Standard name
Description Transcriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.08 0.08 0.11 0.06 0.1 0.09 0 0.1 0.06 0.24 0.22 0.26 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.09 0.2 0.16 0.12 0.18 0.16 0.21 0.19 0 0 0 0.21 0.2 0.14 0 0 0 0 0 0
Nucleus 0.89 0.92 0.92 0.85 0.62 0.42 0.37 0.37 0.35 0.36 0.44 0.49 0.91 0.85 0.82 0.55 0.52 0.51 0.85 0.82 0.68 0.79 0.68 0.37
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.35 0.2 0.15 0.23 0.33 0.57 0.58 0.55 0.51 0.57 0.47 0.44 0.06 0.08 0.14 0.22 0.23 0.2 0.12 0.12 0.24 0.07 0.16 0.36
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0.05 0.06 0.05 0.05 0 0 0 0 0 0.06 0.05 0 0 0 0.11 0.11 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 1 0 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 10
Bud Site 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 1 1 2 4 5 6 42 52 45 37 31 46 8 26 22 115 77 59 0 0 1 0 1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 2 2 0 0 0 0 0 18 20 7 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 2 5 1 0 0 0 1 0 0 0 1
Golgi 0 0 0 0 0 0 1 5 1 2 0 0 0 0 0 1 2 0 0 0 0 0 0 1
Mitochondria 3 3 5 5 22 60 88 78 74 96 68 93 3 1 2 99 71 32 0 0 0 0 3 3
Nucleus 66 69 195 159 158 127 201 245 148 212 143 245 170 230 295 263 182 117 74 72 153 118 191 98
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 1 2
Nucleolus 26 15 33 42 84 173 313 368 215 330 153 220 12 21 50 107 80 47 10 10 52 10 44 93
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0 2 1 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 2 0 0 0 0 0 1 6 0 0 0 1 0 0 1 2 12
Vac/Vac Membrane 1 1 0 0 12 5 19 35 27 29 16 22 1 3 13 20 21 12 0 1 10 16 29 31
Unique Cell Count 74 75 213 186 254 305 543 665 419 582 323 499 186 271 360 480 351 230 88 88 225 149 281 263
Labelled Cell Count 97 89 235 210 281 371 666 789 513 710 412 628 195 284 395 625 456 275 88 88 225 149 281 263


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 48.4 62.7 50.4 44.6 50.5 57.2 59.8 64.5 67.4 61.1 59.3 64.3 60.5 61.9 65.9 96.5 95.6 99.8 53.5 55.6 62.6
Std Deviation (1e-4) 17.6 20.1 17.8 14.1 16.2 20.4 24.3 28.5 30.4 25.2 25.0 26.2 24.2 29.7 30.6 48.0 48.4 47.1 21.3 23.0 23.7
Intensity Change (Log2) – – – -0.17 0.0 0.18 0.25 0.36 0.42 0.28 0.24 0.35 0.26 0.3 0.39 0.94 0.92 0.99 0.09 0.14 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 3.6 3.6 4.4 3.1 4.1 4.0 0 4.0 3.0 7.5 7.0 7.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.9 3.5 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.9 5.9 5.2 4.1 5.5 5.3 6.2 5.8 0 0 0 6.2 6.0 4.4
Nucleus -1.9 -7.4 -11.5 -13.5 -13.9 -13.4 -13.8 -11.1 -10.7 -0.1 -2.2 -3.2 -9.4 -9.7 -9.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.8 4.4 9.4 10.5 10.2 8.7 10.3 7.6 7.3 -2.8 -2.7 -0.5 2.1 2.1 1.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.2 0 2.8 3.4 3.8 3.3 3.3 3.1 0 0 0 3.0 3.6 3.4


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Wtm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available