Standard name | |
---|---|
Human Ortholog | |
Description | Transcriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 10 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 1 | 2 | 4 | 5 | 6 | 42 | 52 | 45 | 37 | 31 | 46 | 8 | 26 | 22 | 115 | 77 | 59 | 0 | 0 | 1 | 0 | 1 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 18 | 20 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 3 | 3 | 5 | 5 | 22 | 60 | 88 | 78 | 74 | 96 | 68 | 93 | 3 | 1 | 2 | 99 | 71 | 32 | 0 | 0 | 0 | 0 | 3 | 3 |
Nucleus | 66 | 69 | 195 | 159 | 158 | 127 | 201 | 245 | 148 | 212 | 143 | 245 | 170 | 230 | 295 | 263 | 182 | 117 | 74 | 72 | 153 | 118 | 191 | 98 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 2 |
Nucleolus | 26 | 15 | 33 | 42 | 84 | 173 | 313 | 368 | 215 | 330 | 153 | 220 | 12 | 21 | 50 | 107 | 80 | 47 | 10 | 10 | 52 | 10 | 44 | 93 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 12 |
Vac/Vac Membrane | 1 | 1 | 0 | 0 | 12 | 5 | 19 | 35 | 27 | 29 | 16 | 22 | 1 | 3 | 13 | 20 | 21 | 12 | 0 | 1 | 10 | 16 | 29 | 31 |
Unique Cell Count | 74 | 75 | 213 | 186 | 254 | 305 | 543 | 665 | 419 | 582 | 323 | 499 | 186 | 271 | 360 | 480 | 351 | 230 | 88 | 88 | 225 | 149 | 281 | 263 |
Labelled Cell Count | 97 | 89 | 235 | 210 | 281 | 371 | 666 | 789 | 513 | 710 | 412 | 628 | 195 | 284 | 395 | 625 | 456 | 275 | 88 | 88 | 225 | 149 | 281 | 263 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 48.4 | 62.7 | 50.4 | 44.6 | 50.5 | 57.2 | 59.8 | 64.5 | 67.4 | 61.1 | 59.3 | 64.3 | 60.5 | 61.9 | 65.9 | 96.5 | 95.6 | 99.8 | 53.5 | 55.6 | 62.6 |
Std Deviation (1e-4) | 17.6 | 20.1 | 17.8 | 14.1 | 16.2 | 20.4 | 24.3 | 28.5 | 30.4 | 25.2 | 25.0 | 26.2 | 24.2 | 29.7 | 30.6 | 48.0 | 48.4 | 47.1 | 21.3 | 23.0 | 23.7 |
Intensity Change (Log2) | – | – | – | -0.17 | 0.0 | 0.18 | 0.25 | 0.36 | 0.42 | 0.28 | 0.24 | 0.35 | 0.26 | 0.3 | 0.39 | 0.94 | 0.92 | 0.99 | 0.09 | 0.14 | 0.31 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 3.6 | 3.6 | 4.4 | 3.1 | 4.1 | 4.0 | 0 | 4.0 | 3.0 | 7.5 | 7.0 | 7.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 3.5 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 2.9 | 5.9 | 5.2 | 4.1 | 5.5 | 5.3 | 6.2 | 5.8 | 0 | 0 | 0 | 6.2 | 6.0 | 4.4 |
Nucleus | -1.9 | -7.4 | -11.5 | -13.5 | -13.9 | -13.4 | -13.8 | -11.1 | -10.7 | -0.1 | -2.2 | -3.2 | -9.4 | -9.7 | -9.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.8 | 4.4 | 9.4 | 10.5 | 10.2 | 8.7 | 10.3 | 7.6 | 7.3 | -2.8 | -2.7 | -0.5 | 2.1 | 2.1 | 1.4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.2 | 0 | 2.8 | 3.4 | 3.8 | 3.3 | 3.3 | 3.1 | 0 | 0 | 0 | 3.0 | 3.6 | 3.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 37.4471 | 39.975 | 36.2402 | 33.4635 | 29.7691 | 38.1988 | 48.3537 | 44.0136 | 35.3774 | 32.8182 | 26.7102 | 38.8294 | 52.0014 | 51.4676 | 42.473 | 38.1871 | 43.7437 | 45.2047 |
