Standard name
Human Ortholog
Description Nuclear 5' to 3' single-stranded RNA exonuclease; involved in RNA metabolism, including rRNA and snoRNA processing, as well as poly (A+) dependent and independent mRNA transcription termination; required for cotranscriptional pre-rRNA cleavage; displaces Cdk1p from elongating transcripts, especially as RNAPII reaches the poly(A) site, negatively regulates phosphorylation of the CTD of RNAPII, and inhibits RNAPII transcriptional elongation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0.07 0.15 0.15 0.1 0.05 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.07 0.06 0.05 0.16 0.15 0.23 0.34 0.33 0.38 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.96 0.95 0.96 0.93 0.87 0.87 0.82 0.78 0.8 0.76 0.92 0.8 0.78 0.79 0.88 0.86 0.94 0.93 0.94 0.87 0.75 0.57
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.05 0.11 0.12 0.15 0.19 0.24 0.23 0 0.05 0.06 0.05 0 0 0 0 0 0 0.07 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0.07 0.14 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 0 0 1 0 2 3 4 4 3 0 0 0 0 0 0 0 2 1 0 0 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 5 1 8 13 9 21 11 19 0 0 4 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 2 7 4 7 10 12 13 15 6 11 16 51 54 15 4 6 1 0 1 1 0 1
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 0 1 0 2 3 3 0 0 0 0 1 1
Endosome 0 2 0 0 1 0 0 0 0 0 0 0 4 2 1 0 0 3 1 0 2 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 0
Mitochondria 2 6 30 19 22 65 87 137 188 145 184 0 1 6 6 1 4 3 1 2 1 1 8
Nucleus 251 326 401 304 390 353 490 490 427 348 363 202 276 280 116 76 82 259 333 406 190 230 186
Nuclear Periphery 2 2 0 1 4 5 8 5 12 6 11 0 0 2 1 0 0 0 0 0 0 0 1
Nucleolus 3 2 2 8 20 43 69 88 106 104 111 9 19 23 7 1 0 0 0 1 4 20 35
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 0 2 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 2 1 2 0 1 1 4 8 1 1 0 3 2 0 0 1 2
Vac/Vac Membrane 8 7 3 1 5 2 2 3 0 2 3 1 3 5 8 4 3 2 11 13 14 42 79
Unique Cell Count 265 341 421 318 419 406 562 597 548 435 480 220 346 361 147 86 95 275 357 431 220 307 326
Labelled Cell Count 272 347 444 343 451 487 685 749 775 626 706 229 361 388 158 90 98 275 357 431 220 307 326


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.2 8.5 6.6 6.7 6.9 6.1 5.7 5.6 5.0 4.9 4.6 9.7 9.0 7.7 12.1 13.4 14.0 10.0 10.1 9.9
Std Deviation (1e-4) 3.2 1.6 1.4 1.3 1.3 1.4 1.2 1.3 1.3 1.2 1.4 3.1 2.7 2.3 3.7 3.7 4.0 2.7 2.0 2.3
Intensity Change (Log2) 0.03 0.06 -0.11 -0.2 -0.25 -0.4 -0.43 -0.53 0.55 0.44 0.22 0.88 1.02 1.09 0.6 0.61 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 3.1 0 4.1 3.3 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.8 0.5 0.6 1.1 -0.3 0.7 3.6 6.8 6.9 4.6 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.6 -1.1 4.0 4.0 6.7 10.0 9.5 0 -4.1 -4.8 -3.6 -1.3 -2.1 -1.0
