Standard name
Human Ortholog
Description Repressor of G1 transcription; binds to SCB binding factor (SBF) at SCB target promoters in early G1; dilution of Whi5p concentration during cell growth determines cell size; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5, and contributes to both the determination of critical cell size at START and cell fate; periodically expressed in G1

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.12 0.07 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.48 0.5 0.45 0.55 0.69 0.42 0.37 0.38 0.3 0.25 0.16 0.2 0.52 0.63 0.66 0.31 0.32 0.33 0.07 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.12 0.11 0.35 0.2 0.32 0.28 0 0 0 0 0 0 0 0 0
Nucleus 0.45 0.46 0.49 0.38 0.24 0.42 0.42 0.42 0.41 0.48 0.49 0.52 0.48 0.36 0.31 0.42 0.41 0.44 0.57 0.61 0.53
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.06 0 0.1 0.11 0.17 0.14 0 0 0 0.05 0 0 0.16 0.17 0.18
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0.05 0.06 0.06 0.08 0 0 0 0.05 0 0 0 0 0.05 0 0.07 0.09 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 2 1 1 1 0 0 0 0 2 1 0 0 0
Bud 0 1 0 1 1 4 4 1 7 2 6 2 0 0 0 14 45 14 5 3 4
Bud Neck 0 1 1 0 0 0 0 3 3 0 2 2 0 0 0 1 3 1 0 0 4
Bud Site 0 0 1 1 0 1 0 1 6 6 1 5 0 0 0
Cell Periphery 0 1 0 1 3 7 10 3 5 2 3 3 1 1 5 0 0 0 0 0 0
Cytoplasm 57 178 96 132 173 157 129 122 99 77 43 63 99 187 234 37 119 69 13 5 8
Endoplasmic Reticulum 0 0 1 1 2 2 2 3 0 1 1 1 0 0 0 0 0 1 1 3 1
Endosome 1 0 4 5 5 8 8 6 1 0 1 2 2 0 4 2 4 2 4 10 10
Golgi 0 1 1 0 0 0 0 0 0 0 0 1 0 0 1 0 1 1 0 1 2
Mitochondria 1 11 0 9 8 39 42 34 115 61 84 87 0 2 0 1 3 4 1 6 7
Nucleus 54 164 103 92 59 156 147 135 133 147 129 160 92 106 111 50 151 94 104 255 221
Nuclear Periphery 0 1 0 1 0 0 1 0 4 3 6 0 0 0 0 0 1 0 1 3 7
Nucleolus 4 4 1 5 2 10 20 14 31 33 45 42 1 4 4 5 8 7 29 71 77
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 2 0 4 1 6 2 2 1 2 1 1 0 2 6 7 2 12 12
Vac/Vac Membrane 6 12 6 7 13 22 22 27 11 12 11 14 0 3 8 1 18 5 12 37 49
Unique Cell Count 119 357 211 240 250 370 348 320 326 304 262 309 192 298 356 120 369 213 184 420 419
Labelled Cell Count 123 374 216 257 267 410 386 355 419 347 334 385 196 304 367 120 369 213 184 420 419


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 7.3 8.0 7.3 8.0 6.7 6.9 7.0 6.0 6.7 6.1 6.6 7.5 7.7 7.8 8.8 8.5 8.7
Std Deviation (1e-4) 1.1 1.1 1.7 1.1 1.6 1.1 1.4 1.3 1.2 1.4 1.2 1.8 1.1 1.3 1.8 2.3 1.7 2.1
Intensity Change (Log2) -0.14 -0.0 -0.26 -0.21 -0.2 -0.42 -0.26 -0.4 -0.29 -0.1 -0.06 -0.03 0.14 0.08 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.0 5.1 -0.7 -2.0 -1.7 -3.6 -4.8 -6.9 -6.1 1.2 3.9 4.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.9 5.2 4.9 9.7 6.9 9.1 8.4 0 0 0