Actin | 0.0188 | 0 | 0.0103 | 0.0002 | 0.0144 | 0.0003 | 0.0277 | 0 | 0.0107 | 0.0351 | 0.023 | 0.0069 | 0.001 | 0 | 0.0002 | 0.0003 | 0 | 0 |
Bud | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0004 | 0.0008 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0005 | 0 | 0.0001 |
Bud Neck | 0.0059 | 0 | 0.0005 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0 | 0.0008 | 0.0004 | 0.0003 | 0.0002 | 0.0003 | 0 | 0 | 0.0002 | 0 | 0.0002 |
Bud Periphery | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0006 | 0.0002 | 0.0002 | 0 | 0.0004 | 0.0014 | 0.0004 | 0.0004 | 0.0001 | 0 | 0 | 0.0005 | 0.0001 | 0.0002 |
Bud Site | 0.0041 | 0.0006 | 0.0076 | 0.0002 | 0.0007 | 0.0001 | 0.0004 | 0 | 0.0034 | 0.0019 | 0.0007 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0005 | 0 | 0 |
Cell Periphery | 0.0007 | 0.0001 | 0.0002 | 0.0004 | 0.0019 | 0.0005 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0008 | 0.0006 | 0.0003 | 0 | 0.0001 | 0.0008 | 0.0002 | 0.0005 |
Cytoplasm | 0.0044 | 0.0001 | 0.0003 | 0.0004 | 0.0015 | 0.0001 | 0.0017 | 0.0001 | 0.0004 | 0.0005 | 0.002 | 0.0006 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0 | 0.0001 |
Cytoplasmic Foci | 0.0161 | 0.0016 | 0.0026 | 0.0051 | 0.0061 | 0.0066 | 0.0067 | 0.0044 | 0.0158 | 0.0016 | 0.0134 | 0.021 | 0.0106 | 0.0239 | 0.0035 | 0.0255 | 0 | 0.0044 |
Eisosomes | 0.0005 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0017 | 0 | 0.0003 | 0.0002 | 0.0031 | 0 | 0.0007 | 0 | 0.0002 | 0.0006 | 0.0008 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0162 | 0 | 0.002 | 0.0006 | 0.0386 | 0.0003 | 0.0045 | 0 | 0.0022 | 0.0051 | 0.0092 | 0.0029 | 0.005 | 0 | 0.0002 | 0.0001 | 0 | 0 |
Golgi | 0.0036 | 0 | 0.0011 | 0.0002 | 0.0077 | 0.0002 | 0.0036 | 0 | 0.0035 | 0.0038 | 0.0112 | 0.0033 | 0.0014 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Lipid Particles | 0.0122 | 0.0001 | 0.0029 | 0.0053 | 0.0136 | 0.0029 | 0.0017 | 0.0002 | 0.0075 | 0.0016 | 0.0226 | 0.012 | 0.0032 | 0.0003 | 0.0033 | 0.0047 | 0 | 0.0004 |
Mitochondria | 0.0097 | 0.0024 | 0.0044 | 0.0063 | 0.0206 | 0.0084 | 0.0135 | 0.0116 | 0.0119 | 0.0064 | 0.0463 | 0.0047 | 0.0071 | 0.0014 | 0.0035 | 0.0069 | 0.0041 | 0.0011 |
None | 0.0183 | 0 | 0.0006 | 0.0014 | 0.0013 | 0.0001 | 0.0012 | 0.0001 | 0.0006 | 0.0003 | 0.0014 | 0.001 | 0.0006 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 |
Nuclear Periphery | 0.0188 | 0.0099 | 0.019 | 0.0113 | 0.0115 | 0.0028 | 0.0096 | 0.0026 | 0.0164 | 0.0294 | 0.0587 | 0.0167 | 0.0024 | 0.0051 | 0.0209 | 0.0093 | 0.0007 | 0.0012 |
