Standard name | |
---|---|
Human Ortholog | |
Description | Kinetochore-associated protein; required for chromosome segregation and kinetochore clustering; required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 4 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 |
Bud | 0 | 6 | 3 | 2 | 7 | 7 | 10 | 24 | 14 | – | 28 | 20 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 5 | 1 | 5 | 11 | 15 |
Bud Neck | 1 | 7 | 10 | 4 | 4 | 6 | 14 | 18 | 7 | – | 15 | 12 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 3 | 2 | 5 |
Bud Site | 1 | 10 | 14 | 13 | 13 | 20 | 18 | 14 | 33 | – | 65 | 30 | 9 | 2 | 6 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | – | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 12 | 9 | 11 | 14 | 15 | 21 | 45 | 20 | – | 19 | 18 | 14 | 39 | 36 | 18 | 15 | 11 | 0 | 2 | 0 | 1 | 2 | 5 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 3 | 1 |
Endosome | 2 | 23 | 5 | 6 | 3 | 3 | 2 | 1 | 0 | – | 0 | 0 | 31 | 94 | 33 | 38 | 55 | 39 | 7 | 41 | 31 | 14 | 16 | 20 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 7 | 1 | 0 | 2 | 2 |
Mitochondria | 3 | 56 | 14 | 7 | 7 | 26 | 47 | 32 | 62 | – | 57 | 38 | 27 | 6 | 7 | 8 | 6 | 6 | 0 | 12 | 3 | 1 | 5 | 9 |
Nucleus | 3 | 17 | 12 | 12 | 16 | 12 | 28 | 29 | 23 | – | 26 | 27 | 10 | 44 | 46 | 29 | 32 | 14 | 1 | 6 | 4 | 2 | 11 | 7 |
Nuclear Periphery | 0 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Nucleolus | 40 | 143 | 70 | 57 | 117 | 89 | 139 | 104 | 82 | – | 96 | 87 | 173 | 203 | 146 | 119 | 130 | 78 | 2 | 26 | 10 | 4 | 5 | 3 |
Peroxisomes | 2 | 11 | 4 | 1 | 1 | 4 | 2 | 4 | 2 | – | 3 | 0 | 7 | 54 | 29 | 4 | 10 | 5 | 0 | 6 | 2 | 10 | 7 | 9 |
SpindlePole | 49 | 232 | 247 | 163 | 254 | 200 | 310 | 278 | 157 | – | 232 | 209 | 164 | 234 | 154 | 109 | 130 | 70 | 73 | 258 | 234 | 149 | 270 | 178 |
Vac/Vac Membrane | 0 | 5 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | – | 0 | 0 | 0 | 4 | 1 | 6 | 6 | 4 | 0 | 4 | 3 | 1 | 8 | 11 |
Unique Cell Count | 60 | 357 | 283 | 201 | 309 | 252 | 415 | 395 | 262 | 336 | 295 | 273 | 442 | 340 | 215 | 219 | 140 | 91 | 395 | 308 | 206 | 365 | 280 | |
Labelled Cell Count | 101 | 528 | 391 | 277 | 438 | 382 | 594 | 557 | 401 | 542 | 443 | 437 | 681 | 460 | 339 | 388 | 232 | 91 | 395 | 308 | 206 | 365 | 280 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 5.1 | 5.6 | 5.3 | 6.0 | 4.7 | 4.9 | 5.3 | 4.1 | – | 3.8 | 4.4 | 5.1 | 5.4 | 5.4 | 8.9 | 9.2 | 8.8 | 4.7 | 4.8 | 4.8 |
