Standard name
Human Ortholog
Description DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; along with Rfa1p, binds to ultrafine anaphase bridges in mitotic cells and prevents accumulation of chromatin bridges by stimulating the Mec1p kinase and suppressing homologous recombination; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0.05 0 0 0 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.33 0.4 0.53 0.4 0.51 0.54 0.5 0.47 0.36 0.3 0.25 0.25 0.31 0.38 0.43 0.63 0.61 0.66 0.24 0.2 0.25 0.24 0.35 0.44
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.25 0.2 0.05 0.24 0.16 0.29 0.42 0.28 0.67 0.64 0.71 0.61 0.2 0.06 0.16 0 0 0 0.06 0 0.05 0.09 0.09 0.1
Nucleus 0.29 0.28 0.34 0.4 0.26 0.2 0.24 0.25 0.21 0.28 0.31 0.34 0.37 0.25 0.3 0.07 0.06 0.14 0.33 0.34 0.27 0.17 0.06 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Vac/Vac Membrane 0.25 0.26 0.06 0 0.09 0.06 0 0.06 0 0 0 0 0.24 0.29 0.24 0.41 0.41 0.28 0.27 0.29 0.32 0.38 0.38 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 3
Bud 2 1 0 1 1 1 3 0 9 3 6 6 1 0 0 0 0 0 1 3 2 6 9 6
Bud Neck 1 1 10 6 6 1 8 14 5 1 3 12 0 0 0 0 0 0 0 3 3 0 3 5
Bud Site 0 1 0 0 0 1 7 1 6 7 8 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 0 0 0 0 2 0 0 0 0 0 0 0 2 2 0 0 0 0 0 0 1
Cytoplasm 46 82 101 52 70 160 133 121 102 100 64 61 72 82 98 164 151 130 44 55 68 39 98 133
Endoplasmic Reticulum 2 0 0 0 0 0 0 0 0 0 1 0 1 0 3 12 8 4 1 0 1 0 3 5
Endosome 0 1 0 0 1 0 0 0 0 0 0 0 2 21 3 8 10 2 0 5 0 1 1 4
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 0 0 1 0
Mitochondria 35 42 9 31 22 87 112 71 190 211 180 148 47 12 36 8 5 2 11 7 12 14 24 30
Nucleus 40 57 65 52 36 61 64 64 60 91 78 82 88 53 69 18 14 28 60 94 73 26 17 9
Nuclear Periphery 0 0 0 0 0 0 0 1 1 3 1 1 0 0 0 1 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 2 4 0 0 1 1 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 4 0 2 1 5 5 4 2 2 2 3 1 0 0 0 0 0 7 14 5 2 5 9
Vac/Vac Membrane 35 53 11 4 13 17 8 16 6 11 8 4 56 63 55 108 101 54 50 80 84 61 106 80
Unique Cell Count 138 206 189 130 137 299 266 257 285 328 254 244 235 214 230 262 246 196 185 277 269 162 281 301
Labelled Cell Count 161 244 198 148 150 333 340 294 382 429 351 326 270 236 264 321 292 222 185 277 269 162 281 301


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.0 4.6 4.2 4.8 3.8 3.8 4.2 3.3 3.3 3.1 3.6 4.5 4.5 4.1 7.0 7.7 6.8 4.2 4.1 4.6
Std Deviation (1e-4) 0.7 0.8 1.0 1.2 1.4 1.3 1.2 1.4 0.8 1.1 0.9 0.9 1.5 1.3 1.3 1.5 2.0 1.7 1.8 1.6 1.9
Intensity Change (Log2) – – – -0.13 0.06 -0.26 -0.25 -0.14 -0.48 -0.48 -0.54 -0.36 -0.03 -0.02 -0.14 0.62 0.76 0.58 -0.11 -0.15 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 8.4 8.3 5.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6069 -0.6193 -0.5326 -1.2208 -0.7732 -0.8288 0.0297 0.1845 0.2518 -0.3198 -0.2554 0.1699 -0.9014 -0.1766 -0.6635 -0.3951 -0.6146 -0.6176
Actin 0.0653 0.0008 0.0101 0.0006 0.0006 0.0123 0.0262 0.0004 0.0101 0.0377 0.0006 0.0171 0.0003 0.0043 0.001 0.0003 0.0004 0.0004
Bud 0.0012 0.0004 0.0009 0.0001 0 0.0014 0.001 0.0002 0.0003 0.0004 0.0006 0.0002 0.0001 0.0001 0.0005 0 0 0.0001
Bud Neck 0.0024 0.0008 0.005 0.0002 0.0006 0.0066 0.0033 0.0022 0.0015 0.0011 0.0032 0.005 0.0004 0.0003 0.0007 0.0005 0.0007 0.0033
