Standard name
Description Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.97 0.98 0.95 0.95 0.96 0.93 0.86 0.85 0.82 0.78 0.99 1.0 1.0 0.98 0.86 0.85 0.6 0.9 0.82 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.05 0.05 0.05 0.27 0.22 0.31 0.28 0 0 0 0 0.05 0.05 0.16 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 4 7 5 1 5 3
Bud 0 0 1 1 2 0 1 1 8 5 4 10 0 0 0 0 0 3 3 1 4 3
Bud Neck 1 2 4 4 1 14 20 11 4 4 2 5 1 2 0 0 1 3 2 0 1 1
Bud Site 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0
Cell Periphery 1 5 2 2 0 0 0 1 3 1 1 1 0 2 1 1 1 4 4 1 2 1
Cytoplasm 348 512 239 165 107 275 300 293 291 268 194 195 282 283 294 218 308 430 155 257 241 318
Endoplasmic Reticulum 1 5 1 0 1 0 1 0 0 0 0 0 0 0 0 12 0 2 4 0 1 0
Endosome 1 0 2 0 0 1 0 1 1 0 0 1 0 0 0 2 5 6 7 1 2 3
Golgi 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 3 0 3 0
Mitochondria 7 9 2 1 7 15 15 16 92 68 73 71 1 0 0 6 17 23 41 8 15 9
Nucleus 1 0 0 0 1 1 1 6 1 1 0 4 1 1 0 0 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 1 0 1 0 1
Nucleolus 0 0 1 1 0 0 0 3 1 0 0 1 1 0 0 0 0 0 0 0 0 1
Peroxisomes 0 1 1 0 0 0 1 0 0 0 0 1 0 0 0 0 1 1 0 0 0 0
SpindlePole 1 3 1 1 0 4 6 5 2 3 1 4 0 0 0 1 3 7 2 1 2 2
Vac/Vac Membrane 2 1 1 0 1 0 3 1 1 1 0 3 0 0 0 4 7 2 3 2 2 2
Unique Cell Count 355 529 247 168 113 288 312 316 338 316 236 250 284 283 295 222 359 509 258 285 294 361
Labelled Cell Count 363 541 256 176 120 310 348 339 406 352 275 296 286 288 296 244 359 509 258 285 294 361


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.6 5.0 4.4 3.8 4.1 4.1 4.6 3.3 3.7 3.3 3.5 4.4 4.6 4.4 4.0 4.8 4.9 4.7 4.9 5.0
Std Deviation (1e-4) 0.9 0.7 1.9 2.2 1.7 1.7 1.7 2.3 1.6 1.0 1.0 0.9 1.0 1.0 1.5 1.1 1.1 1.2 1.3 1.4 1.2
Intensity Change (Log2) -0.16 -0.38 -0.27 -0.28 -0.11 -0.57 -0.45 -0.6 -0.53 -0.17 -0.12 -0.17 -0.32 -0.05 -0.02 -0.07 -0.01 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole