Standard name | |
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Description | Cytochrome c1; component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 3 | 0 | 1 | 0 | 1 | 0 | 3 | – | 1 | 0 | 0 | 2 | 1 | 3 | 2 | 3 | 5 | 6 | 29 | 6 | 8 | 7 | 14 |
Bud | 0 | 5 | 8 | 2 | 6 | 14 | 14 | 13 | – | 5 | 10 | 17 | 1 | 2 | 2 | 0 | 4 | 2 | 0 | 1 | 0 | 0 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 6 | 5 | 2 | – | 6 | 6 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 15 | 3 | 2 | 3 | 0 | 1 | 1 | – | 1 | 1 | 0 | 3 | 3 | 7 | 5 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 3 | 4 | 1 | 2 | 6 | 6 | 4 | – | 3 | 2 | 6 | 3 | 3 | 1 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 1 | 11 | 5 | 4 | – | 4 | 6 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1 | 1 | 6 | 3 | 7 | 0 | 3 | 4 | – | 2 | 0 | 1 | 6 | 14 | 19 | 0 | 1 | 1 | 0 | 12 | 10 | 5 | 6 | 5 |
Golgi | 3 | 15 | 0 | 2 | 1 | 2 | 0 | 0 | – | 0 | 2 | 1 | 29 | 37 | 51 | 8 | 19 | 11 | 5 | 38 | 16 | 14 | 25 | 26 |
Mitochondria | 47 | 316 | 250 | 151 | 219 | 318 | 426 | 525 | – | 410 | 334 | 341 | 107 | 106 | 136 | 0 | 4 | 6 | 48 | 253 | 192 | 86 | 99 | 106 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 4 | – | 4 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 10 | 11 | 3 | 10 | 24 | 44 | 46 | – | 37 | 28 | 31 | 2 | 3 | 3 | 1 | 3 | 3 | 1 | 1 | 0 | 1 | 2 | 2 |
Nucleolus | 8 | 5 | 14 | 5 | 18 | 44 | 65 | 88 | – | 54 | 42 | 52 | 5 | 2 | 5 | 0 | 3 | 1 | 1 | 0 | 0 | 1 | 0 | 1 |
Peroxisomes | 0 | 20 | 55 | 7 | 8 | 4 | 19 | 15 | – | 12 | 5 | 5 | 15 | 6 | 18 | 2 | 13 | 3 | 1 | 20 | 53 | 21 | 27 | 22 |
SpindlePole | 2 | 1 | 4 | 2 | 0 | 1 | 8 | 6 | – | 9 | 3 | 1 | 2 | 1 | 7 | 0 | 3 | 0 | 2 | 0 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 5 | 30 | 4 | 4 | 22 | 7 | 7 | 14 | – | 10 | 5 | 6 | 14 | 17 | 38 | 2 | 8 | 9 | 0 | 3 | 0 | 1 | 1 | 7 |
Unique Cell Count | 64 | 343 | 273 | 158 | 243 | 366 | 483 | 605 | 453 | 361 | 373 | 137 | 143 | 208 | 17 | 58 | 38 | 74 | 370 | 285 | 144 | 175 | 195 | |
Labelled Cell Count | 74 | 426 | 359 | 183 | 297 | 441 | 606 | 729 | 558 | 444 | 465 | 190 | 198 | 292 | 21 | 67 | 42 | 74 | 370 | 285 | 144 | 175 | 195 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 18.4 | 14.4 | 9.6 | 9.8 | 9.3 | 9.3 | 9.1 | 10.0 | – | 10.3 | 11.2 | 11.6 | 10.6 | 10.6 | 10.2 | 14.7 | 14.0 | 14.6 | 12.3 | 12.9 | 13.0 |
Std Deviation (1e-4) | 8.6 | 3.9 | 2.9 | 4.6 | 3.3 | 3.6 | 3.5 | 3.8 | – | 4.3 | 4.7 | 4.5 | 2.7 | 2.8 | 4.6 | 3.7 | 3.8 | 3.9 | 3.6 | 4.4 | 3.9 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.04 | -0.04 | -0.08 | 0.07 | – | 0.1 | 0.22 | 0.27 | 0.14 | 0.14 | 0.1 | 0.62 | 0.55 | 0.61 | 0.36 | 0.43 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | -0.3 | 0.6 | 0 | -0.7 | – | 0 | -0.1 | 1.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0.5 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3.4 | 3.4 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 7.9 | 8.8 | 8.7 | 0 | 0 | 0 |
Mitochondria | 1.6 | -0.6 | -1.9 | -1.4 | -2.0 | – | -0.5 | 0.4 | -0.1 | -3.8 | -4.8 | -7.1 | -10.6 | -13.9 | -11.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.2 | 0 | 1.4 | 2.6 | 2.0 | – | 2.2 | 1.9 | 2.2 | 0 | 0 | -1.7 | 0 | 0 | 0 |
Nucleolus | -1.0 | 1.1 | 3.0 | 3.6 | 4.0 | – | 3.0 | 2.9 | 3.7 | -0.7 | -1.9 | -1.5 | 0 | 0 | 0 |
Peroxisomes | -4.5 | -5.8 | -8.2 | -7.2 | -8.9 | – | -7.9 | -8.0 | -8.1 | -2.3 | -4.4 | -3.5 | 0 | 0.4 | -1.8 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.9 | 0 | 0 | 0.8 | – | 0.7 | 0 | 0 | 4.1 | 4.6 | 6.5 | 0 | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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