Nucleolus | 0.1301 | 0.0545 | 0.0484 | 0.0951 | 0.3104 | 0.1873 | 0.0895 | 0.0527 | 0.0638 | 0.05 | 0.2646 | 0.1672 | 0.109 | 0.0386 | 0.0427 | 0.0639 | 0.2805 | 0.1831 |
Nucleus | 0.7061 | 0.9291 | 0.8947 | 0.8388 | 0.541 | 0.7803 | 0.8275 | 0.892 | 0.8509 | 0.8309 | 0.5019 | 0.7463 | 0.8508 | 0.9018 | 0.9211 | 0.8795 | 0.7122 | 0.795 |
Peroxisomes | 0.003 | 0.0001 | 0.0011 | 0.0003 | 0.0018 | 0.0003 | 0.0013 | 0 | 0.0022 | 0.0022 | 0.015 | 0.0013 | 0.0011 | 0.0004 | 0.0012 | 0.0007 | 0 | 0 |
Punctate Nuclear | 0.0175 | 0.0004 | 0.0015 | 0.0281 | 0.0018 | 0.0055 | 0.0048 | 0.0346 | 0.0026 | 0.0247 | 0.0149 | 0.0061 | 0.004 | 0.0275 | 0.0012 | 0.0013 | 0.0001 | 0.0108 |
Vacuole | 0.0044 | 0.0001 | 0.0003 | 0.0015 | 0.0065 | 0.0009 | 0.0006 | 0.0001 | 0.0018 | 0.0007 | 0.0035 | 0.0015 | 0.0006 | 0.0001 | 0.0002 | 0.0014 | 0.0003 | 0.0009 |
Vacuole Periphery | 0.0068 | 0.001 | 0.0018 | 0.004 | 0.016 | 0.0026 | 0.0038 | 0.0014 | 0.004 | 0.0024 | 0.0085 | 0.0065 | 0.0015 | 0.0005 | 0.0013 | 0.0032 | 0.0016 | 0.0019 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 177.1829 | 111.0987 | 167.6652 | 233.4561 | 267.2871 | 178.7633 | 136.2402 | 176.2218 | 251.2244 | 285.0979 |
Translational Efficiency | 1.3441 | 1.5851 | 1.2693 | 1.361 | 1.23 | 1.1809 | 1.4175 | 1.3182 | 1.3612 | 1.3066 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
193 | 189 | 1551 | 483 | 1391 | 1859 | 160 | 1222 | 1584 | 2048 | 1711 | 1705 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 3969.33 | 4041.01 | 2575.39 | 2181.95 | 3758.43 | 3074.37 | 3582.64 | 2515.89 | 3784.13 | 3163.58 | 2669.58 | 2421.29 |
Standard Deviation | 1563.85 | 1066.42 | 789.08 | 897.14 | 1061.71 | 907.71 | 946.54 | 760.33 | 1136.93 | 964.94 | 856.86 | 815.46 |
Intensity Change Log 2 | — | 0.025820 | -0.624105 | -0.863277 | — | -0.289839 | -0.069107 | -0.579061 | — | -0.119109 | -0.327581 | -0.718053 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000628 | 0.001077 | 0.001195 | 0.001570 | 0.001035 | 0.001061 | 0.001655 | 0.002044 | 0.000985 | 0.001062 | 0.001238 | 0.001910 |
Bud Neck | 0.003399 | 0.004773 | 0.008884 | 0.015144 | 0.001774 | 0.002240 | 0.008969 | 0.020900 | 0.001972 | 0.002474 | 0.008892 | 0.019269 |
Bud Site | 0.004066 | 0.000936 | 0.000711 | 0.002962 | 0.000400 | 0.000348 | 0.000682 | 0.001869 | 0.000847 | 0.000403 | 0.000708 | 0.002179 |
Cell Periphery | 0.000102 | 0.000045 | 0.000053 | 0.000168 | 0.000057 | 0.000074 | 0.000031 | 0.000120 | 0.000062 | 0.000072 | 0.000051 | 0.000134 |
Cytoplasm | 0.001512 | 0.000182 | 0.000726 | 0.004399 | 0.000107 | 0.000147 | 0.000060 | 0.001466 | 0.000278 | 0.000150 | 0.000664 | 0.002297 |
