Standard name
Human Ortholog
Description Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.06 0.05 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.09 0 0 0 0 0.09 0.05 0.05 0.05 0.06 0.06 0.1 0.17 0.2 0.07 0.1 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.05 0.05 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.08 0 0.09 0 0.16 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.79 0.74 0.72 0.67 0.81 0.78 0.64 0.72 0.66 0.65 0.61 0.62 0.82 0.67 0.65 0.47 0.53 0.64 0.38 0.57 0.47 0.33 0.25 0.21
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.26 0.06 0.06 0.1 0.12 0.13 0.13 0.11 0.13 0 0.06 0.05 0.1 0.14 0.1 0 0 0 0.07 0.12 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.24 0.23 0.08 0.13 0.12 0.14 0.13 0.11 0.13 0.15 0.16 0.09 0.13 0.12 0.27 0.21 0.15 0.43 0.29 0.34 0.39 0.4 0.44
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 0 0 2 1 2 1 1 6 1 3 0 1 0 0 1 0 0 0 0 0 0 1
Bud 1 0 0 0 0 1 1 1 2 6 1 4 0 0 0 0 0 0 14 4 10 12 20 23
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 1 4 2 11 17
Bud Site 0 0 0 0 0 2 2 4 4 7 2 6 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1 1 13 5 0 3 1 0 0 0 0 0 0
Cytoplasm 9 5 6 4 10 10 25 18 17 16 13 17 19 69 79 10 18 9 7 2 9 5 13 9
Endoplasmic Reticulum 3 1 0 0 0 1 0 3 1 1 0 0 0 0 2 5 4 3 20 1 6 7 11 15
Endosome 1 0 0 2 2 2 2 4 3 4 1 10 0 1 3 0 0 2 1 0 1 2 11 11
Golgi 2 1 2 1 5 3 1 1 0 1 0 1 2 7 7 28 10 5 0 0 0 0 1 2
Mitochondria 1 0 0 2 0 1 22 3 29 12 33 10 0 0 0 1 3 5 0 0 0 1 1 1
Nucleus 193 40 107 87 275 176 174 245 207 198 125 179 160 272 256 66 96 71 131 60 105 68 107 89
Nuclear Periphery 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 4 0 2 3 6 10
Nucleolus 2 1 4 34 19 13 27 42 42 41 22 39 3 24 19 14 26 11 6 0 4 14 51 37
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 0 0 1 0 0 0 1 0 0 1 0 1 0 0 4 16 9
Vac/Vac Membrane 39 13 34 11 45 27 38 43 35 41 31 45 18 52 47 37 38 17 150 30 76 82 173 187
Unique Cell Count 245 54 148 130 340 225 274 342 312 305 206 289 194 409 396 139 182 111 349 106 224 208 431 424
Labelled Cell Count 253 61 153 141 358 238 295 366 342 334 229 315 203 440 418 161 200 124 349 106 224 208 431 424


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.0 22.0 16.5 15.6 16.3 14.3 12.7 13.9 12.0 12.6 11.6 12.7 17.2 18.1 18.3 25.3 25.5 24.7 18.5 18.5 19.7
Std Deviation (1e-4) 2.3 5.3 2.4 2.8 2.7 2.4 2.6 2.4 2.2 2.5 2.3 2.3 3.5 4.2 3.9 6.3 5.5 6.1 5.7 3.3 3.7
Intensity Change (Log2) -0.08 -0.02 -0.2 -0.38 -0.24 -0.45 -0.38 -0.51 -0.37 0.07 0.14 0.15 0.62 0.63 0.58 0.17 0.17 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.2 1.9 0.6 0.6 0.6 0.9 0.8 2.0 3.9 4.5 1.2 2.0 1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 5.2 2.0 0
Mitochondria 0 0 0 3.5 0 3.8 0 5.1 0 0 0 0 0 0 0
