ORF
Human Ortholog
Description Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 0.96 0.93 0.82 0.72 0.59 0.5 0.44 0.39 0.99 0.99 0.98 0.95 0.93 0.97 0.94 0.93 0.89 0.89 0.91 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.17 0.08 0.38 0.37 0.46 0.48 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0.07 0.26 0.17 0.22 0.23 0.14 0 0 0 0 0.07 0 0 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 2 0 2 3
Bud 0 2 0 0 0 2 0 2 1 3 2 1 0 0 0 0 0 0 0 0 1 3 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 1 0 2 1 4 1 3 0 4 1 1 0 3 1 3 6 7 0 0 0 0 0 0
Cytoplasm 167 318 243 175 241 171 174 140 89 76 69 297 394 294 96 102 149 164 305 232 209 314 280
Endoplasmic Reticulum 0 2 0 0 1 2 1 4 1 0 6 0 0 0 3 3 2 0 1 2 1 1 3
Endosome 0 0 1 0 0 0 2 5 5 1 3 0 1 0 0 0 0 0 4 2 3 3 3
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 1 0 1 3 0 1 2 6
Mitochondria 0 3 0 4 3 36 20 91 65 79 84 0 0 0 2 1 2 0 1 1 3 4 4
Nucleus 0 1 4 3 1 6 4 4 3 7 12 3 2 4 2 1 3 1 2 1 1 0 1
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 2 0 0 0 0 0 0 1 0 1 1 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1
Vac/Vac Membrane 5 0 5 6 22 15 63 40 39 40 24 0 2 3 3 8 3 2 4 13 5 4 2
Unique Cell Count 170 323 249 182 260 208 240 238 178 173 176 300 398 299 101 110 153 174 329 262 234 346 318
Labelled Cell Count 173 326 255 189 272 233 268 287 209 208 204 302 402 303 110 122 166 174 329 262 234 346 318


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.3 8.9 8.8 8.1 9.1 6.0 7.3 6.3 6.6 5.8 6.2 10.0 9.9 10.9 13.1 14.4 12.5 8.1 8.9 9.1
Std Deviation (1e-4) 1.3 1.2 1.4 1.5 1.7 1.4 1.4 1.3 1.3 1.0 1.3 1.3 1.7 1.6 3.1 2.9 2.5 1.4 1.5 1.6
Intensity Change (Log2) -0.12 0.05 -0.55 -0.26 -0.47 -0.41 -0.59 -0.49 0.19 0.17 0.31 0.58 0.72 0.51 -0.11 0.03 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 -2.6 -5.6 -7.8 -10.4 -11.7 -12.6 -13.4 1.3 1.4 0.6 -1.2 -2.2 -0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.8 4.7 10.8 10.4 11.8 12.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 2.8 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.2 2.7 7.7 5.6 6.7 6.9 4.7 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4231 3.6685 3.0487 2.7997 2.1644 3.0127 4.3907 4.8814 4.5068 4.0466 3.5423 4.2058 7.7695 9.2741 8.0364 8.211 7.9423 8.046
Actin 0.0314 0.0011 0.0041 0.0087 0.0128 0.0144 0.0724 0.012 0.024 0.0011 0.0082 0.0014 0.0144 0.0008 0.0057 0.0049 0.0065 0.0023
Bud 0.0059 0.0008 0.0003 0.0005 0.0004 0.0008 0.0011 0.0002 0.0029 0.0002 0.0008 0.0001 0.0007 0.0002 0.0002 0.0001 0.0001 0.0008
Bud Neck 0.0079 0.0002 0.0004 0.0008 0.0009 0.0017 0.0031 0.0002 0.0141 0.0004 0.0014 0.001 0.0012 0.0001 0.0003 0.0005 0.0003 0.0022