Std Deviation (1e-4) | 1.3 | 1.0 | 1.1 | 1.4 | 1.5 | 1.2 | 1.3 | 1.8 | 1.3 | – | 1.2 | 1.3 | 1.3 | 1.3 | 1.7 | 1.9 | 2.5 | 1.9 | 1.3 | 1.7 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.1 | -0.26 | -0.19 | -0.07 | -0.45 | – | -0.55 | -0.35 | -0.12 | -0.06 | -0.06 | 0.68 | 0.72 | 0.66 | -0.24 | -0.2 | -0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1.3 | 3.3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | -1.0 | -1.8 | 0 | -0.1 | 0.7 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.7 | -0.4 | 0 | -0.4 | -0.9 | 0 | – | 0 | 0 | -1.0 | -4.0 | -2.2 | -3.3 | -3.3 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.2 | 0.8 | 1.5 | 1.2 | 3.9 | 2.3 | – | 1.5 | 1.7 | 1.2 | 3.0 | 3.6 | 2.5 | 1.9 | 2.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 4.6 | 7.5 | 4.1 | 6.3 | 8.0 | 8.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.8 | -1.8 | 0 | 2.9 | 1.6 | 0 | – | 0 | 0 | 2.2 | -2.9 | -2.0 | -0.7 | -1.3 | -0.3 |
Nucleus | 0.9 | 0.5 | 0.3 | 1.4 | 1.7 | 2.2 | – | 1.8 | 2.4 | -0.3 | 2.8 | 4.0 | 3.7 | 4.1 | 2.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.9 | 3.4 | 0 | 2.5 | 0.5 | 0 | – | 0 | 0 | 9.2 | 5.7 | 4.8 | 7.0 | 7.9 | 6.3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1.0 | 5.2 | 3.9 | 0 | 2.1 | 0 |
SpindlePole | -1.9 | -1.7 | 0 | -4.1 | -5.2 | 0 | – | 0 | 0 | -7.3 | -9.5 | -10.9 | -9.0 | -7.2 | -8.3 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.8115 | 3.7413 | 3.373 | 2.7924 | 2.8384 | 2.585 | 0.5621 | 2.1828 | 1.7958 | 1.4923 | 1.4337 | 1.1088 | -0.8091 | 0.4858 | 0.5091 | 0.7243 | 0.2324 | -0.3977 |
Actin | 0.0181 | 0 | 0.0166 | 0.0143 | 0.0002 | 0.0007 | 0.0127 | 0 | 0.0012 | 0.0001 | 0.0003 | 0.0044 | 0.0062 | 0 | 0.0002 | 0 | 0.0017 | 0.0002 |
Bud | 0.0005 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0006 | 0.0001 | 0.0011 | 0 | 0.0001 | 0.0003 |
Bud Neck | 0.0022 | 0.0003 | 0.0014 | 0.0002 | 0.0001 | 0.0045 | 0.001 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0004 | 0.0009 | 0 | 0.0016 | 0.0069 |
Bud Periphery | 0.0013 | 0 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0014 | 0.0003 | 0.0018 | 0 | 0.0003 | 0.0012 |
Bud Site | 0.0046 | 0.0001 | 0.002 | 0.0005 | 0 | 0.0001 | 0.0019 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0013 | 0.0013 | 0.0068 | 0 | 0.0005 | 0.0002 |
Cell Periphery | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0.0002 | 0.0006 | 0 | 0.0003 | 0.0011 |
Cytoplasm | 0.0017 | 0 | 0.0007 | 0.0001 | 0.0001 | 0.0004 | 0.0009 | 0 | 0.0039 | 0 | 0.0002 | 0 | 0.0053 | 0.0004 | 0.0007 | 0.0004 | 0.0004 | 0.001 |
Cytoplasmic Foci | 0.0538 | 0.0022 | 0.0159 | 0.0138 | 0.0307 | 0.0058 | 0.0377 | 0.0067 | 0.0107 | 0.0195 | 0.0297 | 0.0027 | 0.0438 | 0.0588 | 0.0139 | 0.0387 | 0.0223 | 0.0331 |
