Standard name
Description Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.42 0.44 0.52 0.43 0.45 0.18 0.23 0.21 0.14 – 0.14 0.15 0.44 0.34 0.29 0.39 0.43 0.39 0.31 0.26 0.24 0.32 0.48 0.48
Bud 0.16 0.07 0.18 0.14 0.16 0.07 0.05 0.07 0.09 – 0 0.08 0.14 0.1 0.07 0.16 0.15 0.12 0 0 0 0.09 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 – – – – – –
Cell Periphery 0 0.12 0.06 0.05 0.13 0.07 0.09 0.09 0 – 0 0.05 0.16 0.23 0.23 0.14 0.13 0.08 0 0 0.07 0.1 0.13 0.12
Cytoplasm 0.07 0.07 0 0.08 0.12 0.15 0.2 0.19 0.18 – 0.16 0.16 0.12 0.1 0.13 0.15 0.07 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0.17 0.37 0.21 0.19 0.29 0.46 0.45 0.44 0.29 – 0.24 0.29 0.36 0.46 0.48 0.05 0 0 0.4 0.45 0.36 0.21 0.19 0.17
Endosome 0 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.18 0 0.08 0.06 0 0 0 0 – 0 0 0 0.05 0 0.17 0.22 0.2 0 0.05 0 0 0 0
Mitochondria 0.42 0.07 0.22 0.62 0.12 0.33 0.31 0.15 0.56 – 0.54 0.5 0.3 0.07 0.1 0.08 0.07 0.09 0.05 0 0.11 0.05 0.07 0.06
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.14 0.14 0.14 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.1 0.12 0 0.09 0.05 0.06 0.11 0.06 – 0.08 0.08 0 0 0 0.14 0.17 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 56 72 35 16 57 19 40 31 20 – 13 16 52 44 44 131 126 77 59 61 27 13 77 75
Bud 21 11 12 5 20 8 8 11 13 – 3 8 16 13 11 54 44 24 8 9 3 3 2 1
Bud Neck 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 5 0 7 8 4 2 3 2
Bud Site 2 1 1 0 0 1 1 1 0 – 0 0 0 1 0 3 3 10 – – – – – –
Cell Periphery 4 19 4 2 17 7 15 13 6 – 1 5 19 30 34 47 39 15 2 9 7 4 21 18
Cytoplasm 10 11 3 3 15 16 34 28 26 – 15 17 14 13 20 52 21 29 1 1 0 0 0 0
Endoplasmic Reticulum 23 61 14 7 36 49 78 66 42 – 22 30 42 59 72 16 10 8 77 105 41 9 30 27
Endosome 5 2 4 0 4 2 7 4 1 – 1 0 4 2 0 10 8 5 6 6 4 0 2 5
Golgi 6 30 1 3 7 0 0 0 0 – 0 0 5 6 4 57 63 39 3 10 3 0 3 6
Mitochondria 56 12 15 23 15 35 54 23 80 – 50 53 35 9 15 28 21 17 8 6 12 2 11 8
Nucleus 0 2 3 0 0 0 2 3 1 – 1 0 0 0 2 4 1 3 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 1 0 0 2 – 1 2 0 0 1 0 0 0 0 0 2 0 0 0
Nucleolus 4 3 1 0 0 1 0 0 0 – 2 0 1 0 0 10 10 4 0 0 0 0 0 0
Peroxisomes 3 2 2 0 2 0 0 0 0 – 0 0 6 5 3 6 9 6 2 0 3 0 4 5
SpindlePole 6 0 1 0 5 0 0 0 0 – 1 1 1 1 1 49 42 27 6 1 1 1 1 0
Vac/Vac Membrane 12 17 8 0 11 5 10 16 8 – 7 8 0 1 3 49 50 30 6 10 2 0 1 1
Unique Cell Count 134 165 67 37 126 107 174 150 144 92 105 118 128 150 340 291 196 192 236 116 43 163 158
Labelled Cell Count 208 243 105 59 189 144 249 197 199 117 140 195 184 210 516 452 294 192 236 116 43 163 158


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 12.8 8.1 7.9 9.2 7.2 7.4 7.4 6.5 – 6.0 6.4 10.0 10.2 10.1 13.2 14.1 13.1 – 9.5 10.2
Std Deviation (1e-4) 2.3 2.6 1.7 1.2 1.3 1.2 1.3 1.1 1.1 – 1.0 1.2 1.6 2.0 2.0 3.0 3.0 3.3 – 1.5 1.9
Intensity Change (Log2) – – – -0.05 0.19 -0.18 -0.13 -0.13 -0.33 – -0.44 -0.34 0.3 0.33 0.32 0.7 0.8 0.7 – 0.23 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.9 -0.9 -4.8 -4.4 -4.7 -5.9 – -5.2 -5.2 -1.1 -2.4 -3.2 -2.1 -1.3 -1.9
Bud -0.6 -0.4 -2.1 -3.4 -2.3 -1.9 – -3.1 -2.1 -0.8 -1.5 -2.3 -0.4 -0.6 -1.2
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 1.6 0 0.7 0.7 0 – 0 0 2.0 3.1 3.0 1.8 1.7 0
Cytoplasm 0 1.7 2.2 2.9 2.8 2.7 – 2.3 2.3 1.7 1.4 2.0 2.4 0 2.2
Endoplasmic Reticulum -0.2 1.2 3.3 3.4 3.3 1.3 – 0.4 1.1 2.1 3.5 3.8 0 0 -4.3
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 3.3 3.9 3.6
Mitochondria 4.0 -1.9 1.5 1.3 -1.3 4.5 – 4.0 3.7 1.1 -3.1 -2.4 -3.4 -3.7 -3.0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 2.9 2.9 2.8
Vacuole 0 -0.7 -1.8 -1.6 -0.3 -1.6 – -0.9 -1.0 0 0 0 0.5 1.0 0.7