0.0003 0.0004 0.0012 0.0023
Bud Periphery 0.0083 0.002 0.0006 0.0019 0.0001 0.0004 0.0058 0.0026 0.0025 0.0186 0.0006 0.001 0.0028 0.0004 0.0023 0.0009 0.0006 0.0003
Bud Site 0.0059 0.0032 0.0026 0.0014 0.0001 0.0011 0.0071 0.002 0.0041 0.0008 0.0005 0.0007 0.0121 0.001 0.0016 0.0004 0.0023 0.0004
Cell Periphery 0.0008 0.0004 0.0003 0.0003 0.0001 0.0001 0.0008 0.0004 0.0005 0.0004 0.0002 0.0002 0.0007 0.0003 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.0944 0.1539 0.1671 0.0883 0.1332 0.1876 0.1435 0.2403 0.1877 0.1605 0.1948 0.2488 0.1266 0.2368 0.1906 0.1436 0.1679 0.2543
Cytoplasmic Foci 0.0637 0.0575 0.0795 0.0827 0.0361 0.0903 0.1148 0.0583 0.0994 0.1118 0.0677 0.0785 0.0619 0.0455 0.0608 0.0727 0.057 0.0954
Eisosomes 0.0014 0.0003 0.0003 0.0003 0.0004 0.0002 0.0003 0.0002 0.0002 0.0001 0.0001 0.0001 0.001 0.0001 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0035 0.0034 0.0043 0.0058 0.0011 0.0017 0.0081 0.0056 0.0047 0.0029 0.003 0.0031 0.0055 0.0054 0.0037 0.0019 0.0006 0.0019
Endosome 0.1474 0.0735 0.1376 0.2189 0.1559 0.1786 0.2222 0.1718 0.2344 0.3306 0.2773 0.2727 0.1785 0.1384 0.183 0.2398 0.1919 0.2614
Golgi 0.0044 0.0013 0.0043 0.009 0.001 0.0029 0.0146 0.0024 0.0069 0.0191 0.0143 0.0107 0.0175 0.0018 0.0071 0.0019 0.0283 0.0049
Lipid Particles 0.0112 0.0074 0.0133 0.0413 0.0141 0.015 0.0407 0.0055 0.0058 0.0745 0.0269 0.0049 0.0287 0.0096 0.0059 0.0039 0.0211 0.0107
Mitochondria 0.0043 0.0015 0.0039 0.0145 0.0025 0.0027 0.0237 0.0032 0.0121 0.0318 0.0224 0.0243 0.0168 0.0017 0.0077 0.0028 0.0103 0.0165
None 0.4906 0.6151 0.4616 0.3326 0.4749 0.4121 0.2215 0.3629 0.2422 0.0764 0.1457 0.1504 0.2794 0.4106 0.385 0.265 0.3107 0.1831
Nuclear Periphery 0.0044 0.0026 0.0046 0.0105 0.0033 0.0013 0.0086 0.0039 0.0066 0.0021 0.0023 0.0051 0.0053 0.0048 0.0118 0.0029 0.0006 0.0019
Nucleolus 0.0022 0.0008 0.001 0.0036 0.0007 0.0007 0.0042 0.0008 0.0014 0.001 0.0008 0.0011 0.002 0.0007 0.0008 0.0011 0.0002 0.0007
Nucleus 0.016 0.0025 0.0028 0.0078 0.0017 0.0029 0.0083 0.0037 0.0053 0.004 0.0044 0.0059 0.0045 0.0032 0.0037 0.0036 0.0008 0.0029
Peroxisomes 0.0082 0.0014 0.0111 0.0381 0.0025 0.0079 0.0177 0.0009 0.0115 0.0122 0.0257 0.0047 0.0095 0.0031 0.0027 0.0027 0.0102 0.0036
Punctate Nuclear 0.0061 0.0017 0.0027 0.0068 0.0024 0.0025 0.0161 0.0013 0.002 0.0011 0.0007 0.0042 0.004 0.0014 0.0025 0.0016 0.0003 0.0012
Vacuole 0.0796 0.0629 0.0693 0.062 0.1487 0.0606 0.1172 0.1165 0.1375 0.1204 0.1788 0.1343 0.1712 0.1187 0.1087 0.2116 0.1231 0.1376
Vacuole Periphery 0.0064 0.0058 0.0093 0.0112 0.0204 0.0127 0.0144 0.0159 0.0263 0.0255 0.0314 0.0287 0.0118 0.0154 0.0177 0.042 0.023 0.0202

