Standard name | |
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Description | Exomer-dependent cargo protein; induces appearance of [PIN+] prion when overproduced; prion-like domain serves as a retention signal in the trans-Golgi network; predicted to be palmitoylated |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 12 | 37 | 108 | 95 | 46 | 58 | 59 | 80 | 43 | 47 | 46 | 57 | 12 | 25 | 24 | 12 | 23 | 17 | 16 | 28 | 93 | 10 | 29 | 17 |
Bud | 3 | 26 | 39 | 39 | 16 | 28 | 45 | 56 | 47 | 48 | 32 | 47 | 4 | 6 | 8 | 1 | 1 | 3 | 9 | 23 | 41 | 11 | 22 | 11 |
Bud Neck | 0 | 0 | 7 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 16 | 54 | 13 | 24 | 10 |
Bud Site | 0 | 3 | 5 | 17 | 0 | 6 | 6 | 0 | 4 | 4 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 6 | 6 | 3 | 3 | 6 | 7 | 6 | 5 | 9 | 5 | 1 | 8 | 5 | 3 | 3 | 4 | 0 | 0 | 3 | 0 | 1 | 0 |
Cytoplasm | 6 | 59 | 98 | 157 | 211 | 283 | 434 | 416 | 301 | 430 | 256 | 345 | 17 | 88 | 105 | 38 | 17 | 31 | 3 | 15 | 17 | 11 | 24 | 31 |
Endoplasmic Reticulum | 9 | 28 | 62 | 19 | 22 | 38 | 20 | 30 | 22 | 31 | 37 | 24 | 16 | 26 | 26 | 7 | 7 | 11 | 13 | 19 | 57 | 7 | 30 | 32 |
Endosome | 0 | 0 | 4 | 14 | 31 | 54 | 75 | 87 | 31 | 64 | 22 | 68 | 1 | 2 | 7 | 37 | 31 | 18 | 4 | 12 | 27 | 6 | 5 | 6 |
Golgi | 0 | 1 | 2 | 1 | 2 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 15 | 32 | 22 | 4 | 4 | 12 | 2 | 0 | 2 |
Mitochondria | 1 | 33 | 0 | 6 | 0 | 2 | 5 | 6 | 36 | 28 | 24 | 47 | 3 | 0 | 2 | 16 | 8 | 7 | 4 | 18 | 26 | 5 | 5 | 7 |
Nucleus | 0 | 1 | 3 | 0 | 0 | 5 | 5 | 4 | 3 | 3 | 5 | 5 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 4 | 1 | 1 | 3 | 4 | 0 | 0 | 1 | 3 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 4 | 10 | 1 | 6 | 14 | 4 | 0 | 5 | 1 | 2 | 0 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 28 | 23 | 12 | 11 | 13 | 20 | 6 | 13 | 5 | 11 | 0 | 3 | 7 | 12 | 10 | 18 | 0 | 0 | 2 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | 8 | 30 | 15 | 103 | 81 | 120 | 159 | 77 | 104 | 53 | 94 | 0 | 0 | 0 | 62 | 55 | 60 | 25 | 64 | 112 | 20 | 60 | 68 |
Unique Cell Count | 26 | 156 | 311 | 319 | 386 | 488 | 689 | 750 | 490 | 674 | 422 | 584 | 44 | 135 | 165 | 169 | 150 | 150 | 87 | 210 | 465 | 94 | 208 | 196 |
Labelled Cell Count | 34 | 197 | 396 | 404 | 448 | 577 | 806 | 877 | 577 | 785 | 494 | 712 | 54 | 162 | 187 | 207 | 190 | 197 | 87 | 210 | 465 | 94 | 208 | 196 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.9 | 5.7 | 8.3 | 7.7 | 8.4 | 7.5 | 7.7 | 7.9 | 6.6 | 7.4 | 7.3 | 7.3 | 6.8 | 7.4 | 6.4 | 8.4 | 10.4 | 11.6 | 10.6 | 10.8 | 10.6 |
Std Deviation (1e-4) | 1.4 | 0.9 | 1.4 | 1.5 | 1.4 | 1.4 | 1.4 | 1.2 | 1.2 | 1.2 | 1.2 | 1.3 | 1.2 | 3.1 | 1.4 | 1.4 | 1.8 | 2.6 | 8.1 | 8.4 | 7.8 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.03 | -0.14 | -0.11 | -0.08 | -0.32 | -0.16 | -0.19 | -0.18 | -0.3 | -0.17 | -0.37 | 0.02 | 0.33 | 0.48 | 0.35 | 0.39 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.3 | -7.2 | -7.8 | -10.3 | -9.3 | -9.2 | -11.1 | -7.8 | -9.2 | -1.0 | -3.4 | -4.7 | -6.7 | -4.3 | -5.3 |
Bud | -0.1 | -4.1 | -3.4 | -3.2 | -2.6 | -1.3 | -2.8 | -2.2 | -2.2 | -0.7 | -2.6 | -2.7 | -4.5 | -4.2 | -3.7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4.5 | 6.1 | 7.3 | 9.2 | 7.1 | 8.3 | 9.4 | 7.8 | 7.9 | 0.9 | 6.6 | 6.7 | -2.1 | -4.7 | -2.4 |
Endoplasmic Reticulum | -5.2 | -5.7 | -5.1 | -9.1 | -8.4 | -7.0 | -7.7 | -4.4 | -7.6 | 2.5 | -0.2 | -1.1 | -4.7 | -4.3 | -3.5 |
Endosome | 2.3 | 4.1 | 5.2 | 5.2 | 5.5 | 3.4 | 4.7 | 2.8 | 5.4 | 0 | 0 | 0 | 7.7 | 7.4 | 5.1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.7 | 8.0 | 6.4 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.9 | 3.6 | 4.3 | 5.1 | 0 | 0 | 0 | 5.5 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.6 | 0 | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -0.8 | -3.3 | -4.3 | -5.3 | -4.5 | -5.3 | -5.2 | -5.0 | -5.0 | 0 | -2.6 | -1.9 | -0.7 | -0.9 | 1.0 |
Vacuole | -2.4 | 5.7 | 2.8 | 3.2 | 4.5 | 2.5 | 2.5 | 1.2 | 2.7 | 0 | -3.7 | -4.1 | 7.2 | 7.0 | 7.7 |