Standard name
Human Ortholog
Description Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.13 0.14 0.16 0.18 0.13 0.11 0.12 0.11 0.05 0.08 0.09 0.23 0.05 0.08 0.17 0.11 0 0.05 0.06 0.06 0.11 0.17 0.09
Bud 0.06 0.05 0.05 0.06 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0.06 0.15 0.19 0.18 0.17 0.14 0.16
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.12 0.08 0.08 0.12 0.07 0.1 0.07 0.06 0.05 0.08 0.2 0.2 0.18 0.38 0.39 0.36 0 0 0 0 0.08 0.08
Cytoplasm 0.33 0.22 0.19 0.26 0.23 0.22 0.27 0.23 0.29 0.38 0.28 0.18 0.31 0.34 0.3 0.4 0.34 0 0 0 0 0 0
Endoplasmic Reticulum 0.6 0.6 0.58 0.53 0.6 0.59 0.56 0.52 0.4 0.32 0.33 0.66 0.59 0.52 0.05 0.05 0 0.64 0.56 0.58 0.52 0.47 0.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0.19 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.05 0 0.14 0.11 0.12 0 0 0 0.1 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.07 0.12 0.1 0.06 0.15 0.12 0.24 0.21 0.14 0.21 0 0 0 0.12 0.06 0.08 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 26 29 33 34 28 28 37 38 10 15 17 54 10 20 7 12 3 10 15 15 24 57 25
Bud 12 11 10 12 5 9 12 13 4 8 12 13 4 5 1 3 6 34 46 45 38 46 49
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 5 7 10 18 9
Bud Site 0 0 7 1 0 1 0 0 1 1 0 4 0 0 0 0 0
Cell Periphery 8 25 17 15 27 19 30 23 13 9 16 46 42 44 15 45 35 2 4 2 9 25 24
Cytoplasm 67 45 38 49 51 58 84 81 63 74 55 41 64 84 12 46 33 6 6 6 3 4 6
Endoplasmic Reticulum 123 125 118 101 132 151 175 180 86 63 64 152 121 129 2 6 4 150 134 148 115 157 148
Endosome 3 0 0 3 1 1 3 0 0 0 3 1 5 2 0 0 0 1 1 0 1 0 1
Golgi 1 2 0 2 2 0 0 2 1 0 1 0 2 0 2 13 18 3 1 0 1 3 0
Mitochondria 5 5 0 1 3 12 15 10 30 22 23 3 2 0 4 2 0 7 5 8 7 9 9
Nucleus 0 0 2 1 0 2 1 2 0 0 2 0 0 0 1 1 0 0 0 0 0 1 0
Nuclear Periphery 0 1 0 2 1 0 6 1 5 6 9 1 0 0 0 0 0 5 3 6 1 0 4
Nucleolus 0 1 0 0 0 0 0 0 0 1 0 1 0 0 0 1 0 0 0 0 0 0 1
Peroxisomes 0 2 0 0 0 0 0 0 0 0 0 1 1 2 0 0 0 0 0 0 0 0 1
SpindlePole 1 4 8 3 0 0 0 0 0 0 0 2 4 4 0 0 0 0 0 1 0 0 0
Vac/Vac Membrane 6 15 25 19 14 39 36 85 45 28 41 0 6 8 5 7 8 7 13 10 5 6 10
Unique Cell Count 204 207 204 190 219 258 310 348 217 194 196 232 206 250 40 114 97 236 240 256 221 335 300
Labelled Cell Count 252 265 258 243 264 320 399 435 258 227 243 319 261 298 49 136 107 236 240 256 221 335 300


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.8 14.8 13.7 13.1 13.1 11.6 11.7 12.1 10.5 9.8 9.9 14.7 13.8 12.8 31.7 32.2 32.5 11.9 11.4 11.1
Std Deviation (1e-4) 7.2 7.1 5.9 5.7 4.8 3.8 3.9 3.7 3.9 3.4 3.5 7.5 6.6 6.1 18.9 18.2 18.1 6.2 6.0 5.2
Intensity Change (Log2) -0.07 -0.07 -0.24 -0.23 -0.18 -0.38 -0.48 -0.46 0.11 0.02 -0.1 1.21 1.24 1.25 -0.2 -0.26 -0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.5 -1.0 -1.7 -1.4 -1.8 -3.9 -2.6 -2.3 1.9 -3.7 -2.7 0.2 -1.4 -3.3
Bud 0.6 -1.5 -0.8 -0.6 -0.7 -1.7 -0.4 0.5 0.3 -1.7 -1.7 0 0 0.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 -1.1 0 0 0 0 0
Cell Periphery -0.2 1.3 -0.4 0.5 -0.8 -0.9 -1.5 -0.1 3.4 3.5 2.9 5.0 6.7 6.0