Bud Periphery 0.0016 0.0005 0.0013 0.0001 0 0.0018 0.002 0.0002 0.0003 0.0004 0.0008 0.0003 0.0001 0.0001 0.0005 0 0 0.0001
Bud Site 0.0102 0.003 0.0041 0.0002 0.0027 0.0006 0.0056 0.0027 0.0088 0.0012 0.0052 0.0004 0.0003 0.0011 0.0028 0.0001 0.0001 0.0005
Cell Periphery 0.0007 0.0002 0.0002 0 0 0.0001 0.0006 0.0001 0.0002 0 0.0001 0.0001 0 0.0001 0 0 0 0
Cytoplasm 0.1494 0.255 0.1555 0.1482 0.1564 0.1498 0.1537 0.2616 0.1459 0.1317 0.1354 0.1284 0.1294 0.1169 0.0945 0.0724 0.0997 0.097
Cytoplasmic Foci 0.0336 0.0047 0.0258 0.0084 0.1006 0.0131 0.0277 0.0042 0.0109 0.0054 0.0294 0.0072 0.0069 0.0053 0.006 0.0015 0.0117 0.0036
Eisosomes 0.0007 0.0001 0.0002 0.0002 0.0001 0.0001 0.0005 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0071 0.0094 0.0069 0.0032 0.0453 0.0073 0.0082 0.0088 0.0041 0.0023 0.0019 0.002 0.0036 0.0028 0.0027 0.0009 0.0015 0.0014
Endosome 0.0482 0.0063 0.0326 0.0055 0.0042 0.0182 0.0404 0.0042 0.0121 0.0034 0.0622 0.0087 0.0076 0.0041 0.0067 0.0011 0.0037 0.0021
Golgi 0.0299 0.0003 0.0137 0.0007 0.0011 0.001 0.0149 0.0001 0.0092 0.0036 0.0319 0.0091 0.0002 0.0009 0.0002 0 0.0001 0.0001
Lipid Particles 0.0299 0.001 0.0069 0.0093 0.0102 0.0064 0.0188 0.0006 0.0081 0.0017 0.0291 0.0071 0.0015 0.001 0.0011 0.0002 0.0006 0.0003
Mitochondria 0.027 0.0056 0.0098 0.1042 0.0003 0.0048 0.0073 0.0006 0.0037 0.0015 0.0054 0.011 0.0008 0.0008 0.0008 0.0007 0.0006 0.0004
None 0.1662 0.2309 0.2673 0.4324 0.3836 0.0868 0.1579 0.1438 0.1593 0.1614 0.1636 0.0903 0.2099 0.1924 0.1845 0.2804 0.2354 0.137
Nuclear Periphery 0.0386 0.0361 0.0249 0.022 0.0382 0.0384 0.0477 0.03 0.0313 0.0188 0.016 0.0157 0.0296 0.016 0.0273 0.011 0.0112 0.0122
Nucleolus 0.0039 0.0059 0.0075 0.0057 0.0011 0.0061 0.0059 0.0038 0.0054 0.0052 0.0117 0.0064 0.0048 0.0039 0.0064 0.0077 0.0055 0.0093
Nucleus 0.3017 0.3943 0.3463 0.1765 0.2157 0.5912 0.4215 0.4957 0.5509 0.5737 0.4575 0.6295 0.558 0.6176 0.6159 0.5645 0.5374 0.6693
Peroxisomes 0.0076 0.0003 0.0191 0.0199 0.0049 0.0005 0.0186 0.0001 0.0076 0.0035 0.0101 0.004 0.0003 0.0039 0.0002 0.0001 0.0003 0.0002
Punctate Nuclear 0.0554 0.0389 0.0537 0.0607 0.0321 0.046 0.0289 0.0364 0.027 0.0453 0.0282 0.0549 0.0417 0.0252 0.0462 0.0577 0.0901 0.0615
Vacuole 0.0121 0.0049 0.007 0.0013 0.0017 0.0055 0.0068 0.0035 0.0024 0.0012 0.006 0.0022 0.0033 0.0029 0.0015 0.0005 0.0007 0.0008
Vacuole Periphery 0.0074 0.0008 0.0013 0.0007 0.0006 0.002 0.0025 0.0006 0.0009 0.0003 0.0011 0.0005 0.0007 0.0003 0.0004 0.0002 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.1349 6.0171 4.465 9.33 6.2336 2.8831 6.0839 9.5323 6.4302 10.6078
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; along with Rfa1p, binds to ultrafine anaphase bridges in mitotic cells and prevents accumulation of chromatin bridges by stimulating the Mec1p kinase and suppressing homologous recombination; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
Localization
Cell Percentages nucleus (27%), cytoplasm (8%), mixed (50%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Dpb11

Dpb11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dpb11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available