Standard name
Human Ortholog
Description RNA polymerase subunit ABC14.5; common to RNA polymerases I, II, and III

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0 0 0 0 0 0 0.06 0.11 0.11 0.12 0.08 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0 0 0 0.05 0.08 0.08 0.11 0.1 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleus 0.88 0.83 0.75 0.84 0.75 0.76 0.68 0.64 0.58 0.58 0.69 0.79 0.79 0.71 0.73 0.74 0.76 0.83 0.75 0.61 0.54 0.52 0.45
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.45 0.44 0.55 0.46 0.4 0.53 0.63 0.68 0.71 0.7 0.65 0.51 0.39 0.33 0.25 0.28 0.26 0.12 0.2 0.29 0.35 0.39 0.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 0 1 0 1 1 1 0 0 1 0 0 0 2 1 2 1 1 3
Bud Neck 0 1 0 0 1 0 1 0 3 1 1 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 1 0 0 1 0 2 8 4 7 8 9 0 0 1 0 0 1
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 7 11 10 12 6 11 5 8 2 2 17 28 36 27 11 16 0 1 1 5 2 2
Endoplasmic Reticulum 0 0 2 0 0 0 0 0 0 0 0 0 1 0 2 2 1 0 0 0 0 0 0
Endosome 0 0 0 0 4 1 0 0 0 0 0 2 3 8 3 2 2 1 0 6 0 1 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 1 1 0 2 3
Mitochondria 5 4 17 6 7 8 20 20 27 33 36 0 1 0 7 9 4 0 1 2 0 1 2
Nucleus 219 178 212 177 166 210 286 162 208 177 251 230 206 223 161 104 145 210 167 182 88 126 130
Nuclear Periphery 0 0 0 0 0 2 3 4 2 0 4 0 0 1 0 1 0 0 0 1 0 0 1
Nucleolus 113 95 155 97 88 146 266 171 254 213 237 149 102 105 55 39 50 30 44 87 57 93 116
Peroxisomes 1 1 0 1 1 0 0 0 0 0 0 1 3 3 0 0 1 0 0 0 0 0 1
SpindlePole 1 0 0 0 0 1 0 0 1 0 0 2 2 10 0 0 1 0 0 2 0 1 3
Vac/Vac Membrane 0 3 0 0 2 1 1 1 0 1 1 1 0 4 3 3 5 5 4 5 7 8 19
Unique Cell Count 250 214 281 211 222 275 422 252 357 305 364 292 261 315 222 141 192 254 224 298 165 241 292
Labelled Cell Count 342 289 397 292 281 377 597 367 511 436 542 402 347 392 258 171 227 254 224 298 165 241 292


