Standard name
Human Ortholog
Description Protein involved in biosynthesis of the coenzyme lipoic acid; has similarity to E. coli lipoic acid synthase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.08 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0.08 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.29 0.46 0.42 0.22 0.2 0.1 0 0.09 0 0.1 0.11 0.09
Mitochondria 0.96 0.94 0.94 0.96 0.9 0.94 0.93 0.9 0.93 0.92 0.94 0.94 0.85 0.65 0.71 0.07 0.07 0.09 0.86 0.79 0.83 0.74 0.7 0.71
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.07 0.06 0 0 0 0.11 0.1 0.12 0.13 0.08 0.1 0.11 0.05 0 0 0.06 0.11 0.15 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0.07 0.12 0.09 0.1 0.1 0.08 0.07 0.12 0 0 0 0.09 0.08 0.11 0 0 0 0 0 0
Peroxisomes 0 0 0.09 0 0.06 0 0 0 0 0 0 0 0 0 0 0.12 0.14 0.18 0.05 0 0.07 0 0 0.06
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.07 0 0 0.05 0.05 0 0 0 0 0 0.05 0.11 0.18 0.11 0.18 0.17 0.16 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 5 3 1 1 0 0 0 2 2 0 0 3 2 9 6 12 7 3 4 3 6 10 11
Bud 0 1 6 6 6 6 7 6 8 10 7 7 1 0 3 9 8 4 1 0 0 2 4 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 1 1 0 0 2 2 6 3 3 3 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 8 5 4 3 3 6 4 3 3 1 1 13 16 23 9 13 7 0 0 1 0 1 1
Cytoplasm 4 1 3 6 4 1 0 6 4 2 4 1 10 10 6 5 10 7 0 0 1 0 0 0
Endoplasmic Reticulum 2 3 2 0 0 0 2 1 3 0 2 0 0 5 4 2 6 1 0 0 1 0 1 1
Endosome 1 1 2 0 4 0 1 1 2 0 0 1 11 17 13 9 6 6 4 7 3 9 13 12
Golgi 1 5 7 2 1 0 0 1 0 0 0 0 135 252 228 22 27 9 6 22 7 32 45 37
Mitochondria 233 231 221 132 169 249 281 257 320 305 271 285 398 353 388 7 9 8 204 205 192 240 294 300
Nucleus 0 0 0 0 1 1 1 1 1 2 2 1 3 0 4 2 0 4 0 0 0 0 4 0
Nuclear Periphery 16 14 8 6 3 30 29 34 43 28 28 32 22 20 23 6 14 14 0 1 0 1 4 11
Nucleolus 16 7 10 2 13 32 26 29 36 28 19 36 8 8 7 9 10 10 2 1 0 2 3 2
Peroxisomes 4 3 21 3 12 6 7 6 0 10 1 1 8 11 16 12 18 17 11 11 17 14 10 26
SpindlePole 1 0 1 0 1 1 4 3 6 4 1 1 3 5 8 3 3 1 0 0 0 2 2 2
Vac/Vac Membrane 8 16 4 3 9 12 7 6 4 9 9 16 50 99 60 18 23 15 2 3 1 9 20 10
Unique Cell Count 242 245 234 138 188 266 302 285 343 332 289 304 467 544 543 101 132 93 237 261 232 327 421 424
Labelled Cell Count 289 296 293 165 229 343 377 358 435 406 346 384 665 798 792 119 159 110 237 261 232 327 421 424


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 11.2 9.9 9.3 9.2 8.0 8.1 8.2 7.5 7.9 7.4 7.5 12.0 11.5 10.7 12.7 12.3 12.6 12.2 12.7 13.6
Std Deviation (1e-4) 2.3 2.8 1.8 1.7 2.0 2.0 2.1 2.3 2.1 1.9 2.1 1.9 2.0 1.9 1.7 3.4 2.9 3.5 2.9 2.7 2.7
Intensity Change (Log2) – – – -0.1 -0.11 -0.31 -0.3 -0.28 -0.4 -0.34 -0.42 -0.41 0.27 0.22 0.1 0.35 0.31 0.35 0.3 0.36 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 3.6 0
Bud 0 0.4 -0.2 -0.2 -0.3 -0.2 0.3 -0.1 -0.2 0 0 0 0 1.7 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0.5 0.6 1.4 0 3.3 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 1.9 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 8.1 11.8 10.9 5.6 5.5 0
Mitochondria 0.5 -1.8 -0.4 -0.7 -1.8 -0.6 -1.2 -0.3 -0.3 -3.6 -8.6 -7.1 -15.8 -16.7 -15.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.5 0 3.3 2.8 3.5 3.8 2.4 2.8 3.1 0.8 0.2 0.5 0 2.8 3.8
Nucleolus 0 1.2 3.1 2.0 2.5 2.7 1.9 1.1 3.1 -2.0 -2.4 -2.6 1.7 1.3 2.2
Peroxisomes -2.6 -1.0 -3.3 -3.4 -3.5 -5.7 -3.1 -4.9 -5.0 -4.6 -4.5 -3.6 0.8 1.4 2.4
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.8 1.8 0 0 0 0.8 1.0 2.2 4.2 6.2 4.3 5.5 5.5 5.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.8535 10.9978 11.027 10.2913 9.3642 10.8189 8.129 9.8892 8.9739 7.7233 8.0372 9.1786 8.9965 9.6244 8.9294 6.9897 7.1277 8.7457
Actin 0.0055 0.0004 0.0003 0.0001 0.0002 0.0005 0.0405 0.0025 0.0028 0.0137 0.0087 0.0015 0.0145 0.0044 0.0081 0.0041 0.0688 0.0142
Bud 0.0001 0.0001 0 0 0 0 0.0005 0.0014 0.0001 0.0002 0.0007 0 0.0011 0.0002 0 0.0001 0.0002 0
Bud Neck 0.0004 0 0 0 0 0 0.0066 0.0001 0.0003 0.0001 0.0115 0 0.0006 0 0.0001 0.0001 0.0002 0.0001
Bud Periphery 0.0002 0.0002 0.0001 0 0 0 0.0009 0.0022 0.0001 0.0003 0.0008 0.0001 0.0025 0.0004 0 0.0001 0.0003 0.0001
Bud Site 0.0013 0.0002 0 0 0 0 0.0086 0.0022 0.0035 0.0006 0.0206 0 0.0026 0.0003 0.0003 0.0006 0.0008 0
Cell Periphery 0.0001 0.0001 0 0 0 0 0.0006 0.0003 0.0001 0.0001 0.0003 0 0.0003 0.0001 0 0 0.0001 0
Cytoplasm 0 0 0 0 0 0 0.0024 0.0001 0.0002 0.0058 0.0004 0 0.0018 0 0.0003 0.0002 0 0
Cytoplasmic Foci 0.0013 0.0019 0.0001 0 0.0003 0.0005 0.0085 0.0017 0.0106 0.0067 0.0114 0.0007 0.0129 0.0005 0.0047 0.0126 0.001 0.0003
Eisosomes 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0009 0.0002 0.0001 0.0003 0.0003 0.0001 0.0002 0.0002 0.0001 0.0002 0.0005 0.0002
Endoplasmic Reticulum 0 0 0.0001 0 0 0 0.008 0.0001 0.0001 0.0023 0.0003 0 0.0014 0 0.0001 0.0001 0.0001 0.0004
Endosome 0.0032 0.0034 0.0022 0.0002 0.0014 0.0014 0.0206 0.013 0.0052 0.0252 0.0072 0.0024 0.0098 0.0016 0.0021 0.0039 0.0011 0.0012
Golgi 0.01 0.0055 0.0016 0.0003 0.0212 0.0095 0.014 0.0241 0.0074 0.0065 0.0072 0.005 0.0051 0.0112 0.0019 0.004 0.0044 0.0033
Lipid Particles 0.0053 0.0017 0.0009 0.0044 0.0003 0.0024 0.0193 0.0088 0.0178 0.036 0.0068 0.0083 0.0116 0.0064 0.004 0.0174 0.0067 0.0055
Mitochondria 0.9601 0.9798 0.985 0.9931 0.9746 0.9835 0.7677 0.8945 0.9409 0.8877 0.9052 0.9678 0.9005 0.9675 0.9652 0.8217 0.9117 0.967
None 0.0001 0 0 0 0 0 0.0012 0 0 0.0008 0.0004 0 0.0013 0 0.0004 0.0005 0.0001 0.0001
Nuclear Periphery 0.0001 0 0 0 0 0 0.0317 0.001 0 0.0029 0.0004 0.0001 0.003 0 0.0002 0.0008 0.0001 0.0006
Nucleolus 0.0001 0 0 0 0 0 0.0037 0.0004 0.0001 0.0004 0.0019 0.0001 0.0002 0.0001 0.0001 0.0008 0.0001 0.0001
Nucleus 0.0001 0 0.0057 0 0 0 0.0076 0.0002 0 0.0008 0.0011 0 0.0007 0 0.0002 0.0005 0 0
Peroxisomes 0.0099 0.0039 0.0011 0.0008 0.0011 0.0011 0.0248 0.0218 0.008 0.005 0.0071 0.012 0.0201 0.0046 0.0046 0.0524 0.0027 0.0043
Punctate Nuclear 0 0 0 0 0 0 0.005 0 0.0003 0.0012 0.0063 0 0.0047 0 0.007 0.0792 0.0001 0.0001
Vacuole 0.0005 0.0005 0.0007 0.0002 0.0001 0.0001 0.0067 0.0096 0.0006 0.002 0.0009 0.0003 0.0024 0.0002 0.0001 0.0001 0.0002 0.0002
Vacuole Periphery 0.0014 0.002 0.0019 0.0007 0.0006 0.0006 0.0199 0.0157 0.0016 0.0015 0.0008 0.0013 0.0028 0.0022 0.0004 0.0006 0.0007 0.0022

