Standard name | |
---|---|
Human Ortholog | |
Description | DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; along with Rfa1p, binds to ultrafine anaphase bridges in mitotic cells and prevents accumulation of chromatin bridges by stimulating the Mec1p kinase and suppressing homologous recombination; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 3 |
Bud | 2 | 1 | 0 | 1 | 1 | 1 | 3 | 0 | 9 | 3 | 6 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 6 | 9 | 6 |
Bud Neck | 1 | 1 | 10 | 6 | 6 | 1 | 8 | 14 | 5 | 1 | 3 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 3 | 5 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 1 | 7 | 1 | 6 | 7 | 8 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 46 | 82 | 101 | 52 | 70 | 160 | 133 | 121 | 102 | 100 | 64 | 61 | 72 | 82 | 98 | 164 | 151 | 130 | 44 | 55 | 68 | 39 | 98 | 133 |
Endoplasmic Reticulum | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 12 | 8 | 4 | 1 | 0 | 1 | 0 | 3 | 5 |
Endosome | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 21 | 3 | 8 | 10 | 2 | 0 | 5 | 0 | 1 | 1 | 4 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 0 |
Mitochondria | 35 | 42 | 9 | 31 | 22 | 87 | 112 | 71 | 190 | 211 | 180 | 148 | 47 | 12 | 36 | 8 | 5 | 2 | 11 | 7 | 12 | 14 | 24 | 30 |
Nucleus | 40 | 57 | 65 | 52 | 36 | 61 | 64 | 64 | 60 | 91 | 78 | 82 | 88 | 53 | 69 | 18 | 14 | 28 | 60 | 94 | 73 | 26 | 17 | 9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 4 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 4 | 0 | 2 | 1 | 5 | 5 | 4 | 2 | 2 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 7 | 14 | 5 | 2 | 5 | 9 |
Vac/Vac Membrane | 35 | 53 | 11 | 4 | 13 | 17 | 8 | 16 | 6 | 11 | 8 | 4 | 56 | 63 | 55 | 108 | 101 | 54 | 50 | 80 | 84 | 61 | 106 | 80 |
Unique Cell Count | 138 | 206 | 189 | 130 | 137 | 299 | 266 | 257 | 285 | 328 | 254 | 244 | 235 | 214 | 230 | 262 | 246 | 196 | 185 | 277 | 269 | 162 | 281 | 301 |
Labelled Cell Count | 161 | 244 | 198 | 148 | 150 | 333 | 340 | 294 | 382 | 429 | 351 | 326 | 270 | 236 | 264 | 321 | 292 | 222 | 185 | 277 | 269 | 162 | 281 | 301 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.0 | 4.0 | 4.6 | 4.2 | 4.8 | 3.8 | 3.8 | 4.2 | 3.3 | 3.3 | 3.1 | 3.6 | 4.5 | 4.5 | 4.1 | 7.0 | 7.7 | 6.8 | 4.2 | 4.1 | 4.6 |
Std Deviation (1e-4) | 0.7 | 0.8 | 1.0 | 1.2 | 1.4 | 1.3 | 1.2 | 1.4 | 0.8 | 1.1 | 0.9 | 0.9 | 1.5 | 1.3 | 1.3 | 1.5 | 2.0 | 1.7 | 1.8 | 1.6 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.13 | 0.06 | -0.26 | -0.25 | -0.14 | -0.48 | -0.48 | -0.54 | -0.36 | -0.03 | -0.02 | -0.14 | 0.62 | 0.76 | 0.58 | -0.11 | -0.15 | 0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 8.4 | 8.3 | 5.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.6069 | -0.6193 | -0.5326 | -1.2208 | -0.7732 | -0.8288 | 0.0297 | 0.1845 | 0.2518 | -0.3198 | -0.2554 | 0.1699 | -0.9014 | -0.1766 | -0.6635 | -0.3951 | -0.6146 | -0.6176 |
