Standard name
Description Exomer-dependent cargo protein; induces appearance of [PIN+] prion when overproduced; prion-like domain serves as a retention signal in the trans-Golgi network; predicted to be palmitoylated
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.46 0.24 0.35 0.3 0.12 0.12 0.09 0.11 0.09 0.07 0.11 0.1 0.27 0.19 0.15 0.07 0.15 0.11 0.19 0.14 0.2 0.11 0.14 0.09
Bud 0.12 0.17 0.13 0.12 0 0.06 0.07 0.07 0.1 0.07 0.08 0.08 0.09 0 0.05 0 0 0 0.11 0.11 0.09 0.12 0.11 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.12 0.15 0.12 0.06
Bud Site 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.23 0.38 0.32 0.49 0.55 0.58 0.63 0.55 0.61 0.64 0.61 0.59 0.39 0.65 0.64 0.22 0.11 0.21 0 0.07 0 0.12 0.12 0.16
Endoplasmic Reticulum 0.35 0.18 0.2 0.06 0.06 0.08 0 0 0 0.05 0.09 0 0.36 0.19 0.16 0 0.05 0.07 0.15 0.09 0.12 0.08 0.15 0.17
Endosome 0 0 0 0 0.08 0.11 0.11 0.12 0.06 0.09 0.05 0.12 0 0 0 0.22 0.21 0.12 0.05 0.06 0.06 0.07 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.21 0.15 0.05 0 0 0 0 0
Mitochondria 0 0.21 0 0 0 0 0 0 0.07 0 0.06 0.08 0.07 0 0 0.09 0.05 0.05 0.05 0.09 0.06 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.09 0.07 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.05 0.1 0.05 0.27 0.17 0.17 0.21 0.16 0.15 0.13 0.16 0 0 0 0.37 0.37 0.4 0.29 0.31 0.24 0.22 0.29 0.35
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 12 37 108 95 46 58 59 80 43 47 46 57 12 25 24 12 23 17 16 28 93 10 29 17
Bud 3 26 39 39 16 28 45 56 47 48 32 47 4 6 8 1 1 3 9 23 41 11 22 11
Bud Neck 0 0 7 1 1 0 0 1 0 0 0 0 0 1 0 0 0 0 3 16 54 13 24 10
Bud Site 0 3 5 17 0 6 6 0 4 4 1 3 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 1 6 6 3 3 6 7 6 5 9 5 1 8 5 3 3 4 0 0 3 0 1 0
Cytoplasm 6 59 98 157 211 283 434 416 301 430 256 345 17 88 105 38 17 31 3 15 17 11 24 31
Endoplasmic Reticulum 9 28 62 19 22 38 20 30 22 31 37 24 16 26 26 7 7 11 13 19 57 7 30 32
Endosome 0 0 4 14 31 54 75 87 31 64 22 68 1 2 7 37 31 18 4 12 27 6 5 6
Golgi 0 1 2 1 2 1 1 0 0 2 0 0 0 0 0 15 32 22 4 4 12 2 0 2
Mitochondria 1 33 0 6 0 2 5 6 36 28 24 47 3 0 2 16 8 7 4 18 26 5 5 7
Nucleus 0 1 3 0 0 5 5 4 3 3 5 5 0 0 0 0 1 1 0 1 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 1 3 0 0 0 0 0 0 0 0 0 0 0 1 3 1 0 1
Nucleolus 0 0 0 1 0 1 2 4 1 1 3 4 0 0 1 3 2 4 0 0 0 0 0 0
Peroxisomes 0 0 4 10 1 6 14 4 0 5 1 2 0 2 2 1 0 1 0 0 3 0 0 0
SpindlePole 0 0 28 23 12 11 13 20 6 13 5 11 0 3 7 12 10 18 0 0 2 0 0 0
Vac/Vac Membrane 3 8 30 15 103 81 120 159 77 104 53 94 0 0 0 62 55 60 25 64 112 20 60 68
Unique Cell Count 26 156 311 319 386 488 689 750 490 674 422 584 44 135 165 169 150 150 87 210 465 94 208 196
Labelled Cell Count 34 197 396 404 448 577 806 877 577 785 494 712 54 162 187 207 190 197 87 210 465 94 208 196


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 5.7 8.3 7.7 8.4 7.5 7.7 7.9 6.6 7.4 7.3 7.3 6.8 7.4 6.4 8.4 10.4 11.6 10.6 10.8 10.6
Std Deviation (1e-4) 1.4 0.9 1.4 1.5 1.4 1.4 1.4 1.2 1.2 1.2 1.2 1.3 1.2 3.1 1.4 1.4 1.8 2.6 8.1 8.4 7.8
Intensity Change (Log2) – – – -0.1 0.03 -0.14 -0.11 -0.08 -0.32 -0.16 -0.19 -0.18 -0.3 -0.17 -0.37 0.02 0.33 0.48 0.35 0.39 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.3 -7.2 -7.8 -10.3 -9.3 -9.2 -11.1 -7.8 -9.2 -1.0 -3.4 -4.7 -6.7 -4.3 -5.3
Bud -0.1 -4.1 -3.4 -3.2 -2.6 -1.3 -2.8 -2.2 -2.2 -0.7 -2.6 -2.7 -4.5 -4.2 -3.7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 2.5 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0.2 0 0 0 0 0 0 0
Cytoplasm 4.5 6.1 7.3 9.2 7.1 8.3 9.4 7.8 7.9 0.9 6.6 6.7 -2.1 -4.7 -2.4
Endoplasmic Reticulum -5.2 -5.7 -5.1 -9.1 -8.4 -7.0 -7.7 -4.4 -7.6 2.5 -0.2 -1.1 -4.7 -4.3 -3.5
Endosome 2.3 4.1 5.2 5.2 5.5 3.4 4.7 2.8 5.4 0 0 0 7.7 7.4 5.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 4.7 8.0 6.4
Mitochondria 0 0 0 0 0 4.9 3.6 4.3 5.1 0 0 0 5.5 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1.6 0 0 0.8 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -0.8 -3.3 -4.3 -5.3 -4.5 -5.3 -5.2 -5.0 -5.0 0 -2.6 -1.9 -0.7 -0.9 1.0
Vacuole -2.4 5.7 2.8 3.2 4.5 2.5 2.5 1.2 2.7 0 -3.7 -4.1 7.2 7.0 7.7