Nucleus -1.0 2.1 1.3 -1.8 -0.1 -1.3 -1.6 -2.3 -2.2 2.3 -1.3 -1.7 -4.3 -3.6 -1.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 5.7 1.4 1.4 2.7 3.3 3.6 3.6 2.8 3.6 0 1.5 1.1 2.6 3.6 2.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.3 -2.7 -2.8 -2.4 -2.9 -3.3 -2.6 -1.9 -1.9 -3.5 -3.0 -3.2 0.7 -0.5 -1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.1308 14.1011 12.5163 13.3442 11.6351 12.7616 10.5392 13.2864 12.7284 11.6719 11.6534 11.7209 15.5283 16.5867 15.4959 16.0628 15.341 14.8957
Actin 0.0088 0 0 0 0.0037 0 0.0479 0 0.0119 0.0001 0 0.0049 0.0064 0.0027 0.0001 0 0 0.0002
Bud 0.0002 0.0001 0 0 0.0001 0.0001 0.0007 0.0001 0.0003 0.0002 0.0001 0.0005 0 0.0001 0.0002 0.0001 0 0.0003
Bud Neck 0.0009 0.0006 0.0008 0.0007 0.002 0.0097 0.0039 0.0009 0.0036 0.0038 0.0011 0.0214 0.0003 0.0008 0.0007 0.0008 0.0019 0.0124
Bud Periphery 0.0005 0 0.0001 0 0.0001 0.0003 0.002 0.0001 0.0006 0.0002 0.0001 0.0012 0.0001 0.0001 0.0001 0.0001 0 0.0003
Bud Site 0.0005 0.0005 0.0003 0.0002 0.0003 0.0003 0.0024 0.0009 0.002 0.0004 0.0001 0.0008 0.0001 0.0006 0.0003 0.0004 0.0001 0.0004
Cell Periphery 0.002 0.0002 0.0006 0.0001 0.0001 0.0009 0.0018 0.0016 0.0033 0.0002 0.0001 0.0015 0.0001 0.0006 0.0004 0.0003 0.0002 0.0007
Cytoplasm 0.0033 0.0015 0.0012 0.0007 0.0008 0.0015 0.0134 0.0023 0.0016 0.0026 0.0007 0.0018 0.0006 0.0046 0.001 0.0002 0.0009 0.002
Cytoplasmic Foci 0.0017 0 0.0001 0 0.0013 0.0001 0.0286 0 0.0003 0 0 0.0003 0.0002 0.0003 0.0001 0 0 0.0001
Eisosomes 0.0001 0 0 0 0.0001 0 0.0005 0 0.0001 0 0 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0017 0 0.0002 0.0001 0.0005 0.0004 0.0097 0.0002 0.0011 0.0004 0 0.0007 0.0001 0.0002 0.0001 0 0.0001 0.0002
Endosome 0.0034 0 0.0005 0.0001 0.0064 0.0005 0.0333 0.0001 0.0022 0.0001 0 0.0025 0.0001 0.0005 0 0 0.0001 0.0002
Golgi 0.0011 0 0 0 0.0028 0 0.0048 0 0.0008 0 0 0.0008 0.0003 0.0004 0 0 0 0
Lipid Particles 0.0014 0 0.0001 0 0.0067 0.0005 0.0093 0.0001 0.0006 0 0 0.0012 0 0.0006 0 0 0.0001 0.0001
Mitochondria 0.0021 0.0001 0.0002 0 0.0011 0.0001 0.0031 0.0001 0.0007 0.0001 0.0001 0.0049 0.0001 0.0002 0.0002 0 0 0.0001
None 0.0007 0 0.0004 0 0.0005 0 0.0094 0 0.0006 0 0 0.0001 0.0001 0.0001 0 0 0 0
Nuclear Periphery 0.0081 0.0019 0.0024 0.0015 0.0054 0.0043 0.0166 0.0039 0.0061 0.0058 0.0015 0.0043 0.0019 0.0032 0.0022 0.0011 0.0023 0.0028
Nucleolus 0.0265 0.0429 0.0458 0.0309 0.0363 0.0522 0.023 0.0366 0.0661 0.0177 0.0221 0.0378 0.0169 0.0387 0.0548 0.0704 0.0453 0.0446
Nucleus 0.9254 0.9509 0.9436 0.9619 0.922 0.9205 0.7607 0.9464 0.8838 0.965 0.9733 0.9048 0.9658 0.9317 0.9348 0.9255 0.9086 0.9283
Peroxisomes 0.0005 0 0 0 0.0015 0 0.0046 0 0.0002 0 0 0.0002 0 0.0004 0 0 0 0
Punctate Nuclear 0.0046 0.0005 0.0008 0.0031 0.007 0.005 0.0152 0.0006 0.0016 0.0011 0.0003 0.0012 0.0061 0.0107 0.0038 0.0003 0.0397 0.005
