Standard name
Description RNA polymerase subunit ABC14.5; common to RNA polymerases I, II, and III

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0 0 0 0 0 0 0.06 0.11 0.11 0.12 0.08 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0 0 0 0.05 0.08 0.08 0.11 0.1 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleus 0.88 0.83 0.75 0.84 0.75 0.76 0.68 0.64 0.58 0.58 0.69 0.79 0.79 0.71 0.73 0.74 0.76 0.83 0.75 0.61 0.54 0.52 0.45
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.45 0.44 0.55 0.46 0.4 0.53 0.63 0.68 0.71 0.7 0.65 0.51 0.39 0.33 0.25 0.28 0.26 0.12 0.2 0.29 0.35 0.39 0.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 0 1 0 1 1 1 0 0 1 0 0 0 2 1 2 1 1 3
Bud Neck 0 1 0 0 1 0 1 0 3 1 1 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 1 0 0 1 0 2 8 4 7 8 9 0 0 1 0 0 1
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 7 11 10 12 6 11 5 8 2 2 17 28 36 27 11 16 0 1 1 5 2 2
Endoplasmic Reticulum 0 0 2 0 0 0 0 0 0 0 0 0 1 0 2 2 1 0 0 0 0 0 0
Endosome 0 0 0 0 4 1 0 0 0 0 0 2 3 8 3 2 2 1 0 6 0 1 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 1 1 0 2 3
Mitochondria 5 4 17 6 7 8 20 20 27 33 36 0 1 0 7 9 4 0 1 2 0 1 2
Nucleus 219 178 212 177 166 210 286 162 208 177 251 230 206 223 161 104 145 210 167 182 88 126 130
Nuclear Periphery 0 0 0 0 0 2 3 4 2 0 4 0 0 1 0 1 0 0 0 1 0 0 1
Nucleolus 113 95 155 97 88 146 266 171 254 213 237 149 102 105 55 39 50 30 44 87 57 93 116
Peroxisomes 1 1 0 1 1 0 0 0 0 0 0 1 3 3 0 0 1 0 0 0 0 0 1
SpindlePole 1 0 0 0 0 1 0 0 1 0 0 2 2 10 0 0 1 0 0 2 0 1 3
Vac/Vac Membrane 0 3 0 0 2 1 1 1 0 1 1 1 0 4 3 3 5 5 4 5 7 8 19
Unique Cell Count 250 214 281 211 222 275 422 252 357 305 364 292 261 315 222 141 192 254 224 298 165 241 292
Labelled Cell Count 342 289 397 292 281 377 597 367 511 436 542 402 347 392 258 171 227 254 224 298 165 241 292


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.3 22.3 12.3 15.6 14.8 12.3 11.3 10.5 10.8 10.5 10.6 17.4 18.6 16.9 17.4 17.6 16.4 20.6 19.7 19.6
Std Deviation (1e-4) 4.5 7.6 4.2 4.9 5.3 3.4 3.4 3.7 3.1 3.0 3.5 6.0 5.8 6.1 6.4 6.0 5.8 11.4 6.1 5.4
Intensity Change (Log2) 0.34 0.27 0.0 -0.12 -0.22 -0.18 -0.23 -0.22 0.5 0.6 0.46 0.5 0.52 0.42 0.74 0.68 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 0.8 -1.2 -1.0 -1.3 -1.2 -2.7 -3.0 1.1 3.1 3.4 3.5 1.7 2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.7 -1.5 -1.8 -0.8 0.9 0.7 2.1 1.8 -4.3 -3.7 -4.4 -1.5 0.1 -2.1
Nucleus 2.3 -0.2 0.3 -2.2 -2.8 -4.5 -4.5 -1.8 0.9 1.0 -1.3 -0.7 -0.4 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.0 -3.5 -0.5 2.1 3.0 4.2 3.7 2.6 -1.0 -3.7 -5.4 -6.9 -5.3 -6.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpb8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available