Standard name | |
---|---|
Human Ortholog | |
Description | Bifunctional triacylglycerol lipase and LPA acyltransferase; lipid particle-localized triacylglycerol (TAG) lipase involved in triacylglycerol mobilization; catalyzes acylation of lysophosphatidic acid (LPA); potential Cdc28p substrate; TGL5 has a paralog, TGL4, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 4 | 0 | 2 | 1 | 2 | 0 | 2 | – | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 3 | 8 |
Bud | 6 | 6 | 3 | 2 | 7 | 3 | 12 | 21 | 12 | – | 13 | 7 | 14 | 3 | 2 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 3 | 0 |
Bud Neck | 1 | 0 | 0 | 2 | 3 | 2 | 3 | 2 | 2 | – | 2 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 2 | 0 | 0 | 3 | 3 | 3 | 0 | 2 | 7 | – | 8 | 7 | 2 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 1 | 8 | 10 | 12 | 14 | 24 | 29 | 15 | – | 5 | 15 | 6 | 15 | 13 | 3 | 5 | 2 | 0 | 0 | 1 | 1 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 1 |
Endosome | 33 | 43 | 25 | 20 | 26 | 27 | 24 | 46 | 2 | – | 13 | 26 | 86 | 193 | 209 | 72 | 66 | 56 | 78 | 109 | 95 | 40 | 110 | 146 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 13 | 9 | 3 | 3 | 7 | 16 |
Mitochondria | 104 | 102 | 37 | 30 | 21 | 50 | 131 | 114 | 164 | – | 223 | 183 | 141 | 67 | 58 | 14 | 8 | 9 | 3 | 9 | 4 | 5 | 4 | 9 |
Nucleus | 1 | 0 | 0 | 2 | 0 | 2 | 6 | 6 | 11 | – | 9 | 11 | 0 | 3 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 7 | 4 | 3 | 2 | 1 | 2 | 4 | 5 | 3 | – | 14 | 5 | 1 | 3 | 0 | 0 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 0 |
Nucleolus | 32 | 21 | 7 | 19 | 21 | 29 | 44 | 33 | 44 | – | 53 | 52 | 47 | 46 | 76 | 23 | 34 | 28 | 1 | 1 | 0 | 1 | 0 | 0 |
Peroxisomes | 58 | 32 | 22 | 22 | 34 | 33 | 29 | 28 | 9 | – | 7 | 8 | 218 | 269 | 268 | 69 | 44 | 26 | 59 | 29 | 53 | 57 | 163 | 204 |
SpindlePole | 47 | 29 | 70 | 71 | 180 | 120 | 156 | 192 | 66 | – | 74 | 87 | 109 | 168 | 231 | 74 | 66 | 63 | 19 | 18 | 22 | 15 | 64 | 38 |
Vac/Vac Membrane | 2 | 5 | 1 | 1 | 6 | 1 | 2 | 2 | 3 | – | 4 | 9 | 3 | 1 | 3 | 1 | 7 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Unique Cell Count | 176 | 167 | 128 | 137 | 244 | 207 | 316 | 355 | 247 | 307 | 296 | 375 | 436 | 485 | 158 | 138 | 109 | 185 | 188 | 192 | 130 | 368 | 434 | |
Labelled Cell Count | 296 | 243 | 180 | 184 | 316 | 287 | 437 | 480 | 341 | 427 | 411 | 627 | 770 | 863 | 259 | 236 | 191 | 185 | 188 | 192 | 130 | 368 | 434 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 6.0 | 6.1 | 5.7 | 6.0 | 4.6 | 4.8 | 5.0 | 4.1 | – | 3.9 | 4.5 | 6.1 | 6.8 | 6.8 | 9.9 | 9.5 | 9.4 | 6.3 | 6.4 | 6.6 |