Cytoplasmic Foci | 0.057663 | 0.009434 | 0.002063 | 0.003678 | 0.008787 | 0.001643 | 0.000636 | 0.002778 | 0.014742 | 0.002362 | 0.001930 | 0.003033 |
Eisosomes | 0.000375 | 0.000180 | 0.000066 | 0.000051 | 0.000373 | 0.000291 | 0.000165 | 0.000044 | 0.000373 | 0.000281 | 0.000076 | 0.000046 |
Endoplasmic Reticulum | 0.000435 | 0.000111 | 0.000386 | 0.001313 | 0.000127 | 0.000211 | 0.000281 | 0.000837 | 0.000165 | 0.000202 | 0.000376 | 0.000972 |
Endosome | 0.000730 | 0.000107 | 0.000027 | 0.001045 | 0.000008 | 0.000006 | 0.000014 | 0.001053 | 0.000096 | 0.000016 | 0.000026 | 0.001051 |
Golgi | 0.000709 | 0.000658 | 0.000091 | 0.000451 | 0.000036 | 0.000020 | 0.000100 | 0.000817 | 0.000118 | 0.000079 | 0.000091 | 0.000714 |
Lipid Particles | 0.003439 | 0.000158 | 0.000013 | 0.000466 | 0.000046 | 0.000003 | 0.000020 | 0.000093 | 0.000459 | 0.000018 | 0.000013 | 0.000199 |
Mitochondria | 0.002084 | 0.001948 | 0.003074 | 0.005475 | 0.001944 | 0.001790 | 0.004872 | 0.008825 | 0.001961 | 0.001804 | 0.003242 | 0.007876 |
Mitotic Spindle | 0.020283 | 0.000161 | 0.000559 | 0.025323 | 0.000662 | 0.000148 | 0.001433 | 0.029101 | 0.003053 | 0.000149 | 0.000641 | 0.028031 |
None | 0.003368 | 0.001044 | 0.001042 | 0.004462 | 0.000783 | 0.000706 | 0.000606 | 0.001921 | 0.001098 | 0.000737 | 0.001001 | 0.002641 |
Nuclear Periphery | 0.000155 | 0.000067 | 0.001764 | 0.007248 | 0.000048 | 0.000154 | 0.000249 | 0.019894 | 0.000061 | 0.000146 | 0.001623 | 0.016311 |
Nuclear Periphery Foci | 0.004031 | 0.000028 | 0.000344 | 0.002350 | 0.000027 | 0.000044 | 0.000053 | 0.004663 | 0.000515 | 0.000042 | 0.000317 | 0.004008 |
Nucleolus | 0.487621* | 0.122919 | 0.189285 | 0.121274 | 0.095963 | 0.070350 | 0.205448 | 0.252287* | 0.143684 | 0.075201 | 0.190796 | 0.215173 |
Nucleus | 0.145116* | 0.832686* | 0.781156* | 0.794029* | 0.860998* | 0.912283* | 0.754252* | 0.639715* | 0.773773* | 0.904938* | 0.778640* | 0.683430* |
Peroxisomes | 0.262536* | 0.020945 | 0.005347 | 0.005870 | 0.025013 | 0.006317 | 0.019304 | 0.008019 | 0.053954 | 0.007667 | 0.006652 | 0.007410 |
Vacuole | 0.001074 | 0.000472 | 0.000675 | 0.001893 | 0.000575 | 0.000636 | 0.000520 | 0.001612 | 0.000636 | 0.000621 | 0.000661 | 0.001692 |
Vacuole Periphery | 0.000674 | 0.002069 | 0.002538 | 0.000830 | 0.001236 | 0.001529 | 0.000652 | 0.001941 | 0.001167 | 0.001579 | 0.002362 | 0.001626 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.02 | -4.99 | -8.12 | -3.56 | -5.77 | -0.46 | -4.51 | -10.69 | -11.59 | -2.09 | -1.18 | -4.24 | -12.33 | -12.68 | -10.20 |
