| Standard name | |
|---|---|
| Description | Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 4 | 7 | 5 | 1 | 5 | 3 |
| Bud | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 1 | 8 | 5 | 4 | 10 | 0 | 0 | 0 | – | – | 0 | 0 | 3 | 3 | 1 | 4 | 3 |
| Bud Neck | 1 | 2 | 4 | 4 | 1 | 14 | 20 | 11 | 4 | 4 | 2 | 5 | 1 | 2 | 0 | – | – | 0 | 1 | 3 | 2 | 0 | 1 | 1 |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | – | – |
| Cell Periphery | 1 | 5 | 2 | 2 | 0 | 0 | 0 | 1 | 3 | 1 | 1 | 1 | 0 | 2 | 1 | – | – | 1 | 1 | 4 | 4 | 1 | 2 | 1 |
| Cytoplasm | 348 | 512 | 239 | 165 | 107 | 275 | 300 | 293 | 291 | 268 | 194 | 195 | 282 | 283 | 294 | – | – | 218 | 308 | 430 | 155 | 257 | 241 | 318 |
| Endoplasmic Reticulum | 1 | 5 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 12 | 0 | 2 | 4 | 0 | 1 | 0 |
| Endosome | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | 2 | 5 | 6 | 7 | 1 | 2 | 3 |
| Golgi | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | 0 | 0 | 1 | 3 | 0 | 3 | 0 |
| Mitochondria | 7 | 9 | 2 | 1 | 7 | 15 | 15 | 16 | 92 | 68 | 73 | 71 | 1 | 0 | 0 | – | – | 6 | 17 | 23 | 41 | 8 | 15 | 9 |
| Nucleus | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 6 | 1 | 1 | 0 | 4 | 1 | 1 | 0 | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 1 | 0 | 1 | 0 | 1 |
| Nucleolus | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
| Peroxisomes | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
| SpindlePole | 1 | 3 | 1 | 1 | 0 | 4 | 6 | 5 | 2 | 3 | 1 | 4 | 0 | 0 | 0 | – | – | 1 | 3 | 7 | 2 | 1 | 2 | 2 |
| Vac/Vac Membrane | 2 | 1 | 1 | 0 | 1 | 0 | 3 | 1 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | – | – | 4 | 7 | 2 | 3 | 2 | 2 | 2 |
| Unique Cell Count | 355 | 529 | 247 | 168 | 113 | 288 | 312 | 316 | 338 | 316 | 236 | 250 | 284 | 283 | 295 | 222 | 359 | 509 | 258 | 285 | 294 | 361 | ||
| Labelled Cell Count | 363 | 541 | 256 | 176 | 120 | 310 | 348 | 339 | 406 | 352 | 275 | 296 | 286 | 288 | 296 | 244 | 359 | 509 | 258 | 285 | 294 | 361 | ||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 3.9 | 3.6 | 5.0 | 4.4 | 3.8 | 4.1 | 4.1 | 4.6 | 3.3 | 3.7 | 3.3 | 3.5 | 4.4 | 4.6 | 4.4 | 4.0 | 4.8 | 4.9 | 4.7 | 4.9 | 5.0 |
| Std Deviation (1e-4) | 0.9 | 0.7 | 1.9 | 2.2 | 1.7 | 1.7 | 1.7 | 2.3 | 1.6 | 1.0 | 1.0 | 0.9 | 1.0 | 1.0 | 1.5 | 1.1 | 1.1 | 1.2 | 1.3 | 1.4 | 1.2 |
| Intensity Change (Log2) | – | – | – | -0.16 | -0.38 | -0.27 | -0.28 | -0.11 | -0.57 | -0.45 | -0.6 | -0.53 | -0.17 | -0.12 | -0.17 | -0.32 | -0.05 | -0.02 | -0.07 | -0.01 | 0.02 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
