Standard name
Human Ortholog
Description Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.24 0.23 – 0.21 0.16 0.08 0.08 – 0.09 0.11 0.05 0.09 – – – 0.5 0.51 0.47 0.31 0.25 0.19 0 0 0.07
Bud 0.07 0.06 – 0 0 0 0 – 0.08 0.05 0.1 0.05 – – – 0.08 0.09 0.05 0 0 0 0 0 0.05
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0.06 0.05 0 0 0.05
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0.05 0.19 – 0.15 0.14 0.09 0.05 – 0 0 0 0.09 – – – 0.3 0.32 0.17 0.1 0.11 0.14 0 0 0
Cytoplasm 0.33 0.33 – 0.55 0.47 0.55 0.54 – 0.38 0.45 0.28 0.34 – – – 0.18 0.14 0.2 0 0.05 0 0 0.09 0.11
Endoplasmic Reticulum 0.34 0.33 – 0.08 0.28 0.16 0.15 – 0.14 0.11 0.07 0.18 – – – 0.06 0.06 0.06 0.34 0.3 0.18 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0.06 0 0 – – – 0 0 0 0 0 0.05 0 0.1 0.08
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.43 0.09 0
Mitochondria 0.17 0.07 – 0.11 0 0.21 0.23 – 0.47 0.25 0.69 0.45 – – – 0.06 0.08 0.14 0.13 0.1 0.24 0.5 0.19 0.16
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.07
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0.05 0 0 – – – 0 0 0.05 0 0 0 0 0.13 0.07
Vac/Vac Membrane 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0.06 0 0 0.13 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 42 36 – 25 27 14 19 – 15 20 9 19 – – – 117 72 67 65 52 10 0 0 1
Bud 12 9 – 1 5 6 7 – 13 9 19 11 – – – 20 13 7 2 2 1 0 0 1
Bud Neck 0 2 – 0 3 0 2 – 0 0 0 2 – – – 0 0 0 7 12 3 0 0 1
Bud Site 0 0 – 0 0 1 0 – 2 2 2 0 – – – 0 0 0 – – – – – –
Cell Periphery 8 31 – 18 23 16 11 – 7 6 6 20 – – – 70 46 24 21 23 7 0 0 0
Cytoplasm 57 53 – 67 78 99 125 – 63 84 55 74 – – – 42 20 28 3 10 0 0 1 2
Endoplasmic Reticulum 58 53 – 10 46 28 34 – 24 21 14 40 – – – 14 8 9 71 62 10 0 0 0
Endosome 4 0 – 2 1 4 5 – 6 12 5 6 – – – 4 3 3 3 2 2 0 1 1
Golgi 0 2 – 0 1 1 0 – 0 0 0 0 – – – 9 3 3 4 4 1 0 1 0
Mitochondria 30 11 – 13 7 37 53 – 79 47 137 98 – – – 13 12 20 26 20 13 0 2 3
Nucleus 0 0 – 1 2 0 2 – 1 2 2 2 – – – 4 3 4 0 0 0 0 0 1
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 1 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 1
Peroxisomes 2 3 – 1 0 1 2 – 1 3 1 2 – – – 4 0 1 1 0 1 0 0 0
SpindlePole 1 0 – 0 4 2 8 – 1 9 0 7 – – – 10 4 7 0 0 0 0 1 1
Vac/Vac Membrane 0 2 – 2 5 4 7 – 4 7 3 6 – – – 6 0 2 4 12 1 0 1 1
Unique Cell Count 172 160 121 166 180 232 167 185 199 219 236 142 143 212 209 55 2 13 22
Labelled Cell Count 214 202 140 202 213 275 216 222 253 288 313 184 175 212 209 55 2 13 22


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 6.8 – 6.5 6.9 5.7 5.7 – 5.0 5.2 4.6 4.8 – – – 8.5 8.2 7.7 – – –
Std Deviation (1e-4) 0.6 1.3 – 1.7 1.7 1.7 1.5 – 1.7 1.3 1.5 0.9 – – – 1.5 1.3 1.3 – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.834 0.6999 0.6261 0.6013 0.4202 0.6995 0.5331 0.8132 0.8092 0.9758 0.6132 0.7693 -0.2467 0.2805 0.0013 0.0688 -0.2247 0.091
