Standard name | |
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Description | Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 56 | 72 | 35 | 16 | 57 | 19 | 40 | 31 | 20 | – | 13 | 16 | 52 | 44 | 44 | 131 | 126 | 77 | 59 | 61 | 27 | 13 | 77 | 75 |
Bud | 21 | 11 | 12 | 5 | 20 | 8 | 8 | 11 | 13 | – | 3 | 8 | 16 | 13 | 11 | 54 | 44 | 24 | 8 | 9 | 3 | 3 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 7 | 8 | 4 | 2 | 3 | 2 |
Bud Site | 2 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | – | 0 | 0 | 0 | 1 | 0 | 3 | 3 | 10 | – | – | – | – | – | – |
Cell Periphery | 4 | 19 | 4 | 2 | 17 | 7 | 15 | 13 | 6 | – | 1 | 5 | 19 | 30 | 34 | 47 | 39 | 15 | 2 | 9 | 7 | 4 | 21 | 18 |
Cytoplasm | 10 | 11 | 3 | 3 | 15 | 16 | 34 | 28 | 26 | – | 15 | 17 | 14 | 13 | 20 | 52 | 21 | 29 | 1 | 1 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 23 | 61 | 14 | 7 | 36 | 49 | 78 | 66 | 42 | – | 22 | 30 | 42 | 59 | 72 | 16 | 10 | 8 | 77 | 105 | 41 | 9 | 30 | 27 |
Endosome | 5 | 2 | 4 | 0 | 4 | 2 | 7 | 4 | 1 | – | 1 | 0 | 4 | 2 | 0 | 10 | 8 | 5 | 6 | 6 | 4 | 0 | 2 | 5 |
Golgi | 6 | 30 | 1 | 3 | 7 | 0 | 0 | 0 | 0 | – | 0 | 0 | 5 | 6 | 4 | 57 | 63 | 39 | 3 | 10 | 3 | 0 | 3 | 6 |
Mitochondria | 56 | 12 | 15 | 23 | 15 | 35 | 54 | 23 | 80 | – | 50 | 53 | 35 | 9 | 15 | 28 | 21 | 17 | 8 | 6 | 12 | 2 | 11 | 8 |
Nucleus | 0 | 2 | 3 | 0 | 0 | 0 | 2 | 3 | 1 | – | 1 | 0 | 0 | 0 | 2 | 4 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | – | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Nucleolus | 4 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | 2 | 0 | 1 | 0 | 0 | 10 | 10 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 3 | 2 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 6 | 5 | 3 | 6 | 9 | 6 | 2 | 0 | 3 | 0 | 4 | 5 |
SpindlePole | 6 | 0 | 1 | 0 | 5 | 0 | 0 | 0 | 0 | – | 1 | 1 | 1 | 1 | 1 | 49 | 42 | 27 | 6 | 1 | 1 | 1 | 1 | 0 |
Vac/Vac Membrane | 12 | 17 | 8 | 0 | 11 | 5 | 10 | 16 | 8 | – | 7 | 8 | 0 | 1 | 3 | 49 | 50 | 30 | 6 | 10 | 2 | 0 | 1 | 1 |
Unique Cell Count | 134 | 165 | 67 | 37 | 126 | 107 | 174 | 150 | 144 | 92 | 105 | 118 | 128 | 150 | 340 | 291 | 196 | 192 | 236 | 116 | 43 | 163 | 158 | |
Labelled Cell Count | 208 | 243 | 105 | 59 | 189 | 144 | 249 | 197 | 199 | 117 | 140 | 195 | 184 | 210 | 516 | 452 | 294 | 192 | 236 | 116 | 43 | 163 | 158 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.1 | 12.8 | 8.1 | 7.9 | 9.2 | 7.2 | 7.4 | 7.4 | 6.5 | – | 6.0 | 6.4 | 10.0 | 10.2 | 10.1 | 13.2 | 14.1 | 13.1 | – | 9.5 | 10.2 |
Std Deviation (1e-4) | 2.3 | 2.6 | 1.7 | 1.2 | 1.3 | 1.2 | 1.3 | 1.1 | 1.1 | – | 1.0 | 1.2 | 1.6 | 2.0 | 2.0 | 3.0 | 3.0 | 3.3 | – | 1.5 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.19 | -0.18 | -0.13 | -0.13 | -0.33 | – | -0.44 | -0.34 | 0.3 | 0.33 | 0.32 | 0.7 | 0.8 | 0.7 | – | 0.23 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.9 | -0.9 | -4.8 | -4.4 | -4.7 | -5.9 | – | -5.2 | -5.2 | -1.1 | -2.4 | -3.2 | -2.1 | -1.3 | -1.9 |
Bud | -0.6 | -0.4 | -2.1 | -3.4 | -2.3 | -1.9 | – | -3.1 | -2.1 | -0.8 | -1.5 | -2.3 | -0.4 | -0.6 | -1.2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 1.6 | 0 | 0.7 | 0.7 | 0 | – | 0 | 0 | 2.0 | 3.1 | 3.0 | 1.8 | 1.7 | 0 |
Cytoplasm | 0 | 1.7 | 2.2 | 2.9 | 2.8 | 2.7 | – | 2.3 | 2.3 | 1.7 | 1.4 | 2.0 | 2.4 | 0 | 2.2 |
Endoplasmic Reticulum | -0.2 | 1.2 | 3.3 | 3.4 | 3.3 | 1.3 | – | 0.4 | 1.1 | 2.1 | 3.5 | 3.8 | 0 | 0 | -4.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.3 | 3.9 | 3.6 |
Mitochondria | 4.0 | -1.9 | 1.5 | 1.3 | -1.3 | 4.5 | – | 4.0 | 3.7 | 1.1 | -3.1 | -2.4 | -3.4 | -3.7 | -3.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.9 | 2.9 | 2.8 |
Vacuole | 0 | -0.7 | -1.8 | -1.6 | -0.3 | -1.6 | – | -0.9 | -1.0 | 0 | 0 | 0 | 0.5 | 1.0 | 0.7 |