Standard name
Description Phosphotyrosine-specific phosphatase; major role in osmolarity sensing through dephosphorylation of the Hog1p MAPK with a minor role by Ptp3p; inactivates and regulates Hog1p localization; major role in the cell wall integrity pathway through dephosphorylation of MAPK Slt2p with a minor role by Ptp3p; minor role with Msg5p in the pheromone adaptive response through dephosphorylation of MAPK Fus3p with major role by Ptp3p; co-regulates the calcium signaling pathway with Msg5p; nuclear localized

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.61 0.68 0.87 0.8 0.52 0.42 0.35 0.44 0.26 0.28 0.29 0.43 0.72 0.77 0.76 0.43 0.41 0.33 0.37 0.52 0.49 0.47 0.48 0.53
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.25 0.3 0 0.14 0.08 0.29 0.37 0.27 0.73 0.66 0.67 0.49 0.07 0 0.07 0 0 0 0.06 0 0.07 0.08 0.06 0
Nucleus 0.08 0.06 0 0.07 0.26 0.36 0.37 0.32 0.29 0.2 0.23 0.19 0 0 0 0.18 0.18 0.15 0.24 0.16 0.12 0.07 0 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.28 0.08 0.07 0 0.16 0.13 0.07 0.06 0 0.06 0.05 0.05 0.28 0.29 0.24 0.39 0.46 0.6 0.26 0.21 0.24 0.28 0.27 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 6 3
Bud 1 0 0 0 0 1 3 5 6 11 8 7 0 0 0 0 0 1 0 0 1 0 10 12
Bud Neck 0 0 6 3 3 3 8 11 0 2 5 10 0 0 0 0 0 0 0 1 1 0 6 12
Bud Site 0 0 1 0 0 0 1 5 4 7 5 5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1 0 1 0 1 1 0 1 0 1 1 0 0 0 0 0 0 0 1 0
Cytoplasm 62 96 156 117 97 119 132 136 62 76 92 124 195 302 289 51 49 13 48 94 127 70 181 181
Endoplasmic Reticulum 1 1 0 0 0 0 2 0 0 2 2 1 0 3 1 4 2 2 0 0 2 2 6 2
Endosome 0 0 1 0 1 3 0 2 0 0 0 0 3 3 0 3 6 1 2 0 2 1 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 2
Mitochondria 25 43 2 21 14 82 140 84 172 179 215 143 20 14 25 4 3 1 7 6 19 11 21 13
Nucleus 8 9 7 11 48 101 140 97 67 53 73 54 5 4 10 21 22 6 31 28 30 10 13 18
Nuclear Periphery 2 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 1 0 0 1 0 0 0 1 0 0 0 0 2 2 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
SpindlePole 0 0 1 1 1 3 2 8 1 2 0 3 0 0 0 0 0 0 1 3 4 1 9 12
Vac/Vac Membrane 28 11 13 3 30 36 27 19 8 17 15 14 77 115 91 47 55 24 33 38 62 42 102 74
Unique Cell Count 101 142 179 147 185 282 375 307 235 270 322 291 272 393 379 119 120 40 129 184 262 150 377 345
Labelled Cell Count 127 160 187 157 195 348 458 367 321 351 417 362 300 442 417 133 140 48 129 184 262 150 377 345


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.3 4.6 4.2 4.9 3.9 4.0 4.2 3.3 3.4 3.2 3.7 4.1 4.3 4.5 6.4 6.6 6.4 4.3 4.4 4.8
Std Deviation (1e-4) 0.6 0.4 0.9 1.1 1.2 0.9 0.9 1.1 0.7 1.2 0.8 1.0 0.7 0.9 1.3 1.2 1.4 1.5 1.7 1.6 1.5
Intensity Change (Log2) -0.13 0.11 -0.22 -0.2 -0.13 -0.48 -0.44 -0.49 -0.31 -0.15 -0.1 -0.03 0.48 0.52 0.49 -0.1 -0.07 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 6.8 7.8 8.0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ptp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available