Nucleus -2.2 -5.6 -1.6 -1.5 -1.5 -1.8 -0.1 0.1 0.7 -0.2 -3.0 -4.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 3.2 2.7 4.3 4.7 6.1 5.3 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.3 1.7 1.8 2.6 0.3 0.7 0.8 1.0 0 0 -0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3521 3.6136 3.4216 3.0261 2.9063 3.5435 5.0727 5.1567 5.2048 5.6874 4.2519 5.1871 2.7554 2.5464 2.7941 2.8738 2.599 2.6672
Actin 0.0122 0.0036 0.018 0.0027 0.001 0.0093 0.0409 0.0017 0.0183 0.0045 0.0583 0.002 0.0025 0.0035 0.0044 0.0033 0.0013 0.002
Bud 0.0005 0.0511 0.0391 0.0422 0.0218 0.0192 0.0016 0.032 0.0341 0.0157 0.0353 0.0307 0.0006 0.0475 0.0476 0.0223 0.0211 0.0372
Bud Neck 0.0011 0.004 0.0043 0.0095 0.0106 0.0252 0.0135 0.0027 0.0057 0.0047 0.0111 0.0225 0.0011 0.0032 0.0053 0.0041 0.0028 0.0184
Bud Periphery 0.0007 0.0287 0.0274 0.0412 0.0203 0.0266 0.0027 0.0157 0.028 0.0108 0.0531 0.0368 0.0003 0.0256 0.0308 0.0208 0.0091 0.0349
Bud Site 0.0054 0.1207 0.039 0.0129 0.0056 0.0075 0.0158 0.0701 0.037 0.0135 0.0231 0.0099 0.0011 0.0974 0.0353 0.0063 0.0033 0.011
Cell Periphery 0.0002 0.0031 0.0024 0.0021 0.0011 0.0018 0.0015 0.0021 0.0024 0.0009 0.0018 0.0012 0.0001 0.0024 0.0021 0.0008 0.0004 0.0011
Cytoplasm 0.0145 0.3671 0.6549 0.577 0.6273 0.109 0.0444 0.3796 0.6179 0.6393 0.4623 0.125 0.0537 0.4704 0.6459 0.6935 0.8276 0.2308
Cytoplasmic Foci 0.0044 0.0087 0.016 0.013 0.0118 0.0063 0.0256 0.003 0.0144 0.0142 0.0579 0.0031 0.0011 0.0043 0.0051 0.0157 0.0253 0.0021
Eisosomes 0.0002 0.0001 0.0002 0 0 0.0001 0.0013 0 0.0002 0.0001 0.0005 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0018 0.0078 0.0078 0.0075 0.0041 0.0018 0.0163 0.0032 0.0054 0.005 0.0048 0.0016 0.0007 0.0092 0.0049 0.0049 0.0039 0.0016
Endosome 0.0099 0.0085 0.0131 0.0292 0.0218 0.0087 0.0333 0.0027 0.0162 0.0456 0.0346 0.0052 0.0015 0.0049 0.0062 0.0285 0.0193 0.0027
Golgi 0.0032 0.0007 0.0041 0.002 0.0014 0.0053 0.0104 0.0003 0.006 0.0155 0.0221 0.002 0.0004 0.0004 0.0006 0.001 0.0007 0.0002
Lipid Particles 0.005 0.0006 0.0057 0.0011 0.0286 0.0037 0.0277 0.0001 0.0027 0.0152 0.0095 0.0067 0.0004 0.0003 0.0006 0.0002 0.0006 0.0001
Mitochondria 0.0089 0.0046 0.0054 0.0023 0.0016 0.004 0.0244 0.001 0.0052 0.005 0.0139 0.0018 0.0006 0.0011 0.0017 0.0022 0.0005 0.0017
None 0.022 0.0393 0.0263 0.0385 0.0289 0.0016 0.0127 0.0138 0.036 0.063 0.0118 0.0025 0.003 0.017 0.0378 0.0113 0.0153 0.003
Nuclear Periphery 0.0081 0.0107 0.0062 0.0053 0.0056 0.0044 0.0118 0.005 0.0043 0.003 0.0058 0.0057 0.0017 0.0084 0.0069 0.011 0.0037 0.0061
Nucleolus 0.0076 0.0105 0.0025 0.0058 0.0038 0.0327 0.0077 0.0043 0.0046 0.001 0.0057 0.0188 0.006 0.0034 0.0024 0.0072 0.001 0.0139
Nucleus 0.8798 0.2958 0.0854 0.1376 0.1716 0.7121 0.6733 0.4444 0.1379 0.0969 0.0995 0.708 0.9201 0.2814 0.1371 0.1389 0.0412 0.6196
Peroxisomes 0.0046 0.0005 0.0091 0.0003 0.001 0.0058 0.0159 0.0001 0.0035 0.0243 0.0326 0.0004 0.0003 0.0003 0.0003 0.0002 0.0003 0.0002
