Standard name
Human Ortholog
Description Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.42 0.44 0.52 0.43 0.45 0.18 0.23 0.21 0.14 – 0.14 0.15 0.44 0.34 0.29 0.39 0.43 0.39 0.31 0.26 0.24 0.32 0.48 0.48
Bud 0.16 0.07 0.18 0.14 0.16 0.07 0.05 0.07 0.09 – 0 0.08 0.14 0.1 0.07 0.16 0.15 0.12 0 0 0 0.09 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 – – – – – –
Cell Periphery 0 0.12 0.06 0.05 0.13 0.07 0.09 0.09 0 – 0 0.05 0.16 0.23 0.23 0.14 0.13 0.08 0 0 0.07 0.1 0.13 0.12
Cytoplasm 0.07 0.07 0 0.08 0.12 0.15 0.2 0.19 0.18 – 0.16 0.16 0.12 0.1 0.13 0.15 0.07 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0.17 0.37 0.21 0.19 0.29 0.46 0.45 0.44 0.29 – 0.24 0.29 0.36 0.46 0.48 0.05 0 0 0.4 0.45 0.36 0.21 0.19 0.17
Endosome 0 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.18 0 0.08 0.06 0 0 0 0 – 0 0 0 0.05 0 0.17 0.22 0.2 0 0.05 0 0 0 0
Mitochondria 0.42 0.07 0.22 0.62 0.12 0.33 0.31 0.15 0.56 – 0.54 0.5 0.3 0.07 0.1 0.08 0.07 0.09 0.05 0 0.11 0.05 0.07 0.06
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.14 0.14 0.14 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.1 0.12 0 0.09 0.05 0.06 0.11 0.06 – 0.08 0.08 0 0 0 0.14 0.17 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 56 72 35 16 57 19 40 31 20 – 13 16 52 44 44 131 126 77 59 61 27 13 77 75
Bud 21 11 12 5 20 8 8 11 13 – 3 8 16 13 11 54 44 24 8 9 3 3 2 1
Bud Neck 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 5 0 7 8 4 2 3 2
Bud Site 2 1 1 0 0 1 1 1 0 – 0 0 0 1 0 3 3 10 – – – – – –
Cell Periphery 4 19 4 2 17 7 15 13 6 – 1 5 19 30 34 47 39 15 2 9 7 4 21 18
Cytoplasm 10 11 3 3 15 16 34 28 26 – 15 17 14 13 20 52 21 29 1 1 0 0 0 0
Endoplasmic Reticulum 23 61 14 7 36 49 78 66 42 – 22 30 42 59 72 16 10 8 77 105 41 9 30 27
Endosome 5 2 4 0 4 2 7 4 1 – 1 0 4 2 0 10 8 5 6 6 4 0 2 5
Golgi 6 30 1 3 7 0 0 0 0 – 0 0 5 6 4 57 63 39 3 10 3 0 3 6
Mitochondria 56 12 15 23 15 35 54 23 80 – 50 53 35 9 15 28 21 17 8 6 12 2 11 8
Nucleus 0 2 3 0 0 0 2 3 1 – 1 0 0 0 2 4 1 3 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 1 0 0 2 – 1 2 0 0 1 0 0 0 0 0 2 0 0 0
Nucleolus 4 3 1 0 0 1 0 0 0 – 2 0 1 0 0 10 10 4 0 0 0 0 0 0
Peroxisomes 3 2 2 0 2 0 0 0 0 – 0 0 6 5 3 6 9 6 2 0 3 0 4 5
SpindlePole 6 0 1 0 5 0 0 0 0 – 1 1 1 1 1 49 42 27 6 1 1 1 1 0
Vac/Vac Membrane 12 17 8 0 11 5 10 16 8 – 7 8 0 1 3 49 50 30 6 10 2 0 1 1
Unique Cell Count 134 165 67 37 126 107 174 150 144 92 105 118 128 150 340 291 196 192 236 116 43 163 158
Labelled Cell Count 208 243 105 59 189 144 249 197 199 117 140 195 184 210 516 452 294 192 236 116 43 163 158


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 12.8 8.1 7.9 9.2 7.2 7.4 7.4 6.5 – 6.0 6.4 10.0 10.2 10.1 13.2 14.1 13.1 – 9.5 10.2
Std Deviation (1e-4) 2.3 2.6 1.7 1.2 1.3 1.2 1.3 1.1 1.1 – 1.0 1.2 1.6 2.0 2.0 3.0 3.0 3.3 – 1.5 1.9
Intensity Change (Log2) – – – -0.05 0.19 -0.18 -0.13 -0.13 -0.33 – -0.44 -0.34 0.3 0.33 0.32 0.7 0.8 0.7 – 0.23 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.9 -0.9 -4.8 -4.4 -4.7 -5.9 – -5.2 -5.2 -1.1 -2.4 -3.2 -2.1 -1.3 -1.9
Bud -0.6 -0.4 -2.1 -3.4 -2.3 -1.9 – -3.1 -2.1 -0.8 -1.5 -2.3 -0.4 -0.6 -1.2
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 1.6 0 0.7 0.7 0 – 0 0 2.0 3.1 3.0 1.8 1.7 0
Cytoplasm 0 1.7 2.2 2.9 2.8 2.7 – 2.3 2.3 1.7 1.4 2.0 2.4 0 2.2
Endoplasmic Reticulum -0.2 1.2 3.3 3.4 3.3 1.3 – 0.4 1.1 2.1 3.5 3.8 0 0 -4.3
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 3.3 3.9 3.6
Mitochondria 4.0 -1.9 1.5 1.3 -1.3 4.5 – 4.0 3.7 1.1 -3.1 -2.4 -3.4 -3.7 -3.0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 2.9 2.9 2.8
Vacuole 0 -0.7 -1.8 -1.6 -0.3 -1.6 – -0.9 -1.0 0 0 0 0.5 1.0 0.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.7705 6.0946 5.9176 6.2924 5.9857 6.2256 7.9191 8.356 7.9895 7.7378 8.19 8.2358 7.6999 7.4976 7.3234 7.0956 7.0198 7.6572
Actin 0.1954 0.1421 0.2691 0.1418 0.1785 0.1622 0.1802 0.1201 0.2187 0.0634 0.1284 0.1238 0.1632 0.1485 0.178 0.1037 0.104 0.0994
Bud 0.006 0.0052 0.0255 0.0048 0.0033 0.003 0.0037 0.0068 0.0289 0.0139 0.0019 0.0023 0.0087 0.0074 0.0335 0.0072 0.0042 0.0021
Bud Neck 0.0145 0.0159 0.0164 0.05 0.011 0.1191 0.0153 0.0144 0.0149 0.0392 0.0255 0.0969 0.0132 0.0124 0.0115 0.0573 0.0435 0.0983
Bud Periphery 0.0294 0.0149 0.1237 0.0373 0.0128 0.0174 0.0124 0.0194 0.1188 0.0544 0.0118 0.0152 0.0335 0.0185 0.1419 0.0458 0.0324 0.0141
Bud Site 0.0237 0.1686 0.042 0.0051 0.0102 0.0053 0.0294 0.1464 0.0699 0.0232 0.0071 0.0029 0.0283 0.1528 0.0775 0.0053 0.0039 0.0045
Cell Periphery 0.0712 0.0764 0.0779 0.0846 0.036 0.0659 0.0732 0.0935 0.1022 0.1538 0.0809 0.0847 0.0766 0.083 0.0973 0.0903 0.1023 0.0815
Cytoplasm 0.042 0.0662 0.0287 0.0497 0.0331 0.0267 0.0552 0.0614 0.0289 0.0636 0.0318 0.0443 0.0476 0.0949 0.0308 0.0352 0.042 0.0431
Cytoplasmic Foci 0.0269 0.0153 0.0139 0.0142 0.0112 0.0191 0.0243 0.0202 0.0116 0.0073 0.0097 0.0144 0.0299 0.0363 0.0107 0.0108 0.0198 0.0174
Eisosomes 0.0205 0.0104 0.0347 0.0196 0.0642 0.0121 0.0231 0.0243 0.0176 0.0247 0.0351 0.0127 0.0225 0.0107 0.0124 0.0341 0.0308 0.0266