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.9528 19.8068 18.8128 19.9457 21.8326 14.1724 15.0199 16.2199 22.86 18.4233
Translational Efficiency 1.136 0.9449 1.0239 1.2047 1.0595 1.1853 1.1694 1.0184 0.7382 1.0486

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
149 165 680 1668 1759 1834 226 855 1908 1999 906 2523

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 989.20 1287.49 1299.81 1255.58 1009.42 1095.14 1429.42 1412.22 1007.84 1111.02 1332.14 1308.66
Standard Deviation 163.67 212.92 205.08 269.59 191.90 228.96 206.77 241.02 189.93 233.75 213.02 270.61
Intensity Change Log 2 — 0.380227 0.393967 0.344020 — 0.117589 0.501903 0.484438 — 0.253551 0.449490 0.416646

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000823 0.004032 0.006101 0.007755 0.000920 0.003512 0.011511 0.004760 0.000912 0.003555 0.007451 0.006740
Bud Neck 0.001499 0.002254 0.003595 0.000998 0.001315 0.000635 0.011047 0.002581 0.001330 0.000769 0.005454 0.001535
Bud Site 0.006855 0.001349 0.003644 0.002295 0.000906 0.000812 0.007946 0.003713 0.001371 0.000857 0.004717 0.002775
Cell Periphery 0.000214 0.000099 0.000126 0.000701 0.000107 0.000125 0.000201 0.000268 0.000116 0.000123 0.000144 0.000554
Cytoplasm 0.000148 0.000445 0.000562 0.000305 0.000262 0.000236 0.000688 0.000515 0.000253 0.000253 0.000594 0.000376
Cytoplasmic Foci 0.006400 0.027779 0.034957 0.004607 0.012179 0.011099 0.048647 0.018959 0.011728 0.012476 0.038372 0.009471
Eisosomes 0.000114 0.000258 0.000224 0.000707 0.000159 0.000201 0.000321 0.000246 0.000155 0.000205 0.000249 0.000551
Endoplasmic Reticulum 0.000140 0.000185 0.000348 0.000374 0.000160 0.000097 0.000413 0.000802 0.000158 0.000104 0.000364 0.000519
Endosome 0.007179 0.009122 0.018307 0.002661 0.005277 0.007139 0.021488 0.012109 0.005425 0.007303 0.019101 0.005863
Golgi 0.010327 0.049554 0.038267 0.018062 0.011547 0.023274 0.034017 0.058576 0.011452 0.025443 0.037207 0.031791
Lipid Particles 0.003757 0.005469 0.012091 0.001553 0.009886 0.004669 0.013424 0.006461 0.009407 0.004735 0.012424 0.003216
Mitochondria 0.929349* 0.851958* 0.818319* 0.941818* 0.932090* 0.931695* 0.730030* 0.835524* 0.931876* 0.925113* 0.796295* 0.905797*
Mitotic Spindle 0.000295 0.004622 0.010074 0.001335 0.003360 0.000498 0.032820 0.004923 0.003121 0.000839 0.015748 0.002551
None 0.000434 0.001077 0.000914 0.001032 0.000870 0.000618 0.000774 0.001397 0.000836 0.000656 0.000879 0.001156
Nuclear Periphery 0.000058 0.000212 0.001434 0.000140 0.000332 0.000125 0.002487 0.002257 0.000310 0.000132 0.001696 0.000857
Nuclear Periphery Foci 0.003082 0.000246 0.001132 0.000084 0.000431 0.000168 0.001839 0.001341 0.000638 0.000175 0.001309 0.000510
Nucleolus 0.000291 0.000289 0.000613 0.000091 0.000489 0.000253 0.000628 0.000373 0.000474 0.000256 0.000616 0.000187
Nucleus 0.000056 0.000126 0.000115 0.000032 0.000143 0.000061 0.000237 0.000127 0.000136 0.000066 0.000146 0.000064
Peroxisomes 0.025769 0.021994 0.031069 0.007981 0.013715 0.008804 0.064402 0.013370 0.014657 0.009893 0.039384 0.009807
Vacuole 0.001362 0.011470 0.008573 0.002684 0.003197 0.003269 0.009043 0.012439 0.003053 0.003946 0.008690 0.005990
Vacuole Periphery 0.001847 0.007460 0.009534 0.004783 0.002656 0.002709 0.008037 0.019259 0.002593 0.003101 0.009161 0.009689

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.25 -4.57 -7.27 -2.23 -1.14 -3.99 -3.53 -4.28 -1.13 2.15 -4.34 -5.71 -8.32 -3.51 0.53
Bud Neck -0.76 -2.61 0.74 1.64 5.28 2.35 -5.06 -2.45 -4.31 4.33 2.02 -6.33 -0.67 -3.67 6.26
Bud Site 0.90 0.52 0.74 -1.23 1.16 0.28 -4.03 -2.71 -2.80 2.15 0.92 -3.37 -1.95 -3.50 1.95
Cell Periphery 1.20 0.94 -1.99 -2.66 -2.55 -0.43 -2.54 -2.48 -2.06 -1.03 -0.19 -1.07 -2.87 -2.80 -2.72
Cytoplasm -3.45 -5.43 -2.41 1.45 2.96 0.31 -4.03 -2.33 -2.93 1.44 -0.03 -4.19 -1.62 -2.08 3.02
Cytoplasmic Foci -3.83 -7.78 0.96 4.37 9.36 0.76 -6.10 -3.20 -3.73 4.81 -0.55 -8.96 1.89 2.41 9.92
Eisosomes -2.78 -2.58 -6.93 -5.43 -6.22 -1.41 -3.55 -3.14 -1.24 1.47 -1.82 -4.61 -7.55 -6.08 -5.64
Endoplasmic Reticulum -0.61 -2.38 -1.67 -1.38 -0.24 2.76 -4.25 -2.29 -2.51 -1.36 2.47 -3.68 -2.78 -3.22 -1.15
Endosome -0.67 -3.65 1.96 3.40 7.45 -1.80 -4.35 -4.23 -2.96 2.37 -1.92 -7.20 -0.52 1.50 7.02
Golgi -3.99 -5.95 -2.22 3.31 5.06 -5.41 -4.28 -9.30 -6.70 -3.46 -6.50 -8.14 -8.77 -2.27 1.49
Lipid Particles -0.68 -2.95 1.10 2.37 5.07 3.70 -0.89 2.01 -1.37 1.77 3.56 -1.40 4.90 2.00 4.96
Mitochondria 3.03 5.61 -0.74 -4.52 -10.51 0.08 8.57 9.29 9.33 -4.23 1.18 12.31 4.47 3.33 -9.91
Mitotic Spindle -1.89 -4.13 -1.94 1.39 3.60 3.46 -4.04 -1.02 -3.38 3.80 2.91 -4.77 0.60 -2.83 5.06
None -3.51 -6.19 -6.91 0.24 -1.42 3.92 1.23 -5.27 -8.50 -6.04 2.94 -0.64 -4.45 -7.93 -4.15
Nuclear Periphery -3.95 -6.01 -2.15 1.44 5.60 2.31 -2.26 -2.92 -3.26 0.19 2.16 -4.57 -2.30 -3.23 2.28
Nuclear Periphery Foci 0.95 0.66 1.00 2.66 4.78 2.45 -2.33 -1.60 -2.08 0.59 1.82 -1.99 0.40 -1.72 2.70
Nucleolus 0.02 -0.88 0.81 2.13 2.03 1.19 -0.67 0.63 -0.77 1.38 1.18 -0.60 2.08 0.51 2.15
Nucleus -1.12 -1.34 0.75 1.81 2.87 1.72 -1.53 0.28 -1.64 1.92 1.58 -0.21 1.76 0.12 2.99
Peroxisomes 0.32 -0.49 1.74 2.22 5.51 2.83 -4.99 0.13 -1.84 4.95 2.56 -5.88 2.58 0.05 7.22
Vacuole -2.02 -6.05 -1.94 1.76 4.92 -0.10 -3.96 -5.43 -5.31 -1.63 -1.08 -5.60 -3.71 -2.22 2.41
Vacuole Periphery -1.72 -4.82 -3.05 0.83 3.20 -0.13 -3.18 -8.12 -8.10 -4.31 -1.08 -5.70 -8.53 -7.58 -0.41
Standard name
Human Ortholog
Description Protein involved in biosynthesis of the coenzyme lipoic acid; has similarity to E. coli lipoic acid synthase
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Lip5

Lip5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lip5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available