Standard name | |
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Description | Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 |
Bud | 0 | 0 | 0 | 0 | 1 | 2 | 1 | – | 3 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 5 | 2 | 1 | 2 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 5 | 1 | – | 3 | 5 | 10 | 13 | 0 | 3 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 15 | 9 | 8 | 12 | 18 | 14 | 13 | – | 8 | 14 | 10 | 9 | 57 | 130 | 152 | 8 | 4 | 4 | 0 | 0 | 1 | 1 | 2 | 3 |
Endoplasmic Reticulum | 1 | 4 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 3 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 2 |
Endosome | 2 | 1 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 | 0 | 1 | 1 | 7 | 10 | 1 | 0 | 0 | 4 | 4 | 0 | 3 | 6 | 4 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 3 | 0 | 2 | 2 | 4 |
Mitochondria | 5 | 25 | 1 | 0 | 0 | 64 | 5 | – | 50 | 37 | 71 | 46 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 7 | 2 | 3 | 10 | 4 |
Nucleus | 206 | 848 | 193 | 269 | 295 | 282 | 300 | – | 297 | 349 | 361 | 416 | 733 | 935 | 951 | 83 | 53 | 62 | 215 | 863 | 195 | 439 | 642 | 625 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 1 | 5 | – | 2 | 0 | 2 | 4 | 1 | 3 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Nucleolus | 13 | 13 | 4 | 4 | 6 | 25 | 15 | – | 54 | 53 | 93 | 85 | 10 | 12 | 25 | 2 | 0 | 0 | 3 | 4 | 3 | 23 | 57 | 72 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 10 | 13 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 0 | 1 | 2 | 2 | – | 0 | 1 | 3 | 2 | 3 | 19 | 23 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 3 | 8 |
Vac/Vac Membrane | 4 | 14 | 1 | 1 | 5 | 2 | 5 | – | 0 | 4 | 5 | 7 | 1 | 3 | 15 | 5 | 6 | 7 | 4 | 23 | 5 | 39 | 88 | 106 |
Unique Cell Count | 234 | 888 | 205 | 280 | 321 | 332 | 329 | 350 | 410 | 455 | 503 | 796 | 1101 | 1150 | 100 | 63 | 73 | 245 | 921 | 217 | 518 | 826 | 851 | |
Labelled Cell Count | 249 | 914 | 208 | 286 | 329 | 398 | 348 | 418 | 465 | 555 | 587 | 809 | 1124 | 1196 | 105 | 63 | 74 | 245 | 921 | 217 | 518 | 826 | 851 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 8.8 | 7.7 | 7.9 | 7.7 | 8.3 | 5.5 | 7.0 | – | 6.0 | 6.6 | 6.1 | 6.6 | 8.8 | 8.7 | 9.1 | 10.5 | 11.6 | 10.7 | 7.8 | 8.0 | 8.1 |
Std Deviation (1e-4) | 2.8 | 1.5 | 1.3 | 1.3 | 1.8 | 1.7 | 1.6 | – | 1.2 | 1.4 | 1.3 | 1.6 | 1.9 | 2.2 | 2.4 | 3.0 | 3.2 | 2.9 | 1.4 | 1.6 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.07 | -0.53 | -0.18 | – | -0.41 | -0.25 | -0.36 | -0.25 | 0.16 | 0.15 | 0.2 | 0.41 | 0.56 | 0.44 | -0.01 | 0.02 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | 0.9 | 0.2 | 0 | – | -1.1 | -0.3 | -1.2 | 0 | 1.7 | 3.4 | 3.8 | 1.5 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 6.5 | 0 | – | 5.4 | 4.1 | 5.8 | 4.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.0 | -1.0 | -3.2 | -1.2 | – | -3.3 | -3.3 | -4.8 | -4.0 | -1.0 | -3.5 | -4.2 | -3.1 | -2.5 | -2.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 2.8 | 1.6 | – | 5.0 | 4.4 | 6.2 | 5.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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