Vacuole 0.006 0.0008 0.0026 0.0006 0.0012 0.0033 0.0075 0.0058 0.0118 0.0022 0.0004 0.0082 0.0005 0.0034 0.0011 0.0007 0.0004 0.0022
Vacuole Periphery 0.0006 0 0.0003 0 0.0003 0.0001 0.0016 0.0001 0.0007 0.0001 0 0.0007 0.0001 0.0001 0.0001 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 63.8244 53.187 47.3604 53.2101 54.6999 28.1104 46.6884 52.7942 46.8489 59.6977
Translational Efficiency 1.2819 1.577 1.4636 1.2222 1.2913 2.4266 1.5281 1.1495 1.2945 1.098

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1645 922 3018 1429 2436 1402 1881 1239 4081 2324 4899 2668

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1431.17 1620.62 1876.36 2090.57 1462.44 1551.29 1991.81 1846.56 1449.84 1578.80 1920.69 1977.25
Standard Deviation 235.09 269.87 294.71 433.35 235.74 238.73 318.59 667.51 235.98 253.82 309.24 567.72
Intensity Change Log 2 0.179351 0.390742 0.546701 0.085091 0.445703 0.336463 0.132481 0.418781 0.444274

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001390 0.002230 0.001043 0.002514 0.001038 0.001465 0.001937 0.002273 0.001180 0.001769 0.001386 0.002402
Bud Neck 0.098933 0.168736 0.222765* 0.324537* 0.131885 0.126596 0.313515* 0.244111* 0.118602 0.143314 0.257609* 0.287188*
Bud Site 0.023719 0.027697 0.028441 0.046228 0.023216 0.025925 0.043958 0.065299 0.023419 0.026628 0.034398 0.055084
Cell Periphery 0.004458 0.002759 0.005413 0.002985 0.005521 0.002087 0.002363 0.001897 0.005093 0.002354 0.004242 0.002479
Cytoplasm 0.017103 0.017089 0.008573 0.011532 0.006707 0.008999 0.004519 0.127333* 0.010897 0.012208 0.007017 0.065309
Cytoplasmic Foci 0.000869 0.001031 0.000227 0.003071 0.000463 0.002360 0.000553 0.019461 0.000627 0.001833 0.000352 0.010682
Eisosomes 0.000254 0.000236 0.000321 0.000356 0.000334 0.000233 0.000406 0.000232 0.000302 0.000234 0.000354 0.000298
Endoplasmic Reticulum 0.023863 0.016023 0.020940 0.013103 0.030872 0.020894 0.011251 0.006753 0.028046 0.018961 0.017220 0.010154
Endosome 0.000784 0.000857 0.001062 0.002526 0.000978 0.001382 0.000477 0.007446 0.000900 0.001174 0.000837 0.004811
Golgi 0.000135 0.000145 0.000066 0.002618 0.000082 0.000844 0.000074 0.006918 0.000103 0.000567 0.000069 0.004615
Lipid Particles 0.000280 0.000391 0.000206 0.001165 0.000193 0.000473 0.000176 0.002587 0.000228 0.000440 0.000194 0.001825
Mitochondria 0.001647 0.002693 0.002817 0.005653 0.001655 0.002193 0.004043 0.006542 0.001652 0.002391 0.003288 0.006066
Mitotic Spindle 0.000636 0.003116 0.002240 0.010494 0.000593 0.008209 0.000274 0.043587 0.000610 0.006189 0.001485 0.025862
None 0.002080 0.003706 0.001587 0.003500 0.001475 0.001378 0.001897 0.007005 0.001719 0.002301 0.001706 0.005128
Nuclear Periphery 0.000974 0.000974 0.002101 0.001996 0.001336 0.001225 0.001099 0.002033 0.001190 0.001125 0.001717 0.002013
Nuclear Periphery Foci 0.000978 0.000767 0.000270 0.000831 0.000597 0.001274 0.000154 0.003950 0.000751 0.001073 0.000226 0.002280
