Standard name | |
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Description | DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; along with Rfa1p, binds to ultrafine anaphase bridges in mitotic cells and prevents accumulation of chromatin bridges by stimulating the Mec1p kinase and suppressing homologous recombination; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 3 |
Bud | 2 | 1 | 0 | 1 | 1 | 1 | 3 | 0 | 9 | 3 | 6 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 6 | 9 | 6 |
Bud Neck | 1 | 1 | 10 | 6 | 6 | 1 | 8 | 14 | 5 | 1 | 3 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 3 | 5 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 1 | 7 | 1 | 6 | 7 | 8 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 46 | 82 | 101 | 52 | 70 | 160 | 133 | 121 | 102 | 100 | 64 | 61 | 72 | 82 | 98 | 164 | 151 | 130 | 44 | 55 | 68 | 39 | 98 | 133 |
Endoplasmic Reticulum | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 12 | 8 | 4 | 1 | 0 | 1 | 0 | 3 | 5 |
Endosome | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 21 | 3 | 8 | 10 | 2 | 0 | 5 | 0 | 1 | 1 | 4 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 0 |
Mitochondria | 35 | 42 | 9 | 31 | 22 | 87 | 112 | 71 | 190 | 211 | 180 | 148 | 47 | 12 | 36 | 8 | 5 | 2 | 11 | 7 | 12 | 14 | 24 | 30 |
Nucleus | 40 | 57 | 65 | 52 | 36 | 61 | 64 | 64 | 60 | 91 | 78 | 82 | 88 | 53 | 69 | 18 | 14 | 28 | 60 | 94 | 73 | 26 | 17 | 9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 4 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 4 | 0 | 2 | 1 | 5 | 5 | 4 | 2 | 2 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 7 | 14 | 5 | 2 | 5 | 9 |
Vac/Vac Membrane | 35 | 53 | 11 | 4 | 13 | 17 | 8 | 16 | 6 | 11 | 8 | 4 | 56 | 63 | 55 | 108 | 101 | 54 | 50 | 80 | 84 | 61 | 106 | 80 |
Unique Cell Count | 138 | 206 | 189 | 130 | 137 | 299 | 266 | 257 | 285 | 328 | 254 | 244 | 235 | 214 | 230 | 262 | 246 | 196 | 185 | 277 | 269 | 162 | 281 | 301 |
Labelled Cell Count | 161 | 244 | 198 | 148 | 150 | 333 | 340 | 294 | 382 | 429 | 351 | 326 | 270 | 236 | 264 | 321 | 292 | 222 | 185 | 277 | 269 | 162 | 281 | 301 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.0 | 4.0 | 4.6 | 4.2 | 4.8 | 3.8 | 3.8 | 4.2 | 3.3 | 3.3 | 3.1 | 3.6 | 4.5 | 4.5 | 4.1 | 7.0 | 7.7 | 6.8 | 4.2 | 4.1 | 4.6 |
Std Deviation (1e-4) | 0.7 | 0.8 | 1.0 | 1.2 | 1.4 | 1.3 | 1.2 | 1.4 | 0.8 | 1.1 | 0.9 | 0.9 | 1.5 | 1.3 | 1.3 | 1.5 | 2.0 | 1.7 | 1.8 | 1.6 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.13 | 0.06 | -0.26 | -0.25 | -0.14 | -0.48 | -0.48 | -0.54 | -0.36 | -0.03 | -0.02 | -0.14 | 0.62 | 0.76 | 0.58 | -0.11 | -0.15 | 0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 8.4 | 8.3 | 5.7 |