Nucleus 0.2 -1.3 -4.2 -4.3 -6.3 -7.6 -6.7 -8.2 -1.7 -6.6 -7.4 -6.0 -2.5 -3.2
Nuclear Periphery 0 0 0 0 0 3.1 0 0 0 0 0 0 0 0
Nucleolus 0 3.9 6.4 7.1 7.9 9.3 10.4 0 0 4.2 4.7 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.2352 8.847 7.9187 7.0997 7.6862 8.0104 6.0714 6.6095 6.4032 5.3749 5.8367 6.0888 10.8881 10.521 9.5963 9.504 9.4939 9.9044
Actin 0.0265 0.0009 0.0158 0.0281 0.0002 0.0003 0.0049 0 0.0092 0.0002 0.001 0.0016 0.0001 0 0 0 0 0
Bud 0.0006 0.0004 0.0006 0.0001 0.0001 0.0001 0.0003 0 0.0002 0.0001 0.0002 0.0007 0.0001 0.0002 0.0002 0 0.0001 0
Bud Neck 0.0015 0.0001 0.0005 0.0001 0.0005 0.0005 0.0006 0.0001 0.0007 0.0003 0.0002 0.0004 0.0002 0.0001 0.0001 0 0.0002 0.0003
Bud Periphery 0.0013 0.0005 0.0019 0.0001 0.0002 0.0002 0.0006 0 0.0003 0.0004 0.0005 0.001 0.0004 0.0001 0.0004 0 0.0001 0
Bud Site 0.0039 0.0037 0.0057 0.0009 0.0001 0.0001 0.0006 0 0.0014 0.0002 0.0002 0.0011 0.0001 0.0001 0.0001 0 0 0
Cell Periphery 0.0003 0.0002 0.0015 0.0001 0.0001 0.0001 0.0003 0 0.0001 0.0003 0.0003 0.0001 0.0002 0 0.0001 0 0 0
Cytoplasm 0.0036 0.0035 0.0074 0.0105 0 0 0.0065 0 0.0002 0.0002 0.0019 0.0004 0.0001 0.0179 0.0003 0 0.0001 0
Cytoplasmic Foci 0.0065 0.0012 0.0022 0.0091 0.0019 0.0001 0.0034 0 0.0008 0.0001 0.0005 0.0002 0 0.0001 0 0 0 0
Eisosomes 0.0002 0 0.0002 0.0001 0 0 0.0001 0 0.0001 0.0001 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0064 0.0016 0.0011 0.0113 0.0001 0 0.0044 0 0.0003 0.0002 0.0005 0.0007 0.0001 0.0001 0.0002 0 0 0
Endosome 0.0215 0.0023 0.0036 0.0027 0.0019 0.0002 0.0141 0 0.0021 0.0003 0.0019 0.0037 0.0002 0.0002 0.0001 0 0.0001 0
Golgi 0.0055 0.0009 0.0027 0.003 0.0017 0.0002 0.0027 0 0.0012 0.0001 0.0002 0.0011 0 0 0 0 0 0
Lipid Particles 0.0139 0.0015 0.005 0.005 0.037 0.0002 0.0054 0 0.0011 0.0012 0.0003 0.0008 0.0001 0 0 0 0 0
Mitochondria 0.0148 0.0062 0.0201 0.0011 0.003 0.0066 0.0106 0.0002 0.0016 0.0108 0.0065 0.002 0.003 0.0001 0.0006 0 0.0004 0.0001
None 0.0021 0.0004 0.0013 0.0036 0.0001 0 0.0013 0 0.0001 0.0001 0.0006 0.0001 0.0001 0 0 0 0 0
Nuclear Periphery 0.0301 0.0042 0.007 0.0071 0.0018 0.0004 0.0262 0.0035 0.0024 0.0037 0.0138 0.0056 0.0016 0.0007 0.0015 0.0006 0.0021 0.0005
Nucleolus 0.0091 0.0087 0.0064 0.0061 0.0715 0.0387 0.0195 0.0087 0.0056 0.0176 0.0442 0.0314 0.0144 0.0065 0.0065 0.0076 0.0262 0.0261
Nucleus 0.8355 0.9599 0.9039 0.8894 0.8757 0.9495 0.8783 0.9868 0.9709 0.9598 0.9114 0.9464 0.9763 0.972 0.9885 0.9916 0.9693 0.9726
Peroxisomes 0.002 0.0005 0.0016 0.0164 0.0016 0.0002 0.0014 0 0.0003 0.0001 0.0001 0.0001 0 0 0 0 0 0
Punctate Nuclear 0.0089 0.0009 0.0087 0.0045 0.0009 0.0018 0.0122 0.0004 0.0008 0.0002 0.01 0.0004 0.0002 0 0.0001 0.0002 0.0003 0.0003