Nucleolus 0.004093 0.006889 0.002113 0.012685 0.007659 0.004685 0.007087 0.015510 0.006221 0.005559 0.004023 0.013997
Nucleus 0.809433* 0.735742* 0.690852* 0.540176* 0.777684* 0.780006* 0.599558* 0.381402* 0.790482* 0.762445* 0.655799* 0.466442*
Peroxisomes 0.000132 0.000282 0.000044 0.000257 0.000108 0.000290 0.000182 0.000943 0.000118 0.000287 0.000097 0.000575
Vacuole 0.008010 0.008451 0.008789 0.012540 0.007426 0.008904 0.006326 0.050768 0.007661 0.008724 0.007843 0.030293
Vacuole Periphery 0.000228 0.000186 0.000130 0.001233 0.000178 0.000579 0.000149 0.003950 0.000198 0.000423 0.000138 0.002495

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.04 -0.49 -10.60 -2.36 -11.37 -6.12 -18.32 -7.41 -3.87 0.77 -6.60 -11.59 -13.33 -5.30 -7.38
Bud Neck -9.59 -21.81 -29.35 -16.39 -12.81 0.48 -25.94 -16.59 -15.80 4.67 -5.89 -31.58 -31.97 -24.15 -6.80
Bud Site -1.98 -4.12 -11.17 -8.38 -8.26 -1.45 -9.58 -11.60 -9.63 -4.52 -2.33 -9.13 -16.23 -12.84 -9.35
Cell Periphery 2.87 -0.23 3.22 0.01 5.08 6.39 6.56 7.19 1.47 1.92 6.82 3.44 7.31 0.31 5.84
Cytoplasm 0.52 5.60 4.19 2.83 -1.38 -2.02 3.76 -14.76 -14.28 -15.31 -0.71 5.15 -12.85 -12.32 -14.53
Cytoplasmic Foci -1.27 1.79 -5.82 -5.22 -6.50 -2.40 -4.11 -8.90 -6.37 -8.47 -2.57 -0.65 -10.55 -7.05 -10.37
Eisosomes 0.57 -3.22 -1.93 -2.18 -0.66 5.03 -2.13 1.13 -0.70 2.11 4.27 -2.86 -0.40 -2.13 0.84
Endoplasmic Reticulum 6.42 4.07 11.28 5.51 9.57 6.90 18.55 20.12 13.83 5.53 9.18 14.34 22.45 14.03 11.65
Endosome -0.54 -0.70 -3.08 -2.82 -2.13 -1.91 7.27 -5.27 -4.46 -6.23 -1.99 1.18 -5.99 -4.95 -6.09
Golgi -0.11 1.22 -2.36 -2.35 -2.47 -2.01 -2.49 -2.93 -2.15 -2.90 -2.01 0.63 -3.72 -2.78 -3.74
Lipid Particles -1.73 -0.67 -2.74 -2.25 -2.65 -2.82 -2.96 -3.52 -2.68 -3.32 -3.25 -2.88 -4.51 -3.39 -4.22
Mitochondria -7.54 -15.18 -17.90 -11.49 -9.23 -2.94 -20.60 -5.93 -4.98 -0.89 -6.37 -24.39 -12.66 -9.71 -5.00
Mitotic Spindle -1.32 -2.58 -4.15 -2.42 -2.94 -3.56 -0.76 -8.46 -5.81 -8.24 -3.74 -2.70 -9.32 -5.71 -8.39
None -1.07 1.20 -2.56 0.20 -3.72 0.42 -0.91 -7.86 -8.29 -7.18 -0.89 -0.23 -7.76 -3.63 -7.99
Nuclear Periphery -0.64 -10.97 -7.90 -7.32 0.01 0.81 0.42 -2.51 -2.86 -2.75 0.40 -8.41 -6.59 -6.61 -1.40
Nuclear Periphery Foci 1.10 3.99 0.48 -0.82 -4.48 -1.55 3.56 -2.87 -1.76 -3.89 -0.70 5.04 -2.57 -1.70 -5.06
Nucleolus -2.18 1.93 -7.90 -4.99 -9.03 3.14 -2.48 -7.53 -8.90 -6.05 0.90 0.83 -10.25 -10.37 -10.73
Nucleus 7.91 17.28 28.88 17.60 15.67 0.02 21.93 34.26 30.94 13.72 4.95 26.05 43.57 34.38 21.68
Peroxisomes -2.38 2.98 -5.46 -1.87 -7.01 -2.79 -4.60 -5.98 -4.47 -4.53 -3.62 -2.05 -7.58 -4.74 -6.91
Vacuole -0.76 -4.21 -6.98 -6.12 -4.31 -2.64 -1.27 -10.09 -9.36 -9.80 -2.65 -4.77 -11.62 -10.52 -10.39
Vacuole Periphery 0.47 1.15 -2.61 -2.97 -3.24 -1.96 -0.22 -2.59 -2.00 -2.58 -1.74 1.29 -3.37 -2.66 -3.52