Standard name | |
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Human Ortholog | |
Description | Glutamine tRNA synthetase; monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Gln); N-terminal domain proposed to be involved in enzyme-tRNA interactions |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 1 | 9 | 14 | 16 | 14 | – | 6 | 18 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 3 | 12 | 4 | 8 | 24 | 29 | 34 | 35 | – | 17 | 38 | 1 | 1 | 3 | 7 | 6 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 101 | 336 | 283 | 159 | 253 | 286 | 389 | 376 | 281 | – | 163 | 223 | 81 | 181 | 289 | 373 | 350 | 328 | 105 | 331 | 286 | 75 | 200 | 219 |
Endoplasmic Reticulum | 0 | 0 | 12 | 0 | 17 | 24 | 55 | 67 | 33 | – | 16 | 46 | 0 | 3 | 5 | 15 | 10 | 21 | 1 | 1 | 3 | 10 | 42 | 24 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1 | 0 | 1 | 0 | 2 | 8 | 4 | 16 | – | 20 | 11 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleus | 0 | 0 | 2 | 0 | 2 | 0 | 3 | 5 | 1 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | 0 | 1 | 1 | 0 | 6 | 7 | 2 | – | 8 | 4 | 0 | 1 | 0 | 5 | 4 | 3 | 0 | 3 | 1 | 1 | 4 | 7 |
Unique Cell Count | 102 | 338 | 293 | 161 | 269 | 318 | 468 | 478 | 342 | 209 | 295 | 81 | 185 | 294 | 381 | 355 | 332 | 112 | 343 | 294 | 94 | 254 | 259 | |
Labelled Cell Count | 103 | 342 | 309 | 165 | 282 | 346 | 506 | 511 | 384 | 233 | 342 | 82 | 186 | 299 | 403 | 370 | 367 | 112 | 343 | 294 | 94 | 254 | 259 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 40.9 | 41.9 | 29.2 | 26.4 | 24.4 | 19.5 | 18.4 | 18.2 | 16.2 | – | 13.6 | 15.2 | 39.4 | 38.6 | 36.8 | 53.0 | 54.3 | 52.8 | 34.4 | 36.2 | 39.3 |
Std Deviation (1e-4) | 8.4 | 9.3 | 5.5 | 6.2 | 5.4 | 4.2 | 3.9 | 3.5 | 3.6 | – | 3.4 | 3.3 | 7.4 | 9.6 | 8.8 | 15.5 | 13.1 | 14.4 | 9.0 | 8.7 | 10.4 |
Intensity Change (Log2) | – | – | – | -0.15 | -0.26 | -0.58 | -0.67 | -0.69 | -0.85 | – | -1.1 | -0.94 | 0.43 | 0.4 | 0.33 | 0.86 | 0.89 | 0.85 | 0.24 | 0.31 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 3.0 | 3.2 | 3.5 | – | 0 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.9 | -0.7 | 1.8 | 1.2 | 1.7 | 2.9 | – | 1.9 | 3.8 | 0 | -2.3 | -2.4 | -1.8 | -1.9 | -0.3 |
Cytoplasm | 1.4 | -1.4 | -3.2 | -5.6 | -6.9 | -5.7 | – | -6.5 | -7.3 | 1.7 | 0.8 | 1.3 | 1.0 | 1.7 | 1.9 |
Endoplasmic Reticulum | 0 | 1.2 | 1.8 | 3.6 | 4.4 | 2.7 | – | 1.7 | 4.7 | 0 | -1.5 | -1.7 | -0.1 | -0.9 | 1.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 3.7 | – | 5.4 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 26.6756 | 35.2479 | 30.9508 | 27.2502 | 25.0742 | 35.2975 | 33.166 | 45.0687 | 39.5356 | 37.2121 | 33.9701 | 40.4409 | 29.9341 | 40.9059 | 35.5605 | 31.4241 | 33.751 | 36.8951 |
Actin | 0.0153 | 0.0003 | 0.0074 | 0.0264 | 0.0297 | 0.0011 | 0.0037 | 0.0002 | 0.0023 | 0.0016 | 0.0062 | 0.0008 | 0.0079 | 0.0004 | 0.0181 | 0.0001 | 0.0023 | 0.0007 |
Bud | 0.0011 | 0.0003 | 0.0015 | 0.001 | 0.0008 | 0.001 | 0.0009 | 0.0002 | 0.0007 | 0.001 | 0.0008 | 0.0004 | 0.0008 | 0.0002 | 0.0007 | 0.0007 | 0.0005 | 0.0003 |
Bud Neck | 0.0027 | 0.0004 | 0.0013 | 0.0006 | 0.0009 | 0.0019 | 0.0007 | 0.0003 | 0.0007 | 0.0009 | 0.0008 | 0.0013 | 0.0007 | 0.0002 | 0.0005 | 0.0009 | 0.0004 | 0.0011 |
Bud Periphery | 0.0012 | 0.0002 | 0.0026 | 0.0006 | 0.001 | 0.0011 | 0.0015 | 0.0001 | 0.0007 | 0.0014 | 0.0005 | 0.0004 | 0.0006 | 0.0001 | 0.0009 | 0.0004 | 0.0004 | 0.0002 |