Actin | 0.0653 | 0.0008 | 0.0101 | 0.0006 | 0.0006 | 0.0123 | 0.0262 | 0.0004 | 0.0101 | 0.0377 | 0.0006 | 0.0171 | 0.0003 | 0.0043 | 0.001 | 0.0003 | 0.0004 | 0.0004 |
Bud | 0.0012 | 0.0004 | 0.0009 | 0.0001 | 0 | 0.0014 | 0.001 | 0.0002 | 0.0003 | 0.0004 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0 | 0 | 0.0001 |
Bud Neck | 0.0024 | 0.0008 | 0.005 | 0.0002 | 0.0006 | 0.0066 | 0.0033 | 0.0022 | 0.0015 | 0.0011 | 0.0032 | 0.005 | 0.0004 | 0.0003 | 0.0007 | 0.0005 | 0.0007 | 0.0033 |
Bud Periphery | 0.0016 | 0.0005 | 0.0013 | 0.0001 | 0 | 0.0018 | 0.002 | 0.0002 | 0.0003 | 0.0004 | 0.0008 | 0.0003 | 0.0001 | 0.0001 | 0.0005 | 0 | 0 | 0.0001 |
Bud Site | 0.0102 | 0.003 | 0.0041 | 0.0002 | 0.0027 | 0.0006 | 0.0056 | 0.0027 | 0.0088 | 0.0012 | 0.0052 | 0.0004 | 0.0003 | 0.0011 | 0.0028 | 0.0001 | 0.0001 | 0.0005 |
Cell Periphery | 0.0007 | 0.0002 | 0.0002 | 0 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.1494 | 0.255 | 0.1555 | 0.1482 | 0.1564 | 0.1498 | 0.1537 | 0.2616 | 0.1459 | 0.1317 | 0.1354 | 0.1284 | 0.1294 | 0.1169 | 0.0945 | 0.0724 | 0.0997 | 0.097 |
Cytoplasmic Foci | 0.0336 | 0.0047 | 0.0258 | 0.0084 | 0.1006 | 0.0131 | 0.0277 | 0.0042 | 0.0109 | 0.0054 | 0.0294 | 0.0072 | 0.0069 | 0.0053 | 0.006 | 0.0015 | 0.0117 | 0.0036 |
Eisosomes | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0071 | 0.0094 | 0.0069 | 0.0032 | 0.0453 | 0.0073 | 0.0082 | 0.0088 | 0.0041 | 0.0023 | 0.0019 | 0.002 | 0.0036 | 0.0028 | 0.0027 | 0.0009 | 0.0015 | 0.0014 |
Endosome | 0.0482 | 0.0063 | 0.0326 | 0.0055 | 0.0042 | 0.0182 | 0.0404 | 0.0042 | 0.0121 | 0.0034 | 0.0622 | 0.0087 | 0.0076 | 0.0041 | 0.0067 | 0.0011 | 0.0037 | 0.0021 |
Golgi | 0.0299 | 0.0003 | 0.0137 | 0.0007 | 0.0011 | 0.001 | 0.0149 | 0.0001 | 0.0092 | 0.0036 | 0.0319 | 0.0091 | 0.0002 | 0.0009 | 0.0002 | 0 | 0.0001 | 0.0001 |
Lipid Particles | 0.0299 | 0.001 | 0.0069 | 0.0093 | 0.0102 | 0.0064 | 0.0188 | 0.0006 | 0.0081 | 0.0017 | 0.0291 | 0.0071 | 0.0015 | 0.001 | 0.0011 | 0.0002 | 0.0006 | 0.0003 |
Mitochondria | 0.027 | 0.0056 | 0.0098 | 0.1042 | 0.0003 | 0.0048 | 0.0073 | 0.0006 | 0.0037 | 0.0015 | 0.0054 | 0.011 | 0.0008 | 0.0008 | 0.0008 | 0.0007 | 0.0006 | 0.0004 |
None | 0.1662 | 0.2309 | 0.2673 | 0.4324 | 0.3836 | 0.0868 | 0.1579 | 0.1438 | 0.1593 | 0.1614 | 0.1636 | 0.0903 | 0.2099 | 0.1924 | 0.1845 | 0.2804 | 0.2354 | 0.137 |
