Standard name
Human Ortholog
Description Vacuolar cation channel; mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.17 0.13 0.26 0.22 0.11 0.08 0.07 0 0 0 0 0 0.33 0.34 0.31 0.05 0.15 0.08 0.09 0 0.16 0 0 0
Cytoplasm 0.82 0.89 0.74 0.78 0.86 0.85 0.85 0.89 0.81 0.82 0.76 0.78 0.92 0.86 0.9 0.95 0.87 0.85 0.43 0.71 0.35 0.63 0.67 0.6
Endoplasmic Reticulum 0.09 0 0.16 0.16 0.13 0.08 0.08 0 0.05 0 0 0.07 0 0.09 0.09 0 0 0 0.39 0.16 0.36 0.23 0.23 0.25
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.15 0.22 0.26 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 4 1 1 5 5 4 5 1 1 5 0 0 0 0 2 5 1 2 4 1 1 2
Bud 3 7 5 0 1 2 4 7 8 8 7 5 0 2 0 0 2 0 0 0 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 5 4 2 2 4
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 15 22 31 15 14 16 16 7 5 5 5 8 8 77 57 3 12 8 9 3 21 3 3 1
Cytoplasm 72 151 87 54 113 168 187 186 100 107 162 159 22 193 162 53 71 87 44 136 48 74 145 104
Endoplasmic Reticulum 8 6 19 11 17 15 17 1 6 1 2 14 1 20 16 0 3 1 40 30 50 27 50 42
Endosome 0 0 0 0 0 1 0 1 1 0 3 2 0 0 0 0 0 2 0 0 0 1 1 1
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0
Mitochondria 1 3 0 0 0 5 7 9 19 29 55 30 0 0 0 1 1 1 0 2 2 1 0 0
Nucleus 0 0 1 0 0 3 4 3 1 2 3 1 0 0 0 1 0 2 0 0 0 1 0 1
Nuclear Periphery 1 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 2 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 1 1 3 5 2 10 2 0 6 3 0 0 0 1 3 3 0 4 5 2 5 6
Unique Cell Count 88 169 118 69 131 198 220 209 123 130 212 204 24 224 181 56 82 102 103 191 140 118 216 174
Labelled Cell Count 103 192 149 82 150 220 242 230 148 156 245 229 32 292 235 59 94 110 103 191 140 118 216 174


Cytoplasm, Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.0 7.2 10.1 10.9 10.5 8.0 7.2 6.8 5.1 5.3 5.1 5.3 12.1 11.4 10.8 7.3 8.6 9.3 11.5 11.8 10.9
Std Deviation (1e-4) 2.1 1.5 1.7 2.2 1.6 1.6 1.5 1.3 1.0 1.2 1.0 1.0 2.0 1.6 1.6 1.9 1.4 1.6 1.7 2.1 2.2
Intensity Change (Log2) 0.1 0.05 -0.34 -0.5 -0.57 -1.0 -0.93 -0.99 -0.94 0.26 0.18 0.1 -0.47 -0.24 -0.13 0.19 0.22 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.8 0.7 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.7 -3.2 -4.4 -4.8 -6.2 -4.8 -5.0 -6.7 -5.9 0.7 1.5 1.0 -3.3 -2.0 -3.6
Cytoplasm 0.7 2.5 2.4 2.5 3.6 1.4 1.6 0.5 0.9 1.9 2.8 3.6 3.3 2.2 2.1
Endoplasmic Reticulum 0 -0.7 -2.4 -2.4 -5.7 -2.9 -4.4 -5.4 -2.6 0 -2.0 -1.9 -3.2 -2.8 -3.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.4 5.5 6.1 4.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.0945 10.2838 9.8465 9.1997 9.2048 9.5633 11.2021 13.5026 12.1925 11.4188 11.2297 12.2067 10.3393 11.452 10.7198 10.2476 8.9475 10.4216
Actin 0.0033 0.0016 0.0027 0.0008 0.016 0.0027 0.0455 0.0027 0.0155 0.0133 0.0029 0.0092 0.0179 0.0005 0.0061 0.0015 0.0215 0.0019
Bud 0.0002 0.0003 0.0001 0.0001 0.0002 0.0001 0.0005 0.0001 0.001 0.0007 0.0001 0.0004 0.0007 0.0001 0.0003 0.0002 0.0003 0.0001