Std Deviation (1e-4) | 1.4 | 1.6 | 1.2 | 1.4 | 1.4 | 1.2 | 1.3 | 1.4 | 1.1 | – | 0.9 | 1.0 | 1.7 | 1.9 | 1.6 | 2.2 | 2.1 | 1.9 | 1.5 | 1.4 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.02 | -0.41 | -0.36 | -0.29 | -0.59 | – | -0.67 | -0.46 | 0.0 | 0.16 | 0.14 | 0.69 | 0.62 | 0.61 | 0.03 | 0.05 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | 0 | 0 | 0 | 0 | 1.6 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.1 | -2.4 | 0 | 0 | -1.8 | 0 | – | – | 0 | 0.8 | 5.1 | 4.9 | 4.6 | 4.9 | 5.2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.3 | -5.1 | 0 | 0 | 0.7 | 0 | – | – | 0 | 1.8 | -3.5 | -4.7 | -4.4 | -5.0 | -4.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | 2.3 | 1.1 | 0 | 0 | 1.3 | 0 | – | – | 0 | 2.2 | 1.7 | 3.0 | 2.5 | 4.3 | 4.4 |
Peroxisomes | -0.2 | -0.8 | 0 | 0 | -3.0 | 0 | – | – | 0 | 8.0 | 8.9 | 7.7 | 4.8 | 2.8 | 1.3 |
SpindlePole | -0.5 | 3.7 | 0 | 0 | -0.1 | 0 | – | – | 0 | -5.2 | -3.3 | -1.4 | -1.3 | -1.1 | 0.5 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.3 | -0.5 | 0.2 | 0.5 | 0.7 | -0.1 | – | – | -0.5 | 0 | -1.4 | 0 | 0 | -1.0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5138 | 3.2491 | 2.2394 | 1.6951 | 1.558 | 1.9738 | 0.9628 | 3.4165 | 2.7503 | 2.9044 | 2.726 | 2.6384 | 0.689 | 3.4079 | 2.8478 | 2.3508 | 1.7637 | 2.6186 |
Actin | 0.0078 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0067 | 0.0193 | 0.0002 | 0.002 | 0.0063 | 0.0323 | 0.0004 | 0.0172 | 0 | 0.0113 | 0.0002 | 0.0387 | 0.0001 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0.0007 | 0 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0 |
Bud Neck | 0.0014 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0052 | 0.0001 | 0.0028 | 0.0015 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0006 | 0.0001 |
Bud Periphery | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0009 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0005 | 0 |
Bud Site | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0067 | 0.0002 | 0.0008 | 0.0018 | 0.0004 | 0.0001 | 0.001 | 0.0002 | 0.0011 | 0.0001 | 0.001 | 0 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0053 | 0 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0019 | 0.0001 | 0.0004 | 0.0003 | 0.0004 | 0.0001 | 0.002 | 0.0006 | 0.0001 | 0.0001 | 0.0009 | 0 |
Cytoplasmic Foci | 0.1574 | 0.1805 | 0.1678 | 0.2676 | 0.1618 | 0.1622 | 0.2547 | 0.2523 | 0.2491 | 0.1854 | 0.1679 | 0.2063 | 0.219 | 0.1993 | 0.1765 | 0.1592 | 0.1426 | 0.1323 |
Eisosomes | 0.0005 | 0 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.0015 | 0 | 0.0001 | 0 | 0 | 0.0004 | 0.0011 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0004 | 0.0005 | 0 | 0.0001 | 0.0016 | 0 |
Endosome | 0.0134 | 0.0054 | 0.0083 | 0.0021 | 0.0095 | 0.0108 | 0.029 | 0.0163 | 0.0153 | 0.0262 | 0.0144 | 0.0101 | 0.0194 | 0.0161 | 0.0118 | 0.0564 | 0.0327 | 0.0036 |
Golgi | 0.0158 | 0.012 | 0.0128 | 0.0071 | 0.01 | 0.0088 | 0.0193 | 0.0145 | 0.0136 | 0.029 | 0.0274 | 0.0102 | 0.0144 | 0.014 | 0.0154 | 0.0312 | 0.02 | 0.0096 |
Lipid Particles | 0.7218 | 0.7632 | 0.6952 | 0.6205 | 0.5972 | 0.7657 | 0.5247 | 0.6317 | 0.5815 | 0.6365 | 0.5162 | 0.6963 | 0.6362 | 0.7069 | 0.6869 | 0.6716 | 0.6748 | 0.7966 |