Bud Site | 0.0045 | 0.0006 | 0.0025 | 0.0011 | 0.0046 | 0.0001 | 0.0018 | 0.0002 | 0.0006 | 0.0026 | 0.0127 | 0.0001 | 0.0008 | 0.0002 | 0.001 | 0.0002 | 0.0001 | 0 |
Cell Periphery | 0.0018 | 0.0016 | 0.0018 | 0.0004 | 0.0003 | 0.0008 | 0.0037 | 0.0013 | 0.0014 | 0.0009 | 0.0003 | 0.0006 | 0.0011 | 0.0005 | 0.0004 | 0.0014 | 0.0002 | 0.0003 |
Cytoplasm | 0.741 | 0.9617 | 0.827 | 0.7655 | 0.7259 | 0.8813 | 0.8267 | 0.9577 | 0.9001 | 0.8613 | 0.848 | 0.8941 | 0.7895 | 0.9761 | 0.9079 | 0.8473 | 0.8615 | 0.9003 |
Cytoplasmic Foci | 0.0102 | 0.0015 | 0.0093 | 0.0153 | 0.022 | 0.0045 | 0.0121 | 0.0012 | 0.0062 | 0.0078 | 0.0156 | 0.0068 | 0.0133 | 0.0014 | 0.0062 | 0.0026 | 0.0067 | 0.0042 |
Eisosomes | 0.0006 | 0 | 0.0002 | 0.0006 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0781 | 0.0115 | 0.022 | 0.012 | 0.0086 | 0.0675 | 0.0416 | 0.0274 | 0.015 | 0.0174 | 0.0106 | 0.0542 | 0.07 | 0.0116 | 0.0163 | 0.02 | 0.0155 | 0.0502 |
Endosome | 0.0153 | 0.0023 | 0.0104 | 0.0171 | 0.0149 | 0.0127 | 0.0194 | 0.0022 | 0.0119 | 0.0225 | 0.0291 | 0.0131 | 0.0263 | 0.0018 | 0.0103 | 0.0139 | 0.0306 | 0.013 |
Golgi | 0.0028 | 0.0002 | 0.0011 | 0.0067 | 0.0039 | 0.0011 | 0.0061 | 0.0004 | 0.0022 | 0.0028 | 0.0122 | 0.0029 | 0.0035 | 0.0003 | 0.0033 | 0.0002 | 0.005 | 0.0024 |
Lipid Particles | 0.0073 | 0.0001 | 0.0056 | 0.02 | 0.0103 | 0.0001 | 0.0052 | 0.0002 | 0.0024 | 0.0051 | 0.018 | 0.001 | 0.0037 | 0.0001 | 0.0045 | 0.0003 | 0.0014 | 0.0001 |
Mitochondria | 0.0058 | 0.0001 | 0.0104 | 0.0187 | 0.0651 | 0.0011 | 0.011 | 0.0003 | 0.0091 | 0.0099 | 0.0058 | 0.0107 | 0.0069 | 0.0002 | 0.0044 | 0.0005 | 0.0333 | 0.015 |
None | 0.0178 | 0.0001 | 0.0009 | 0.0032 | 0.0455 | 0.0003 | 0.0016 | 0.0001 | 0.0007 | 0.0021 | 0.0008 | 0.0004 | 0.0047 | 0.0001 | 0.0019 | 0.0005 | 0.0009 | 0.0003 |
Nuclear Periphery | 0.0177 | 0.0021 | 0.0067 | 0.0136 | 0.0055 | 0.0069 | 0.0105 | 0.001 | 0.0027 | 0.0026 | 0.0037 | 0.0017 | 0.0143 | 0.0008 | 0.0031 | 0.0054 | 0.0114 | 0.0019 |
Nucleolus | 0.0016 | 0 | 0.0005 | 0.0007 | 0.0034 | 0 | 0.0012 | 0 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0012 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 |
Nucleus | 0.007 | 0.0013 | 0.0035 | 0.0045 | 0.0049 | 0.0019 | 0.0071 | 0.0005 | 0.0012 | 0.0029 | 0.0014 | 0.0009 | 0.0069 | 0.0007 | 0.0014 | 0.0033 | 0.0032 | 0.0009 |
Peroxisomes | 0.0077 | 0 | 0.0051 | 0.0062 | 0.0152 | 0 | 0.0019 | 0 | 0.0017 | 0.0014 | 0.006 | 0.0001 | 0.0063 | 0 | 0.0033 | 0 | 0.0002 | 0.0001 |
Punctate Nuclear | 0.005 | 0 | 0.0016 | 0.0012 | 0.0237 | 0.0001 | 0.0028 | 0 | 0.0003 | 0.0014 | 0.0031 | 0.0002 | 0.0061 | 0 | 0.0006 | 0.0001 | 0.0012 | 0 |
Vacuole | 0.0457 | 0.0139 | 0.0662 | 0.0592 | 0.0107 | 0.0135 | 0.0347 | 0.0062 | 0.033 | 0.0455 | 0.0182 | 0.0084 | 0.0298 | 0.0046 | 0.0123 | 0.0899 | 0.0168 | 0.007 |
Vacuole Periphery | 0.0099 | 0.0016 | 0.0125 | 0.0254 | 0.0029 | 0.0032 | 0.0058 | 0.0005 | 0.0068 | 0.0084 | 0.0059 | 0.0016 | 0.0056 | 0.0004 | 0.0023 | 0.0124 | 0.0084 | 0.0019 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 182.3115 | 172.9203 | 177.4111 | 200.3632 | 189.5708 | 142.5009 | 149.5986 | 153.8773 | 150.2345 | 175.4805 |
Translational Efficiency | 1.6014 | 1.8489 | 1.7031 | 1.4568 | 1.4848 | 1.8946 | 1.5696 | 1.4447 | 1.4851 | 1.5266 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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