Actin 0.1094 0.0567 0.0975 0.1131 0.1804 0.0666 0.0825 0.0235 0.0618 0.118 0.0296 0.0413 0.0449 0.0302 0.1067 0.2286 0.0409 0.049
Bud 0.0023 0.0037 0.0083 0.0064 0.0041 0.0031 0.0012 0.0008 0.0031 0.0042 0.0088 0.0017 0.0029 0.0005 0.0044 0.0014 0.0023 0.0009
Bud Neck 0.0131 0.0032 0.0232 0.0413 0.0106 0.0445 0.0218 0.0066 0.0199 0.0355 0.0047 0.0733 0.0051 0.007 0.0197 0.0085 0.0263 0.0494
Bud Periphery 0.0052 0.0071 0.0217 0.0464 0.0185 0.0131 0.0028 0.002 0.013 0.0412 0.0231 0.0083 0.014 0.0019 0.0249 0.0068 0.0118 0.0028
Bud Site 0.0319 0.07 0.0397 0.0193 0.0061 0.0026 0.022 0.104 0.0553 0.0065 0.005 0.0032 0.0261 0.0805 0.0361 0.0069 0.0102 0.0064
Cell Periphery 0.0223 0.0146 0.0187 0.016 0.0146 0.0151 0.0226 0.0257 0.0208 0.0268 0.0236 0.0347 0.0403 0.0312 0.0272 0.0234 0.0542 0.0138
Cytoplasm 0.2935 0.3437 0.3273 0.3523 0.2773 0.4088 0.3497 0.443 0.3829 0.3919 0.419 0.4734 0.4717 0.4524 0.4088 0.4644 0.5657 0.4731
Cytoplasmic Foci 0.0814 0.0477 0.0537 0.0408 0.0532 0.0715 0.0646 0.0411 0.0504 0.0487 0.0498 0.0465 0.0534 0.0448 0.045 0.0308 0.0518 0.0832
Eisosomes 0.0056 0.0029 0.0055 0.0035 0.0063 0.0033 0.006 0.0025 0.0031 0.0046 0.0015 0.0022 0.0051 0.0028 0.0045 0.0044 0.0009 0.0027
Endoplasmic Reticulum 0.0225 0.0318 0.0256 0.0488 0.0553 0.0326 0.0296 0.0239 0.0284 0.055 0.0266 0.0243 0.0339 0.018 0.0347 0.0264 0.011 0.0267
Endosome 0.0351 0.0179 0.0221 0.0245 0.0389 0.0296 0.0294 0.0161 0.0326 0.0124 0.043 0.0242 0.0135 0.0171 0.0167 0.0065 0.0642 0.0245
Golgi 0.026 0.0124 0.0202 0.0172 0.0337 0.0173 0.0158 0.0174 0.0245 0.0176 0.0206 0.0128 0.013 0.0156 0.0212 0.0142 0.0137 0.0195
Lipid Particles 0.0266 0.0129 0.0175 0.0165 0.0178 0.0129 0.0468 0.0239 0.0274 0.0211 0.0237 0.0116 0.0395 0.0082 0.0117 0.0065 0.0082 0.0246
Mitochondria 0.0095 0.0057 0.0099 0.0192 0.0204 0.0067 0.0073 0.0019 0.0151 0.0088 0.0869 0.019 0.0037 0.0017 0.0055 0.0034 0.0069 0.0067
None 0.2585 0.3457 0.2793 0.2053 0.2077 0.2492 0.2465 0.2574 0.234 0.1493 0.2011 0.2013 0.2105 0.2785 0.2201 0.1564 0.074 0.2026
Nuclear Periphery 0.0084 0.0017 0.0018 0.0018 0.0053 0.0037 0.0016 0.0008 0.0014 0.0016 0.005 0.0076 0.0015 0.0007 0.0022 0.0008 0.0244 0.0005
Nucleolus 0.0024 0.0014 0.0019 0.0009 0.0023 0.001 0.0052 0.0005 0.0008 0.0005 0.0019 0.0006 0.0006 0.0003 0.0004 0.0002 0.0005 0.0004
Nucleus 0.006 0.0035 0.0043 0.0016 0.006 0.0042 0.0024 0.0012 0.0016 0.0013 0.0042 0.0041 0.0009 0.0008 0.0009 0.0007 0.0044 0.0008
Peroxisomes 0.0183 0.0047 0.0081 0.019 0.0275 0.0067 0.03 0.0022 0.0167 0.0499 0.0092 0.0034 0.0136 0.0034 0.0041 0.0062 0.0065 0.0072
Punctate Nuclear 0.0135 0.0068 0.0072 0.0022 0.0075 0.0027 0.0027 0.001 0.0017 0.0011 0.0034 0.002 0.0014 0.0007 0.0013 0.0004 0.0158 0.0013
Vacuole 0.0073 0.0051 0.0058 0.003 0.0049 0.0041 0.0082 0.004 0.0048 0.0038 0.007 0.0032 0.0042 0.0034 0.0035 0.0027 0.0048 0.0033
Vacuole Periphery 0.0012 0.0006 0.0009 0.0008 0.0015 0.0008 0.0014 0.0002 0.0007 0.0005 0.0024 0.0013 0.0003 0.0002 0.0005 0.0003 0.0017 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.4485 7.2692 9.9583 10.565 10.9486 8.1272 9.7527 7.2502 6.872 11.8722
Translational Efficiency 0.3372 0.574 0.3723 0.341 0.3322 0.4004 0.4532 0.3277 0.4725 0.3577

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2154 1046 190 379 1520 1107 737 1010 3674 2153 927 1389

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 673.87 703.14 1041.59 898.66 761.80 776.89 977.40 914.01 710.25 741.06 990.56 909.82
Standard Deviation 72.65 111.12 109.94 109.70 91.34 102.46 111.58 118.03 91.77 112.94 114.23 116.02
Intensity Change Log 2 — 0.061342 0.628245 0.415305 — 0.028298 0.359537 0.262798 — 0.043902 0.491910 0.336392

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.016430 0.043464 0.019282 0.148396* 0.038600 0.016543 0.046993 0.048838 0.025602 0.029622 0.041313 0.076003
Bud Neck 0.025340 0.026112 0.011842 0.022498 0.020371 0.036871 0.013236 0.021578 0.023284 0.031644 0.012950 0.021829
Bud Site 0.010870 0.030923 0.036162 0.090522 0.024013 0.030182 0.045533 0.051950 0.016307 0.030542 0.043612 0.062475
Cell Periphery 0.005237 0.013372 0.003330 0.025708 0.014136 0.004587 0.011829 0.007301 0.008918 0.008855 0.010087 0.012323
Cytoplasm 0.256100* 0.195714* 0.251384* 0.219220* 0.357472* 0.274174* 0.354804* 0.289330* 0.298040* 0.236055* 0.333607* 0.270200*
Cytoplasmic Foci 0.170400 0.179040 0.111714 0.071314 0.147895 0.209212* 0.084466 0.125047 0.161089 0.194553* 0.090051 0.110385
Eisosomes 0.029317 0.030138 0.000814 0.030950 0.049675 0.014521 0.016733 0.014697 0.037740 0.022108 0.013470 0.019132
Endoplasmic Reticulum 0.029939 0.035188 0.079129 0.108137* 0.037942 0.023620 0.105545* 0.049152 0.033250 0.029240 0.100131* 0.065246
Endosome 0.018821 0.038900 0.022591 0.008728 0.010921 0.042167 0.008604 0.019687 0.015553 0.040580 0.011470 0.016697
Golgi 0.122046* 0.126801* 0.002210 0.010760 0.100982 0.086713 0.007545 0.011647 0.113332* 0.106189 0.006451 0.011405
Lipid Particles 0.132823* 0.098193 0.037035 0.039396 0.066730 0.091086 0.038448 0.052733 0.105479* 0.094539 0.038159 0.049094
Mitochondria 0.052498 0.069766 0.000307 0.002546 0.021307 0.052422 0.000775 0.003884 0.039594 0.060848 0.000679 0.003519
Mitotic Spindle 0.000228 0.000300 0.002692 0.001048 0.000499 0.003379 0.001733 0.010816 0.000340 0.001883 0.001930 0.008151
None 0.006629 0.001299 0.000583 0.000471 0.001912 0.001252 0.000650 0.001265 0.004677 0.001275 0.000636 0.001048
Nuclear Periphery 0.000242 0.000293 0.001946 0.000338 0.000154 0.000423 0.000749 0.000672 0.000205 0.000360 0.000994 0.000581
Nuclear Periphery Foci 0.000434 0.000932 0.043468 0.006376 0.000706 0.000779 0.016212 0.008561 0.000546 0.000853 0.021798 0.007965
Nucleolus 0.000449 0.000681 0.000342 0.000101 0.000175 0.000740 0.000144 0.000269 0.000335 0.000711 0.000185 0.000223
Nucleus 0.023525 0.009330 0.126463 0.067218 0.025262 0.023922 0.139839* 0.086034 0.024244 0.016832 0.137097* 0.080900
Peroxisomes 0.083066 0.076253 0.003016 0.018503 0.069189 0.043630 0.007515 0.019673 0.077325 0.059479 0.006593 0.019353
Vacuole 0.012729 0.017201 0.245344* 0.126782 0.010813 0.037544 0.098460 0.175109 0.011936 0.027661 0.128565 0.161922
Vacuole Periphery 0.002878 0.006098 0.000347 0.000988 0.001248 0.006234 0.000187 0.001758 0.002204 0.006168 0.000220 0.001548

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.70 -0.19 -11.55 -8.70 -10.61 7.47 -1.36 -1.50 -6.96 -0.00 -1.81 -3.72 -10.58 -9.18 -5.85
Bud Neck -0.32 5.44 1.42 1.58 -3.56 -6.23 4.18 -0.07 6.03 -4.04 -4.58 7.28 1.53 5.65 -5.21
Bud Site -9.45 -7.02 -10.85 -7.83 -6.72 -2.23 -5.28 -5.96 -4.34 -0.76 -8.40 -8.71 -12.30 -7.90 -3.69
Cell Periphery -5.61 3.76 -6.85 -3.55 -7.64 9.13 1.88 6.39 -2.84 3.50 0.08 -0.70 -2.51 -2.31 -1.43
Cytoplasm 8.53 1.17 3.54 -1.82 1.23 9.53 0.83 8.13 -0.87 5.93 10.87 -3.40 4.76 -3.84 6.40
Cytoplasmic Foci -1.45 8.95 15.68 14.72 3.86 -9.80 12.24 4.22 12.87 -6.97 -7.74 18.03 11.98 17.01 -4.39
Eisosomes -0.33 21.15 0.06 0.28 -9.49 13.97 11.77 13.80 0.64 1.08 9.11 13.10 11.16 2.50 -2.49
Endoplasmic Reticulum -1.73 -5.88 -8.13 -7.40 -2.59 4.88 -9.84 -1.93 -5.57 7.95 1.93 -11.93 -7.41 -8.28 5.55
Endosome -9.54 -1.94 5.75 11.92 6.27 -15.59 2.32 -4.06 9.19 -5.23 -17.14 4.93 -0.15 12.93 -2.93
Golgi -0.91 40.62* 35.46* 26.35 -6.81 3.21 29.23 27.29 22.25 -2.18 2.08 47.35* 43.87* 33.68 -3.60
Lipid Particles 6.16 20.35 19.69 10.27 -0.86 -5.21 7.88 4.40 8.22 -3.03 2.88 21.40 18.29 12.39 -2.97
Mitochondria -3.90 24.33 19.94 16.50 -1.55 -9.81 15.74 9.90 15.53 -2.19 -7.65 27.92 21.46 22.24 -2.57
Mitotic Spindle -0.60 -2.08 -2.21 -1.96 1.22 -2.76 -2.03 -4.55 -2.97 -3.64 -2.87 -2.81 -4.73 -3.60 -3.36
None 6.01 7.01 6.92 2.53 0.30 1.50 4.20 1.53 0.11 -1.43 6.11 7.71 6.26 0.82 -1.27
Nuclear Periphery -0.97 -8.95 -1.79 -0.71 8.06 -3.83 -10.89 -10.47 -3.30 0.87 -3.43 -13.11 -9.62 -4.14 5.73
Nuclear Periphery Foci -4.41 -11.11 -11.08 -9.98 9.34 -0.64 -10.38 -10.53 -10.34 4.61 -3.79 -14.39 -13.24 -12.59 8.61
Nucleolus -0.59 2.36 11.75 1.48 6.79 -2.97 1.58 -3.63 2.45 -4.61 -1.79 6.89 4.30 2.28 -1.84
Nucleus 11.42 -10.09 -8.16 -10.86 5.37 0.63 -19.32 -15.97 -15.72 7.81 6.01 -22.10 -18.37 -20.46 9.59
Peroxisomes 1.66 31.87* 22.00 15.93 -8.41 7.75 23.23 16.76 9.74 -6.47 6.74 37.80* 27.07 17.92 -8.73
Vacuole -2.77 -17.58 -13.22 -12.71 7.00 -10.48 -18.46 -26.78 -21.98 -10.23 -10.16 -23.29 -29.62 -26.34 -5.24
Vacuole Periphery -6.70 12.44 3.57 7.72 -1.11 -8.99 10.82 -0.64 6.35 -2.82 -10.61 15.48 2.02 9.18 -3.06
Standard name
Human Ortholog
Description Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p
Localization
Cell Percentages ER (60%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Slp1

Slp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Slp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available