Standard name
Human Ortholog
Description Alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.27 0.31 0.52 0.39 0.42 0.48 0.44 0.53 0.5 0.59 0.61 0.57 0.75 0.91 0.14 0.12 0.22 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Endosome 0.06 0.05 0.09 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0.05 0.08 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.17 0 0.06 0.11 0.15 0.16 0.14 0.13 0.15 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.18 0.1 0.08 0.06 0.08 0.18 0.11 0.12 0.07 0.1 0.08 0.06 0.06 0 0 0.5 0.37 0.43 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.81 0.79 0.6 0.69 0.66 0.61 0.56 0.51 0.57 0.54 0.42 0.43 0.47 0.34 0.14 0.52 0.66 0.5 0.87 0.89 0.89 0.87 0.81 0.85
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 3 0 3 4 3 9 2 4 5 9 1 2 1 0 0 0 1 3 1 1 4 0
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Cytoplasm 44 60 143 150 185 275 375 371 386 408 403 507 141 299 402 16 15 35 10 5 5 5 3 5
Endoplasmic Reticulum 3 1 1 0 0 3 4 4 1 0 1 1 9 1 3 4 7 8 0 0 2 2 6 3
Endosome 25 10 42 8 22 2 4 2 2 3 1 2 5 4 2 3 2 4 28 6 27 11 29 21
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 1 1
Mitochondria 17 5 81 8 31 74 119 135 100 104 104 140 0 2 0 3 2 2 3 1 7 3 9 6
Nucleus 74 22 39 18 36 118 83 97 54 81 55 54 14 12 4 58 45 69 6 3 3 1 4 4
Nuclear Periphery 1 0 2 2 2 5 13 10 5 6 2 6 0 0 0 0 0 0 2 0 1 1 4 4
Nucleolus 0 0 1 0 2 1 2 2 1 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 3 2 0 0 0 0 0 1 0 1 0 0 0 1 1 3 2 1 1 0 1 0
Vac/Vac Membrane 332 173 279 198 318 400 438 431 415 443 288 360 115 137 64 60 80 81 377 215 448 222 299 302
Unique Cell Count 412 219 467 286 479 655 783 838 722 814 682 833 246 401 443 115 122 162 436 243 506 255 370 357
Labelled Cell Count 497 271 595 386 600 882 1041 1062 966 1050 860 1080 286 457 476 146 154 204 436 243 506 255 370 357


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 5.7 4.7 5.3 5.6 5.3 4.8 4.8 5.0 4.9 5.0 5.0 5.9 5.2 5.2 7.6 8.5 8.8 7.4 7.1 7.6
Std Deviation (1e-4) 1.1 1.1 1.4 1.5 1.5 1.4 1.6 1.3 1.4 1.4 1.4 1.3 1.2 1.1 1.2 2.0 2.5 3.5 1.7 1.5 1.6
Intensity Change (Log2) 0.15 0.25 0.17 0.03 0.01 0.09 0.05 0.09 0.07 0.33 0.14 0.14 0.67 0.84 0.89 0.65 0.59 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6.0 2.6 3.9 6.0 4.8 7.7 6.8 9.5 10.5 6.9 12.9 18.5 -3.6 -4.1 -2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -3.3 -2.7 -7.4 0 0 -7.8 -8.1 -7.8 -8.4 -3.6 -5.2 -6.0 -2.3 -2.8 -2.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -6.0 -5.2 -2.9 0 0 -1.6 -2.2 -0.9 -0.2 -6.9 -8.4 -9.2 -4.0 -4.4 -5.2
Nucleus -1.0 -0.5 4.6 1.3 1.8 -0.5 0.9 -0.2 -1.3 -1.3 -3.3 -5.3 10.8 8.0 10.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.6 2.1 0.4 -1.3 -2.9 -0.8 -1.8 -5.8 -5.7 -3.3 -7.5 -14.1 -1.5 1.2 -2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5835 3.2603 3.0512 2.5571 2.3751 3.1471 4.2857 6.4383 5.6198 5.3869 5.3809 5.9008 2.2998 5.0165 3.7655 3.8261 3.8496 4.0488
