Standard name
Human Ortholog
Description Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.05 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.95 0.97 0.87 0.94 0.91 0.93 0.85 0.71 0.76 0.67 0.98 0.96 0.97 0.95 0.95 0.93 0.95 0.93 0.93 0.77 0.84 0.84
Endoplasmic Reticulum 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0.16 0.1 0.08
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.08 0.05 0.2 0.38 0.4 0.35 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 1 0 2 2 0 0 0 0 0 0 0 0 1 1 0 0 0 1 1 0
Bud 0 0 0 0 2 1 4 2 5 6 2 7 0 0 0 0 0 0 2 7 3 0 6 9
Bud Neck 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 2 3 16 11 15 2 7 4 4 4 5 2 4 21 15 7 20 15 0 0 1 0 0 1
Cytoplasm 149 398 281 146 172 163 212 148 177 137 124 132 375 516 489 230 210 211 139 387 282 173 429 421
Endoplasmic Reticulum 3 6 15 3 9 0 1 0 0 0 0 1 9 17 11 11 9 15 0 3 4 36 49 42
Endosome 0 0 0 0 0 1 2 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0 2 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 2 2
Mitochondria 0 0 0 0 3 5 18 8 41 73 66 69 0 0 0 1 1 0 0 2 2 1 2 3
Nucleus 0 2 0 0 1 0 0 3 2 4 3 8 1 1 2 0 0 1 0 3 1 0 0 2
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1
Nucleolus 0 0 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 3 3 1 7 7 0 5 8 6 0 6 2 2 6 7 6 11 0 5 3 5 9 11
Unique Cell Count 151 405 297 150 198 173 232 159 209 193 163 196 384 535 505 243 221 227 147 416 303 226 512 503
Labelled Cell Count 154 412 316 161 210 179 248 172 239 231 201 227 391 558 526 257 247 254 147 416 303 226 512 503


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 53.2 49.4 33.2 33.6 16.1 8.0 6.2 5.8 4.9 4.7 4.6 4.9 54.7 46.3 35.6 42.3 44.7 44.2 42.4 34.3 32.7
Std Deviation (1e-4) 13.8 14.2 8.4 8.3 4.6 2.7 1.7 1.2 1.4 1.2 1.1 1.5 16.3 14.1 12.2 17.1 18.1 17.4 13.9 10.9 10.7
Intensity Change (Log2) 0.02 -1.04 -2.06 -2.42 -2.51 -2.75 -2.81 -2.85 -2.75 0.72 0.48 0.1 0.35 0.43 0.41 0.35 0.05 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.8 1.0 -2.3 -1.3 -1.4 -2.0 0 0 -2.5 -3.3 -1.0 -1.7 -1.4 1.6 0.6
Cytoplasm 1.3 -3.0 -0.2 -1.5 -0.7 -3.8 -7.2 -5.9 -8.0 2.1 1.3 1.6 0 0.2 -0.8
Endoplasmic Reticulum -1.5 -0.3 -3.0 -3.1 -2.9 -3.3 0 0 -2.8 -1.9 -1.3 -2.2 -0.3 -0.5 0.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.9 0 8.0 0 0 11.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 54.2569 75.1765 67.2456 53.8748 49.4396 72.8518 54.2584 76.1811 67.8019 63.2645 49.2742 70.1253 49.416 76.6626 68.6327 53.8299 72.1491 63.9575
Actin 0.0227 0.0008 0.0005 0.0181 0.013 0.0011 0.0147 0.0031 0.002 0.0062 0.0024 0.0016 0.0137 0.0015 0.0008 0.0001 0.0099 0.0052