Punctate Nuclear 0.0066 0.0058 0.0068 0.0022 0.0013 0.0026 0.0069 0.002 0.0032 0.0015 0.0234 0.0082 0.0042 0.0034 0.003 0.0054 0.0067 0.0061
Vacuole 0.0022 0.0255 0.0239 0.0629 0.0272 0.0113 0.0099 0.015 0.0153 0.0181 0.0295 0.007 0.0006 0.0143 0.0194 0.0196 0.0143 0.0065
Vacuole Periphery 0.0011 0.0026 0.0025 0.0047 0.0036 0.0013 0.0025 0.0011 0.0018 0.0023 0.0033 0.0009 0.0002 0.0016 0.0025 0.0028 0.0015 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.5322 24.4372 9.0667 9.0424 26.5823 18.6283 14.3197 10.9914 15.8273 16.5153
Translational Efficiency 1.4584 0.9654 0.9657 1.4461 0.9746 1.224 1.48 1.6067 0.987 1.0799

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2376 1377 2621 1234 1645 2085 1447 2097 4021 3462 4068 3331

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 836.69 884.58 999.21 1000.64 776.36 899.21 1054.89 979.72 812.01 893.39 1019.02 987.47
Standard Deviation 111.36 122.53 126.63 131.76 103.57 129.17 136.20 130.12 112.23 126.78 132.82 131.12
Intensity Change Log 2 0.080299 0.256095 0.258158 0.211932 0.442295 0.335644 0.145155 0.348715 0.295972

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000149 0.000604 0.000345 0.000714 0.000248 0.000251 0.000975 0.000730 0.000189 0.000391 0.000569 0.000724
Bud Neck 0.030251 0.035154 0.033296 0.014137 0.024063 0.027738 0.024138 0.013951 0.027719 0.030688 0.030038 0.014020
Bud Site 0.008276 0.018528 0.013286 0.027758 0.010227 0.009409 0.009815 0.020642 0.009074 0.013036 0.012051 0.023278
Cell Periphery 0.000547 0.000454 0.000247 0.000250 0.000449 0.000380 0.000336 0.000204 0.000507 0.000409 0.000279 0.000221
Cytoplasm 0.305212* 0.333833* 0.411329* 0.508211* 0.305315* 0.305326* 0.265895* 0.613872* 0.305254* 0.316664* 0.359598* 0.574729*
Cytoplasmic Foci 0.023510 0.033766 0.009023 0.031066 0.031426 0.019582 0.011587 0.018396 0.026749 0.025224 0.009935 0.023090
Eisosomes 0.000034 0.000032 0.000019 0.000024 0.000029 0.000027 0.000034 0.000017 0.000032 0.000029 0.000024 0.000020
Endoplasmic Reticulum 0.001851 0.003435 0.001326 0.002879 0.002068 0.001771 0.002970 0.001452 0.001940 0.002433 0.001911 0.001981
Endosome 0.005448 0.006720 0.001271 0.005091 0.006065 0.004893 0.002185 0.002680 0.005701 0.005620 0.001596 0.003573
Golgi 0.000487 0.001633 0.000195 0.005143 0.000897 0.000965 0.000799 0.002815 0.000654 0.001231 0.000410 0.003678
Lipid Particles 0.001204 0.000942 0.000218 0.000535 0.000960 0.000726 0.000453 0.000315 0.001104 0.000812 0.000302 0.000397
Mitochondria 0.001937 0.002361 0.001690 0.001403 0.001304 0.002662 0.001365 0.001035 0.001678 0.002542 0.001575 0.001171
Mitotic Spindle 0.002082 0.007853 0.027061 0.047594 0.003637 0.008954 0.017607 0.028678 0.002718 0.008516 0.023699 0.035686
None 0.016123 0.011530 0.010181 0.005844 0.014832 0.013208 0.006838 0.007828 0.015595 0.012541 0.008992 0.007093
Nuclear Periphery 0.000848 0.000668 0.001028 0.000987 0.000506 0.000857 0.000922 0.000731 0.000708 0.000782 0.000990 0.000826