Actin 0.0043 0 0.0003 0 0.0001 0.0001 0.0058 0 0.0005 0 0 0.0003 0.0082 0 0.0033 0 0 0
Bud 0.0006 0.0005 0.0004 0.0002 0.0014 0.0007 0.0006 0.0002 0.0004 0.0004 0.0002 0.0003 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002
Bud Neck 0.0031 0.0013 0.0022 0.0004 0.0014 0.0013 0.001 0.0008 0.0012 0.0008 0.0008 0.0016 0.0008 0.0006 0.001 0.001 0.0012 0.0011
Bud Periphery 0.0015 0.0013 0.0009 0.0007 0.0053 0.0015 0.0018 0.001 0.0016 0.0013 0.0006 0.0019 0.0008 0.0006 0.0007 0.0006 0.0006 0.0012
Bud Site 0.0082 0.0012 0.0021 0.0003 0.0011 0.0006 0.0012 0.0005 0.001 0.0005 0.0003 0.0006 0.0005 0.0005 0.0004 0.0004 0.0009 0.0007
Cell Periphery 0.0024 0.0032 0.0039 0.0021 0.0036 0.0039 0.0043 0.0073 0.005 0.0038 0.0044 0.0071 0.0033 0.0037 0.0032 0.004 0.0052 0.0055
Cytoplasm 0.0041 0.0041 0.0088 0.0011 0.0037 0.0016 0.0035 0.0017 0.0026 0.0005 0.0006 0.0014 0.0021 0.0039 0.0007 0.0004 0.0005 0.0007
Cytoplasmic Foci 0.0315 0.0092 0.0184 0.0073 0.0117 0.0107 0.0124 0.004 0.0069 0.0008 0.0085 0.0041 0.005 0.0025 0.0031 0.0005 0.0025 0.0011
Eisosomes 0.0002 0 0 0 0 0 0.0003 0 0 0 0 0 0.0004 0 0 0 0 0
Endoplasmic Reticulum 0.0043 0.0026 0.0083 0.0005 0.0034 0.0015 0.0025 0.004 0.0037 0.0007 0.0017 0.002 0.0029 0.0029 0.0014 0.0007 0.0007 0.0018
Endosome 0.2543 0.1085 0.2081 0.2043 0.2048 0.0857 0.1549 0.0995 0.1394 0.0815 0.0982 0.1077 0.1907 0.0969 0.1467 0.0592 0.1028 0.0734
Golgi 0.0285 0.0032 0.0087 0.0059 0.008 0.0045 0.0078 0.0043 0.0097 0.0022 0.0045 0.0059 0.0077 0.0036 0.0084 0.0011 0.0044 0.0024
Lipid Particles 0.0598 0.0119 0.0121 0.0055 0.0135 0.0182 0.0224 0.0169 0.0322 0.0062 0.0144 0.0209 0.0351 0.0076 0.0167 0.0029 0.0151 0.0103
Mitochondria 0.0385 0.003 0.0191 0.0032 0.0044 0.0182 0.0133 0.0125 0.0299 0.0147 0.0104 0.0123 0.0227 0.0138 0.0201 0.0012 0.0011 0.0057
None 0.0177 0.0002 0.0009 0.0001 0.0032 0.0007 0.0126 0.0001 0.0032 0 0.0001 0.0013 0.0037 0.0002 0.0001 0 0 0.0001
Nuclear Periphery 0.0112 0.014 0.0109 0.0011 0.0025 0.0031 0.0065 0.0047 0.0045 0.001 0.0024 0.0038 0.0114 0.005 0.0023 0.0015 0.0012 0.0103
Nucleolus 0.0041 0.0035 0.0055 0.0017 0.0055 0.004 0.0048 0.002 0.0052 0.0022 0.002 0.0028 0.0041 0.0021 0.0027 0.0031 0.0042 0.0046
Nucleus 0.0038 0.006 0.0048 0.0017 0.0054 0.004 0.0038 0.0021 0.0029 0.002 0.0016 0.003 0.0031 0.0023 0.0018 0.002 0.0021 0.0042
Peroxisomes 0.0182 0.0008 0.0026 0.0007 0.001 0.0013 0.0046 0.0004 0.007 0.0005 0.0011 0.0017 0.003 0.0002 0.0032 0.0002 0.0008 0.0004
Punctate Nuclear 0.0021 0.0001 0.0003 0.0001 0.0003 0.0001 0.0007 0.0001 0.0008 0 0.0002 0.0018 0.0016 0.0001 0.0001 0 0 0.0007
Vacuole 0.3533 0.6467 0.5191 0.5916 0.6059 0.6738 0.5222 0.6343 0.5546 0.6793 0.6621 0.624 0.4866 0.6147 0.5461 0.6981 0.6828 0.6528
Vacuole Periphery 0.1485 0.1787 0.1627 0.1718 0.1139 0.1646 0.2129 0.2037 0.1878 0.2016 0.1861 0.1955 0.2061 0.2384 0.2378 0.2232 0.1737 0.2228

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 182.5005 141.0301 126.9428 119.9302 144.0295 160.4742 146.8142 131.5501 118.7571 163.5304
Translational Efficiency 1.0532 1.1722 0.9706 1.1762 1.1865 1.0969 1.0876 1.063 0.9064 0.9833

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1480 1596 118 992 1920 124 136 1064 3400 1720 254 2056

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 669.45 842.70 922.14 907.98 748.92 806.90 963.42 959.22 714.33 840.12 944.24 934.50
Standard Deviation 78.38 122.99 100.01 144.88 100.19 95.04 99.37 141.04 99.47 121.54 101.77 145.18
Intensity Change Log 2 0.332043 0.462009 0.439684 0.107578 0.363353 0.357050 0.217882 0.410759 0.396643

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000046 0.000045 0.001069 0.001669 0.000053 0.000083 0.000396 0.001950 0.000050 0.000048 0.000709 0.001815
Bud Neck 0.029285 0.011398 0.006530 0.020374 0.014658 0.022558 0.016385 0.020572 0.021025 0.012202 0.011807 0.020477
Bud Site 0.011077 0.010434 0.019528 0.053053 0.007908 0.005554 0.008377 0.050295 0.009287 0.010082 0.013557 0.051626
Cell Periphery 0.000323 0.000374 0.000140 0.000142 0.000228 0.000976 0.000109 0.000159 0.000269 0.000417 0.000124 0.000151
Cytoplasm 0.072855 0.024119 0.092772 0.099903 0.053399 0.017897 0.053581 0.105555* 0.061868 0.023670 0.071788 0.102828*
Cytoplasmic Foci 0.064795 0.016519 0.139693 0.082409 0.033112 0.026158 0.047793 0.119265 0.046903 0.017214 0.090487 0.101482
Eisosomes 0.000074 0.000016 0.000069 0.000024 0.000019 0.000067 0.000070 0.000033 0.000043 0.000019 0.000069 0.000029
Endoplasmic Reticulum 0.004929 0.003206 0.008376 0.001114 0.001677 0.002087 0.004913 0.001562 0.003092 0.003125 0.006522 0.001346
Endosome 0.219004* 0.067061 0.239041* 0.202207* 0.091776 0.069290 0.279183* 0.280627* 0.147158 0.067222 0.260534* 0.242790*
Golgi 0.002819 0.002208 0.003199 0.010564 0.001898 0.002115 0.001109 0.009671 0.002299 0.002201 0.002080 0.010102
Lipid Particles 0.009636 0.001087 0.019338 0.002690 0.001692 0.013026 0.010708 0.003665 0.005150 0.001947 0.014717 0.003195
Mitochondria 0.004486 0.001294 0.000408 0.005391 0.002154 0.018306 0.000358 0.003571 0.003169 0.002521 0.000381 0.004449
Mitotic Spindle 0.000232 0.001086 0.013390 0.020831 0.000347 0.000297 0.018748 0.016732 0.000297 0.001029 0.016259 0.018710
None 0.000381 0.000199 0.000478 0.000262 0.000167 0.000098 0.000262 0.000258 0.000260 0.000192 0.000362 0.000260
Nuclear Periphery 0.000729 0.000180 0.001776 0.000396 0.000240 0.000993 0.002870 0.000454 0.000453 0.000239 0.002362 0.000426
Nuclear Periphery Foci 0.000227 0.000174 0.018597 0.001396 0.000113 0.000334 0.015817 0.001930 0.000163 0.000186 0.017109 0.001672
Nucleolus 0.001045 0.000652 0.002705 0.001136 0.000513 0.002658 0.010596 0.000953 0.000745 0.000796 0.006930 0.001041
Nucleus 0.018750 0.003796 0.034067 0.006559 0.006701 0.011499 0.039258 0.005993 0.011946 0.004351 0.036846 0.006266
Peroxisomes 0.003126 0.000948 0.016902 0.014787 0.001288 0.005764 0.015503 0.026301 0.002088 0.001295 0.016153 0.020746
Vacuole 0.537312* 0.811832* 0.376977* 0.465264* 0.762193* 0.784830* 0.470036* 0.344707* 0.664304* 0.809885* 0.426804* 0.402874*
Vacuole Periphery 0.018868 0.043374 0.004945 0.009828 0.019866 0.015408 0.003928 0.005747 0.019432 0.041358 0.004401 0.007716

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.10 -2.06 -2.46 -2.46 -0.50 -0.86 -3.31 -3.75 -3.68 -2.77 -0.00 -2.88 -4.31 -4.31 -2.00
Bud Neck 8.29 10.45 3.62 -5.30 -8.05 -1.18 -0.21 -2.76 0.38 -1.36 6.30 4.88 0.57 -5.60 -4.41
Bud Site 0.35 -0.94 -9.82 -10.42 -3.90 0.84 -0.27 -11.56 -9.42 -10.81 -0.77 -1.01 -15.18 -14.54 -8.31
Cell Periphery -1.23 5.25 5.28 5.64 0.37 -3.25 3.67 2.60 3.68 -0.67 -3.69 6.10 5.28 7.20 -0.17
Cytoplasm 11.82 -1.92 -7.97 -16.16 -1.39 6.07 -1.00 -11.29 -14.14 -4.34 12.69 -2.13 -13.86 -22.25 -3.99
Cytoplasmic Foci 15.56 -5.13 -2.55 -15.38 4.24 0.74 -2.36 -16.38 -8.83 -6.98 13.48 -5.41 -14.05 -24.25 -0.18
Eisosomes 10.67 -0.10 8.88 -3.32 6.26 -2.64 -6.58 -4.70 1.85 4.82 7.47 -5.20 4.71 -3.59 7.67
Endoplasmic Reticulum 2.93 -1.17 7.13 5.22 2.56 -1.24 -4.16 0.82 1.69 4.32 -0.10 -2.68 6.17 5.23 4.01
Endosome 22.02* 0.23 6.05 -12.90 2.20 1.66 -6.26 -15.44 -10.11 0.71 17.78 -4.89 -7.48 -21.20 1.99
Golgi 0.99 0.03 -3.12 -3.51 -3.04 -0.24 2.42 -4.31 -3.44 -5.15 0.19 1.21 -5.21 -5.09 -5.62
Lipid Particles 9.93 -2.33 7.73 -6.75 4.07 -1.57 -4.17 -5.31 1.25 3.14 4.67 -4.34 3.65 -2.59 5.12
Mitochondria 3.80 4.89 0.20 -2.46 -3.22 -1.66 3.36 -0.76 1.59 -3.38 0.76 5.90 -0.39 -0.98 -4.54
Mitotic Spindle -1.45 -2.33 -7.36 -6.99 -1.60 0.18 -1.82 -7.29 -7.31 -0.34 -1.33 -2.72 -10.34 -9.70 -1.13
None 1.77 -0.85 0.46 -1.02 1.09 1.06 -1.50 -1.62 -2.38 0.16 0.99 -1.56 -0.75 -1.54 0.99
Nuclear Periphery 5.11 -2.75 0.22 -6.18 2.81 -1.59 -4.11 -2.99 0.72 3.60 2.52 -4.97 -2.34 -5.76 4.56
Nuclear Periphery Foci 1.25 -4.74 -5.66 -5.80 4.33 -3.06 -4.34 -5.01 -4.58 3.66 -0.65 -6.42 -7.13 -7.03 5.64
Nucleolus 2.09 -1.59 -1.41 -3.14 1.41 -1.30 -2.60 -2.98 0.94 2.43 -0.42 -3.02 -3.30 -1.92 2.78
Nucleus 8.53 -2.43 4.11 -6.24 3.37 -1.70 -4.32 -2.43 0.69 4.05 7.85 -4.98 1.74 -7.01 5.26
Peroxisomes 5.35 -2.91 -6.09 -7.73 0.80 -2.34 -3.04 -11.28 -5.99 -1.89 2.71 -4.23 -12.71 -13.32 -0.82
Vacuole -27.44 5.69 4.15 29.13* -3.66 -0.77 10.65 32.86 13.34 3.10 -18.85 11.91 26.11 42.38* -0.15
Vacuole Periphery -11.75 9.28 8.83 17.28 -2.24 1.28 16.67 14.37 3.17 -1.51 -11.70 17.31 16.20 19.18 -2.75