Bud Neck 0.0035 0.001 0.0033 0.0038 0.0021 0.0105 0.0025 0.0032 0.004 0.0028 0.0027 0.0075 0.0017 0.0008 0.0035 0.0103 0.0056 0.008
Bud Periphery 0.0004 0.0002 0.0003 0.0002 0.0002 0.0004 0.0009 0.0003 0.0026 0.0021 0.0004 0.0007 0.0019 0.0001 0.001 0.0007 0.0008 0.0003
Bud Site 0.0058 0.0072 0.0012 0.0003 0.0006 0.0002 0.0094 0.0034 0.0105 0.0061 0.0003 0.0005 0.0044 0.0023 0.0059 0.0013 0.0023 0.0002
Cell Periphery 0.0122 0.0097 0.013 0.0274 0.0042 0.0084 0.0206 0.024 0.022 0.0199 0.0136 0.0147 0.024 0.0157 0.0149 0.0214 0.0183 0.0053
Cytoplasm 0.7471 0.8141 0.787 0.8069 0.7811 0.8332 0.6159 0.8661 0.7089 0.6507 0.7907 0.7401 0.7123 0.8843 0.8345 0.8079 0.7757 0.8701
Cytoplasmic Foci 0.0033 0.0018 0.0022 0.0013 0.0058 0.0021 0.0156 0.0024 0.0121 0.0432 0.0139 0.0071 0.0081 0.0008 0.0013 0.0019 0.0233 0.0022
Eisosomes 0.0001 0.0001 0.0001 0 0 0 0.0006 0.0001 0.0002 0.0002 0.0002 0.0001 0.0002 0 0.0001 0 0.0003 0
Endoplasmic Reticulum 0.181 0.1514 0.1696 0.149 0.1483 0.1266 0.1948 0.0756 0.1673 0.1169 0.0841 0.168 0.1577 0.0868 0.1146 0.1135 0.088 0.0954
Endosome 0.0041 0.0006 0.0037 0.0004 0.0065 0.0031 0.0244 0.0016 0.0135 0.0569 0.0168 0.0137 0.0158 0.0002 0.0016 0.0219 0.0169 0.0047
Golgi 0.0024 0.0004 0.0024 0.0002 0.0017 0.0007 0.0124 0.0013 0.009 0.0294 0.0064 0.012 0.0078 0.0001 0.0008 0.0031 0.0111 0.0027
Lipid Particles 0.0092 0.0053 0.006 0.0038 0.0023 0.0023 0.0159 0.0111 0.0083 0.0152 0.0275 0.0057 0.0124 0.0036 0.0022 0.0058 0.0091 0.002
Mitochondria 0.004 0.0001 0.0013 0 0.0004 0.0004 0.0048 0.0001 0.0078 0.0127 0.0009 0.0083 0.0141 0 0.0003 0.001 0.0067 0.0012
None 0.0027 0.002 0.0026 0.0003 0.0019 0.0007 0.0082 0.0038 0.0018 0.0012 0.0191 0.0035 0.003 0.0021 0.0012 0.0007 0.0014 0.0008
Nuclear Periphery 0.0052 0.0015 0.0016 0.002 0.0074 0.0049 0.0118 0.0008 0.002 0.0028 0.0009 0.0019 0.0045 0.0008 0.0013 0.0012 0.0012 0.0016
Nucleolus 0.0008 0.0001 0 0 0.0002 0.0001 0.0005 0 0.0002 0.0004 0.0002 0.0001 0.0001 0 0 0 0.0002 0.0001
Nucleus 0.0044 0.0007 0.0006 0.0007 0.003 0.0011 0.0016 0.0004 0.0017 0.0009 0.0005 0.0011 0.0041 0.0004 0.0006 0.0006 0.0006 0.0011
Peroxisomes 0.002 0 0.0003 0 0.0012 0 0.0032 0.0001 0.0051 0.0078 0.0132 0.0031 0.0022 0 0 0 0.0135 0.0009
Punctate Nuclear 0.0048 0.0004 0.0002 0.0001 0.0158 0.0004 0.0045 0.0001 0.0007 0.0015 0.0027 0.0005 0.001 0.0001 0.0001 0.0001 0.0012 0.0005
Vacuole 0.0032 0.0013 0.002 0.0026 0.0009 0.0017 0.0051 0.0027 0.0052 0.014 0.0026 0.0019 0.0053 0.0012 0.0071 0.0061 0.0015 0.001
Vacuole Periphery 0.0002 0 0.0001 0 0.0001 0.0003 0.0014 0 0.0006 0.0012 0.0002 0.0003 0.0009 0 0.0025 0.0008 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.5946 18.4824 20.933 21.9721 14.814 27.1892 23.8512 25.7235 24.3427 21.955
Translational Efficiency 0.6336 0.7161 0.7047 0.6863 0.7137 0.4986 0.7632 0.5741 0.7198 0.5797

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency