Standard name
Human Ortholog
Description Transcriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.08 0.08 0.11 0.06 0.1 0.09 0 0.1 0.06 0.24 0.22 0.26 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.09 0.2 0.16 0.12 0.18 0.16 0.21 0.19 0 0 0 0.21 0.2 0.14 0 0 0 0 0 0
Nucleus 0.89 0.92 0.92 0.85 0.62 0.42 0.37 0.37 0.35 0.36 0.44 0.49 0.91 0.85 0.82 0.55 0.52 0.51 0.85 0.82 0.68 0.79 0.68 0.37
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.35 0.2 0.15 0.23 0.33 0.57 0.58 0.55 0.51 0.57 0.47 0.44 0.06 0.08 0.14 0.22 0.23 0.2 0.12 0.12 0.24 0.07 0.16 0.36
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0.05 0.06 0.05 0.05 0 0 0 0 0 0.06 0.05 0 0 0 0.11 0.11 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 1 0 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 10
Bud Site 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 1 1 2 4 5 6 42 52 45 37 31 46 8 26 22 115 77 59 0 0 1 0 1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 2 2 0 0 0 0 0 18 20 7 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 2 5 1 0 0 0 1 0 0 0 1
Golgi 0 0 0 0 0 0 1 5 1 2 0 0 0 0 0 1 2 0 0 0 0 0 0 1
Mitochondria 3 3 5 5 22 60 88 78 74 96 68 93 3 1 2 99 71 32 0 0 0 0 3 3
Nucleus 66 69 195 159 158 127 201 245 148 212 143 245 170 230 295 263 182 117 74 72 153 118 191 98
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 1 2
Nucleolus 26 15 33 42 84 173 313 368 215 330 153 220 12 21 50 107 80 47 10 10 52 10 44 93
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0 2 1 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 2 0 0 0 0 0 1 6 0 0 0 1 0 0 1 2 12
Vac/Vac Membrane 1 1 0 0 12 5 19 35 27 29 16 22 1 3 13 20 21 12 0 1 10 16 29 31
Unique Cell Count 74 75 213 186 254 305 543 665 419 582 323 499 186 271 360 480 351 230 88 88 225 149 281 263
Labelled Cell Count 97 89 235 210 281 371 666 789 513 710 412 628 195 284 395 625 456 275 88 88 225 149 281 263


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 48.4 62.7 50.4 44.6 50.5 57.2 59.8 64.5 67.4 61.1 59.3 64.3 60.5 61.9 65.9 96.5 95.6 99.8 53.5 55.6 62.6
Std Deviation (1e-4) 17.6 20.1 17.8 14.1 16.2 20.4 24.3 28.5 30.4 25.2 25.0 26.2 24.2 29.7 30.6 48.0 48.4 47.1 21.3 23.0 23.7
Intensity Change (Log2) -0.17 0.0 0.18 0.25 0.36 0.42 0.28 0.24 0.35 0.26 0.3 0.39 0.94 0.92 0.99 0.09 0.14 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 3.6 3.6 4.4 3.1 4.1 4.0 0 4.0 3.0 7.5 7.0 7.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.9 3.5 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.9 5.9 5.2 4.1 5.5 5.3 6.2 5.8 0 0 0 6.2 6.0 4.4
Nucleus -1.9 -7.4 -11.5 -13.5 -13.9 -13.4 -13.8 -11.1 -10.7 -0.1 -2.2 -3.2 -9.4 -9.7 -9.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.8 4.4 9.4 10.5 10.2 8.7 10.3 7.6 7.3 -2.8 -2.7 -0.5 2.1 2.1 1.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.2 0 2.8 3.4 3.8 3.3 3.3 3.1 0 0 0 3.0 3.6 3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 37.4471 39.975 36.2402 33.4635 29.7691 38.1988 48.3537 44.0136 35.3774 32.8182 26.7102 38.8294 52.0014 51.4676 42.473 38.1871 43.7437 45.2047
Actin 0.0188 0 0.0103 0.0002 0.0144 0.0003 0.0277 0 0.0107 0.0351 0.023 0.0069 0.001 0 0.0002 0.0003 0 0
Bud 0.0004 0.0001 0.0001 0.0001 0.0003 0.0002 0.0002 0 0.0004 0.0008 0.0005 0.0001 0.0001 0.0001 0 0.0005 0 0.0001
Bud Neck 0.0059 0 0.0005 0.0001 0.0004 0.0003 0.0002 0 0.0008 0.0004 0.0003 0.0002 0.0003 0 0 0.0002 0 0.0002
Bud Periphery 0.0005 0 0.0001 0.0002 0.0006 0.0002 0.0002 0 0.0004 0.0014 0.0004 0.0004 0.0001 0 0 0.0005 0.0001 0.0002
Bud Site 0.0041 0.0006 0.0076 0.0002 0.0007 0.0001 0.0004 0 0.0034 0.0019 0.0007 0.0001 0.0004 0.0002 0.0001 0.0005 0 0
Cell Periphery 0.0007 0.0001 0.0002 0.0004 0.0019 0.0005 0.0002 0.0001 0.0004 0.0001 0.0008 0.0006 0.0003 0 0.0001 0.0008 0.0002 0.0005
Cytoplasm 0.0044 0.0001 0.0003 0.0004 0.0015 0.0001 0.0017 0.0001 0.0004 0.0005 0.002 0.0006 0.0002 0.0001 0.0003 0.0004 0 0.0001
Cytoplasmic Foci 0.0161 0.0016 0.0026 0.0051 0.0061 0.0066 0.0067 0.0044 0.0158 0.0016 0.0134 0.021 0.0106 0.0239 0.0035 0.0255 0 0.0044
Eisosomes 0.0005 0 0.0001 0 0.0002 0 0.0002 0 0.0001 0.0002 0.0002 0.0001 0.0002 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0017 0 0.0003 0.0002 0.0031 0 0.0007 0 0.0002 0.0006 0.0008 0.0003 0.0001 0 0 0 0 0
Endosome 0.0162 0 0.002 0.0006 0.0386 0.0003 0.0045 0 0.0022 0.0051 0.0092 0.0029 0.005 0 0.0002 0.0001 0 0
Golgi 0.0036 0 0.0011 0.0002 0.0077 0.0002 0.0036 0 0.0035 0.0038 0.0112 0.0033 0.0014 0 0.0001 0.0001 0 0
Lipid Particles 0.0122 0.0001 0.0029 0.0053 0.0136 0.0029 0.0017 0.0002 0.0075 0.0016 0.0226 0.012 0.0032 0.0003 0.0033 0.0047 0 0.0004
Mitochondria 0.0097 0.0024 0.0044 0.0063 0.0206 0.0084 0.0135 0.0116 0.0119 0.0064 0.0463 0.0047 0.0071 0.0014 0.0035 0.0069 0.0041 0.0011
None 0.0183 0 0.0006 0.0014 0.0013 0.0001 0.0012 0.0001 0.0006 0.0003 0.0014 0.001 0.0006 0 0.0001 0.0003 0 0.0001
Nuclear Periphery 0.0188 0.0099 0.019 0.0113 0.0115 0.0028 0.0096 0.0026 0.0164 0.0294 0.0587 0.0167 0.0024 0.0051 0.0209 0.0093 0.0007 0.0012
Nucleolus 0.1301 0.0545 0.0484 0.0951 0.3104 0.1873 0.0895 0.0527 0.0638 0.05 0.2646 0.1672 0.109 0.0386 0.0427 0.0639 0.2805 0.1831
Nucleus 0.7061 0.9291 0.8947 0.8388 0.541 0.7803 0.8275 0.892 0.8509 0.8309 0.5019 0.7463 0.8508 0.9018 0.9211 0.8795 0.7122 0.795
Peroxisomes 0.003 0.0001 0.0011 0.0003 0.0018 0.0003 0.0013 0 0.0022 0.0022 0.015 0.0013 0.0011 0.0004 0.0012 0.0007 0 0
Punctate Nuclear 0.0175 0.0004 0.0015 0.0281 0.0018 0.0055 0.0048 0.0346 0.0026 0.0247 0.0149 0.0061 0.004 0.0275 0.0012 0.0013 0.0001 0.0108
Vacuole 0.0044 0.0001 0.0003 0.0015 0.0065 0.0009 0.0006 0.0001 0.0018 0.0007 0.0035 0.0015 0.0006 0.0001 0.0002 0.0014 0.0003 0.0009
Vacuole Periphery 0.0068 0.001 0.0018 0.004 0.016 0.0026 0.0038 0.0014 0.004 0.0024 0.0085 0.0065 0.0015 0.0005 0.0013 0.0032 0.0016 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 177.1829 111.0987 167.6652 233.4561 267.2871 178.7633 136.2402 176.2218 251.2244 285.0979
Translational Efficiency 1.3441 1.5851 1.2693 1.361 1.23 1.1809 1.4175 1.3182 1.3612 1.3066

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Transcriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats
Localization
Cell Percentages nucleus (76%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-1

Wtm1

Wtm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Wtm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available