Standard name
Description Glutamine tRNA synthetase; monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Gln); N-terminal domain proposed to be involved in enzyme-tRNA interactions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.08 0.06 0.07 0.1 – 0.08 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.99 0.94 0.9 0.83 0.79 0.82 – 0.78 0.76 1.0 0.98 0.98 0.98 0.99 0.99 0.95 0.97 0.97 0.81 0.79 0.85
Endoplasmic Reticulum 0 0 0 0 0.06 0.08 0.12 0.14 0.1 – 0.08 0.16 0 0 0 0 0 0.06 0 0 0 0.11 0.17 0.09
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 – 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 1 9 14 16 14 – 6 18 0 0 0 0 0 0 0 0 0 0 0 2
Bud 0 0 0 0 0 0 1 0 1 – 1 0 0 0 0 1 0 0 1 2 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 12 4 8 24 29 34 35 – 17 38 1 1 3 7 6 12 0 0 0 0 0 0
Cytoplasm 101 336 283 159 253 286 389 376 281 – 163 223 81 181 289 373 350 328 105 331 286 75 200 219
Endoplasmic Reticulum 0 0 12 0 17 24 55 67 33 – 16 46 0 3 5 15 10 21 1 1 3 10 42 24
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 1 0 1 1 0
Golgi 0 0 0 0 0 1 1 2 1 – 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1 0 1 0 2 8 4 16 – 20 11 0 0 0 1 0 0 0 0 0 1 0 0
Nucleus 0 0 2 0 2 0 3 5 1 – 1 0 0 0 1 0 0 3 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 0 1 1 0 6 7 2 – 8 4 0 1 0 5 4 3 0 3 1 1 4 7
Unique Cell Count 102 338 293 161 269 318 468 478 342 209 295 81 185 294 381 355 332 112 343 294 94 254 259
Labelled Cell Count 103 342 309 165 282 346 506 511 384 233 342 82 186 299 403 370 367 112 343 294 94 254 259


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 40.9 41.9 29.2 26.4 24.4 19.5 18.4 18.2 16.2 – 13.6 15.2 39.4 38.6 36.8 53.0 54.3 52.8 34.4 36.2 39.3
Std Deviation (1e-4) 8.4 9.3 5.5 6.2 5.4 4.2 3.9 3.5 3.6 – 3.4 3.3 7.4 9.6 8.8 15.5 13.1 14.4 9.0 8.7 10.4
Intensity Change (Log2) – – – -0.15 -0.26 -0.58 -0.67 -0.69 -0.85 – -1.1 -0.94 0.43 0.4 0.33 0.86 0.89 0.85 0.24 0.31 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 3.0 3.2 3.5 – 0 4.3 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery -0.9 -0.7 1.8 1.2 1.7 2.9 – 1.9 3.8 0 -2.3 -2.4 -1.8 -1.9 -0.3
Cytoplasm 1.4 -1.4 -3.2 -5.6 -6.9 -5.7 – -6.5 -7.3 1.7 0.8 1.3 1.0 1.7 1.9
Endoplasmic Reticulum 0 1.2 1.8 3.6 4.4 2.7 – 1.7 4.7 0 -1.5 -1.7 -0.1 -0.9 1.2
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.7 – 5.4 3.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0