Standard name
Description Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.24 0.23 – 0.21 0.16 0.08 0.08 – 0.09 0.11 0.05 0.09 – – – 0.5 0.51 0.47 0.31 0.25 0.19 0 0 0.07
Bud 0.07 0.06 – 0 0 0 0 – 0.08 0.05 0.1 0.05 – – – 0.08 0.09 0.05 0 0 0 0 0 0.05
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0.06 0.05 0 0 0.05
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0.05 0.19 – 0.15 0.14 0.09 0.05 – 0 0 0 0.09 – – – 0.3 0.32 0.17 0.1 0.11 0.14 0 0 0
Cytoplasm 0.33 0.33 – 0.55 0.47 0.55 0.54 – 0.38 0.45 0.28 0.34 – – – 0.18 0.14 0.2 0 0.05 0 0 0.09 0.11
Endoplasmic Reticulum 0.34 0.33 – 0.08 0.28 0.16 0.15 – 0.14 0.11 0.07 0.18 – – – 0.06 0.06 0.06 0.34 0.3 0.18 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0.06 0 0 – – – 0 0 0 0 0 0.05 0 0.1 0.08
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.43 0.09 0
Mitochondria 0.17 0.07 – 0.11 0 0.21 0.23 – 0.47 0.25 0.69 0.45 – – – 0.06 0.08 0.14 0.13 0.1 0.24 0.5 0.19 0.16
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.07
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0.05 0 0 – – – 0 0 0.05 0 0 0 0 0.13 0.07
Vac/Vac Membrane 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0.06 0 0 0.13 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 42 36 – 25 27 14 19 – 15 20 9 19 – – – 117 72 67 65 52 10 0 0 1
Bud 12 9 – 1 5 6 7 – 13 9 19 11 – – – 20 13 7 2 2 1 0 0 1
Bud Neck 0 2 – 0 3 0 2 – 0 0 0 2 – – – 0 0 0 7 12 3 0 0 1
Bud Site 0 0 – 0 0 1 0 – 2 2 2 0 – – – 0 0 0 – – – – – –
Cell Periphery 8 31 – 18 23 16 11 – 7 6 6 20 – – – 70 46 24 21 23 7 0 0 0
Cytoplasm 57 53 – 67 78 99 125 – 63 84 55 74 – – – 42 20 28 3 10 0 0 1 2
Endoplasmic Reticulum 58 53 – 10 46 28 34 – 24 21 14 40 – – – 14 8 9 71 62 10 0 0 0
Endosome 4 0 – 2 1 4 5 – 6 12 5 6 – – – 4 3 3 3 2 2 0 1 1
Golgi 0 2 – 0 1 1 0 – 0 0 0 0 – – – 9 3 3 4 4 1 0 1 0
Mitochondria 30 11 – 13 7 37 53 – 79 47 137 98 – – – 13 12 20 26 20 13 0 2 3
Nucleus 0 0 – 1 2 0 2 – 1 2 2 2 – – – 4 3 4 0 0 0 0 0 1
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 1 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 1
Peroxisomes 2 3 – 1 0 1 2 – 1 3 1 2 – – – 4 0 1 1 0 1 0 0 0
SpindlePole 1 0 – 0 4 2 8 – 1 9 0 7 – – – 10 4 7 0 0 0 0 1 1
Vac/Vac Membrane 0 2 – 2 5 4 7 – 4 7 3 6 – – – 6 0 2 4 12 1 0 1 1
Unique Cell Count 172 160 121 166 180 232 167 185 199 219 236 142 143 212 209 55 2 13 22
Labelled Cell Count 214 202 140 202 213 275 216 222 253 288 313 184 175 212 209 55 2 13 22


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 6.8 – 6.5 6.9 5.7 5.7 – 5.0 5.2 4.6 4.8 – – – 8.5 8.2 7.7 – – –
Std Deviation (1e-4) 0.6 1.3 – 1.7 1.7 1.7 1.5 – 1.7 1.3 1.5 0.9 – – – 1.5 1.3 1.3 – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.834 0.6999 0.6261 0.6013 0.4202 0.6995 0.5331 0.8132 0.8092 0.9758 0.6132 0.7693 -0.2467 0.2805 0.0013 0.0688 -0.2247 0.091
Actin 0.1094 0.0567 0.0975 0.1131 0.1804 0.0666 0.0825 0.0235 0.0618 0.118 0.0296 0.0413 0.0449 0.0302 0.1067 0.2286 0.0409 0.049
Bud 0.0023 0.0037 0.0083 0.0064 0.0041 0.0031 0.0012 0.0008 0.0031 0.0042 0.0088 0.0017 0.0029 0.0005 0.0044 0.0014 0.0023 0.0009
Bud Neck 0.0131 0.0032 0.0232 0.0413 0.0106 0.0445 0.0218 0.0066 0.0199 0.0355 0.0047 0.0733 0.0051 0.007 0.0197 0.0085 0.0263 0.0494
Bud Periphery 0.0052 0.0071 0.0217 0.0464 0.0185 0.0131 0.0028 0.002 0.013 0.0412 0.0231 0.0083 0.014 0.0019 0.0249 0.0068 0.0118 0.0028
Bud Site 0.0319 0.07 0.0397 0.0193 0.0061 0.0026 0.022 0.104 0.0553 0.0065 0.005 0.0032 0.0261 0.0805 0.0361 0.0069 0.0102 0.0064
Cell Periphery 0.0223 0.0146 0.0187 0.016 0.0146 0.0151 0.0226 0.0257 0.0208 0.0268 0.0236 0.0347 0.0403 0.0312 0.0272 0.0234 0.0542 0.0138
Cytoplasm 0.2935 0.3437 0.3273 0.3523 0.2773 0.4088 0.3497 0.443 0.3829 0.3919 0.419 0.4734 0.4717 0.4524 0.4088 0.4644 0.5657 0.4731
Cytoplasmic Foci 0.0814 0.0477 0.0537 0.0408 0.0532 0.0715 0.0646 0.0411 0.0504 0.0487 0.0498 0.0465 0.0534 0.0448 0.045 0.0308 0.0518 0.0832
Eisosomes 0.0056 0.0029 0.0055 0.0035 0.0063 0.0033 0.006 0.0025 0.0031 0.0046 0.0015 0.0022 0.0051 0.0028 0.0045 0.0044 0.0009 0.0027
Endoplasmic Reticulum 0.0225 0.0318 0.0256 0.0488 0.0553 0.0326 0.0296 0.0239 0.0284 0.055 0.0266 0.0243 0.0339 0.018 0.0347 0.0264 0.011 0.0267
Endosome 0.0351 0.0179 0.0221 0.0245 0.0389 0.0296 0.0294 0.0161 0.0326 0.0124 0.043 0.0242 0.0135 0.0171 0.0167 0.0065 0.0642 0.0245
Golgi 0.026 0.0124 0.0202 0.0172 0.0337 0.0173 0.0158 0.0174 0.0245 0.0176 0.0206 0.0128 0.013 0.0156 0.0212 0.0142 0.0137 0.0195
Lipid Particles 0.0266 0.0129 0.0175 0.0165 0.0178 0.0129 0.0468 0.0239 0.0274 0.0211 0.0237 0.0116 0.0395 0.0082 0.0117 0.0065 0.0082 0.0246
Mitochondria 0.0095 0.0057 0.0099 0.0192 0.0204 0.0067 0.0073 0.0019 0.0151 0.0088 0.0869 0.019 0.0037 0.0017 0.0055 0.0034 0.0069 0.0067
None 0.2585 0.3457 0.2793 0.2053 0.2077 0.2492 0.2465 0.2574 0.234 0.1493 0.2011 0.2013 0.2105 0.2785 0.2201 0.1564 0.074 0.2026
Nuclear Periphery 0.0084 0.0017 0.0018 0.0018 0.0053 0.0037 0.0016 0.0008 0.0014 0.0016 0.005 0.0076 0.0015 0.0007 0.0022 0.0008 0.0244 0.0005
Nucleolus 0.0024 0.0014 0.0019 0.0009 0.0023 0.001 0.0052 0.0005 0.0008 0.0005 0.0019 0.0006 0.0006 0.0003 0.0004 0.0002 0.0005 0.0004
Nucleus 0.006 0.0035 0.0043 0.0016 0.006 0.0042 0.0024 0.0012 0.0016 0.0013 0.0042 0.0041 0.0009 0.0008 0.0009 0.0007 0.0044 0.0008
Peroxisomes 0.0183 0.0047 0.0081 0.019 0.0275 0.0067 0.03 0.0022 0.0167 0.0499 0.0092 0.0034 0.0136 0.0034 0.0041 0.0062 0.0065 0.0072
Punctate Nuclear 0.0135 0.0068 0.0072 0.0022 0.0075 0.0027 0.0027 0.001 0.0017 0.0011 0.0034 0.002 0.0014 0.0007 0.0013 0.0004 0.0158 0.0013
Vacuole 0.0073 0.0051 0.0058 0.003 0.0049 0.0041 0.0082 0.004 0.0048 0.0038 0.007 0.0032 0.0042 0.0034 0.0035 0.0027 0.0048 0.0033
Vacuole Periphery 0.0012 0.0006 0.0009 0.0008 0.0015 0.0008 0.0014 0.0002 0.0007 0.0005 0.0024 0.0013 0.0003 0.0002 0.0005 0.0003 0.0017 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.4485 7.2692 9.9583 10.565 10.9486 8.1272 9.7527 7.2502 6.872 11.8722
Translational Efficiency 0.3372 0.574 0.3723 0.341 0.3322 0.4004 0.4532 0.3277 0.4725 0.3577

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