Nuclear Periphery | 0.0386 | 0.0361 | 0.0249 | 0.022 | 0.0382 | 0.0384 | 0.0477 | 0.03 | 0.0313 | 0.0188 | 0.016 | 0.0157 | 0.0296 | 0.016 | 0.0273 | 0.011 | 0.0112 | 0.0122 |
Nucleolus | 0.0039 | 0.0059 | 0.0075 | 0.0057 | 0.0011 | 0.0061 | 0.0059 | 0.0038 | 0.0054 | 0.0052 | 0.0117 | 0.0064 | 0.0048 | 0.0039 | 0.0064 | 0.0077 | 0.0055 | 0.0093 |
Nucleus | 0.3017 | 0.3943 | 0.3463 | 0.1765 | 0.2157 | 0.5912 | 0.4215 | 0.4957 | 0.5509 | 0.5737 | 0.4575 | 0.6295 | 0.558 | 0.6176 | 0.6159 | 0.5645 | 0.5374 | 0.6693 |
Peroxisomes | 0.0076 | 0.0003 | 0.0191 | 0.0199 | 0.0049 | 0.0005 | 0.0186 | 0.0001 | 0.0076 | 0.0035 | 0.0101 | 0.004 | 0.0003 | 0.0039 | 0.0002 | 0.0001 | 0.0003 | 0.0002 |
Punctate Nuclear | 0.0554 | 0.0389 | 0.0537 | 0.0607 | 0.0321 | 0.046 | 0.0289 | 0.0364 | 0.027 | 0.0453 | 0.0282 | 0.0549 | 0.0417 | 0.0252 | 0.0462 | 0.0577 | 0.0901 | 0.0615 |
Vacuole | 0.0121 | 0.0049 | 0.007 | 0.0013 | 0.0017 | 0.0055 | 0.0068 | 0.0035 | 0.0024 | 0.0012 | 0.006 | 0.0022 | 0.0033 | 0.0029 | 0.0015 | 0.0005 | 0.0007 | 0.0008 |
Vacuole Periphery | 0.0074 | 0.0008 | 0.0013 | 0.0007 | 0.0006 | 0.002 | 0.0025 | 0.0006 | 0.0009 | 0.0003 | 0.0011 | 0.0005 | 0.0007 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.1349 | 6.0171 | 4.465 | 9.33 | 6.2336 | 2.8831 | 6.0839 | 9.5323 | 6.4302 | 10.6078 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2435 | 1563 | 163 | 1620 | 1861 | 1904 | 1867 | 1476 | 4296 | 3467 | 2030 | 3096 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 645.67 | 748.47 | 947.68 | 894.66 | 658.69 | 740.49 | 862.10 | 868.53 | 651.31 | 744.09 | 868.97 | 882.20 |
Standard Deviation | 65.82 | 97.36 | 99.84 | 107.92 | 77.64 | 88.72 | 101.22 | 117.24 | 71.47 | 92.80 | 103.75 | 113.21 |
Intensity Change Log 2 | — | 0.213147 | 0.553603 | 0.470543 | — | 0.168881 | 0.388256 | 0.398976 | — | 0.190963 | 0.472472 | 0.434846 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000020 | 0.000322 | 0.000989 | 0.000884 | 0.000046 | 0.000372 | 0.000208 | 0.000434 | 0.000031 | 0.000349 | 0.000271 | 0.000669 |
Bud Neck | 0.006187 | 0.007968 | 0.003321 | 0.017040 | 0.007006 | 0.011680 | 0.006059 | 0.008891 | 0.006542 | 0.010006 | 0.005839 | 0.013155 |
Bud Site | 0.001002 | 0.005381 | 0.001106 | 0.012278 | 0.001428 | 0.006330 | 0.001851 | 0.008053 | 0.001187 | 0.005902 | 0.001791 | 0.010264 |
Cell Periphery | 0.000100 | 0.000125 | 0.000077 | 0.000100 | 0.000122 | 0.000169 | 0.000080 | 0.000086 | 0.000109 | 0.000149 | 0.000080 | 0.000093 |
Cytoplasm | 0.370832* | 0.252998* | 0.004686 | 0.039584 | 0.328801* | 0.234746* | 0.019470 | 0.018859 | 0.352625* | 0.242974* | 0.018283 | 0.029704 |