Bud Periphery 0.0016 0.0001 0.0001 0.0008 0.0004 0.0006 0.0013 0.0001 0.0035 0.0001 0.0006 0 0.0007 0 0.0001 0 0 0.0008
Bud Site 0.0032 0.0077 0.0004 0.0008 0.0014 0.0002 0.0073 0.0012 0.0124 0.0016 0.0014 0 0.0061 0.0006 0.0003 0.0002 0.0003 0.0002
Cell Periphery 0.0003 0.0001 0 0.0001 0.0001 0 0.0003 0.0001 0.0008 0 0.0001 0 0.0003 0 0.0001 0 0.0001 0
Cytoplasm 0.8035 0.9564 0.9343 0.9131 0.8218 0.9186 0.6461 0.9283 0.8366 0.8354 0.8689 0.9633 0.8429 0.9775 0.9192 0.8159 0.9399 0.9433
Cytoplasmic Foci 0.0261 0.0067 0.0087 0.0083 0.0203 0.0172 0.0772 0.0082 0.0232 0.0812 0.0351 0.0082 0.0088 0.0063 0.0155 0.0189 0.0064 0.009
Eisosomes 0.0005 0 0 0.0002 0.0001 0.0001 0.0008 0 0.0001 0 0 0 0.0002 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0083 0.0016 0.0049 0.0034 0.0052 0.0031 0.006 0.0055 0.0084 0.0034 0.0082 0.0035 0.0078 0.0011 0.0025 0.0008 0.0077 0.003
Endosome 0.0353 0.0037 0.0078 0.0236 0.0446 0.0137 0.0524 0.0025 0.0154 0.0503 0.0171 0.0031 0.0143 0.0027 0.0095 0.0064 0.0037 0.0061
Golgi 0.006 0.0003 0.001 0.0027 0.0114 0.0017 0.0124 0.0012 0.0119 0.0063 0.0339 0.0002 0.0025 0.0004 0.0016 0.0019 0.0006 0.0005
Lipid Particles 0.0062 0 0.0025 0.001 0.008 0.0009 0.0134 0.0001 0.0095 0.0012 0.0009 0.0002 0.0087 0.0001 0.0019 0.0147 0.0003 0.0001
Mitochondria 0.003 0.0003 0.0062 0.0106 0.0081 0.0009 0.0225 0.0006 0.0088 0.0012 0.0054 0.0001 0.0025 0.0001 0.0018 0.0005 0.0002 0.0006
None 0.0254 0.0149 0.0171 0.0074 0.0223 0.0058 0.0177 0.0332 0.0122 0.0067 0.0022 0.0107 0.0443 0.0074 0.0261 0.0903 0.03 0.0244
Nuclear Periphery 0.0095 0.0008 0.0022 0.0053 0.0064 0.0028 0.0271 0.0008 0.0019 0.0009 0.0025 0.0012 0.0165 0.0004 0.0014 0.0024 0.001 0.0007
Nucleolus 0.003 0.0001 0.0002 0.0002 0.0018 0.0001 0.0014 0 0.0005 0.0001 0 0 0.0003 0 0.0001 0.001 0 0
Nucleus 0.009 0.0028 0.005 0.0071 0.0077 0.0116 0.0076 0.0021 0.0025 0.0014 0.0037 0.0046 0.0165 0.0015 0.0033 0.0022 0.0017 0.0042
Peroxisomes 0.0024 0.0002 0.0011 0.0003 0.003 0.0006 0.0122 0.0026 0.0074 0.0055 0.0083 0.0001 0.0067 0.0002 0.0052 0.0071 0.0001 0.0008
Punctate Nuclear 0.0064 0.0007 0.0017 0.0006 0.0197 0.0032 0.0115 0.0007 0.0009 0.0018 0.0004 0.001 0.0022 0.0003 0.0044 0.0314 0.0004 0.0004
Vacuole 0.0038 0.0011 0.0015 0.0028 0.0025 0.0015 0.0034 0.0004 0.0023 0.001 0.0007 0.0011 0.0018 0.0002 0.0005 0.0003 0.0005 0.0005
Vacuole Periphery 0.0012 0.0002 0.0004 0.0017 0.0009 0.0005 0.0028 0.0001 0.0006 0.0003 0.0003 0.0001 0.0008 0.0001 0.0002 0.0002 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.07 28.5253 23.3951 27.2347 35.9286 30.2135 21.6315 24.0768 21.1248 31.6974