Eisosomes | 0.0012 | 0 | 0.0015 | 0.0001 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 | 0.0008 | 0.0033 | 0.0002 | 0.001 | 0 | 0.0004 | 0.0001 |
Endoplasmic Reticulum | 0.0005 | 0 | 0.0006 | 0.0001 | 0 | 0.0002 | 0.0004 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0006 | 0 |
Endosome | 0.0063 | 0.0001 | 0.0057 | 0.0009 | 0.0005 | 0.0022 | 0.0052 | 0.0002 | 0.0071 | 0.0002 | 0.0047 | 0.0007 | 0.0037 | 0.0029 | 0.0002 | 0.0001 | 0.0061 | 0.0008 |
Golgi | 0.0051 | 0.0001 | 0.0026 | 0.0013 | 0.0015 | 0.0007 | 0.0025 | 0.0003 | 0.002 | 0.0004 | 0.0035 | 0.0011 | 0.0013 | 0.0008 | 0.0002 | 0.0001 | 0.0058 | 0.0005 |
Lipid Particles | 0.0331 | 0.0012 | 0.0148 | 0.0149 | 0.0323 | 0.0077 | 0.0228 | 0.0061 | 0.0183 | 0.0052 | 0.0469 | 0.0029 | 0.011 | 0.0194 | 0.0029 | 0.0034 | 0.1739 | 0.0151 |
Mitochondria | 0.0128 | 0 | 0.0187 | 0.0067 | 0 | 0.0021 | 0.0011 | 0 | 0.0055 | 0 | 0.0008 | 0.0025 | 0.0017 | 0.0015 | 0.0011 | 0 | 0.0021 | 0.0006 |
None | 0.0388 | 0 | 0.0087 | 0.0003 | 0.0001 | 0.0088 | 0.0069 | 0 | 0.0012 | 0 | 0.0002 | 0.0001 | 0.069 | 0.0961 | 0.0589 | 0.0024 | 0.0018 | 0.1069 |
Nuclear Periphery | 0.0023 | 0.0002 | 0.0038 | 0.0006 | 0.0031 | 0.0019 | 0.0011 | 0.0024 | 0.0018 | 0.0009 | 0.0044 | 0.0021 | 0.0035 | 0.0002 | 0.0004 | 0.0002 | 0.0082 | 0.0005 |
Nucleolus | 0.0124 | 0.0572 | 0.0223 | 0.0241 | 0.0041 | 0.0158 | 0.0145 | 0.036 | 0.0142 | 0.0028 | 0.0033 | 0.0064 | 0.0131 | 0.0496 | 0.0399 | 0.0019 | 0.037 | 0.0162 |
Nucleus | 0.0166 | 0.0191 | 0.0045 | 0.0024 | 0.0005 | 0.0067 | 0.0053 | 0.0177 | 0.0043 | 0.0006 | 0.0008 | 0.0022 | 0.0229 | 0.0095 | 0.0171 | 0.0008 | 0.006 | 0.005 |
Peroxisomes | 0.0136 | 0.0001 | 0.0063 | 0.023 | 0.0105 | 0.0019 | 0.0121 | 0.0016 | 0.0033 | 0.0005 | 0.0191 | 0.0015 | 0.0042 | 0.011 | 0.0018 | 0.0003 | 0.012 | 0.0126 |
Punctate Nuclear | 0.7727 | 0.9193 | 0.8709 | 0.8963 | 0.9162 | 0.9397 | 0.8724 | 0.9276 | 0.925 | 0.9696 | 0.8855 | 0.9722 | 0.8055 | 0.7452 | 0.8503 | 0.9516 | 0.7174 | 0.7971 |
Vacuole | 0.0013 | 0 | 0.0011 | 0 | 0 | 0.0002 | 0.0008 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0006 | 0.0018 | 0.0003 | 0 | 0.001 | 0.0004 |
Vacuole Periphery | 0.0006 | 0 | 0.001 | 0.0001 | 0 | 0.0003 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0.0003 | 0 | 0 | 0.0007 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 38.9279 | 27.9993 | 6.233 | 12.0196 | 32.4875 | 32.1984 | 19.3115 | 12.5563 | 11.47 | 33.2088 |
Translational Efficiency | 2.7733 | 1.7276 | 1.9458 | 1.6981 | 1.9715 | 2.6093 | 1.6548 | 1.3601 | 1.2989 | 1.6825 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|