Cytoplasmic Foci | 0.055134 | 0.054383 | 0.000315 | 0.001403 | 0.090779 | 0.074727 | 0.000330 | 0.000294 | 0.070575 | 0.065555 | 0.000329 | 0.000875 |
Eisosomes | 0.000041 | 0.000049 | 0.000054 | 0.000021 | 0.000060 | 0.000069 | 0.000015 | 0.000019 | 0.000049 | 0.000060 | 0.000018 | 0.000020 |
Endoplasmic Reticulum | 0.000350 | 0.001476 | 0.012037 | 0.007765 | 0.000976 | 0.001449 | 0.004243 | 0.007847 | 0.000621 | 0.001461 | 0.004868 | 0.007804 |
Endosome | 0.001520 | 0.004745 | 0.000537 | 0.003007 | 0.003068 | 0.004180 | 0.000439 | 0.001020 | 0.002191 | 0.004435 | 0.000446 | 0.002060 |
Golgi | 0.000459 | 0.001563 | 0.000117 | 0.002189 | 0.000583 | 0.002186 | 0.000030 | 0.000454 | 0.000513 | 0.001905 | 0.000037 | 0.001362 |
Lipid Particles | 0.003299 | 0.004136 | 0.000791 | 0.000405 | 0.006798 | 0.008142 | 0.000199 | 0.000227 | 0.004815 | 0.006336 | 0.000247 | 0.000320 |
Mitochondria | 0.002662 | 0.006564 | 0.001224 | 0.004095 | 0.003383 | 0.011008 | 0.000760 | 0.001331 | 0.002975 | 0.009005 | 0.000797 | 0.002778 |
Mitotic Spindle | 0.000203 | 0.004309 | 0.008002 | 0.019042 | 0.000854 | 0.003710 | 0.001548 | 0.007468 | 0.000485 | 0.003980 | 0.002066 | 0.013524 |
None | 0.007978 | 0.011138 | 0.000942 | 0.004217 | 0.006798 | 0.006657 | 0.003298 | 0.004628 | 0.007467 | 0.008677 | 0.003109 | 0.004413 |
Nuclear Periphery | 0.000759 | 0.001347 | 0.001055 | 0.003572 | 0.001165 | 0.001654 | 0.001141 | 0.002035 | 0.000935 | 0.001516 | 0.001134 | 0.002839 |
Nuclear Periphery Foci | 0.000209 | 0.000572 | 0.000296 | 0.000493 | 0.000430 | 0.000567 | 0.000161 | 0.000666 | 0.000305 | 0.000569 | 0.000172 | 0.000576 |
Nucleolus | 0.001916 | 0.004932 | 0.002493 | 0.001538 | 0.002735 | 0.004865 | 0.000956 | 0.001050 | 0.002270 | 0.004895 | 0.001080 | 0.001306 |
Nucleus | 0.539401* | 0.616254* | 0.950296* | 0.865920* | 0.529935* | 0.600846* | 0.954203* | 0.928933* | 0.535300* | 0.607792* | 0.953890* | 0.895961* |
Peroxisomes | 0.001336 | 0.006229 | 0.000945 | 0.000270 | 0.002720 | 0.009155 | 0.000144 | 0.000198 | 0.001935 | 0.007836 | 0.000208 | 0.000236 |
Vacuole | 0.006390 | 0.014291 | 0.010523 | 0.014195 | 0.011974 | 0.016408 | 0.004754 | 0.007319 | 0.008809 | 0.015454 | 0.005217 | 0.010917 |
Vacuole Periphery | 0.000202 | 0.001220 | 0.000193 | 0.001982 | 0.000339 | 0.001081 | 0.000112 | 0.000187 | 0.000261 | 0.001144 | 0.000118 | 0.001126 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.80 | -8.91 | -7.55 | -4.19 | 2.06 | -4.03 | -11.96 | -17.58 | -2.85 | -9.33 | -5.57 | -15.17 | -11.70 | -4.97 | -6.20 |
Bud Neck | -1.91 | 1.14 | -8.47 | -6.98 | -8.83 | -3.85 | -0.46 | -5.53 | -1.87 | -4.83 | -4.36 | -0.75 | -10.07 | -6.14 | -7.98 |