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.3 22.3 12.3 15.6 14.8 12.3 11.3 10.5 10.8 10.5 10.6 17.4 18.6 16.9 17.4 17.6 16.4 20.6 19.7 19.6
Std Deviation (1e-4) 4.5 7.6 4.2 4.9 5.3 3.4 3.4 3.7 3.1 3.0 3.5 6.0 5.8 6.1 6.4 6.0 5.8 11.4 6.1 5.4
Intensity Change (Log2) 0.34 0.27 0.0 -0.12 -0.22 -0.18 -0.23 -0.22 0.5 0.6 0.46 0.5 0.52 0.42 0.74 0.68 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 0.8 -1.2 -1.0 -1.3 -1.2 -2.7 -3.0 1.1 3.1 3.4 3.5 1.7 2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.7 -1.5 -1.8 -0.8 0.9 0.7 2.1 1.8 -4.3 -3.7 -4.4 -1.5 0.1 -2.1
Nucleus 2.3 -0.2 0.3 -2.2 -2.8 -4.5 -4.5 -1.8 0.9 1.0 -1.3 -0.7 -0.4 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.0 -3.5 -0.5 2.1 3.0 4.2 3.7 2.6 -1.0 -3.7 -5.4 -6.9 -5.3 -6.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.1808 13.6742 12.497 11.6484 10.8139 12.1314 11.1813 11.0375 10.5618 10.372 8.7697 10.3675 14.9136 14.9744 15.0087 14.9088 13.5699 13.7176
Actin 0.0276 0 0.0126 0.0324 0.0013 0.0007 0.0055 0 0.0073 0 0.0128 0.0002 0.0059 0 0.0128 0 0 0.0002
Bud 0.0012 0 0.0003 0.0001 0.0004 0.0001 0.0009 0 0.0001 0 0.0003 0.0001 0.0003 0 0.0001 0 0 0
Bud Neck 0.0014 0 0.0001 0.0001 0.0002 0.0003 0.0004 0 0.0041 0 0.0004 0.0004 0.0003 0 0.0002 0 0.0001 0.0002
Bud Periphery 0.0015 0 0.0003 0.0002 0.0005 0.0001 0.0014 0 0.0002 0 0.0012 0.0001 0.0005 0 0.0002 0 0.0001 0
Bud Site 0.0043 0 0.0021 0.0003 0.0013 0.0002 0.0028 0 0.0016 0 0.001 0.0003 0.0006 0 0.0002 0 0 0
Cell Periphery 0.0004 0 0.0001 0 0.0004 0.0001 0.0004 0 0.0001 0 0.0013 0.0001 0.0004 0 0.0001 0 0.0001 0
Cytoplasm 0.0039 0.0001 0.0021 0.0001 0.0005 0.0001 0.0047 0 0.0002 0 0.002 0.0002 0.0012 0 0.0001 0 0 0.0001
Cytoplasmic Foci 0.0116 0 0.0079 0.0027 0.0271 0.0016 0.0174 0 0.0078 0 0.0142 0.0016 0.0003 0 0.0003 0 0 0.0001
Eisosomes 0.0003 0 0.0001 0.0002 0.0001 0 0.0001 0 0 0 0.0001 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0009 0 0.0011 0.0001 0.0002 0.0002 0.0027 0 0.0001 0 0.0007 0.0001 0.0018 0 0.0003 0 0 0.0002
Endosome 0.0131 0 0.012 0.0019 0.0043 0.0058 0.0227 0 0.0033 0 0.0114 0.0036 0.0026 0 0.0012 0 0 0.0015
Golgi 0.0072 0 0.0026 0.0045 0.0025 0.0021 0.0064 0 0.0025 0 0.0096 0.0004 0.0009 0 0.0007 0 0 0.0001
Lipid Particles 0.0107 0 0.0117 0.0032 0.0242 0.0043 0.0216 0 0.0096 0 0.0349 0.0015 0.0009 0 0.0003 0 0 0.0001
Mitochondria 0.0058 0.0002 0.0126 0.0081 0.012 0.0131 0.0096 0.0002 0.0016 0 0.0299 0.0015 0.0018 0.0001 0.0007 0 0.0009 0.0003
None 0.0049 0 0.001 0.0002 0.0012 0.0001 0.0029 0 0.0003 0 0.004 0.0002 0.0005 0 0.0004 0 0 0.0001
Nuclear Periphery 0.0151 0.0004 0.0038 0.0004 0.0018 0.0002 0.0115 0.0025 0.0009 0.0002 0.0024 0.0008 0.0068 0.0002 0.0007 0 0.0002 0.002
Nucleolus 0.1245 0.0473 0.0503 0.0694 0.4483 0.2422 0.1076 0.0638 0.0457 0.0496 0.4002 0.2523 0.1233 0.0649 0.0401 0.0497 0.3604 0.2551
Nucleus 0.7408 0.9516 0.8725 0.8629 0.4057 0.7259 0.7612 0.9323 0.901 0.9501 0.4378 0.7328 0.8474 0.9345 0.9409 0.9501 0.6377 0.7392
Peroxisomes 0.0077 0 0.0035 0.0115 0.0113 0.0017 0.0043 0 0.0099 0 0.01 0.0002 0.0001 0 0.0003 0 0 0
Punctate Nuclear 0.0126 0.0001 0.002 0.0014 0.0524 0.0004 0.0095 0.0001 0.0027 0.0001 0.0045 0.0009 0.0007 0.0002 0.0003 0 0.0003 0.0004
Vacuole 0.0027 0.0001 0.0006 0.0001 0.0016 0.0004 0.0037 0.0001 0.0004 0 0.0093 0.0019 0.0023 0 0.0001 0 0.0001 0.0002
Vacuole Periphery 0.0018 0.0001 0.0009 0.0003 0.0027 0.0004 0.0026 0.0008 0.0004 0 0.0121 0.0007 0.0013 0 0.0001 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 183.9007 219.1947 154.3534 170.8383 191.3971 117.5313 158.8708 152.6364 135.8458 160.4239
Translational Efficiency 1.3882 1.2286 1.6738 1.3091 1.212 2.0386 1.5167 1.284 1.5676 1.5217

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency