Standard name
Human Ortholog
Description MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.99 0.96 0.98 0.9 0.77 0.79 0.68 0.65 0.62 0.66 0.99 1.0 1.0 0.96 0.98 0.95 0.96 0.93 0.87 0.92 0.91 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.09 0 0.28 0.4 0.38 0.54 0.61 0.64 0.64 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 4 1 1 1
Bud 0 3 2 1 0 1 2 4 6 5 4 7 0 0 0 0 0 0 0 2 0 0 7 6
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1
Bud Site 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 3 2 3 1 2 3 3 2 1 0 4 1 9 6 8 0 0 0 0 0 1
Cytoplasm 160 492 256 148 249 349 329 290 221 247 162 192 230 349 365 312 159 121 159 467 236 96 313 274
Endoplasmic Reticulum 1 3 1 2 0 0 3 1 4 2 4 0 2 0 1 27 13 12 0 3 2 0 1 1
Endosome 0 0 0 1 1 1 1 1 0 0 0 1 0 0 0 2 0 2 1 6 2 1 1 2
Golgi 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 5 3 0 1 1
Mitochondria 6 10 4 14 11 108 171 141 176 231 169 187 0 1 2 7 1 4 0 4 6 1 3 2
Nucleus 0 0 1 1 0 5 0 1 1 1 0 2 0 2 3 1 1 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 1 3 0 1 2 0 0 0 1 0 1 0 1 3 1 3 1
Vac/Vac Membrane 0 0 0 1 2 6 5 5 2 8 2 5 0 1 0 3 2 0 0 3 2 0 0 2
Unique Cell Count 166 502 258 154 255 389 427 368 326 379 263 291 232 350 366 324 162 127 167 500 271 105 343 302
Labelled Cell Count 168 511 264 171 265 473 516 447 416 497 344 401 232 357 372 365 184 148 167 500 271 105 343 302


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 5.2 4.6 4.4 4.3 3.9 3.2 3.7 3.3 3.0 3.3 3.1 5.3 5.4 5.5 5.8 6.8 6.9 4.8 5.4 6.0
Std Deviation (1e-4) 0.4 0.6 1.0 1.3 1.0 1.4 0.6 1.4 1.0 0.7 1.5 0.7 1.2 1.4 1.3 1.5 1.3 1.3 1.3 2.1 1.7
Intensity Change (Log2) – – – -0.07 -0.08 -0.25 -0.51 -0.32 -0.49 -0.61 -0.47 -0.57 0.21 0.24 0.25 0.33 0.56 0.59 0.07 0.24 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.2 -1.4 -4.8 -8.0 -7.5 -9.8 -10.4 -10.8 -10.0 -0.1 0.9 0.9 -2.3 -1.0 -2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 4.4 4.2 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1041 0.7021 0.3636 -0.154 -0.174 0.149 1.8414 2.1888 2.3091 2.0534 1.7006 2.0715 2.4905 2.7965 2.7281 2.7182 2.9597 2.7339
Actin 0.0419 0.0201 0.0051 0.0002 0.0003 0.0081 0.0379 0.0037 0.0129 0.0252 0.0198 0.0094 0.0973 0.0013 0.0161 0.0041 0.1415 0.0089
Bud 0.0004 0.0002 0.0001 0 0.0002 0.0003 0.0007 0.0012 0.0003 0.0006 0.01 0.0006 0.0007 0.0003 0.001 0.001 0.0007 0.0003
Bud Neck 0.0039 0.0003 0.0003 0.0001 0.0001 0.0029 0.0028 0.0002 0.0008 0.0002 0.0004 0.0052 0.0023 0.0002 0.0003 0.0002 0.0018 0.0004
Bud Periphery 0.0007 0.0001 0.0001 0 0.0002 0.0013 0.0015 0.0011 0.0006 0.0017 0.0194 0.001 0.0015 0.0006 0.0013 0.0024 0.0014 0.0006
Bud Site 0.0052 0.022 0.0006 0.0001 0.0001 0.0002 0.0067 0.0232 0.0006 0.0007 0.0021 0.0002 0.0027 0.0022 0.0036 0.0005 0.0049 0.0001
Cell Periphery 0.0005 0.0005 0.0003 0.0001 0.0001 0.0002 0.0018 0.0005 0.0001 0.0001 0.0011 0.0001 0.0011 0.0002 0.0002 0.0002 0.0003 0.0001
Cytoplasm 0.2623 0.3128 0.3675 0.4514 0.4179 0.5108 0.1308 0.318 0.1978 0.1445 0.2677 0.2861 0.203 0.4564 0.3205 0.3491 0.3456 0.4198
Cytoplasmic Foci 0.0063 0.0032 0.0137 0.0021 0.0072 0.0113 0.0269 0.0063 0.0127 0.0197 0.0043 0.0089 0.0268 0.0182 0.013 0.0072 0.025 0.011
Eisosomes 0.0011 0.0002 0.0002 0.0001 0.0001 0.0001 0.0004 0.0003 0.0003 0.0002 0.0004 0.0001 0.0009 0.0001 0.0003 0.0001 0.0027 0.0001
Endoplasmic Reticulum 0.0026 0.0029 0.0039 0.0032 0.0007 0.004 0.0042 0.0013 0.0019 0.0014 0.002 0.0013 0.0032 0.0023 0.0015 0.0065 0.0024 0.0013
Endosome 0.0069 0.0006 0.0114 0.0008 0.0005 0.0021 0.0297 0.0036 0.0101 0.0022 0.002 0.0046 0.0133 0.0024 0.0029 0.0052 0.0166 0.0015
Golgi 0.0126 0.0002 0.0043 0 0.0001 0.0004 0.0097 0.0007 0.0049 0.0045 0.0009 0.0032 0.0077 0.0003 0.001 0.0007 0.012 0.0007
Lipid Particles 0.0024 0.0005 0.0076 0.0001 0.0003 0.0005 0.0192 0.0018 0.0037 0.0047 0.0004 0.001 0.0162 0.001 0.0015 0.0018 0.033 0.0012
Mitochondria 0.0079 0.0002 0.0045 0 0.0002 0.0038 0.0114 0.0003 0.0021 0.0009 0.0301 0.0008 0.0033 0.0001 0.0004 0.0009 0.0076 0.0004
None 0.6387 0.6328 0.558 0.5391 0.5704 0.4486 0.66 0.6331 0.7442 0.7554 0.6289 0.6718 0.5861 0.5099 0.6267 0.608 0.3435 0.5449
Nuclear Periphery 0.0006 0.0006 0.0022 0.0007 0.0002 0.0006 0.0083 0.0006 0.001 0.0004 0.003 0.0007 0.0029 0.0005 0.0004 0.0038 0.0193 0.0005
Nucleolus 0.0002 0.0001 0.0028 0.0001 0.0001 0.0001 0.0018 0.0001 0.0002 0.0001 0.0003 0.0001 0.0008 0.0001 0.0001 0.0003 0.0009 0.0001
Nucleus 0.0006 0.0008 0.01 0.0009 0.0004 0.0017 0.0036 0.0008 0.0005 0.0006 0.0029 0.001 0.0014 0.0008 0.0006 0.0018 0.0017 0.0006
Peroxisomes 0.0037 0.0007 0.0034 0 0.0002 0.0005 0.0318 0.0014 0.0031 0.0348 0.0017 0.0021 0.0206 0.0003 0.0072 0.0015 0.0281 0.0025
Punctate Nuclear 0.0006 0.0005 0.0025 0.0003 0.0004 0.002 0.0074 0.0012 0.0011 0.0017 0.0009 0.0013 0.0062 0.0021 0.0009 0.0024 0.0089 0.0047
Vacuole 0.0007 0.0006 0.0012 0.0006 0.0004 0.0005 0.0023 0.0004 0.0008 0.0003 0.0012 0.0005 0.0016 0.0006 0.0004 0.0018 0.001 0.0003
Vacuole Periphery 0.0002 0 0.0004 0 0 0.0001 0.0012 0.0001 0.0003 0.0001 0.0006 0.0001 0.0004 0.0001 0.0001 0.0006 0.0011 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.9553 16.1489 15.3385 19.7029 15.4585 23.8325 22.5329 21.1592 21.9748 21.6287
Translational Efficiency 0.6088 0.8516 0.6984 0.7017 0.7329 0.7065 0.5034 0.6084 0.6363 0.5544

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1761 1315 231 2289 1932 1301 2346 113 3693 2616 2577 2402