Vacuole 0.003 0.002 0.0017 0.0004 0.0005 0.0004 0.0031 0.0001 0.0003 0.0013 0.0017 0.0014 0.0014 0.0016 0.0009 0 0.0005 0.0001
Vacuole Periphery 0.0029 0.0005 0.0012 0.0003 0.0009 0.0002 0.0036 0.0001 0.0004 0.0029 0.0041 0.0009 0.0011 0.0003 0.0004 0 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 56.7956 60.7302 50.1524 48.614 45.2246 41.6149 52.9034 50.3564 48.4423 53.7691
Translational Efficiency 0.904 0.744 0.8042 0.7435 0.7985 0.7987 0.6781 0.601 0.5842 0.6475

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
142 168 658 468 1169 1253 226 19 1311 1421 884 487

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 826.65 1093.55 1220.61 1251.49 871.91 1021.75 1355.31 1570.74 867.01 1030.24 1255.05 1263.95
Standard Deviation 127.49 156.97 169.68 244.76 153.80 161.17 172.95 273.01 151.82 162.35 180.36 253.58
Intensity Change Log 2 0.403671 0.562254 0.598298 0.228791 0.636372 0.849193 0.316550 0.600776 0.732523

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000392 0.000285 0.000515 0.001123 0.000351 0.000495 0.000563 0.000639 0.000356 0.000470 0.000528 0.001104
Bud Neck 0.002569 0.006709 0.010222 0.017652 0.003893 0.005194 0.007333 0.009145 0.003749 0.005373 0.009483 0.017320
Bud Site 0.000611 0.000595 0.000610 0.003210 0.000366 0.000993 0.000414 0.000482 0.000392 0.000946 0.000560 0.003104
Cell Periphery 0.000073 0.000048 0.000040 0.000217 0.000200 0.000220 0.000020 0.000029 0.000186 0.000200 0.000035 0.000209
Cytoplasm 0.000418 0.000079 0.000107 0.004399 0.000536 0.000514 0.000059 0.000344 0.000524 0.000463 0.000094 0.004241
Cytoplasmic Foci 0.000135 0.000076 0.000009 0.006380 0.000055 0.000860 0.000013 0.000015 0.000064 0.000768 0.000010 0.006131
Eisosomes 0.000027 0.000029 0.000045 0.000051 0.000046 0.000053 0.000046 0.000042 0.000044 0.000050 0.000045 0.000051
Endoplasmic Reticulum 0.004034 0.002220 0.002036 0.002064 0.005503 0.004968 0.001494 0.000973 0.005344 0.004643 0.001898 0.002021
Endosome 0.000076 0.000166 0.000033 0.006320 0.000116 0.000480 0.000019 0.000018 0.000112 0.000443 0.000029 0.006074
Golgi 0.000045 0.000035 0.000027 0.005129 0.000039 0.000684 0.000026 0.000030 0.000039 0.000608 0.000026 0.004930
Lipid Particles 0.000133 0.000038 0.000016 0.001154 0.000066 0.000963 0.000014 0.000008 0.000073 0.000854 0.000015 0.001109
Mitochondria 0.002709 0.003139 0.000859 0.004959 0.000542 0.001751 0.000942 0.001898 0.000777 0.001915 0.000880 0.004839
Mitotic Spindle 0.000657 0.002855 0.000012 0.012978 0.000119 0.005382 0.000011 0.000007 0.000177 0.005083 0.000012 0.012471
None 0.002301 0.000613 0.000556 0.004435 0.003540 0.001468 0.000497 0.000684 0.003406 0.001367 0.000541 0.004288
Nuclear Periphery 0.000173 0.000059 0.000087 0.001063 0.000143 0.000224 0.000066 0.000142 0.000146 0.000204 0.000082 0.001027
