Standard name
Description m(7)GpppX pyrophosphatase regulator; non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance; DCS2 has a paralog, DCS1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.84 0.97 0.97 0.95 0.96 0.87 0.78 0.73 0.62 0.67 0.54 0.64 0.99 1.0 0.98 0.95 0.9 0.81 0.9 0.81 0.95 0.95 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.25 0 0 0.06 0.05 0.25 0.37 0.3 0.41 0.39 0.49 0.35 0 0 0 0.05 0 0.1 0 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.1 0.08 0.1 0.14 0.13 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 1 2 0 0 0
Bud 0 2 1 3 1 4 19 10 10 11 9 12 0 0 0 1 0 0 0 1 0 2 2
Bud Neck 0 0 1 0 1 1 2 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 2 2 3 3 3 0 0 0 0 0
Cell Periphery 0 0 1 1 1 1 0 5 1 2 3 4 0 0 3 2 2 0 0 1 0 0 0
Cytoplasm 112 131 193 264 365 317 481 457 160 264 129 233 228 300 353 35 37 119 133 161 40 231 231
Endoplasmic Reticulum 1 0 1 2 0 0 1 1 0 1 1 2 0 1 3 1 0 1 0 2 0 0 0
Endosome 0 0 1 2 1 6 1 2 3 1 0 0 2 0 2 1 1 2 0 2 0 1 1
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Mitochondria 33 3 1 16 18 91 229 187 105 154 118 128 0 0 0 2 0 14 3 16 0 0 2
Nucleus 0 2 0 0 1 4 14 15 14 21 7 12 0 1 1 0 0 0 0 0 0 1 1
Nuclear Periphery 1 1 0 0 0 0 6 15 6 3 7 11 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 2 3 6 6 3 4 1 3 0 0 0 0 1 1 0 2 0 1 2
Vac/Vac Membrane 0 1 1 3 4 8 25 63 21 40 33 47 0 1 1 1 2 3 2 1 0 1 2
Unique Cell Count 133 135 199 277 379 364 620 624 259 395 239 364 230 300 359 37 41 148 148 199 42 243 251
Labelled Cell Count 147 140 202 294 394 435 784 764 325 506 311 457 230 303 363 43 45 148 148 199 42 243 251


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 3.9 4.3 4.1 4.2 3.7 3.7 4.1 4.6 5.0 5.5 5.9 5.5 6.2 5.4 6.3 7.1 5.2 6.0 6.3
Std Deviation (1e-4) 0.4 0.9 1.4 2.1 2.2 2.1 1.8 2.2 3.0 3.1 3.7 3.9 1.2 9.0 1.3 1.5 2.9 0.5 1.5 1.6
Intensity Change (Log2) -0.07 -0.03 -0.22 -0.2 -0.05 0.11 0.21 0.36 0.47 0.35 0.53 0.34 0.55 0.72 0.27 0.48 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dcs2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available