Translational Efficiency 1.6954 1.2233 1.3296 1.197 1.1362 2.1697 1.8504 1.5408 1.9916 1.4936

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
169 1219 154 1455 1965 2025 1879 78 2134 3244 2033 1533

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 942.86 869.72 1288.51 1070.01 912.59 995.08 1144.92 1334.94 914.99 947.97 1155.80 1083.49
Standard Deviation 150.55 137.65 155.27 161.44 121.24 136.41 163.20 248.72 124.09 149.74 167.00 176.84
Intensity Change Log 2 -0.116493 0.450588 0.182509 0.124846 0.327208 0.548736 0.007252 0.391222 0.374238

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.004673 0.000350 0.000871 0.000870 0.000194 0.000445 0.001084 0.002859 0.000548 0.000409 0.001067 0.000972
Bud Neck 0.012503 0.004342 0.004652 0.007752 0.003303 0.006352 0.001769 0.021496 0.004031 0.005597 0.001987 0.008451
Bud Site 0.149370* 0.022130 0.009403 0.040733 0.006284 0.016394 0.010898 0.101178* 0.017616 0.018549 0.010785 0.043809
Cell Periphery 0.000568 0.000093 0.000277 0.000102 0.000072 0.000065 0.000068 0.000155 0.000111 0.000076 0.000084 0.000105
Cytoplasm 0.243119* 0.733753* 0.408627* 0.566941* 0.728100* 0.750219* 0.761723* 0.432468* 0.689692* 0.744031* 0.734976* 0.560099*
Cytoplasmic Foci 0.355601* 0.096202 0.020410 0.061625 0.107749 0.087121 0.019329 0.132245 0.127377 0.090533 0.019411 0.065219
Eisosomes 0.000162 0.000017 0.000052 0.000024 0.000022 0.000020 0.000010 0.000030 0.000033 0.000019 0.000013 0.000024
Endoplasmic Reticulum 0.009749 0.001201 0.004110 0.001745 0.001549 0.000841 0.000851 0.000508 0.002198 0.000977 0.001098 0.001682
Endosome 0.025898 0.009847 0.007745 0.018940 0.005592 0.005705 0.004538 0.032184 0.007200 0.007261 0.004781 0.019614
Golgi 0.039148 0.002771 0.000402 0.003991 0.001847 0.002414 0.001606 0.003135 0.004801 0.002548 0.001514 0.003948
Lipid Particles 0.008230 0.001017 0.002328 0.002430 0.001592 0.000619 0.000948 0.001994 0.002117 0.000769 0.001053 0.002408
Mitochondria 0.030867 0.001043 0.000312 0.003764 0.000734 0.002466 0.000462 0.000124 0.003121 0.001931 0.000451 0.003579
Mitotic Spindle 0.016096 0.003884 0.004588 0.025148 0.003305 0.006029 0.016261 0.016606 0.004318 0.005223 0.015377 0.024714
None 0.000598 0.006581 0.002783 0.004934 0.003790 0.004576 0.004898 0.000907 0.003537 0.005330 0.004738 0.004729
Nuclear Periphery 0.000562 0.000137 0.001778 0.000394 0.000206 0.000106 0.000374 0.000156 0.000234 0.000118 0.000480 0.000382
Nuclear Periphery Foci 0.003937 0.002277 0.012226 0.001586 0.001445 0.000723 0.002598 0.001044 0.001643 0.001307 0.003327 0.001559