Bud 0.0022 0.0013 0.0014 0.0017 0.001 0.0031 0.0023 0.0033 0.0053 0.0008 0.0025 0.0059 0.0019 0.0027 0.0039 0.002 0.0005 0.0017
Bud Neck 0.0012 0.0005 0.0008 0.0006 0.001 0.0026 0.0043 0.0004 0.0006 0.0005 0.0009 0.0016 0.0013 0.0002 0.0005 0.0004 0.0006 0.0009
Bud Periphery 0.0038 0.0014 0.0032 0.0015 0.0009 0.0037 0.004 0.0013 0.0031 0.0007 0.0028 0.0067 0.0037 0.0017 0.0039 0.0018 0.0023 0.0044
Bud Site 0.0037 0.0027 0.0005 0.0007 0.0008 0.0012 0.0036 0.0016 0.0033 0.0003 0.0008 0.0004 0.0051 0.0021 0.0014 0.0001 0.0004 0.0005
Cell Periphery 0.009 0.0114 0.0085 0.0015 0.0021 0.0078 0.0081 0.0065 0.0051 0.0091 0.0045 0.0079 0.0222 0.0203 0.0295 0.0261 0.053 0.0272
Cytoplasm 0.6527 0.9093 0.8248 0.7201 0.6387 0.8043 0.7695 0.9147 0.8793 0.7441 0.699 0.8472 0.6535 0.8668 0.7993 0.8418 0.7512 0.7662
Cytoplasmic Foci 0.0172 0.0013 0.002 0.0119 0.0218 0.0105 0.0115 0.0017 0.0032 0.0065 0.0365 0.0063 0.0165 0.0012 0.0051 0.0016 0.0224 0.0066
Eisosomes 0.001 0 0 0.0003 0.0002 0.0001 0.0004 0.0001 0.0001 0.0001 0.0003 0 0.0006 0 0 0 0 0.0003
Endoplasmic Reticulum 0.0543 0.0157 0.0285 0.0063 0.0061 0.0478 0.0405 0.0196 0.0193 0.0026 0.0198 0.0631 0.0436 0.0065 0.0107 0.0017 0.0023 0.0336
Endosome 0.0121 0.0033 0.0058 0.0165 0.0197 0.0213 0.0133 0.0019 0.0056 0.0175 0.0409 0.0132 0.0228 0.0015 0.0049 0.0057 0.011 0.0132
Golgi 0.0031 0.0014 0.0004 0.0041 0.0213 0.0039 0.0039 0.0006 0.0013 0.0093 0.0077 0.0065 0.0044 0.0002 0.0011 0.0002 0.0046 0.0044
Lipid Particles 0.0091 0.0008 0.0012 0.0039 0.0199 0.0014 0.0067 0.0007 0.005 0.0086 0.0613 0.0023 0.0086 0.0008 0.0027 0.0001 0.0124 0.0183
Mitochondria 0.022 0.0007 0.0087 0.049 0.0521 0.0099 0.0081 0.0007 0.0063 0.029 0.0244 0.0019 0.0295 0.003 0.009 0.0016 0.0006 0.0476
None 0.0059 0.0007 0.0005 0.0049 0.0064 0.0045 0.0044 0.007 0.0019 0.0008 0.0175 0.0011 0.0096 0.0003 0.0007 0.0003 0.0007 0.0012
Nuclear Periphery 0.0216 0.004 0.0115 0.009 0.0061 0.0045 0.0133 0.0032 0.0039 0.0034 0.0095 0.0046 0.0145 0.0015 0.0022 0.0022 0.0015 0.0053
Nucleolus 0.0077 0.0004 0.0009 0.0014 0.007 0.0048 0.0053 0.0003 0.0011 0.0002 0.0031 0.0002 0.0059 0.0007 0.0008 0.0003 0.0004 0.0083
Nucleus 0.0082 0.0022 0.0048 0.0069 0.0138 0.0072 0.0102 0.0026 0.0028 0.0021 0.0052 0.0022 0.008 0.0012 0.0025 0.0026 0.0012 0.0037
Peroxisomes 0.0051 0 0.0006 0.0017 0.0641 0.0005 0.0039 0.0001 0.0003 0.0113 0.0033 0.0001 0.003 0 0.0006 0 0.0164 0.0022
Punctate Nuclear 0.0085 0.0001 0.0001 0.0012 0.0013 0.0003 0.0067 0.0002 0.0002 0.0009 0.0011 0.0006 0.0027 0 0.0003 0 0.0051 0.0009
Vacuole 0.1059 0.0373 0.0791 0.1053 0.0812 0.0453 0.0534 0.0281 0.0435 0.1286 0.0418 0.0209 0.0929 0.067 0.0981 0.0894 0.0858 0.0352
