Standard name
Description Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.13 0.14 0.16 0.18 0.13 0.11 0.12 0.11 0.05 0.08 0.09 0.23 0.05 0.08 0.17 0.11 0 0.05 0.06 0.06 0.11 0.17 0.09
Bud 0.06 0.05 0.05 0.06 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0.06 0.15 0.19 0.18 0.17 0.14 0.16
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.12 0.08 0.08 0.12 0.07 0.10 0.07 0.06 0.05 0.08 0.20 0.20 0.18 0.38 0.39 0.36 0 0 0 0 0.08 0.08
Cytoplasm 0.33 0.22 0.19 0.26 0.23 0.22 0.27 0.23 0.29 0.38 0.28 0.18 0.31 0.34 0.30 0.40 0.34 0 0 0 0 0 0
Endoplasmic Reticulum 0.60 0.60 0.58 0.53 0.60 0.59 0.56 0.52 0.40 0.32 0.33 0.66 0.59 0.52 0.05 0.05 0 0.64 0.56 0.58 0.52 0.47 0.50
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0.19 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.05 0 0.14 0.11 0.12 0 0 0 0.10 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.07 0.12 0.10 0.06 0.15 0.12 0.24 0.21 0.14 0.21 0 0 0 0.12 0.06 0.08 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 26 29 33 34 28 28 37 38 10 15 17 54 10 20 7 12 3 10 15 15 24 57 25
Bud 12 11 10 12 5 9 12 13 4 8 12 13 4 5 1 3 6 34 46 45 38 46 49
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 5 7 10 18 9
Bud Site 0 0 7 1 0 1 0 0 1 1 0 4 0 0 0 0 0
Cell Periphery 8 25 17 15 27 19 30 23 13 9 16 46 42 44 15 45 35 2 4 2 9 25 24
Cytoplasm 67 45 38 49 51 58 84 81 63 74 55 41 64 84 12 46 33 6 6 6 3 4 6
Endoplasmic Reticulum 123 125 118 101 132 151 175 180 86 63 64 152 121 129 2 6 4 150 134 148 115 157 148
Endosome 3 0 0 3 1 1 3 0 0 0 3 1 5 2 0 0 0 1 1 0 1 0 1
Golgi 1 2 0 2 2 0 0 2 1 0 1 0 2 0 2 13 18 3 1 0 1 3 0
Mitochondria 5 5 0 1 3 12 15 10 30 22 23 3 2 0 4 2 0 7 5 8 7 9 9
Nucleus 0 0 2 1 0 2 1 2 0 0 2 0 0 0 1 1 0 0 0 0 0 1 0
Nuclear Periphery 0 1 0 2 1 0 6 1 5 6 9 1 0 0 0 0 0 5 3 6 1 0 4
Nucleolus 0 1 0 0 0 0 0 0 0 1 0 1 0 0 0 1 0 0 0 0 0 0 1
Peroxisomes 0 2 0 0 0 0 0 0 0 0 0 1 1 2 0 0 0 0 0 0 0 0 1
SpindlePole 1 4 8 3 0 0 0 0 0 0 0 2 4 4 0 0 0 0 0 1 0 0 0
Vac/Vac Membrane 6 15 25 19 14 39 36 85 45 28 41 0 6 8 5 7 8 7 13 10 5 6 10
Unique Cell Count 204 207 204 190 219 258 310 348 217 194 196 232 206 250 40 114 97 236 240 256 221 335 300
Labelled Cell Count 252 265 258 243 264 320 399 435 258 227 243 319 261 298 49 136 107 236 240 256 221 335 300

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Endoplasmic Reticulum

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.8 14.8 13.7 13.1 13.1 11.6 11.7 12.1 10.5 9.8 9.9 14.7 13.8 12.8 31.7 32.2 32.5 11.9 11.4 11.1
Std Deviation (1e-4) 7.2 7.1 5.9 5.7 4.8 3.8 3.9 3.7 3.9 3.4 3.5 7.5 6.6 6.1 18.9 18.2 18.1 6.2 6.0 5.2
Intensity Change (Log2) -0.07 -0.07 -0.24 -0.23 -0.18 -0.38 -0.48 -0.46 0.11 0.02 -0.1 1.21 1.24 1.25 -0.2 -0.26 -0.3

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.5 -1.0 -1.7 -1.4 -1.8 -3.9 -2.6 -2.3 1.9 -3.7 -2.7 0.2 -1.4 -3.3
Bud 0.6 -1.5 -0.8 -0.6 -0.7 -1.7 -0.4 0.5 0.3 -1.7 -1.7 0 0 0.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 -1.1 0 0 0 0 0
Cell Periphery -0.2 1.3 -0.4 0.5 -0.8 -0.9 -1.5 -0.1 3.4 3.5 2.9 5.0 6.7 6.0
Cytoplasm 1.2 1.3 0.8 2.4 1.7 2.1 3.1 1.5 -1.8 0.1 0.5 2.6 4.0 3.3
Endoplasmic Reticulum -0.9 0.5 0.1 -0.3 -1.4 -3.7 -5.1 -5.1 1.6 0.2 -1.3 -6.1 -9.2 -8.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 6.3
Mitochondria 0 0 3.1 3.2 0 5.5 4.9 5.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -1.4 0 0 0 0 0 0 0 0 -1.2 -1.5 0 0 0
Vacuole -0.8 -1.8 -0.2 -1.6 1.3 0.3 -1.5 -2.5 -4.5 -3.5 -3.7 0 -2.8 -2.1