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 734.77 731.54 895.08 904.39 703.06 733.10 856.16 974.33 718.18 732.32 859.65 907.68
Standard Deviation 75.08 97.63 125.18 120.94 108.29 97.15 96.11 112.18 95.26 97.39 99.69 121.45
Intensity Change Log 2 — -0.006356 0.284724 0.299652 — 0.060362 0.284233 0.470763 — 0.026653 0.284484 0.385857

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000052 0.000350 0.000503 0.001577 0.000072 0.000557 0.000084 0.000905 0.000062 0.000453 0.000121 0.001545
Bud Neck 0.007673 0.024458 0.001434 0.016747 0.007862 0.029169 0.005327 0.003016 0.007772 0.026801 0.004978 0.016101
Bud Site 0.003333 0.018077 0.003138 0.054995 0.002522 0.015281 0.009487 0.122935* 0.002909 0.016686 0.008918 0.058191
Cell Periphery 0.000067 0.000122 0.000251 0.000294 0.000108 0.000166 0.000104 0.000159 0.000088 0.000144 0.000117 0.000288
Cytoplasm 0.661225* 0.511976* 0.391427* 0.534847* 0.586006* 0.498518* 0.649336* 0.436595* 0.621874* 0.505283* 0.626217* 0.530225*
Cytoplasmic Foci 0.084645 0.191863* 0.023580 0.014526 0.145896 0.228272* 0.012983 0.033339 0.116688 0.209970* 0.013933 0.015411
Eisosomes 0.000053 0.000096 0.000076 0.000040 0.000104 0.000136 0.000028 0.000071 0.000080 0.000116 0.000033 0.000041
Endoplasmic Reticulum 0.000336 0.000794 0.005927 0.001469 0.000673 0.000449 0.000746 0.002499 0.000513 0.000622 0.001210 0.001517
Endosome 0.003811 0.023040 0.004841 0.004675 0.006185 0.022796 0.001438 0.015576 0.005053 0.022919 0.001743 0.005188
Golgi 0.000550 0.003100 0.000112 0.002371 0.001243 0.004279 0.000070 0.011733 0.000913 0.003686 0.000074 0.002811
Lipid Particles 0.001495 0.004621 0.002364 0.000704 0.004422 0.005091 0.000561 0.002157 0.003026 0.004855 0.000723 0.000773
Mitochondria 0.000893 0.004468 0.000267 0.005569 0.001447 0.005257 0.000529 0.004902 0.001183 0.004860 0.000506 0.005538
Mitotic Spindle 0.001494 0.002739 0.000271 0.050850 0.003527 0.005055 0.008960 0.012836 0.002557 0.003890 0.008181 0.049062
None 0.006749 0.004142 0.002755 0.006121 0.007244 0.002342 0.003872 0.006681 0.007008 0.003247 0.003772 0.006147
Nuclear Periphery 0.000167 0.000512 0.001304 0.001114 0.000389 0.000242 0.000871 0.001053 0.000283 0.000377 0.000910 0.001111
Nuclear Periphery Foci 0.000248 0.000924 0.004723 0.000819 0.000414 0.000494 0.000445 0.001741 0.000335 0.000710 0.000828 0.000862
Nucleolus 0.000754 0.001345 0.000430 0.000728 0.000924 0.001635 0.000319 0.000149 0.000843 0.001489 0.000329 0.000701
Nucleus 0.169722* 0.100392 0.418723* 0.207510* 0.186268* 0.072915 0.230553* 0.148689* 0.178378* 0.086727 0.247421* 0.204743*
Peroxisomes 0.000898 0.007314 0.000343 0.001903 0.001888 0.011264 0.000120 0.000576 0.001416 0.009278 0.000140 0.001840