Nucleolus 0.001260 0.001208 0.000253 0.001265 0.000350 0.000601 0.000217 0.000245 0.000422 0.000829 0.000219 0.001213
Nucleus 0.024022 0.049239 0.238777* 0.043833 0.081513 0.062109 0.107695 0.016386 0.076960 0.057273 0.117624 0.042437
Peroxisomes 0.019016 0.003238 0.000284 0.003422 0.001412 0.002740 0.000791 0.012696 0.002806 0.002927 0.000753 0.003894
Vacuole 0.043022 0.057882 0.279763* 0.205886* 0.050030 0.049560 0.062946 0.221958* 0.049475 0.052688 0.079370 0.206704*
Vacuole Periphery 0.011601 0.002788 0.000359 0.004613 0.000912 0.000893 0.000936 0.001625 0.001759 0.001605 0.000892 0.004461

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 7.47 6.40 6.90 -3.77 2.46 -2.92 -2.55 -3.58 -3.22 -2.77 1.73 -0.88 -2.24 -3.90 -0.31
Bud Neck 4.25 3.87 2.97 -3.68 -2.48 -4.04 3.14 -2.65 -1.97 -2.95 -2.54 4.33 -4.82 -2.45 -9.47
Bud Site 9.19 10.11 8.87 -1.68 -7.75 -7.16 -1.91 -5.20 -4.55 -5.06 -0.35 5.36 -4.68 -5.02 -9.87
Cell Periphery 6.21 3.80 6.46 1.86 9.60 1.54 0.62 -4.76 -5.02 -4.87 4.36 3.27 3.38 -1.17 0.37
Cytoplasm -25.51 -3.06 -15.23 18.44 -11.61 -2.96 4.73 9.42 10.05 8.39 -7.50 3.15 17.08 24.98 14.60
Cytoplasmic Foci 13.87 18.71 16.69 10.50 -13.77 4.70 25.34 0.16 -1.26 -6.12 8.43 28.52 17.59 12.31 -13.92
Eisosomes 9.41 6.93 9.11 -3.18 8.48 1.66 8.42 -3.58 -4.12 -5.89 7.46 9.71 5.10 -3.52 -7.26
Endoplasmic Reticulum 4.78 2.98 4.82 0.39 6.23 1.88 3.71 2.81 0.19 0.07 3.92 4.71 3.84 -0.48 -0.32
Endosome 5.81 7.42 5.70 -0.22 -3.56 -0.06 5.12 -1.65 -1.64 -2.20 0.07 7.44 -2.84 -2.80 -7.13
Golgi 6.35 6.76 6.50 1.92 -4.49 -0.97 2.51 0.54 1.19 -0.84 3.50 6.39 4.47 1.70 -2.87
Lipid Particles 3.75 3.00 3.20 -1.85 1.13 5.96 2.62 -2.09 -2.80 -2.47 6.17 3.91 0.14 -2.78 -1.84
Mitochondria 4.02 4.10 3.91 -1.25 -2.59 -2.52 2.44 3.82 3.52 3.12 1.62 4.41 1.81 0.35 -2.64
Mitotic Spindle 2.48 0.90 -1.08 -6.29 -2.15 -2.01 -5.17 -2.05 -1.57 -0.38 -0.91 -4.71 -6.57 -6.20 -2.74
None -4.73 -4.84 -3.89 2.36 -1.00 -0.83 -1.17 6.09 4.86 11.49 -2.17 -1.48 0.65 2.45 1.87
Nuclear Periphery 1.59 -5.95 -0.30 -12.24 9.84 5.32 -11.58 -1.93 -5.05 6.96 4.20 -11.94 -8.77 -13.64 1.77
Nuclear Periphery Foci 0.85 -2.61 1.19 2.04 8.77 5.28 -5.91 -1.80 -3.70 1.82 1.30 -6.20 -0.65 -2.61 6.58
Nucleolus 0.19 1.53 0.59 0.86 -4.55 -1.44 3.62 2.49 1.98 0.40 -2.39 3.00 -3.23 -0.10 -4.90
Nucleus -4.53 -13.02 -6.33 -2.78 11.45 6.64 -14.08 10.28 6.83 18.51 7.61 -17.53 7.22 0.34 23.32
Peroxisomes 5.66 6.78 5.96 1.58 -9.96 -2.95 3.78 -3.34 -2.67 -3.77 -0.27 7.09 0.06 0.37 -8.93
Vacuole -2.89 -14.42 -26.18 -29.32 3.99 -0.12 -14.82 -8.65 -8.64 -6.37 -1.85 -18.26 -33.55 -33.29 -18.10
Vacuole Periphery 3.81 4.86 4.05 1.17 -4.43 0.21 2.49 0.65 0.53 -0.80 0.78 5.16 -0.58 -1.13 -3.89