Endoplasmic Reticulum 0.2546 0.2673 0.1711 0.3482 0.2107 0.277 0.3096 0.3036 0.2324 0.3495 0.3561 0.3749 0.2777 0.213 0.2192 0.4332 0.2325 0.3262
Endosome 0.0566 0.0403 0.0366 0.0535 0.058 0.0554 0.0645 0.034 0.0282 0.0661 0.0455 0.0397 0.0499 0.0414 0.0266 0.0294 0.0549 0.045
Golgi 0.0906 0.0641 0.0716 0.0934 0.189 0.1369 0.0724 0.0749 0.0485 0.0349 0.1547 0.097 0.0803 0.0681 0.0566 0.0804 0.1267 0.1159
Lipid Particles 0.0804 0.0578 0.0294 0.0304 0.0483 0.0334 0.0483 0.0373 0.0138 0.0093 0.0543 0.0257 0.0781 0.0595 0.0367 0.0156 0.0308 0.0497
Mitochondria 0.0364 0.0188 0.0367 0.029 0.088 0.0343 0.0133 0.0184 0.0344 0.0532 0.0245 0.0432 0.0381 0.0211 0.0432 0.0306 0.1102 0.0419
None 0.0115 0.0031 0.0019 0.0019 0.0023 0.0017 0.0047 0.0016 0.0034 0.0048 0.0017 0.0013 0.009 0.0021 0.0018 0.0018 0.0077 0.0019
Nuclear Periphery 0.01 0.0084 0.0054 0.0074 0.0187 0.0069 0.0337 0.0062 0.0067 0.0085 0.0105 0.0045 0.0097 0.0071 0.0059 0.0042 0.01 0.0076
Nucleolus 0.0008 0.0008 0.0004 0.0004 0.0009 0.0022 0.0009 0.0005 0.0006 0.0005 0.0004 0.0003 0.001 0.0015 0.0005 0.0004 0.0008 0.0044
Nucleus 0.0021 0.0017 0.001 0.0015 0.0029 0.0023 0.0065 0.0011 0.0017 0.0015 0.0035 0.001 0.0018 0.0024 0.0013 0.0009 0.0025 0.0019
Peroxisomes 0.006 0.001 0.0014 0.0014 0.0009 0.0033 0.0043 0.0012 0.0058 0.0005 0.0006 0.0024 0.0064 0.0018 0.0012 0.0009 0.0243 0.0016
Punctate Nuclear 0.0015 0.0011 0.0005 0.0004 0.0013 0.0017 0.0061 0.0009 0.0006 0.0003 0.0005 0.0003 0.0038 0.001 0.0006 0.0003 0.0016 0.0016
Vacuole 0.0164 0.0172 0.0097 0.0201 0.0123 0.0097 0.0136 0.012 0.0097 0.0188 0.0121 0.0091 0.0172 0.0148 0.0107 0.0103 0.011 0.0112
Vacuole Periphery 0.0036 0.0033 0.0024 0.0053 0.0062 0.0044 0.0054 0.0016 0.0026 0.0087 0.0034 0.0031 0.0031 0.0018 0.0023 0.0026 0.0042 0.0039

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 55.6199 53.7408 47.1 49.2679 50.3261 53.4602 66.638 60.7864 55.0188 56.9763
Translational Efficiency 1.1683 1.1473 1.0424 1.1602 0.9513 1.0437 0.9203 0.8372 0.9239 0.8829

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3
Localization
Cell Percentages ER (39%), peroxisome (21%), mixed (6%)
Cell Cycle Regulation Yes
peroxisome - MA (4.4e-10)
Subcompartmental Group N/A

Sey1

Sey1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sey1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available