Vacuole 0.055538 0.097874 0.137339 0.090356 0.042315 0.094472 0.074019 0.193233 0.048620 0.096183 0.079695 0.095195
Vacuole Periphery 0.000296 0.001794 0.000192 0.002785 0.000490 0.001611 0.000147 0.001155 0.000398 0.001703 0.000151 0.002708

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.95 -7.68 -5.03 -2.99 -1.43 -4.38 -3.86 -3.56 -0.02 -3.22 -6.68 -8.56 -5.11 -2.37 -4.45
Bud Neck -8.52 7.36 -3.33 6.55 -11.09 -9.16 3.30 5.52 11.31 2.38 -12.50 4.29 -3.46 9.65 -6.70
Bud Site -8.67 -1.09 -10.74 -3.11 -10.53 -9.54 -3.92 -1.94 -0.90 -1.58 -12.75 -3.64 -11.26 -4.15 -8.62
Cell Periphery -7.32 -7.54 -8.95 -4.66 1.85 -1.37 2.21 -2.01 0.64 -3.13 -2.65 -2.87 -7.50 -2.06 -5.87
Cytoplasm 15.87 18.16 29.77 8.19 -5.28 8.94 3.41 9.60 6.31 8.60 17.09 12.23 28.58 9.90 14.14
Cytoplasmic Foci -17.37 17.59 24.80 33.30 4.11 -11.73 35.16 26.06 31.80 -1.10 -19.73 41.22 41.46 49.31 2.03
Eisosomes -7.12 -5.39 0.53 8.36 6.08 -2.93 12.58 2.02 4.04 -5.01 -5.93 11.10 7.83 11.98 -5.37
Endoplasmic Reticulum -5.59 -10.46 -17.90 -11.20 7.86 1.66 -4.15 -5.51 -6.21 -4.35 -1.52 -11.47 -14.82 -15.65 -2.70
Endosome -11.69 -1.99 3.81 12.96 5.11 -10.98 9.09 1.08 7.40 -1.21 -15.96 9.70 6.72 18.82 -1.26
Golgi -5.87 7.72 -0.50 4.42 -1.85 -4.55 4.65 -0.37 1.21 -1.02 -6.94 6.22 0.42 5.92 -2.22
Lipid Particles -5.55 -4.10 2.56 6.19 5.48 -0.69 10.58 6.69 3.76 -4.74 -3.31 10.87 9.28 7.05 -5.45
Mitochondria -4.02 5.21 -2.67 2.05 -3.88 -3.48 3.24 0.50 3.27 -0.80 -5.21 4.06 -2.05 3.01 -3.53
Mitotic Spindle -1.36 1.44 -9.10 -8.72 -10.04 -0.81 -2.72 -1.98 -1.87 -1.63 -1.29 -3.64 -8.93 -8.26 -6.39
None 2.32 5.54 1.35 -1.72 -8.30 4.09 3.08 0.04 -3.34 -2.46 4.52 4.71 1.76 -4.37 -5.74
Nuclear Periphery -2.36 -15.01 -27.98 -10.33 -4.13 3.24 -16.88 -8.31 -9.29 -2.01 -1.25 -23.41 -26.43 -17.64 -9.15
Nuclear Periphery Foci -4.21 -7.71 -9.15 0.52 6.76 -1.02 -1.14 -6.16 -5.39 -5.70 -4.09 -6.89 -8.27 -1.45 0.38
Nucleolus -3.16 2.74 1.89 4.94 -0.80 -1.78 4.29 4.19 3.29 0.59 -2.83 5.35 3.18 4.38 -1.73
Nucleus 13.25 -12.71 -18.92 -29.10 6.79 22.95 -14.92 -0.33 -5.68 3.96 25.32 -20.97 -18.21 -35.65 2.03
Peroxisomes -10.60 5.16 0.97 10.71 -2.45 -8.90 8.14 7.26 10.71 -1.77 -12.95 10.34 3.73 14.03 -3.95
Vacuole -9.77 -9.33 -25.59 -15.14 -0.02 -13.99 -19.73 -10.79 -9.04 -8.28 -16.67 -20.40 -29.92 -18.76 -12.55
Vacuole Periphery -8.38 3.86 -1.90 2.51 -2.21 -4.87 4.18 1.24 4.99 -0.82 -8.94 5.58 -1.52 2.56 -2.48
Standard name
Human Ortholog
Description MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (75%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mkk1

Mkk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mkk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available