Mitochondria | 0.0035 | 0.0008 | 0.008 | 0.0008 | 0.0008 | 0.0036 | 0.0063 | 0.0007 | 0.0032 | 0.0086 | 0.0112 | 0.0051 | 0.0079 | 0.0005 | 0.0011 | 0.0014 | 0.0106 | 0.0006 |
None | 0.001 | 0 | 0.0001 | 0 | 0 | 0.0014 | 0.001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0034 | 0.0006 | 0 | 0.0002 | 0.0032 | 0 |
Nuclear Periphery | 0.0048 | 0.0001 | 0.0003 | 0 | 0 | 0.0034 | 0.0021 | 0.0002 | 0.0006 | 0.0002 | 0.0006 | 0.0003 | 0.0016 | 0.0008 | 0.0003 | 0.0002 | 0.0109 | 0.0005 |
Nucleolus | 0.0035 | 0.0003 | 0.0049 | 0.0006 | 0.0119 | 0.0063 | 0.0064 | 0.0037 | 0.0049 | 0.0014 | 0.0002 | 0.001 | 0.0033 | 0.0047 | 0.0004 | 0.0006 | 0.0023 | 0.0007 |
Nucleus | 0.0036 | 0 | 0.0003 | 0 | 0.0006 | 0.0015 | 0.0013 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.001 | 0.0002 | 0 | 0 | 0.0017 | 0 |
Peroxisomes | 0.0424 | 0.0339 | 0.0897 | 0.0959 | 0.1957 | 0.0147 | 0.0797 | 0.066 | 0.1034 | 0.0956 | 0.2096 | 0.0446 | 0.0332 | 0.0371 | 0.0789 | 0.0658 | 0.0333 | 0.0421 |
Punctate Nuclear | 0.0149 | 0.0031 | 0.0082 | 0.0048 | 0.0017 | 0.0124 | 0.0366 | 0.0127 | 0.0206 | 0.0045 | 0.0168 | 0.0219 | 0.0376 | 0.0173 | 0.0153 | 0.0113 | 0.0203 | 0.0135 |
Vacuole | 0.0004 | 0.0001 | 0.0018 | 0 | 0.0086 | 0.0007 | 0.0016 | 0.0004 | 0.0004 | 0.0011 | 0.0002 | 0.0013 | 0.0009 | 0.0006 | 0.0001 | 0.0006 | 0.0016 | 0.0001 |
Vacuole Periphery | 0.0007 | 0.0002 | 0.0015 | 0.0001 | 0.0005 | 0.0006 | 0.0012 | 0.0004 | 0.0003 | 0.0005 | 0.0004 | 0.0017 | 0.0005 | 0.0003 | 0.0002 | 0.0007 | 0.0019 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.5777 | 10.5213 | 10.0844 | 13.1734 | 16.5315 | 14.7039 | 14.6274 | 13.4625 | 11.7903 | 21.7703 |
Translational Efficiency | 0.4737 | 0.8924 | 0.5776 | 0.4359 | 0.6524 | 0.7088 | 0.4952 | 0.5203 | 0.6547 | 0.5571 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
817 | 1452 | 1300 | 1693 | 1735 | 1340 | 200 | 855 | 2552 | 2792 | 1500 | 2548 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 757.65 | 875.02 | 1069.67 | 1072.22 | 765.32 | 851.65 | 1213.91 | 1058.29 | 762.86 | 863.80 | 1088.90 | 1067.55 |
Standard Deviation | 96.52 | 143.97 | 153.77 | 189.69 | 122.82 | 133.75 | 157.00 | 197.63 | 115.12 | 139.65 | 161.82 | 192.50 |
Intensity Change Log 2 | — | 0.207784 | 0.497562 | 0.500998 | — | 0.154198 | 0.665526 | 0.467600 | — | 0.181105 | 0.584410 | 0.484311 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002100 | 0.003611 | 0.005338 | 0.042045 | 0.003562 | 0.003101 | 0.008237 | 0.076106 | 0.003094 | 0.003366 | 0.005725 | 0.053474 |
Bud Neck | 0.000411 | 0.001487 | 0.032814 | 0.005069 | 0.000670 | 0.001455 | 0.007995 | 0.004351 | 0.000587 | 0.001472 | 0.029504 | 0.004828 |
Bud Site | 0.000154 | 0.001189 | 0.026778 | 0.011921 | 0.001117 | 0.001747 | 0.002462 | 0.008569 | 0.000808 | 0.001457 | 0.023536 | 0.010796 |