Bud Site | -4.62 | -1.91 | -6.28 | -1.96 | -4.68 | -4.56 | -0.68 | -3.68 | -0.37 | -3.28 | -6.63 | -1.41 | -6.91 | -1.54 | -5.41 |
Cell Periphery | -2.12 | -0.29 | 0.22 | 2.56 | 0.38 | -1.71 | 2.41 | 1.91 | 3.51 | -1.17 | -2.64 | 2.37 | 1.75 | 4.18 | -1.40 |
Cytoplasm | 14.58 | 67.52* | 61.12* | 32.18 | -10.55 | 12.04 | 50.94 | 51.57 | 39.90 | 0.72 | 19.71 | 80.97 | 80.16* | 50.36 | -4.00 |
Cytoplasmic Foci | 0.04 | 23.96 | 23.74 | 18.36 | -2.11 | 3.37 | 27.91 | 27.94 | 24.40 | 0.67 | 1.47 | 36.39 | 36.30 | 30.41 | -1.23 |
Eisosomes | -3.28 | -3.77 | 3.19 | 5.68 | 4.89 | -2.53 | 16.44 | 12.36 | 13.30 | -5.93 | -4.94 | 16.21 | 11.16 | 13.77 | -6.64 |
Endoplasmic Reticulum | -11.83 | -10.72 | -25.29 | -21.94 | 3.30 | -2.69 | -14.23 | -20.01 | -19.79 | -8.49 | -8.70 | -19.66 | -32.40 | -29.51 | -9.73 |
Endosome | -5.26 | 3.05 | -0.37 | 4.54 | -2.35 | -1.91 | 5.41 | 4.03 | 6.68 | -1.72 | -5.45 | 6.76 | 2.70 | 7.58 | -3.47 |
Golgi | -2.65 | 1.85 | -0.94 | 1.22 | -1.86 | -2.72 | 4.97 | 1.21 | 3.07 | -1.22 | -3.68 | 4.23 | -0.32 | 3.03 | -2.38 |
Lipid Particles | -2.06 | 8.19 | 10.35 | 10.09 | 3.76 | -1.47 | 12.21 | 12.07 | 10.15 | -1.91 | -2.97 | 16.15 | 15.74 | 13.09 | -3.14 |
Mitochondria | -3.35 | 1.94 | -0.70 | 3.01 | -2.95 | -5.80 | 4.79 | 3.01 | 7.60 | -4.52 | -6.75 | 5.39 | 1.14 | 7.69 | -5.80 |
Mitotic Spindle | -4.52 | -1.33 | -6.84 | -4.74 | -1.89 | -3.25 | -1.47 | -4.83 | -2.96 | -4.10 | -5.56 | -2.52 | -8.40 | -5.58 | -6.58 |
None | -2.36 | 11.78 | 4.51 | 4.92 | -10.30 | 0.03 | 4.38 | 2.88 | 2.67 | -1.72 | -1.62 | 6.81 | 5.27 | 5.50 | -2.89 |
Nuclear Periphery | -5.94 | -4.43 | -9.62 | -8.07 | -7.50 | -2.98 | -3.79 | -9.09 | -6.18 | -7.63 | -5.91 | -8.12 | -12.75 | -9.90 | -10.26 |
Nuclear Periphery Foci | -2.09 | -0.97 | -2.46 | -1.76 | -2.14 | -1.88 | 2.10 | -2.22 | -1.82 | -2.50 | -2.76 | 1.13 | -3.26 | -2.50 | -3.56 |
Nucleolus | -4.11 | -0.21 | -2.08 | 1.93 | -1.87 | -3.26 | 2.86 | -0.02 | 3.18 | -2.74 | -5.24 | 3.00 | -1.67 | 3.50 | -4.02 |
Nucleus | -7.53 | -32.63 | -41.31 | -25.41 | 6.03 | -6.83 | -56.73 | -46.99* | -37.10 | 7.02 | -10.04 | -79.55 | -62.35 | -43.46 | 13.77 |
Peroxisomes | -6.14 | 1.27 | 5.71 | 7.88 | 1.16 | -6.63 | 9.58 | 9.58 | 9.72 | -0.40 | -9.22 | 10.12 | 10.80 | 12.46 | 0.08 |
Vacuole | -8.11 | -2.96 | -11.11 | -5.39 | -0.79 | -4.05 | 2.51 | -1.41 | 1.83 | -3.08 | -9.01 | -1.24 | -10.07 | -3.23 | -7.16 |
Vacuole Periphery | -2.63 | -0.65 | -1.65 | 0.65 | -1.53 | -3.87 | 3.93 | 3.38 | 4.77 | -1.01 | -4.34 | 2.87 | -1.29 | 2.12 | -1.88 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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