Standard name
Description Component of the spindle checkpoint; involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability; recruits condensin to the pericentric region of chromosomes during meiosis; dissociates from pericentromeres when sister kinetochores are under tension

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0.05 0.08 0.05 0.05 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.77 0.82 0.79 0.78 0.67 0.81 0.79 0.67 0.71 0.52 0.63 0.7 0.66 0.72 0.46 0.57 0.37 0.39 0.79 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.07 0 0 0 0 0 0
Endosome 0 0.07 0 0 0 0 0 0 0 0 0 0.11 0.13 0.1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0 0 0 0 0 0.07 0.28 0.19 0.53 0.38 0 0 0 0.15 0 0.15 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.06 0.09 0.15 0.17 0.25 0.14 0.14 0.11 0.14 0.06 0.08 0.17 0.16 0.12 0.23 0.21 0.3 0.59 0 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.05 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 1 0 0 1 0 0 0 2 0 0 0 0
Bud 0 0 0 0 2 0 2 3 2 3 6 0 0 1 0 6 1 0 0 1
Bud Neck 1 2 0 6 18 9 11 6 12 5 8 2 2 2 0 2 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Cell Periphery 0 0 0 1 0 0 1 2 1 0 1 2 1 2 0 0 0 0 0 0
Cytoplasm 79 134 31 95 155 158 166 112 141 74 102 151 115 138 52 109 23 1 13 38
Endoplasmic Reticulum 2 0 0 1 2 0 0 0 0 0 1 17 11 14 0 0 1 0 0 0
Endosome 3 11 1 2 6 5 0 2 1 1 2 23 22 19 2 6 1 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0
Mitochondria 18 1 1 2 1 6 15 47 38 76 61 6 3 8 16 6 9 0 0 1
Nucleus 0 4 0 2 5 1 6 5 4 3 5 7 2 4 0 5 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 3 1 1 1 5 9 1 0 4 0 0 0 0
Peroxisomes 0 1 0 0 0 1 0 0 0 0 0 1 2 0 0 0 0 0 0 0
SpindlePole 6 14 6 21 58 27 29 18 29 9 13 36 28 22 26 39 18 2 0 2
Vac/Vac Membrane 1 3 1 0 4 2 0 2 3 4 2 9 13 12 5 3 2 0 0 0
Unique Cell Count 102 164 39 122 232 196 210 167 200 143 162 216 174 191 113 191 63 5 17 49
Labelled Cell Count 110 170 40 130 251 210 230 200 234 176 203 260 210 224 113 191 63 5 17 49


Nucleus, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.3 4.6 4.5 5.1 4.2 4.1 3.5 3.7 3.2 3.6 5.7 5.7 5.3 4.6
Std Deviation (1e-4) 0.4 1.1 0.6 1.2 1.6 1.4 1.5 1.2 1.3 1.0 1.3 1.3 1.4 1.2 1.5
Intensity Change (Log2) -0.02 0.16 -0.13 -0.15 -0.39 -0.31 -0.5 -0.34 0.33 0.32 0.22 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sgo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available