Nuclear Periphery Foci 0.000293 0.000013 0.000007 0.001672 0.000171 0.000428 0.000002 0.000002 0.000184 0.000379 0.000006 0.001607
Nucleolus 0.011788 0.005484 0.004381 0.012269 0.009905 0.007314 0.004750 0.002944 0.010109 0.007098 0.004475 0.011905
Nucleus 0.972317* 0.976861* 0.979741* 0.905158* 0.973571* 0.965445* 0.982771* 0.981850* 0.973435* 0.966795* 0.980515* 0.908151*
Peroxisomes 0.000399 0.000218 0.000228 0.001043 0.000255 0.000911 0.000477 0.000306 0.000271 0.000829 0.000292 0.001014
Vacuole 0.000451 0.000332 0.000415 0.005980 0.000463 0.001024 0.000430 0.000372 0.000462 0.000942 0.000419 0.005761
Vacuole Periphery 0.000397 0.000147 0.000056 0.002746 0.000123 0.000629 0.000052 0.000071 0.000153 0.000572 0.000055 0.002642

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.93 -1.24 -4.77 -6.26 -4.80 -2.61 -5.82 -2.99 -1.53 -0.98 -2.29 -5.89 -5.94 -5.09 -4.79
Bud Neck -1.06 -6.59 -6.31 -2.89 -3.45 -2.24 -4.82 -1.98 -1.65 -0.97 -2.39 -11.07 -6.51 -5.64 -3.78
Bud Site 0.03 -0.08 -3.87 -3.94 -4.45 -2.56 -1.67 -0.98 1.00 -0.54 -2.48 -3.12 -4.84 -3.65 -4.51
Cell Periphery 1.01 1.67 -2.46 -3.05 -3.40 -0.26 5.46 4.96 2.59 -0.73 -0.20 5.15 -0.10 0.11 -3.50
Cytoplasm 1.45 1.33 -2.20 -2.41 -2.39 -0.09 4.05 1.06 0.94 -1.42 0.12 4.05 -2.16 -2.16 -2.40
Cytoplasmic Foci 0.54 1.40 -2.37 -2.40 -2.44 -2.53 2.00 1.94 2.68 -0.14 -2.50 2.53 -2.41 -1.94 -2.44
Eisosomes -0.26 -2.81 -4.08 -5.50 -1.82 -0.77 -0.13 0.14 0.85 0.29 -0.76 -0.27 -1.20 -0.56 -1.89
Endoplasmic Reticulum 3.41 3.66 3.18 -0.06 -0.43 1.36 12.98 12.22 11.11 1.69 1.97 11.48 8.55 6.90 -0.88
Endosome -0.85 1.82 -2.68 -2.62 -2.71 -3.44 6.10 5.92 4.35 0.14 -3.51 5.59 -2.65 -2.42 -2.71
Golgi 0.36 0.75 -2.12 -2.13 -2.13 -2.86 0.79 0.97 2.93 0.41 -2.85 1.11 -2.12 -1.72 -2.13
Lipid Particles 1.12 1.41 -1.46 -1.70 -1.74 -1.35 1.80 1.95 1.44 1.64 -1.33 2.08 -1.61 0.06 -1.74
Mitochondria -0.12 1.08 -0.85 -0.43 -3.61 -2.19 -4.18 -4.40 -0.43 -3.19 -1.86 -0.80 -3.78 -2.18 -3.66
Mitotic Spindle -0.75 0.99 -4.00 -2.96 -4.19 -3.43 0.74 1.21 3.52 1.13 -3.51 1.40 -4.14 -2.75 -4.19
None 2.16 2.26 -1.74 -4.66 -4.79 2.68 4.38 4.18 1.98 -0.33 2.95 4.77 -0.80 -3.37 -4.78
Nuclear Periphery 0.93 0.57 -2.61 -3.15 -3.01 -1.59 3.22 0.00 1.42 -1.87 -1.27 2.03 -2.87 -2.64 -3.04
Nuclear Periphery Foci 1.32 1.34 -2.04 -2.64 -2.65 -1.22 2.03 2.04 2.56 1.14 -1.03 2.28 -2.26 -1.91 -2.65
Nucleolus 1.84 2.30 -0.81 -3.59 -4.52 2.12 4.44 5.88 3.76 2.02 2.62 5.72 -1.86 -3.22 -4.46
Nucleus -0.46 -0.92 5.69 5.91 7.71 2.21 -3.82 -0.88 -2.14 0.63 1.93 -3.39 7.05 6.07 7.77
Peroxisomes 1.15 1.12 -2.45 -4.27 -4.47 -2.12 -2.77 -0.24 2.01 1.89 -2.04 -0.23 -4.01 -0.20 -4.07
Vacuole 0.43 -0.02 -2.59 -2.66 -2.60 -2.58 0.36 0.62 2.74 0.41 -2.48 0.10 -2.60 -2.35 -2.60
Vacuole Periphery 0.95 1.31 -1.72 -2.11 -2.22 -2.36 5.21 2.78 2.59 -1.42 -2.19 3.14 -2.10 -1.51 -2.22