Bud Neck | -0.45 | -5.72 | -8.39 | -6.25 | -6.17 | -2.34 | -8.21 | -15.77 | -15.48 | -10.79 | -1.63 | -15.55 | -18.23 | -17.76 | -12.63 |
Bud Site | 1.51 | 1.57 | 0.02 | -4.64 | -5.43 | 0.23 | -3.35 | -8.69 | -8.56 | -7.78 | 1.56 | 0.49 | -6.74 | -10.19 | -9.05 |
Cell Periphery | 1.39 | 1.28 | -0.24 | -7.05 | -7.50 | -0.90 | 1.20 | -5.06 | -4.33 | -7.04 | 0.01 | 0.88 | -5.00 | -5.84 | -8.36 |
Cytoplasm | 1.61 | 1.17 | -1.24 | -5.80 | -4.67 | -0.56 | 0.61 | -6.97 | -6.85 | -7.20 | 1.38 | -0.90 | -6.58 | -8.78 | -4.83 |
Cytoplasmic Foci | 4.79 | 6.63 | 6.42 | 0.94 | -0.83 | 4.30 | 5.04 | 2.58 | -2.40 | -3.70 | 6.51 | 6.57 | 5.57 | -1.62 | -1.74 |
Eisosomes | 4.44 | 8.34 | 8.67 | 5.25 | 3.04 | 1.94 | 6.47 | 11.01 | 8.13 | 9.71 | 2.43 | 11.22 | 12.29 | 8.48 | 9.16 |
Endoplasmic Reticulum | 1.30 | 0.26 | -2.96 | -6.10 | -4.87 | -5.18 | -3.87 | -9.22 | -8.19 | -5.88 | -0.84 | -5.28 | -9.51 | -9.77 | -7.46 |
Endosome | 1.43 | 1.52 | 0.30 | -1.87 | -2.07 | 0.48 | -1.62 | -1.95 | -1.96 | -1.93 | 1.47 | 1.25 | -2.09 | -2.58 | -2.52 |
Golgi | 0.25 | 2.87 | 1.10 | 0.20 | -3.97 | 2.59 | -3.13 | -3.53 | -3.60 | -3.19 | 0.83 | 0.58 | -3.67 | -3.84 | -3.84 |
Lipid Particles | 1.49 | 1.56 | 1.29 | -1.57 | -2.52 | 1.56 | 0.59 | -3.59 | -5.89 | -4.91 | 1.63 | 1.61 | 0.55 | -4.08 | -4.09 |
Mitochondria | 0.44 | -2.62 | -6.03 | -7.65 | -5.30 | 0.69 | -6.03 | -13.68 | -14.73 | -5.54 | 0.81 | -6.44 | -15.00 | -16.53 | -11.74 |
Mitotic Spindle | 2.83 | 2.74 | -0.97 | -5.59 | -5.46 | 1.29 | -0.57 | -8.73 | -8.94 | -8.15 | 3.07 | 2.28 | -8.99 | -10.55 | -10.26 |
None | 1.06 | 1.06 | -0.25 | -4.56 | -5.35 | 0.97 | 3.04 | -6.48 | -6.69 | -7.23 | 1.26 | 0.34 | -4.44 | -8.71 | -6.73 |
Nuclear Periphery | 2.44 | -2.75 | -3.13 | -3.19 | -2.23 | -2.96 | -3.15 | -7.03 | -6.98 | -6.94 | -2.53 | -2.97 | -7.66 | -7.61 | -6.47 |
Nuclear Periphery Foci | 1.66 | 1.55 | 0.96 | -2.91 | -2.41 | -0.82 | -1.27 | -6.28 | -6.26 | -6.24 | 1.62 | 0.81 | -4.50 | -6.89 | -6.14 |
Nucleolus | 15.41* | 14.84 | 17.16 | 0.13 | 5.85 | 4.43 | -4.63 | -15.62 | -19.51* | -1.92 | 10.20 | -5.57 | -7.35 | -17.86 | -1.86 |
Nucleus | -28.31* | -36.15 | -29.86 | 2.71 | 0.56 | -6.79 | 3.99 | 19.35 | 26.60 | 5.18 | -14.04 | -0.56 | 8.48 | 25.74 | 10.19 |
Peroxisomes | 15.81 | 17.85 | 17.88 | 3.10 | 0.59 | 8.12 | 1.75 | 8.00 | -1.02 | 3.86 | 13.81 | 14.32 | 14.52 | 0.97 | 0.24 |
Vacuole | 2.21 | 1.38 | -2.48 | -3.53 | -3.20 | -0.59 | -0.61 | -4.20 | -4.12 | -3.96 | 0.75 | -0.78 | -5.19 | -5.37 | -4.75 |
Vacuole Periphery | -1.25 | -3.61 | -0.40 | 1.20 | 3.48 | -0.63 | 1.39 | -0.83 | -0.32 | -3.45 | -0.96 | -1.98 | -0.52 | 0.68 | 1.92 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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