Vacuole Periphery 0.0232 0.0045 0.0161 0.0332 0.0214 0.0143 0.0118 0.0023 0.0066 0.0174 0.0147 0.0057 0.0359 0.0207 0.0218 0.0218 0.0176 0.013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4169.0712 4410.0259 4628.634 3694.311 3923.5767 3962.9056 3875.4257 3368.2938 3740.0597 3181.6917
Translational Efficiency 0.4671 0.486 0.5008 0.5834 0.5072 0.435 0.5001 0.5499 0.4593 0.5035

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1824 1470 542 11 1727 1932 1754 18 3551 3402 2296 29

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2900.47 3620.09 7005.98 321.24 2797.37 2395.96 6807.66 7090.99 2850.33 2924.91 6854.48 4523.15
Standard Deviation 754.90 974.21 1511.42 3.44 705.78 667.27 1507.78 2947.86 733.24 1015.22 1510.99 4022.88
Intensity Change Log 2 0.319739 1.272300 -3.174563 -0.223467 1.283088 1.341916 0.078401 1.277607 0.379493

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000078 0.000107 0.000192 0.000446 0.000088 0.000154 0.000090 0.000514 0.000083 0.000134 0.000114 0.000488
Bud Neck 0.000284 0.000558 0.006757 0.000006 0.000449 0.000321 0.001932 0.004630 0.000364 0.000423 0.003071 0.002876
Bud Site 0.005632 0.003892 0.006446 0.000045 0.004838 0.007588 0.003817 0.003855 0.005246 0.005991 0.004438 0.002410
Cell Periphery 0.003980 0.004602 0.041816 0.000306 0.003885 0.001586 0.004139 0.006198 0.003934 0.002889 0.013034 0.003963
Cytoplasm 0.893039* 0.814412* 0.427440* 0.000032 0.835042* 0.852558* 0.766649* 0.406544* 0.864833* 0.836075* 0.686575* 0.252350*
Cytoplasmic Foci 0.009794 0.010046 0.018032 0.000026 0.016044 0.027135 0.005678 0.022890 0.012834 0.019751 0.008594 0.014218
Eisosomes 0.000001 0.000001 0.000008 0.000826 0.000001 0.000002 0.000002 0.000023 0.000001 0.000002 0.000003 0.000327
Endoplasmic Reticulum 0.000814 0.000775 0.000450 0.000001 0.001404 0.001265 0.000205 0.000617 0.001101 0.001053 0.000263 0.000383
Endosome 0.000813 0.001073 0.000977 0.000000 0.001447 0.002645 0.000572 0.009721 0.001122 0.001966 0.000668 0.006033
Golgi 0.029328 0.049707 0.035676 0.000002 0.046822 0.045461 0.009707 0.015343 0.037836 0.047296 0.015837 0.009524
Lipid Particles 0.004695 0.006793 0.025109 0.000018 0.006081 0.002966 0.006738 0.016977 0.005369 0.004620 0.011075 0.010544
Mitochondria 0.000170 0.000171 0.000220 0.000011 0.000158 0.000686 0.000224 0.113844* 0.000164 0.000463 0.000223 0.070666
Mitotic Spindle 0.000768 0.002376 0.002626 0.000171 0.001892 0.004493 0.001897 0.028972 0.001315 0.003578 0.002069 0.018047
None 0.002006 0.002386 0.001491 0.997811* 0.001477 0.001922 0.002315 0.000513 0.001749 0.002123 0.002120 0.378799*
Nuclear Periphery 0.000095 0.000477 0.003695 0.000000 0.000215 0.000104 0.000637 0.000677 0.000153 0.000265 0.001359 0.000421