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.0489 18.4124 11.7109 15.839 20.3465 12.2533 18.2843 21.3905 19.5984 23.7257
Translational Efficiency 0.5138 0.6027 0.7418 0.4025 0.3864 0.7987 0.5294 0.3443 0.3756 0.3648

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
482 789 343 1193 1792 1360 156 585 2274 2149 499 1778

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 559.26 704.70 916.80 814.81 649.74 719.06 933.36 790.78 630.56 713.79 921.98 806.90
Standard Deviation 60.95 100.57 119.56 104.71 76.24 100.44 106.01 97.84 82.07 100.73 115.75 103.12
Intensity Change Log 2 0.333490 0.713088 0.542945 0.146250 0.522571 0.283414 0.235892 0.613836 0.409289

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000029 0.001080 0.000956 0.000669 0.000195 0.000287 0.000247 0.000389 0.000160 0.000578 0.000735 0.000577
Bud Neck 0.004017 0.031349 0.005692 0.005243 0.021220 0.032972 0.001702 0.003395 0.017574 0.032376 0.004445 0.004635
Bud Site 0.000773 0.011911 0.016035 0.034128 0.005419 0.013411 0.001924 0.008851 0.004435 0.012861 0.011624 0.025811
Cell Periphery 0.000126 0.000127 0.000242 0.000141 0.000125 0.000170 0.000231 0.000177 0.000125 0.000155 0.000239 0.000153
Cytoplasm 0.525686* 0.397862* 0.212086* 0.452798* 0.390560* 0.356617* 0.247748* 0.508428* 0.419201* 0.371760* 0.223235* 0.471101*
Cytoplasmic Foci 0.171433 0.243769* 0.006757 0.034491 0.277510* 0.251608* 0.043573 0.059291 0.255026* 0.248730* 0.018266 0.042650
Eisosomes 0.000147 0.000117 0.000084 0.000053 0.000206 0.000170 0.000136 0.000056 0.000193 0.000150 0.000100 0.000054
Endoplasmic Reticulum 0.000371 0.000921 0.012452 0.001810 0.001541 0.000733 0.005139 0.001602 0.001293 0.000802 0.010166 0.001742
Endosome 0.008913 0.037687 0.004278 0.011435 0.036000 0.043860 0.011539 0.009754 0.030258 0.041594 0.006548 0.010882
Golgi 0.000739 0.003551 0.000174 0.003220 0.004542 0.005877 0.002125 0.000777 0.003736 0.005023 0.000784 0.002416
Lipid Particles 0.011194 0.006042 0.002975 0.001842 0.014738 0.009394 0.005085 0.002178 0.013986 0.008163 0.003635 0.001952
Mitochondria 0.001375 0.005323 0.001234 0.007345 0.006530 0.010006 0.000242 0.000322 0.005437 0.008287 0.000924 0.005034
Mitotic Spindle 0.000045 0.010390 0.006349 0.032354 0.000571 0.006237 0.000275 0.008390 0.000459 0.007762 0.004450 0.024469
None 0.009865 0.002347 0.004464 0.003359 0.001455 0.001641 0.003988 0.007388 0.003237 0.001900 0.004315 0.004685
Nuclear Periphery 0.000530 0.000651 0.002853 0.001738 0.000569 0.000631 0.002832 0.001274 0.000561 0.000638 0.002846 0.001586
Nuclear Periphery Foci 0.000384 0.000630 0.003351 0.001607 0.000353 0.000302 0.002283 0.001640 0.000360 0.000422 0.003017 0.001618
Nucleolus 0.002918 0.002614 0.001086 0.000647 0.001227 0.001453 0.001001 0.000707 0.001585 0.001880 0.001059 0.000667
Nucleus 0.220538* 0.089865 0.632613* 0.202575* 0.100106 0.110164 0.473543* 0.218331* 0.125633 0.102711 0.582884* 0.207759*
Peroxisomes 0.002272 0.014699 0.000643 0.001978 0.006192 0.014459 0.000578 0.001126 0.005361 0.014547 0.000622 0.001698
Vacuole 0.038030 0.136771 0.085098 0.197729 0.128399 0.136145 0.194382 0.165548 0.109244 0.136375 0.119263 0.187141
Vacuole Periphery 0.000613 0.002293 0.000578 0.004838 0.002545 0.003863 0.001428 0.000379 0.002136 0.003287 0.000843 0.003371