Cell Periphery | 0.000047 | 0.000096 | 0.000423 | 0.000167 | 0.000119 | 0.000088 | 0.000091 | 0.000150 | 0.000096 | 0.000092 | 0.000379 | 0.000161 |
Cytoplasm | 0.000369 | 0.002782 | 0.095593 | 0.005698 | 0.004652 | 0.003129 | 0.000775 | 0.004390 | 0.003281 | 0.002949 | 0.082950 | 0.005259 |
Cytoplasmic Foci | 0.058009 | 0.157961 | 0.061828 | 0.256226* | 0.123484 | 0.152711 | 0.213656* | 0.224268* | 0.102523 | 0.155441 | 0.082071 | 0.245502* |
Eisosomes | 0.000111 | 0.000135 | 0.000065 | 0.000181 | 0.000068 | 0.000072 | 0.000183 | 0.000400 | 0.000082 | 0.000105 | 0.000080 | 0.000254 |
Endoplasmic Reticulum | 0.000391 | 0.000645 | 0.013710 | 0.000667 | 0.000549 | 0.000448 | 0.000903 | 0.000910 | 0.000498 | 0.000550 | 0.012003 | 0.000748 |
Endosome | 0.031033 | 0.071892 | 0.019293 | 0.150649 | 0.059787 | 0.124463 | 0.070701 | 0.099998 | 0.050581 | 0.097123 | 0.026148 | 0.133653 |
Golgi | 0.042824 | 0.069403 | 0.001966 | 0.110450* | 0.046189 | 0.062848 | 0.061960 | 0.173144* | 0.045112 | 0.066257 | 0.009965 | 0.131487* |
Lipid Particles | 0.761909* | 0.588170* | 0.051429 | 0.142068* | 0.653991* | 0.532153* | 0.448846* | 0.201952* | 0.688540* | 0.561285* | 0.104418* | 0.162162* |
Mitochondria | 0.012800 | 0.005212 | 0.002371 | 0.028978 | 0.003220 | 0.007732 | 0.001901 | 0.032444 | 0.006287 | 0.006421 | 0.002308 | 0.030141 |
Mitotic Spindle | 0.007102 | 0.017124 | 0.178100* | 0.090792 | 0.015795 | 0.013903 | 0.097339 | 0.034416 | 0.013012 | 0.015578 | 0.167332* | 0.071874 |
None | 0.000179 | 0.000523 | 0.002870 | 0.000125 | 0.001061 | 0.000281 | 0.000050 | 0.000133 | 0.000779 | 0.000407 | 0.002494 | 0.000128 |
Nuclear Periphery | 0.000694 | 0.000423 | 0.007967 | 0.002030 | 0.000261 | 0.000296 | 0.003947 | 0.000896 | 0.000399 | 0.000362 | 0.007431 | 0.001650 |
Nuclear Periphery Foci | 0.013714 | 0.012644 | 0.050147 | 0.004220 | 0.014119 | 0.009975 | 0.015176 | 0.002555 | 0.013989 | 0.011363 | 0.045485 | 0.003661 |
Nucleolus | 0.001424 | 0.000607 | 0.005716 | 0.000357 | 0.001307 | 0.000558 | 0.001123 | 0.000568 | 0.001344 | 0.000584 | 0.005104 | 0.000428 |
Nucleus | 0.000543 | 0.000386 | 0.392410* | 0.000679 | 0.000895 | 0.000301 | 0.000542 | 0.000389 | 0.000782 | 0.000345 | 0.340161* | 0.000582 |
Peroxisomes | 0.065054 | 0.063731 | 0.018031 | 0.128148* | 0.067790 | 0.081280 | 0.054606 | 0.124920* | 0.066914 | 0.072154 | 0.022908 | 0.127065* |
Vacuole | 0.000619 | 0.001569 | 0.032640 | 0.015314 | 0.001106 | 0.002206 | 0.008376 | 0.007536 | 0.000950 | 0.001875 | 0.029405 | 0.012704 |
Vacuole Periphery | 0.000516 | 0.000409 | 0.000510 | 0.004215 | 0.000260 | 0.001252 | 0.001132 | 0.001904 | 0.000342 | 0.000814 | 0.000593 | 0.003440 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.31 | -4.54 | -14.01 | -13.55 | -12.32 | 0.70 | -2.05 | -11.92 | -12.00 | -10.50 | -0.58 | -4.22 | -17.96 | -17.92 | -16.36 |
Bud Neck | -3.89 | -18.90 | -13.77 | -8.48 | 16.49 | -2.20 | -5.43 | -7.75 | -5.14 | 2.61 | -3.89 | -19.03 | -15.17 | -9.89 | 16.60 |
Bud Site | -5.84 | -15.45 | -11.42 | -10.32 | 8.75 | -0.97 | -2.49 | -5.96 | -5.21 | -4.64 | -1.86 | -15.02 | -12.19 | -11.19 | 8.79 |
Cell Periphery | -2.75 | -15.44 | -7.81 | -3.15 | 10.30 | 0.72 | 0.70 | -0.68 | -3.07 | -3.75 | 0.13 | -8.98 | -2.10 | -4.45 | 10.31 |
Cytoplasm | -3.47 | -22.55 | -8.28 | -3.49 | 20.51 | 1.39 | 4.42 | 0.19 | -1.23 | -4.38 | 0.43 | -20.77 | -3.01 | -3.67 | 20.07 |
Cytoplasmic Foci | -18.63 | 0.75 | -33.33 | -14.25 | -33.06* | -5.10 | -7.45 | -13.31 | -9.03 | -0.78 | -12.76 | 6.11 | -29.06 | -17.36 | -31.39 |
Eisosomes | -1.53 | 2.40 | -4.64 | -3.29 | -11.24 | -0.60 | -4.82 | -12.68 | -12.53 | -6.16 | -3.08 | -1.70 | -15.10 | -12.47 | -14.00 |
Endoplasmic Reticulum | -1.91 | -17.34 | -3.81 | -0.20 | 17.05 | 1.21 | -2.66 | -2.73 | -3.61 | 0.03 | -0.55 | -17.04 | -3.59 | -2.28 | 16.75 |
Endosome | -9.11 | 4.37 | -23.72 | -14.82 | -31.65* | -11.18 | -1.19 | -7.58 | 3.58 | -2.97 | -12.56 | 9.96 | -21.93 | -8.23 | -31.04* |
Golgi | -7.02 | 15.58 | -15.25 | -9.03 | -31.39* | -4.66 | -2.08 | -18.07 | -15.09 | -11.37 | -8.18 | 17.86 | -23.33 | -16.82 | -35.29* |
Lipid Particles | 13.07 | 68.95* | 55.53* | 44.07* | -16.53 | 9.45 | 8.81 | 38.12* | 25.22 | 10.48 | 13.71 | 70.65 | 67.23 | 50.14* | -9.58 |
Mitochondria | 2.94 | 3.96 | -4.70 | -9.58 | -11.00 | -3.09 | 1.59 | -7.79 | -6.30 | -8.19 | -0.11 | 3.67 | -10.86 | -11.11 | -13.62 |
Mitotic Spindle | -4.01 | -18.30 | -16.52 | -14.00 | 6.64 | 0.88 | -5.26 | -4.43 | -4.85 | 3.80 | -1.34 | -18.36 | -15.78 | -14.94 | 9.10 |
None | -1.19 | -13.96 | 0.79 | 1.43 | 15.04 | 1.59 | 2.13 | 1.97 | 1.33 | -6.29 | 1.06 | -4.56 | 2.01 | 1.82 | 14.88 |
Nuclear Periphery | 0.63 | -10.68 | -2.35 | -4.12 | 9.09 | -0.63 | -5.07 | -3.10 | -2.92 | 4.05 | 0.27 | -13.71 | -4.19 | -4.81 | 10.55 |
Nuclear Periphery Foci | 0.65 | -8.24 | 3.75 | 4.83 | 12.92 | 2.20 | -0.17 | 6.99 | 4.75 | 3.86 | 1.89 | -9.28 | 7.46 | 6.51 | 13.41 |
Nucleolus | 1.74 | -6.45 | 2.38 | 1.56 | 8.52 | 2.53 | 0.54 | 2.14 | 0.04 | 0.91 | 3.14 | -6.82 | 3.81 | 1.02 | 8.45 |
Nucleus | 0.54 | -37.60 | -0.64 | -1.00 | 37.54 | 1.62 | 0.84 | 1.20 | -0.41 | 0.31 | 1.68 | -35.11 | 0.16 | -1.09 | 35.11 |
Peroxisomes | 0.39 | 9.65 | -8.37 | -9.99 | -21.11* | -2.36 | 1.43 | -6.95 | -5.03 | -6.17 | -1.16 | 13.91 | -11.49 | -10.60 | -23.36* |
Vacuole | -4.58 | -17.68 | -11.33 | -10.69 | 10.11 | -2.20 | -5.28 | -6.97 | -5.36 | 0.54 | -3.40 | -17.71 | -12.62 | -11.61 | 11.25 |
Vacuole Periphery | 0.37 | -0.10 | -6.69 | -8.04 | -7.74 | -2.76 | -3.51 | -6.72 | -1.54 | -2.23 | -2.37 | -2.49 | -9.17 | -7.16 | -8.56 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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