Standard name
Human Ortholog
Description Cis-golgi GTPase-activating protein (GAP) for yeast Rabs; the Rab family members are Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.07 0 0 0.05 0 0.06 0.05 0 0.05 0 0 0 0 0 0 0.06 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 0.85 0.85 0.84 0.77 0.62 0.56 0.46 0.47 0.39 0.4 0.95 0.86 0.88 0.8 0.79 0.87 0.39 0.56 0.46 0.68 0.79 0.77
Endoplasmic Reticulum 0.05 0.07 0 0.05 0.06 0.09 0 0.07 0.08 0.07 0.09 0.05 0.05 0 0 0 0 0 0 0 0 0 0
Endosome 0.16 0 0 0.06 0.11 0.12 0.17 0.12 0.13 0 0.1 0 0.05 0.08 0.1 0.1 0.05 0.25 0.1 0.06 0.05 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0.15 0.18 0.32 0.28 0.6 0.36 0 0 0 0 0 0 0.12 0.14 0.25 0.12 0.06 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.06 0.05 0.06 0 0.06 0 0 0 0.12 0.15 0.09 0 0.07 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 9 11 5 10 12 13 11 16 9 17 1 7 2 3 5 2 12 11 6 2 5 7
Bud 3 2 0 1 0 4 13 9 9 12 16 1 1 0 0 0 0 1 1 1 0 0 4
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 3 2 4 4
Bud Site 0 0 0 0 0 0 1 0 0 2 2 0 0 0 0 0 0
Cell Periphery 2 1 2 1 3 2 3 4 12 4 8 4 2 0 7 1 3 0 0 1 0 0 0
Cytoplasm 133 217 143 144 188 139 193 90 141 111 134 58 204 220 138 108 113 87 166 96 92 277 246
Endoplasmic Reticulum 10 17 5 9 15 19 6 14 24 19 30 3 11 2 7 3 5 5 6 9 0 2 4
Endosome 31 9 7 10 26 26 58 24 38 12 32 0 13 20 18 13 6 57 30 11 7 11 12
Golgi 0 1 0 0 2 1 3 1 1 0 2 0 0 0 2 0 2 11 5 4 0 1 1
Mitochondria 11 4 0 7 3 33 61 63 84 172 118 0 0 0 3 1 2 27 40 51 16 19 22
Nucleus 0 2 1 1 1 4 1 0 0 2 4 0 1 0 2 1 0 0 0 0 2 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 8 0 3 1 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 2 4 6 4 14 6 4 3 20 0 5 3 2 0 1 5 5 2 2 3 3
Vac/Vac Membrane 8 5 6 1 7 6 20 9 18 8 21 0 4 10 21 20 12 9 19 12 4 11 5
Unique Cell Count 191 255 168 172 245 223 345 194 299 288 331 61 237 251 172 136 130 226 296 209 135 349 319
Labelled Cell Count 207 268 178 184 261 250 394 231 350 355 405 67 248 257 203 153 146 226 296 209 135 349 319


Cytoplasm, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 6.6 7.2 6.7 7.5 6.4 6.6 6.0 6.5 5.5 5.9 6.9 6.9 6.9 9.6 9.7 9.1 6.3 6.7 7.0
Std Deviation (1e-4) 0.7 0.9 1.1 1.9 1.5 1.1 1.4 1.2 1.7 1.0 1.3 0.9 0.9 1.2 2.4 2.4 2.1 0.9 1.0 1.6
Intensity Change (Log2) -0.1 0.04 -0.18 -0.13 -0.26 -0.16 -0.4 -0.3 -0.06 -0.06 -0.08 0.41 0.42 0.33 -0.2 -0.12 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.6 -1.1 -0.5 -1.4 -0.3 -0.5 -1.7 -0.6 0 -1.7 -3.3 -2.2 -1.1 -2.1
Bud 0 0 0 0 0 0 0 2.9 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1.7 0 0 0 0 0 0 0 0
Cytoplasm -0.4 -2.1 -5.0 -6.5 -7.7 -8.1 -9.7 -9.5 2.0 0.3 0.7 -1.2 -1.3 0.4
Endoplasmic Reticulum 1.0 1.5 2.3 0 1.8 2.2 1.7 2.5 0 0.8 0 0.5 0 0
Endosome 0.7 2.4 2.6 4.0 2.8 3.0 0 2.2 0 0.6 1.6 2.2 1.9 0.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.2 5.8 8.1 7.6 12.7 8.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1.8 0 0 0 2.5 0 0 0 0 0 0
Vacuole 0 -0.4 -0.5 1.1 0.5 1.2 -0.5 1.3 0 0 0.2 2.9 3.5 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.3521 0.6112 0.3857 0.1421 -0.9705 0.3404 1.7031 2.1432 1.9442 1.8845 1.8172 1.8335 0.7684 0.6781 0.2492 1.0332 0.4227 0.6462
Actin 0.0083 0.0085 0.0085 0.002 0.0024 0.0008 0.0228 0.0045 0.0412 0.0131 0.027 0.0108 0.0745 0.0012 0.0016 0.0508 0.0783 0.0144
Bud 0.0006 0.002 0.0007 0.0003 0.0004 0.0001 0.0041 0.0009 0.0045 0.001 0.0008 0.0057 0.0006 0.0004 0.0004 0.0002 0.0005 0.0002
Bud Neck 0.0052 0.0037 0.0061 0.0013 0.0002 0.0006 0.0021 0.0013 0.0032 0.0006 0.0007 0.0041 0.0007 0.0002 0.0002 0.0003 0.001 0.0009
Bud Periphery 0.0008 0.0016 0.001 0.0006 0.0005 0.0001 0.0084 0.0008 0.0048 0.0012 0.0007 0.0028 0.0006 0.0002 0.0002 0.0002 0.0009 0.0003
Bud Site 0.0084 0.0228 0.0028 0.0013 0.0015 0.0001 0.0125 0.0107 0.007 0.0021 0.0013 0.0005 0.002 0.0097 0.0006 0.0014 0.0015 0.0003
Cell Periphery 0.0013 0.0006 0.0005 0.0012 0.0003 0.0001 0.0008 0.0003 0.0003 0.0004 0.0001 0.0002 0.0002 0.0003 0.0002 0.0002 0.0003 0.0001
Cytoplasm 0.2304 0.4443 0.4136 0.4076 0.2054 0.409 0.3244 0.5635 0.4544 0.3283 0.3788 0.5107 0.3209 0.6096 0.4725 0.2472 0.254 0.5203
Cytoplasmic Foci 0.1419 0.0967 0.0707 0.1439 0.0848 0.1636 0.121 0.0818 0.1174 0.1561 0.1451 0.1191 0.072 0.0797 0.0908 0.0576 0.1469 0.1087
Eisosomes 0.0015 0.0004 0.0011 0.0005 0.0003 0 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001 0.0008 0.0001 0.0001 0.0003 0.0013 0.0002
Endoplasmic Reticulum 0.0095 0.0067 0.0076 0.0064 0.0087 0.0056 0.0141 0.0088 0.0067 0.0069 0.0088 0.009 0.0112 0.0062 0.0122 0.0176 0.0094 0.006
Endosome 0.137 0.1222 0.1594 0.069 0.1297 0.1948 0.1318 0.0906 0.1394 0.2087 0.1861 0.1356 0.1594 0.1043 0.2221 0.1591 0.2141 0.1559
Golgi 0.029 0.0115 0.0148 0.0067 0.0044 0.0162 0.0235 0.015 0.0244 0.0241 0.0444 0.0179 0.0177 0.0105 0.0132 0.0158 0.0535 0.0229
Lipid Particles 0.042 0.0033 0.0105 0.0025 0.003 0.0072 0.0208 0.0059 0.0066 0.004 0.0097 0.003 0.015 0.0014 0.0022 0.0357 0.0889 0.0081
Mitochondria 0.0119 0.0211 0.0095 0.0024 0.0025 0.003 0.0171 0.0149 0.0079 0.0075 0.0182 0.0148 0.0099 0.0041 0.0068 0.0036 0.0203 0.0057
None 0.3127 0.2343 0.2643 0.3416 0.5435 0.1812 0.2181 0.1856 0.1455 0.2053 0.1459 0.151 0.2566 0.1588 0.1596 0.3795 0.0122 0.1437
Nuclear Periphery 0.0031 0.0014 0.002 0.001 0.0011 0.0007 0.0184 0.0012 0.003 0.0038 0.002 0.0035 0.0162 0.0014 0.0024 0.0052 0.0152 0.0021
Nucleolus 0.0048 0.0002 0.0004 0.0002 0.0003 0.0001 0.0012 0.0002 0.0014 0.0007 0.0001 0.0001 0.0013 0.0001 0.0001 0.0002 0.001 0.0001
Nucleus 0.0028 0.001 0.0009 0.0013 0.0009 0.0006 0.017 0.0011 0.0092 0.0131 0.0011 0.0011 0.0063 0.0011 0.0008 0.0009 0.0022 0.0007
Peroxisomes 0.0155 0.0077 0.0108 0.0018 0.0008 0.0059 0.0263 0.002 0.0103 0.0043 0.0176 0.0036 0.0088 0.0012 0.0009 0.0097 0.0453 0.0028
Punctate Nuclear 0.0094 0.0012 0.0027 0.0014 0.0011 0.0009 0.0039 0.0007 0.0031 0.0052 0.0012 0.0009 0.0179 0.0008 0.0006 0.0028 0.0132 0.0012
Vacuole 0.021 0.0067 0.0098 0.006 0.007 0.0074 0.0088 0.0086 0.0075 0.0112 0.0082 0.0039 0.0051 0.0071 0.0095 0.009 0.0347 0.0036
Vacuole Periphery 0.0029 0.0022 0.0023 0.0008 0.0011 0.0021 0.0027 0.0015 0.0021 0.0024 0.0022 0.0016 0.0023 0.0013 0.003 0.0028 0.0058 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.2593 11.3377 7.6482 11.8605 12.7066 12.6758 12.7662 12.8365 12.3915 14.3745
Translational Efficiency 0.5576 0.8589 0.8923 0.6162 0.5806 0.9885 0.8873 0.8904 0.9307 0.6755

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency