Standard name
Human Ortholog
Description Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.09 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.11 0.32 0.27 0.2 0.17 0.17 0.16 0.09 0.11 0.1 0.1 0.15 0.2 0.26 0.08 0.13 0.15 0 0.05 0.09 0.08 0.12 0.13
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 0.43 0.07 0.18 0.11 0.56 0.54 0.39 0.68 0.73 0.77 0.59 0.2 0.11 0.06 0.05 0 0.08 0 0.06 0.05 0.06 0.08 0.08
Nucleus 0.53 0.55 0.56 0.48 0.62 0.45 0.5 0.54 0.53 0.49 0.53 0.57 0.56 0.59 0.54 0.71 0.69 0.67 0.47 0.55 0.38 0.34 0.24 0.25
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.12 0.09 0 0.06 0.07 0 0.05 0.05 0.05 0 0 0 0 0 0 0.1 0 0.06 0 0.06 0.07
Vac/Vac Membrane 0.14 0.21 0 0 0 0 0 0 0 0 0 0 0.15 0.14 0.17 0.11 0.15 0.16 0.17 0.23 0.3 0.39 0.39 0.34
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud 1 1 1 1 1 4 12 2 7 9 7 7 0 0 0 1 0 0 2 1 3 2 3 3
Bud Neck 1 0 19 8 13 4 11 16 3 6 3 5 0 0 0 0 0 0 0 0 2 0 1 2
Bud Site 0 0 1 1 3 6 6 7 10 14 14 16 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 15 14 69 49 57 53 79 80 32 42 25 32 20 31 42 10 18 14 8 7 34 8 21 23
Endoplasmic Reticulum 0 0 2 0 1 0 1 0 1 1 0 0 2 0 2 6 10 4 0 0 1 1 1 2
Endosome 2 1 0 3 4 0 3 2 0 1 1 1 3 1 4 5 4 0 7 2 8 0 3 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 1 1 0 0 0 0 0
Mitochondria 55 55 15 33 30 174 244 194 240 287 186 189 26 17 10 7 3 7 7 8 16 6 14 13
Nucleus 73 70 122 86 174 140 229 271 186 195 128 182 75 91 87 94 94 61 92 87 139 36 43 46
Nuclear Periphery 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 1 1 0 3 0 2 0 0 0
Nucleolus 0 0 0 0 0 1 4 3 3 3 3 1 0 0 0 1 0 0 8 3 5 2 2 5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
SpindlePole 0 1 5 21 26 7 27 35 12 20 11 17 0 0 2 3 2 1 20 4 22 3 10 12
Vac/Vac Membrane 19 27 8 4 7 7 7 16 5 5 0 6 20 21 27 15 21 15 32 36 110 42 71 62
Unique Cell Count 137 127 219 181 281 309 454 502 354 395 242 318 133 153 161 132 136 91 196 158 369 108 182 181
Labelled Cell Count 166 169 242 206 317 396 624 626 499 585 378 456 146 161 175 148 153 103 196 158 369 108 182 181


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.9 4.7 4.3 4.8 3.7 4.0 4.4 3.5 3.4 3.2 3.8 4.3 4.5 4.6 6.8 6.7 6.5 4.0 4.4 4.6
Std Deviation (1e-4) 0.9 0.7 0.9 0.9 1.0 1.0 1.1 1.3 1.0 1.0 0.7 0.8 0.9 1.1 1.4 1.6 1.2 1.4 1.3 2.2 2.0
Intensity Change (Log2) – – – -0.14 0.02 -0.36 -0.22 -0.1 -0.42 -0.44 -0.55 -0.31 -0.12 -0.07 -0.03 0.53 0.52 0.47 -0.21 -0.08 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.8 3.9 3.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4443 1.9623 2.051 1.4612 2.0443 2.3362 -0.2183 -0.3254 -0.4875 -0.2184 -0.805 -0.5126 -0.8297 -0.8864 -1.1178 -0.7893 -1.1378 -1.1884
Actin 0.0398 0.0008 0.0059 0.0044 0.0232 0.0034 0.0347 0.0004 0.0146 0.0152 0.0023 0.0006 0.0262 0.0005 0.0124 0.023 0.053 0.0042
Bud 0.0011 0.0006 0.0008 0.0002 0.0003 0.0003 0.0009 0.0002 0.0003 0.0003 0.0002 0.0001 0.0003 0.0003 0.0004 0.0012 0.0022 0.0001
Bud Neck 0.0085 0.0013 0.0019 0.0015 0.0031 0.0127 0.017 0.002 0.0022 0.0015 0.0014 0.0094 0.0019 0.001 0.0091 0.001 0.0016 0.0042
Bud Periphery 0.0019 0.0011 0.0009 0.0002 0.0004 0.0012 0.0019 0.0002 0.0004 0.0005 0.0008 0.0002 0.0003 0.0002 0.0005 0.0023 0.0035 0.0001
Bud Site 0.01 0.0031 0.0059 0.0016 0.0008 0.0008 0.0067 0.0056 0.0066 0.0009 0.0004 0.0003 0.002 0.0041 0.0031 0.0051 0.0068 0.0003
Cell Periphery 0.0006 0.0002 0.0002 0.0001 0.0004 0.0002 0.0004 0.0001 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0001 0.0002 0.0004 0
Cytoplasm 0.0874 0.0888 0.0499 0.0214 0.1023 0.0656 0.0565 0.08 0.0655 0.0241 0.0356 0.0656 0.0939 0.0811 0.0622 0.0584 0.0196 0.0642
Cytoplasmic Foci 0.0228 0.0041 0.0047 0.0247 0.0168 0.0053 0.0508 0.0102 0.0093 0.0157 0.0024 0.0063 0.017 0.008 0.0124 0.0211 0.0132 0.0112
Eisosomes 0.0008 0.0001 0.0002 0.0001 0.0002 0.0001 0.0004 0.0001 0.0003 0.0002 0.0001 0 0.0002 0.0001 0.0002 0.0002 0.0005 0.0001
Endoplasmic Reticulum 0.0075 0.0043 0.0024 0.0024 0.0087 0.0024 0.0076 0.0051 0.0065 0.0024 0.0025 0.0017 0.0055 0.0056 0.0043 0.0058 0.0018 0.0014
Endosome 0.0265 0.007 0.0119 0.0136 0.038 0.0031 0.0438 0.0059 0.0212 0.0271 0.0096 0.0066 0.0186 0.0061 0.0105 0.0127 0.0132 0.0054
Golgi 0.0165 0.0005 0.0045 0.0047 0.027 0.0014 0.0125 0.0003 0.0034 0.0064 0.0012 0.0017 0.0054 0.0003 0.0091 0.015 0.0129 0.0016
Lipid Particles 0.0334 0.0009 0.003 0.0168 0.016 0.0054 0.0225 0.001 0.0032 0.0173 0.0043 0.0055 0.0109 0.0024 0.0038 0.0133 0.0124 0.0062
Mitochondria 0.0295 0.0152 0.0219 0.019 0.0325 0.0177 0.0137 0.0006 0.003 0.0041 0.0054 0.0058 0.0017 0.0007 0.004 0.008 0.0122 0.0007
None 0.0896 0.1505 0.185 0.1414 0.1533 0.1864 0.0975 0.1262 0.1141 0.0767 0.056 0.0659 0.1402 0.2107 0.1487 0.2549 0.1263 0.1439
Nuclear Periphery 0.0298 0.0153 0.0198 0.0314 0.0491 0.0103 0.0427 0.0371 0.0443 0.0698 0.0654 0.0154 0.0225 0.0339 0.0326 0.0183 0.0252 0.0128
Nucleolus 0.0119 0.0207 0.0112 0.0101 0.013 0.0193 0.0089 0.0106 0.0087 0.006 0.0141 0.0141 0.0057 0.0148 0.0059 0.0066 0.0086 0.0125
Nucleus 0.4977 0.6137 0.5925 0.5351 0.4096 0.5655 0.4828 0.6195 0.6331 0.6578 0.6662 0.6981 0.5311 0.5642 0.5875 0.4806 0.5219 0.6621
Peroxisomes 0.0169 0.0014 0.0049 0.0378 0.0279 0.0052 0.0131 0.0004 0.0022 0.0327 0.0005 0.0013 0.0138 0.0002 0.0027 0.0176 0.0095 0.0046
Punctate Nuclear 0.0617 0.065 0.0691 0.1314 0.0688 0.0915 0.073 0.0903 0.0559 0.0354 0.1258 0.0994 0.0993 0.0631 0.0883 0.0529 0.1519 0.0629
Vacuole 0.0041 0.0046 0.0026 0.0015 0.0064 0.0019 0.007 0.0035 0.0035 0.0041 0.0024 0.0014 0.0026 0.0021 0.0017 0.0012 0.0021 0.0013
Vacuole Periphery 0.0022 0.0008 0.0009 0.0006 0.0022 0.0004 0.0056 0.0007 0.0015 0.0018 0.0032 0.0005 0.0006 0.0005 0.0006 0.0006 0.0011 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.8027 7.7029 8.4234 8.3119 6.468 5.8924 10.0429 10.0983 9.4593 8.8488
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
808 192 1141 1348 1865 1510 567 18 2673 1702 1708 1366

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 636.18 806.30 853.02 847.04 684.90 689.52 798.07 865.38 670.17 702.69 834.78 847.28
Standard Deviation 60.33 109.42 114.26 120.02 76.13 104.46 112.33 110.07 75.13 111.34 116.53 119.92
Intensity Change Log 2 — 0.341882 0.423145 0.412995 — 0.009699 0.220622 0.337440 — 0.179219 0.321701 0.374319

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000058 0.001220 0.000624 0.000774 0.000065 0.000519 0.000199 0.000573 0.000063 0.000598 0.000483 0.000771
Bud Neck 0.006121 0.014080 0.010995 0.012530 0.003526 0.012176 0.003412 0.002018 0.004310 0.012391 0.008478 0.012392
Bud Site 0.000642 0.013730 0.003042 0.014871 0.000620 0.009221 0.002935 0.001415 0.000627 0.009730 0.003006 0.014693
Cell Periphery 0.000141 0.000185 0.000078 0.000187 0.000090 0.000329 0.000987 0.000540 0.000105 0.000313 0.000380 0.000192
Cytoplasm 0.282580* 0.121516* 0.014375 0.104750* 0.256879* 0.213024* 0.143042* 0.086186 0.264648* 0.202701* 0.057088 0.104505*
Cytoplasmic Foci 0.114946 0.082060 0.000212 0.002087 0.060882 0.121281 0.013626 0.015687 0.077224 0.116857 0.004665 0.002266
Eisosomes 0.000101 0.000122 0.000023 0.000031 0.000044 0.000114 0.000952 0.000077 0.000061 0.000115 0.000332 0.000032
Endoplasmic Reticulum 0.000900 0.006200 0.006005 0.007587 0.001073 0.002661 0.002153 0.008392 0.001021 0.003060 0.004727 0.007598
Endosome 0.002240 0.008722 0.000423 0.003391 0.002060 0.007399 0.000760 0.001532 0.002114 0.007548 0.000535 0.003367
Golgi 0.000907 0.005789 0.000063 0.002850 0.000522 0.005724 0.000181 0.000082 0.000639 0.005732 0.000102 0.002814
Lipid Particles 0.018681 0.010080 0.000339 0.000724 0.006524 0.019363 0.001519 0.001606 0.010199 0.018315 0.000731 0.000735
Mitochondria 0.008082 0.008653 0.001120 0.002268 0.003799 0.028151 0.002528 0.001107 0.005094 0.025952 0.001587 0.002253
Mitotic Spindle 0.001071 0.018359 0.002836 0.042054 0.001603 0.016014 0.000552 0.031899 0.001442 0.016278 0.002078 0.041920
None 0.015613 0.005185 0.003263 0.010024 0.009301 0.017260 0.019501 0.062724 0.011209 0.015898 0.008654 0.010718
Nuclear Periphery 0.001955 0.002332 0.001149 0.003290 0.002498 0.003479 0.000838 0.001911 0.002334 0.003349 0.001045 0.003272
Nuclear Periphery Foci 0.001150 0.003160 0.000373 0.001172 0.001067 0.002074 0.000349 0.000763 0.001092 0.002197 0.000365 0.001167
Nucleolus 0.007111 0.008041 0.003040 0.001687 0.005264 0.013837 0.002357 0.005006 0.005823 0.013183 0.002813 0.001731
Nucleus 0.526568* 0.643882* 0.946863* 0.762833* 0.635452* 0.491501* 0.797250* 0.724529* 0.602538* 0.508691* 0.897196* 0.762328*
Peroxisomes 0.004632 0.022386 0.000142 0.000323 0.002925 0.021274 0.000462 0.000328 0.003441 0.021399 0.000248 0.000323
Vacuole 0.006067 0.023580 0.004902 0.023387 0.005418 0.013203 0.006309 0.053471 0.005615 0.014374 0.005369 0.023783
Vacuole Periphery 0.000434 0.000718 0.000133 0.003180 0.000388 0.001395 0.000089 0.000156 0.000402 0.001319 0.000118 0.003140

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.77 -13.82 -12.81 1.23 -2.35 -10.51 -6.56 -3.41 -1.35 -1.78 -11.21 -15.08 -12.85 -4.32 -4.00
Bud Neck -2.40 -3.64 -4.54 -0.16 -0.53 -7.49 -0.89 -0.45 3.69 0.01 -7.00 -5.30 -7.71 -1.61 -2.44
Bud Site -3.00 -3.55 -7.22 0.44 -4.73 -6.07 -1.75 -2.60 3.00 -0.58 -6.74 -3.86 -7.25 -0.65 -5.00
Cell Periphery -2.08 5.22 -0.48 1.62 -3.90 -2.77 -5.46 -1.26 -0.02 1.74 -2.72 -4.77 -3.08 2.07 3.37
Cytoplasm 10.60 32.41 22.63 3.45 -14.60 5.80 15.36 4.08 2.73 -0.14 8.97 40.12 31.08 18.72 -6.01
Cytoplasmic Foci 3.32 21.80 21.58 7.17 -5.51 -11.38 16.64 5.28 11.11 -0.63 -7.68 28.61 29.72 28.54 3.56
Eisosomes -1.98 10.35 6.97 5.53 -9.00 -15.39 -2.11 -3.09 -0.13 1.83 -11.59 -1.79 3.16 14.36 1.90
Endoplasmic Reticulum -8.00 -17.51 -22.24 -4.36 -6.96 -7.11 -7.48 -3.45 -2.96 -2.41 -9.90 -18.51 -22.35 -15.95 -9.24
Endosome -2.35 7.45 0.51 2.42 -4.83 -7.70 4.41 -0.74 4.41 -2.22 -8.02 8.19 0.30 7.60 -4.46
Golgi -1.62 2.53 -0.08 1.59 -1.92 -5.47 2.93 3.89 6.05 0.74 -5.59 4.27 -0.60 4.65 -1.86
Lipid Particles 3.31 9.89 9.60 5.57 -6.94 -10.00 11.44 3.34 10.38 -1.44 -6.23 14.75 14.43 16.12 -2.38
Mitochondria -0.01 3.17 2.66 1.64 -6.28 -9.41 1.23 3.96 10.90 0.74 -8.71 3.87 3.39 10.46 -1.73
Mitotic Spindle -2.68 -1.81 -8.38 -2.01 -7.40 -6.25 1.35 -0.98 0.12 -1.05 -6.88 -1.32 -8.42 -3.92 -7.92
None 4.70 8.12 5.55 -0.23 -3.94 -5.29 -2.33 -0.65 -0.31 -0.44 -3.40 4.72 4.63 6.26 0.23
Nuclear Periphery -1.56 1.09 -8.64 -7.44 -13.73 -1.43 2.98 -0.55 0.54 -2.07 -1.84 3.06 -8.65 -4.01 -14.31
Nuclear Periphery Foci -1.68 1.59 -0.19 1.66 -2.27 -3.00 2.15 -0.81 0.14 -1.26 -3.42 2.28 -0.56 3.22 -3.02
Nucleolus 0.25 4.21 5.24 3.09 2.83 -7.13 5.19 2.28 6.63 0.20 -6.16 5.97 7.47 10.92 2.27
Nucleus -4.39 -37.90 -20.65 -5.99 19.08 13.27 -16.75 -0.13 -2.06 2.49 9.43 -42.79 -19.73 -25.38 14.35
Peroxisomes -3.00 8.35 8.27 3.95 -1.53 -11.78 5.63 5.80 14.39 0.65 -11.86 9.16 9.24 14.81 0.68
Vacuole -5.52 -1.05 -13.56 -2.83 -12.71 -7.72 -3.92 -2.86 -2.71 -2.69 -9.46 -3.62 -13.90 -10.26 -12.19
Vacuole Periphery -2.06 3.76 -1.63 -0.94 -2.24 -5.95 4.22 2.52 7.61 -2.03 -6.25 5.05 -1.68 0.25 -2.27
Standard name
Human Ortholog
Description Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (69%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Yrr1

Yrr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yrr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available