Standard name
Description Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0 0 0 0.06 0 0 – 0 0 0 0 0.07 0.12 0.13 0.08 0.06 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.19 0 – 0.14 0.09 0.16 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.88 0.95 0.94 0.96 0.92 0.85 0.91 – 0.85 0.85 0.79 0.83 0.92 0.85 0.83 0.83 0.84 0.85 0.88 0.94 0.9 0.85 0.78 0.73
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0.08 0.05 – 0.15 0.13 0.2 0.17 0 0 0 0 0 0 0 0 0 0 0.07 0.09
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.1 0.1 0 0 0 0.08 0.11 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 1 1 0 0 2 1
Bud 0 0 0 0 1 2 1 – 3 1 0 1 0 0 1 0 0 0 4 5 2 1 2 7
Bud Neck 0 0 0 0 0 1 0 – 1 1 0 3 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 5 1 – 3 5 10 13 0 3 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 15 9 8 12 18 14 13 – 8 14 10 9 57 130 152 8 4 4 0 0 1 1 2 3
Endoplasmic Reticulum 1 4 0 0 0 0 0 – 0 0 0 0 2 2 1 3 0 1 0 2 0 0 1 2
Endosome 2 1 0 0 1 0 1 – 0 0 0 1 1 7 10 1 0 0 4 4 0 3 6 4
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 1 0 0 3 3 0 2 2 4
Mitochondria 5 25 1 0 0 64 5 – 50 37 71 46 0 0 0 1 0 0 2 7 2 3 10 4
Nucleus 206 848 193 269 295 282 300 – 297 349 361 416 733 935 951 83 53 62 215 863 195 439 642 625
Nuclear Periphery 1 0 0 0 0 1 5 – 2 0 2 4 1 3 3 1 0 0 0 0 0 0 2 2
Nucleolus 13 13 4 4 6 25 15 – 54 53 93 85 10 12 25 2 0 0 3 4 3 23 57 72
Peroxisomes 1 0 0 0 0 0 0 – 0 0 0 0 1 10 13 0 0 0 1 0 0 0 0 0
SpindlePole 1 0 1 0 1 2 2 – 0 1 3 2 3 19 23 0 0 0 1 2 0 2 3 8
Vac/Vac Membrane 4 14 1 1 5 2 5 – 0 4 5 7 1 3 15 5 6 7 4 23 5 39 88 106
Unique Cell Count 234 888 205 280 321 332 329 350 410 455 503 796 1101 1150 100 63 73 245 921 217 518 826 851
Labelled Cell Count 249 914 208 286 329 398 348 418 465 555 587 809 1124 1196 105 63 74 245 921 217 518 826 851


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.8 7.7 7.9 7.7 8.3 5.5 7.0 – 6.0 6.6 6.1 6.6 8.8 8.7 9.1 10.5 11.6 10.7 7.8 8.0 8.1
Std Deviation (1e-4) 2.8 1.5 1.3 1.3 1.8 1.7 1.6 – 1.2 1.4 1.3 1.6 1.9 2.2 2.4 3.0 3.2 2.9 1.4 1.6 1.7
Intensity Change (Log2) – – – -0.03 0.07 -0.53 -0.18 – -0.41 -0.25 -0.36 -0.25 0.16 0.15 0.2 0.41 0.56 0.44 -0.01 0.02 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.9 0.2 0 – -1.1 -0.3 -1.2 0 1.7 3.4 3.8 1.5 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.5 0 – 5.4 4.1 5.8 4.2 0 0 0 0 0 0
Nucleus 1.0 -1.0 -3.2 -1.2 – -3.3 -3.3 -4.8 -4.0 -1.0 -3.5 -4.2 -3.1 -2.5 -2.5
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 2.8 1.6 – 5.0 4.4 6.2 5.4 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Syc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available