Nuclear Periphery Foci 0.004626 0.013998 0.017898 0.000001 0.009720 0.006673 0.006528 0.004195 0.007103 0.009838 0.009212 0.002604
Nucleolus 0.000065 0.000065 0.000174 0.000001 0.000035 0.000066 0.000176 0.002285 0.000050 0.000065 0.000176 0.001419
Nucleus 0.004129 0.009359 0.026491 0.000007 0.005206 0.004693 0.030398 0.013350 0.004653 0.006709 0.029475 0.008288
Peroxisomes 0.000028 0.000016 0.000019 0.000289 0.000023 0.000059 0.000019 0.001965 0.000025 0.000041 0.000019 0.001330
Vacuole 0.015666 0.037679 0.316825* 0.000000 0.025689 0.014335 0.136677 0.314325* 0.020541 0.024422 0.179203 0.195098
Vacuole Periphery 0.023992 0.041509 0.067655 0.000000 0.039483 0.025288 0.021598 0.032563 0.031526 0.032297 0.032471 0.020211

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin NaN NaN NaN NaN NaN -1.31 -2.24 -1.91 -1.52 -1.72 -1.77 -4.88 -2.06 -1.74 -1.68
Bud Neck NaN NaN NaN NaN NaN 2.88 -9.11 -1.87 -1.90 -1.32 -1.70 -13.59 -1.79 -1.77 -0.47
Bud Site NaN NaN NaN NaN NaN -3.39 0.09 -0.19 1.37 -0.22 -1.30 -0.34 1.67 2.37 1.87
Cell Periphery NaN NaN NaN NaN NaN 6.65 -0.42 -1.59 -3.22 -1.44 3.42 -9.32 -0.06 -1.04 6.07
Cytoplasm NaN NaN NaN NaN NaN -2.23 12.49 5.90 6.14 4.40 5.18 27.57 10.74 10.23 6.94
Cytoplasmic Foci NaN NaN NaN NaN NaN -7.15 8.54 -0.94 -0.22 -1.49 -7.29 1.40 -0.62 0.09 -0.75
Eisosomes NaN NaN NaN NaN NaN -1.23 -5.15 -1.76 -1.66 -1.62 -1.62 -11.22 -2.32 -2.32 -2.30
Endoplasmic Reticulum NaN NaN NaN NaN NaN 0.54 6.89 2.86 2.44 -0.71 0.29 7.73 3.91 3.65 -0.03
Endosome NaN NaN NaN NaN NaN -4.25 6.91 -1.36 -1.03 -1.56 -4.88 4.43 -1.23 -0.87 -1.37
Golgi NaN NaN NaN NaN NaN 0.45 15.99 7.97 7.60 -1.36 -4.27 14.70 10.18 12.97 2.45
Lipid Particles NaN NaN NaN NaN NaN 4.60 -1.48 -1.93 -2.43 -1.74 1.36 -8.62 -1.42 -1.60 0.08
Mitochondria NaN NaN NaN NaN NaN -1.46 -2.68 -1.37 -1.36 -1.37 -1.44 -2.54 -1.35 -1.35 -1.35
Mitotic Spindle NaN NaN NaN NaN NaN -3.56 -1.70 -1.09 -0.93 -1.01 -5.02 -3.23 -1.08 -0.85 -0.89
None NaN NaN NaN NaN NaN -0.82 -1.39 6.18 2.65 3.39 -1.06 -0.68 -4.12 -4.12 -4.12
Nuclear Periphery NaN NaN NaN NaN NaN 5.05 -6.24 -1.29 -1.37 -0.80 -3.77 -9.59 -1.25 -1.12 0.50
Nuclear Periphery Foci NaN NaN NaN NaN NaN 3.22 2.55 0.07 -0.51 -0.38 -4.22 -4.17 0.38 1.19 1.20
Nucleolus NaN NaN NaN NaN NaN -1.01 -12.65 -1.06 -1.05 -1.04 -0.70 -9.84 -1.05 -1.05 -1.02
Nucleus NaN NaN NaN NaN NaN 0.63 -18.70 -2.24 -2.30 3.31 -3.91 -21.65 -1.76 -1.27 6.06
Peroxisomes NaN NaN NaN NaN NaN -2.72 0.21 -1.58 -1.55 -1.58 -1.95 0.79 -1.71 -1.69 -1.71
Vacuole NaN NaN NaN NaN NaN 5.70 -23.74 -5.04 -5.21 -3.01 -2.68 -33.65 -3.88 -3.80 -0.50
Vacuole Periphery NaN NaN NaN NaN NaN 4.86 7.11 1.20 0.02 -0.46 -0.36 1.20 2.07 2.17 1.75