Cytoplasm 1.7 1.2 1.0 2.2 1.3 2.5 4.3 2.2 -0.3 2.9 3.6 1.6 4.2 2.9
Endoplasmic Reticulum -0.9 0.5 0.1 -0.3 -1.4 -3.7 -5.1 -5.1 1.6 0.2 -1.3 -6.1 -9.2 -8.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 6.3
Mitochondria 0 0 3.1 3.2 0 5.5 4.9 5.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -1.4 0 0 0 0 0 0 0 0 -1.2 -1.5 0 0 0
Vacuole -0.7 -2.1 0.9 -0.2 3.5 2.3 0.6 2.3 -5.5 -3.6 -3.7 0 -1.7 -1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.3024 12.4913 12.9235 10.1121 11.2532 13.4323 22.4672 11.3045 12.2494 14.8218 10.54 13.2942 27.2562 11.8014 12.5058 13.3509 12.7587 14.0601
Actin 0.0331 0.0239 0.0833 0.0415 0.0169 0.0458 0.0453 0.0298 0.0621 0.0148 0.0266 0.0458 0.0315 0.0257 0.0314 0.0124 0.0295 0.0431
Bud 0.0012 0.008 0.0079 0.0031 0.0017 0.0029 0.001 0.0097 0.0081 0.0023 0.0071 0.0034 0.0019 0.0186 0.0119 0.0076 0.0236 0.0042
Bud Neck 0.004 0.0037 0.0083 0.0301 0.0278 0.0267 0.0028 0.0056 0.0096 0.0029 0.0076 0.0203 0.0064 0.0026 0.0059 0.002 0.009 0.0358
Bud Periphery 0.0062 0.0179 0.0439 0.0274 0.0139 0.0356 0.0063 0.0446 0.035 0.0305 0.0451 0.0274 0.0184 0.063 0.055 0.0536 0.0857 0.0439
Bud Site 0.0046 0.1366 0.0436 0.0033 0.0033 0.002 0.0064 0.0656 0.0209 0.0006 0.0102 0.0013 0.0047 0.0665 0.0245 0.0015 0.0048 0.0016
Cell Periphery 0.0385 0.0621 0.0414 0.0216 0.0415 0.0288 0.0387 0.1002 0.0534 0.0396 0.0663 0.0325 0.0416 0.0931 0.0714 0.0449 0.0286 0.0505
Cytoplasm 0.0267 0.0768 0.018 0.0379 0.0497 0.0459 0.019 0.068 0.0379 0.0209 0.027 0.0462 0.0242 0.1008 0.0424 0.0228 0.038 0.0459
Cytoplasmic Foci 0.0117 0.0058 0.0026 0.004 0.0151 0.0038 0.0214 0.0041 0.0059 0.0006 0.0049 0.0015 0.0063 0.0022 0.002 0.0019 0.0024 0.0032
Eisosomes 0.009 0.001 0.0024 0.0008 0.0008 0.0012 0.0063 0.0051 0.0022 0.0005 0.0005 0.0014 0.0015 0.0043 0.0027 0.0074 0.0017 0.0024
Endoplasmic Reticulum 0.6927 0.5712 0.6415 0.6702 0.6555 0.7288 0.7043 0.5966 0.6555 0.8479 0.6351 0.7571 0.7233 0.5062 0.6604 0.7408 0.7019 0.6921
Endosome 0.0248 0.0207 0.0145 0.0264 0.0451 0.0127 0.0219 0.0073 0.0141 0.0037 0.0644 0.0123 0.0174 0.0057 0.0042 0.0038 0.0055 0.0078
Golgi 0.024 0.006 0.0223 0.0096 0.0187 0.0134 0.0315 0.0064 0.0153 0.0066 0.0149 0.0093 0.0136 0.0034 0.0085 0.0264 0.0054 0.0118
Lipid Particles 0.0194 0.0105 0.0117 0.0353 0.0074 0.0176 0.0285 0.0154 0.0221 0.0036 0.0102 0.009 0.0117 0.0168 0.0181 0.0264 0.0091 0.0065
Mitochondria 0.009 0.0021 0.0019 0.0021 0.0029 0.0032 0.0045 0.0007 0.0086 0.0006 0.0015 0.0042 0.0068 0.0012 0.0028 0.0005 0.0151 0.0063
None 0.0061 0.0024 0.0017 0.0011 0.0587 0.0016 0.0018 0.0032 0.0012 0.0003 0.001 0.0014 0.0097 0.0058 0.0057 0.0015 0.012 0.0054
Nuclear Periphery 0.0433 0.0229 0.0357 0.0493 0.0154 0.0153 0.0358 0.0162 0.0203 0.0134 0.0317 0.0127 0.0495 0.0374 0.025 0.0291 0.0148 0.0184
Nucleolus 0.0007 0.0005 0.0004 0.0002 0.0004 0.0002 0.0002 0.0005 0.0004 0.0001 0.0008 0.0001 0.0004 0.0009 0.0023 0.0002 0.001 0.0003
Nucleus 0.0035 0.0051 0.003 0.0032 0.0032 0.0021 0.0032 0.0023 0.0028 0.0011 0.008 0.0021 0.0047 0.0096 0.0082 0.0022 0.0029 0.0037
Peroxisomes 0.003 0.0005 0.0002 0.0002 0.0005 0.0011 0.0058 0.0001 0.0009 0 0.0003 0.0001 0.0011 0.0003 0.0002 0 0.0005 0.0002
Punctate Nuclear 0.0088 0.0009 0.0008 0.0006 0.0021 0.0008 0.0013 0.0006 0.0042 0.0001 0.0023 0.0003 0.0029 0.0018 0.0015 0.0006 0.0011 0.0005
Vacuole 0.0218 0.0181 0.0125 0.0228 0.0164 0.0089 0.0112 0.0167 0.0162 0.0084 0.0296 0.0097 0.0179 0.0305 0.0143 0.0128 0.0055 0.0146
Vacuole Periphery 0.0078 0.0034 0.0026 0.0092 0.0029 0.0017 0.0029 0.001 0.0033 0.0014 0.0048 0.0018 0.0047 0.0035 0.0016 0.0017 0.002 0.002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.3361 4.1006 5.809 23.9773 38.3876 7.9188 4.7115 4.7063 14.8063 68.6169
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2348 1223 1376 1956 1715 1402 1500 175 4063 2625 2876 2131

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1264.34 1477.49 2431.35 1683.61 1361.63 1406.85 1906.01 1459.79 1305.41 1439.76 2157.35 1665.23
Standard Deviation 610.75 601.92 1104.28 659.24 524.03 544.18 782.03 539.73 577.74 572.89 985.53 653.15
Intensity Change Log 2 0.224764 0.943373 0.413174 0.047134 0.485221 0.100426 0.135391 0.723967 0.259475

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.009547 0.008665 0.004068 0.007589 0.009408 0.014223 0.003807 0.010094 0.009488 0.011633 0.003932 0.007795
Bud Neck 0.016764 0.009716 0.023249 0.011827 0.009744 0.010720 0.012219 0.010712 0.013801 0.010252 0.017496 0.011735
Bud Site 0.197312* 0.108716* 0.062040 0.095926 0.105137* 0.141858* 0.056437 0.066064 0.158405* 0.126417* 0.059118 0.093474
Cell Periphery 0.108981* 0.071368 0.164175* 0.059138 0.070299 0.037800 0.025886 0.017339 0.092654 0.053439 0.092049 0.055705
Cytoplasm 0.057064 0.031336 0.016481 0.072196 0.033262 0.061529 0.032313 0.074104 0.047017 0.047462 0.024738 0.072352
Cytoplasmic Foci 0.008897 0.008806 0.002612 0.006886 0.005683 0.019297 0.004610 0.018698 0.007540 0.014409 0.003654 0.007856
Eisosomes 0.001064 0.001030 0.001065 0.000425 0.001054 0.000562 0.000210 0.000309 0.001060 0.000780 0.000619 0.000415
Endoplasmic Reticulum 0.494367* 0.680780* 0.508875* 0.613972* 0.700192* 0.605939* 0.750495* 0.607324* 0.581246* 0.640808* 0.634894* 0.613426*
Endosome 0.016451 0.017175 0.016299 0.012202 0.014173 0.025881 0.017478 0.022320 0.015490 0.021825 0.016914 0.013033
Golgi 0.002646 0.005106 0.005914 0.005929 0.003856 0.008790 0.001693 0.004237 0.003157 0.007073 0.003712 0.005790
Lipid Particles 0.006572 0.012172 0.040227 0.026127 0.009460 0.012949 0.011144 0.051631 0.007791 0.012587 0.025059 0.028221
Mitochondria 0.012987 0.004491 0.003993 0.003919 0.004538 0.008781 0.002927 0.000750 0.009421 0.006782 0.003437 0.003659
Mitotic Spindle 0.000565 0.001505 0.000797 0.003959 0.000387 0.001809 0.001488 0.003637 0.000490 0.001667 0.001157 0.003933
None 0.008767 0.002418 0.002521 0.001855 0.002669 0.005526 0.002606 0.000466 0.006193 0.004078 0.002565 0.001741
Nuclear Periphery 0.000902 0.000652 0.008415 0.001786 0.000512 0.000709 0.006177 0.002013 0.000737 0.000682 0.007248 0.001805
Nuclear Periphery Foci 0.003756 0.005031 0.015735 0.009289 0.002525 0.006720 0.021666 0.023691 0.003237 0.005933 0.018829 0.010471
Nucleolus 0.000276 0.000148 0.000147 0.000107 0.000084 0.000146 0.000106 0.000048 0.000195 0.000147 0.000126 0.000102
Nucleus 0.006720 0.002345 0.005771 0.010967 0.002281 0.004018 0.005967 0.008120 0.004846 0.003239 0.005873 0.010734
Peroxisomes 0.001686 0.001310 0.000138 0.000520 0.001666 0.002096 0.000137 0.000840 0.001677 0.001730 0.000138 0.000546
Vacuole 0.037308 0.022446 0.110148 0.051495 0.019716 0.025949 0.040706 0.075843 0.029883 0.024317 0.073930 0.053495
Vacuole Periphery 0.007367 0.004784 0.007331 0.003886 0.003352 0.004700 0.001928 0.001762 0.005672 0.004739 0.004513 0.003711

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.72 7.29 0.59 -0.13 -4.40 -2.77 4.36 -0.21 1.00 -1.48 -2.13 7.96 0.44 2.12 -4.74
Bud Neck 5.03 -5.30 3.15 -2.39 7.62 -0.84 -2.91 -0.86 -0.44 0.67 3.77 -5.21 0.94 -2.48 5.58
Bud Site 10.12 18.04* 12.51 0.54 -5.88 -4.41 6.42 2.57 5.08 -0.65 5.16 18.45 9.52 3.91 -7.00
Cell Periphery 7.61 -8.84 11.94 3.15 18.44 8.77 12.26 13.59 6.45 3.24 12.58 0.35 11.19 -0.08 9.81
Cytoplasm 6.42 11.17 -5.52 -11.25 -15.63 -6.32 -0.81 -4.27 -1.95 -4.01 -0.23 7.18 -8.91 -8.32 -14.01
Cytoplasmic Foci 0.04 6.49 1.28 0.89 -5.18 -7.41 0.81 -3.49 -0.08 -3.66 -5.75 4.92 -1.16 4.48 -5.10
Eisosomes 0.20 0.03 5.94 4.78 6.60 4.06 7.60 6.17 3.11 -1.71 2.88 5.16 7.84 4.90 3.46
Endoplasmic Reticulum -14.41 0.63 -7.11 7.46 -7.06 7.24 -2.13 4.57 1.46 5.37 -6.19 -3.33 0.40 5.56 3.14
Endosome -0.61 -0.72 4.71 3.97 5.51 -8.02 -3.86 -2.77 1.38 -1.17 -6.52 -3.23 3.15 8.44 5.86
Golgi -3.52 -7.11 -3.44 -0.47 0.23 -4.54 4.01 -0.05 3.76 -2.46 -5.99 -1.61 -2.90 1.61 -2.23
Lipid Particles -3.86 -12.02 -10.66 -6.32 4.58 -2.44 -1.72 -4.51 -4.10 -4.18 -4.76 -11.28 -11.11 -7.82 -0.86
Mitochondria 7.92 8.78 9.30 0.73 1.00 -3.32 2.29 8.42 6.57 7.32 2.98 9.18 9.07 4.02 0.44
Mitotic Spindle -1.64 -1.98 -4.43 -2.77 -3.90 -4.31 -3.24 -1.95 -1.23 -1.19 -3.67 -3.67 -4.81 -3.12 -3.50
None 6.28 6.69 7.79 0.87 1.39 -2.56 0.01 2.95 4.95 3.14 2.67 5.50 7.19 3.66 1.90
Nuclear Periphery 2.86 -16.41 -6.53 -9.20 14.23 -2.00 -9.25 -4.66 -3.93 5.92 0.74 -16.79 -9.14 -9.26 13.61
Nuclear Periphery Foci -1.48 -10.08 -7.41 -3.95 4.93 -5.07 -11.67 -5.64 -4.50 -0.61 -4.35 -15.16 -9.92 -5.19 6.73
Nucleolus 3.25 4.64 6.76 1.04 3.24 -2.86 -0.99 0.69 2.73 1.13 1.99 0.79 5.41 1.81 1.17
Nucleus 9.15 -1.09 -7.25 -11.77 -5.90 -3.91 -8.02 -3.87 -3.15 -1.97 3.98 -5.18 -9.74 -11.16 -6.60
Peroxisomes 1.14 6.81 5.06 3.41 -7.76 -0.82 8.27 2.66 2.26 -2.85 -0.17 10.02 7.19 4.17 -7.47
Vacuole 6.10 -19.88 -7.86 -12.30 12.52 -2.86 -9.74 -6.24 -5.72 -4.06 3.24 -20.09 -12.25 -13.90 4.74
Vacuole Periphery 2.25 -0.03 4.30 1.03 5.95 -2.06 2.78 2.08 3.42 0.50 1.40 2.07 3.49 1.80 2.12