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.75 -13.16 -5.07 1.09 6.63 -1.58 -2.25 -1.35 -0.34 0.27 -1.87 -10.65 -3.39 0.85 6.12
Bud Neck -8.68 -2.89 -1.89 8.38 1.92 -4.61 14.94 13.54 13.20 -2.58 -7.01 10.61 12.75 15.37 1.28
Bud Site -9.38 -3.49 -6.64 -1.91 -1.40 -5.05 4.31 -0.58 3.22 -2.46 -7.42 -1.93 -5.05 -0.34 -1.77
Cell Periphery 0.13 -6.35 2.83 1.26 8.27 -0.98 -3.05 -0.12 0.77 2.20 -0.88 -5.19 0.81 1.51 7.27
Cytoplasm 9.08 22.88 12.27 2.30 -14.11 3.61 8.55 -2.14 -4.61 -8.94 6.21 20.95 4.59 -1.30 -16.65
Cytoplasmic Foci -6.48 23.80 21.25 28.91 -11.35 3.53 37.69 41.03 32.44 -1.42 1.46 53.51 51.10 46.22 -6.82
Eisosomes 2.41 3.87 8.00 9.07 6.35 1.60 3.22 8.13 6.47 7.18 2.55 5.48 9.59 8.53 9.29
Endoplasmic Reticulum -5.58 -15.69 -17.00 -10.28 13.32 5.29 -6.30 -4.97 -10.07 4.84 3.72 -15.49 -9.07 -15.77 13.71
Endosome -10.22 3.81 2.65 11.97 -1.09 -2.68 10.02 14.85 14.59 1.13 -4.85 15.96 16.68 19.35 0.26
Golgi -4.26 1.60 -0.81 3.59 -2.79 -1.48 3.44 9.39 6.94 0.65 -1.91 7.72 7.18 7.08 -1.20
Lipid Particles 2.28 3.63 4.33 8.36 5.32 4.57 10.63 15.64 8.19 6.19 6.01 12.59 15.40 10.54 7.92
Mitochondria -4.18 0.79 -1.50 1.63 -1.82 -2.30 6.77 6.73 8.56 -0.81 -2.49 6.21 3.41 5.92 -1.81
Mitotic Spindle -3.85 -2.77 -6.96 -2.80 -3.49 -3.91 -0.17 -3.10 -0.59 -3.09 -5.41 -2.62 -7.46 -3.63 -4.11
None 3.22 2.67 2.80 -1.94 0.54 -0.49 -1.94 -4.93 -4.62 -2.51 2.20 -0.34 -1.61 -5.21 -1.61
Nuclear Periphery -1.73 -16.05 -17.12 -14.48 3.66 -0.77 -7.92 -12.36 -10.25 3.37 -1.44 -17.48 -20.45 -17.63 5.01
Nuclear Periphery Foci -2.84 -8.79 -5.97 -4.41 6.18 0.78 -6.00 -7.47 -7.87 1.99 -1.28 -10.38 -9.07 -8.70 6.43
Nucleolus 0.23 1.73 2.15 3.78 4.17 -0.89 2.15 3.38 5.59 1.71 -0.81 2.30 3.75 5.92 4.48
Nucleus 12.82 -23.60 -2.20 -18.52 23.52 -1.99 -14.51 -15.39 -13.61 7.51 4.76 -32.82 -19.41 -22.62 22.68
Peroxisomes -7.79 2.90 2.21 9.37 -0.86 -5.85 13.26 10.92 10.12 -0.55 -8.54 13.15 10.74 13.42 -1.00
Vacuole -16.86 -9.66 -30.90 -14.88 -13.71 -1.75 -6.35 -11.02 -9.74 0.55 -7.14 -6.38 -24.71 -19.04 -9.73
Vacuole Periphery -6.07 0.96 -2.08 0.64 -2.38 -2.98 3.14 14.58 8.96 1.02 -3.65 7.31 2.27 4.30 -1.73