Nuclear Periphery Foci 0.000511 0.001326 0.000543 0.001630 0.000731 0.000458 0.001933 0.000549 0.000601 0.000803 0.001038 0.000950
Nucleolus 0.015118 0.021840 0.005992 0.001854 0.016801 0.015864 0.008336 0.002773 0.015807 0.018241 0.006826 0.002432
Nucleus 0.554691* 0.488161* 0.456701* 0.257992* 0.553970* 0.561719* 0.606908* 0.228882* 0.554396* 0.532462* 0.510130* 0.239666*
Peroxisomes 0.000599 0.001486 0.000187 0.000782 0.000799 0.000973 0.000354 0.000501 0.000681 0.001177 0.000246 0.000605
Vacuole 0.029922 0.027125 0.025626 0.084957 0.024205 0.023154 0.036176 0.052039 0.027583 0.024734 0.029379 0.064234
Vacuole Periphery 0.001201 0.002549 0.000433 0.001148 0.001467 0.001082 0.000374 0.001911 0.001310 0.001665 0.000412 0.001628

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.66 -13.43 -10.42 -3.30 -5.31 0.30 -9.51 -2.83 -2.90 2.65 -6.49 -14.41 -5.99 -3.81 -0.37
Bud Neck -2.42 -8.37 6.76 7.64 14.85 -2.25 -4.60 1.44 3.97 6.33 -2.53 -9.81 5.74 7.97 15.46
Bud Site -8.37 -5.98 -6.29 0.79 -2.13 0.84 -1.66 -4.54 -5.54 -3.55 -5.73 -5.76 -7.91 -3.54 -3.49
Cell Periphery 1.70 9.09 8.94 5.61 1.04 1.60 3.14 6.91 6.21 6.40 2.89 9.26 11.69 8.40 5.22
Cytoplasm -2.10 -2.26 -7.48 -4.67 -5.80 0.07 6.51 -17.54 -18.55 -25.28 -1.16 1.75 -19.68 -17.59 -21.83
Cytoplasmic Foci -3.08 9.68 1.50 4.31 -8.44 4.28 8.93 6.45 2.43 -4.76 1.08 13.25 6.19 4.91 -9.94
Eisosomes 0.54 5.20 -1.39 -1.82 -6.58 0.89 -4.75 3.10 3.14 9.53 1.70 1.05 2.67 1.07 2.11
Endoplasmic Reticulum -5.30 -1.18 -7.78 -2.14 -7.60 1.59 -7.95 -0.35 -1.87 7.25 -3.33 -5.76 -5.71 -2.52 -1.69
Endosome -1.51 10.69 5.38 5.56 -3.23 1.77 7.60 7.45 6.24 0.22 0.34 13.15 9.67 8.42 -2.61
Golgi -3.12 3.71 -1.38 0.11 -1.71 -0.11 1.10 -0.82 -0.70 -1.46 -2.18 2.33 -1.75 -0.43 -2.49
Lipid Particles 1.24 5.61 2.41 0.96 -7.35 1.33 3.50 4.51 2.39 2.72 2.06 6.71 5.02 2.59 -5.03
Mitochondria -0.74 0.20 1.12 2.44 2.45 -2.40 -1.80 0.02 2.54 3.98 -1.96 -0.37 1.19 3.33 4.01
Mitotic Spindle -4.11 -13.52 -10.92 -8.87 -3.85 -3.41 -7.80 -10.90 -8.32 -3.29 -5.50 -15.47 -15.63 -12.07 -4.46
None 3.48 6.40 10.07 6.22 9.04 1.35 8.23 6.63 5.56 -3.13 3.34 9.71 11.35 7.99 4.35
Nuclear Periphery 1.11 -3.21 -5.50 -8.31 -4.22 -3.18 -11.31 -6.16 -1.59 1.29 -1.02 -7.01 -6.29 -5.06 -1.00
Nuclear Periphery Foci -2.73 -0.21 -3.79 0.28 -3.87 2.00 -3.86 1.92 -0.58 5.60 -1.64 -2.91 -2.18 0.20 0.49
Nucleolus -3.97 4.12 11.20 12.04 7.93 0.42 3.16 9.71 9.74 5.53 -2.46 5.31 14.12 15.19 8.86
Nucleus 5.02 4.17 17.12 10.66 14.53 -0.57 -4.31 20.79 23.00 26.26 2.40 0.87 28.18 24.53 28.76
Peroxisomes -4.47 3.89 1.67 5.59 -3.25 -0.55 2.36 2.17 2.26 -0.16 -2.90 4.38 2.64 4.71 -2.35
Vacuole 0.96 -1.51 -18.08 -18.48 -17.45 0.31 -6.39 -14.70 -15.42 -6.33 1.41 -5.30 -22.48 -23.64 -18.12
Vacuole Periphery -2.83 9.43 5.26 4.31 -1.08 2.27 7.58 1.43 0.29 -2.03 -1.64 11.85 2.39 3.08 -2.16